Terroir 2020 banner
IVES 9 IVES Conference Series 9 International Terroir Conferences 9 Terroir 2020 9 History and innovation of terroir 9 Spectral characterisation of fungal diseases on Vitis vinifera leaves

Spectral characterisation of fungal diseases on Vitis vinifera leaves

Abstract

Aims: The aims of this study were to (1) detect alterations in the reflectance spectra of vines with fungal diseases, (2) map these alterations, and (3) determine the best wavelengths which may be used as early indicators of fungal diseases in vines.

Methods and Results: Cabernet Sauvignon vines grown in pots and kept in a greenhouse were inoculated with the pathogens causing mildew, powdery mildew, black-foot and Petri disease. In early stages of disease development, reflectance measurements were performed using a FieldSpec 3 spectroradiometer, which were compared with data from healthy plants. Additional measurements were performed with chlorophyll meters. The investigation began with discriminant analysis, which revealed that symptomatic plants are indeed separated from the control ones. Reflectance spectra were therefore further investigated, looking for alterations on the shape of the spectra, characteristic of each disease. The disease descriptors were based on ratios between spectral features internal to a spectrum, a procedure which allowed the derivation of parameters intrinsic to each disease. A set of thresholds, defined as the intensity ratios of reflectance at selected wavelengths, was derived for the studied diseases. The selected wavelength ratios were 443/496, 443/573, 443/695, 443/1900, 496/573, 496/695, 516/1900, and 1900/2435 (values in nanometers), for which the spectra from symptomatic plants present shape changes of as much as 20% with respect to healthy plants.

Conclusions:

Spectral deformations were observed for the studied fungal diseases; they are larger for black-foot and powdery mildew, but some wavelength ratios are also indicators of downy mildew and Petri disease. Data from near-infrared in general carry more information compared with measurements at 1900 and 2435nm.

Significance and Impact of the Study: Since little is known on alterations of the reflectance spectra of vines, a better knowledge could be used in the development of sensors able to detect diseases through fast, non-destructive techniques. Early disease detection can lead to preventive actions which potentially can mitigate losses in grape yield and quality.

DOI:

Publication date: March 23, 2021

Issue: Terroir 2020

Type: Video

Authors

Pâmela A. Pithan1, Jorge R. Ducati1*, Lucas R. Garrido2

1Remote Sensing Center, Universidade Federal do Rio Grande do Sul, Av. Bento Goncalves 9500, 91501-970 Porto Alegre RS, Brazil
2Centro Nacional de Pesquisas em Uva e Vinho, EMBRAPA, Bento Goncalves RS, Brazil

Contact the author

Keywords

Grapevine diseases, leaf reflectance, spectroradiometry, disease detection

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Spatial Analysis of Climate in Winegrape Growing Regions in Portugal

Spatial climate data at a 1 km resolution has allowed for a comprehensive mapping and assessment of viticulture DOs regions in Portugal. Overall the 50 regions and sub-regions in Portugal range

Yield formation and grape composition: more than meets the eye 

Fruit quality in grapes is not well defined but is often depicted as correlating inversely with crop yield. Both fruit yield and composition, however, are made from distinct components that interact in complex ways. Reproductive growth of grapevines extends over two growing seasons. Inflorescences initiated in buds during the previous year differentiate flowers and set and develop berries during the harvest year.

Influence of toasting oak wood on ellagitannin structures

Ellagitannins (ETs) have been reported to be the main phenolic compounds found in oak wood. These compounds, belonging to the hydrolysable tannin class of polyphenols, are esters of hexahydroxydiphenic acid (HHDP) and a polyol, usually glucose or quinic acid. They own their name to their capacity to be hydrolysed and liberate ellagic acid and they have an impact on astringency and bitterness sensation, which is strongly dependant on their structure. The toasting phase is particularly crucial in barrels fabrication and influences wood composition.

Under-vine management effects on grapevine production, soil properties and plant communities in South Australia

Under-vine (UV) management has traditionally consisted of synthetic herbicide use to limit competition between weeds and grapevines. With growing global interest towards non-synthetic chemical use, this study aimed to capture the effects of alternative UV management at two commercial Shiraz vineyards in South Australia, where the sole management variables were UV management since 2016. In adjacent treatment blocks, cultivation (CU) was compared to spontaneous vegetation (SV) in McLaren Vale (MV), and herbicide was compared to SV in Eden Valley (EV). Soil water infiltration rates were slower and grapevine stem water potential was lower in CU compared to SV in MV, with the latter having a plant community dominated by soursob (Oxalis pes-caprae) during winter; while in EV, there was little separation between the treatments. Yields were affected at both sites, with SV being higher in MV and HE being higher in EV. In MV, the only effect on grape must was a lower 13C:12C isotope ratio in CU, indicating greater grapevine water stress. In the grape must at EV, SV had higher total soluble solids, total phenolics, anthocyanins, and yeast available nitrogen; and lower pH and titratable acidity. Pruning weights were not affected by the treatments in MV, while they were higher in HE at EV. Assessments revealed that the differing soil types at the two sites were likely the main determinants of the opposing production outcomes associated with UV management. In the silty loam soil of MV, the higher yields in SV were likely due to more plant-available water, as a potential result of the continuous soil bio-pores formed by winter UV vegetation. Conversely, in the loamy sand soils of EV with a lower cation exchange capacity, the lower yields and pruning weights in SV suggest the UV vegetation competed significantly with the grapevines for available water and nutrients.

Assessment of climate change impacts on water needs and growing cycle on grapevine in three DOs of NE Spain

This study assessed the suitability of grapevine growing in three DOs (Empordà, Pla de Bages and Penedès) of Catalonia (NE Spain) over the 21st century. For this purpose, an estimation of water needs and agroclimatic and phenological indicators was made. Climate change impacts were estimated at 1 km pixel resolution using temperature and precipitation projections from several general circulation models (GCM) and two climate change scenarios: RCP 4.5 (stabilization scenario) and RCP 8.5 (worst-case scenario). Potential crop evapotranspiration (following FAO procedure) and a daily water balance considering soil water holding capacity were used to estimate actual evapotranspiration of vines and, finally, water needs. Dynamics would be similar in the three DOs studied although the magnitude of impact differs. Water needs would be 2 and 3 times greater (ranging from 0 to more than 1500 m3/ha) than current water needs at both climate change scenarios. Moreover, blooming date would advance from 3 to 6 weeks, harvest date from 1 to 2.5 months, resulting in growing cycles from 10 to 80 days shorter. It should also be noted that frost risk would decrease from 6 to 76%, the number of days with temperatures above 30ºC during ripening would rise from 48 to 500% and tropical nights (minimum temperature >20ºC) at ripening would increase from 28 to 150%, depending on the scenario and the DOs. The impacts of climate change in the three DOs could result in significant limitations for grapevine cultivation and wine production if adaptive strategies are not applied. This result could serve as a basis for the design of specific and particular adaptation strategies to improve and maintain vineyards in the DOs studied and could be extrapolated to similar DOs and regions.