Terroir 2020 banner
IVES 9 IVES Conference Series 9 International Terroir Conferences 9 Terroir 2020 9 History and innovation of terroir 9 Spectral characterisation of fungal diseases on Vitis vinifera leaves

Spectral characterisation of fungal diseases on Vitis vinifera leaves

Abstract

Aims: The aims of this study were to (1) detect alterations in the reflectance spectra of vines with fungal diseases, (2) map these alterations, and (3) determine the best wavelengths which may be used as early indicators of fungal diseases in vines.

Methods and Results: Cabernet Sauvignon vines grown in pots and kept in a greenhouse were inoculated with the pathogens causing mildew, powdery mildew, black-foot and Petri disease. In early stages of disease development, reflectance measurements were performed using a FieldSpec 3 spectroradiometer, which were compared with data from healthy plants. Additional measurements were performed with chlorophyll meters. The investigation began with discriminant analysis, which revealed that symptomatic plants are indeed separated from the control ones. Reflectance spectra were therefore further investigated, looking for alterations on the shape of the spectra, characteristic of each disease. The disease descriptors were based on ratios between spectral features internal to a spectrum, a procedure which allowed the derivation of parameters intrinsic to each disease. A set of thresholds, defined as the intensity ratios of reflectance at selected wavelengths, was derived for the studied diseases. The selected wavelength ratios were 443/496, 443/573, 443/695, 443/1900, 496/573, 496/695, 516/1900, and 1900/2435 (values in nanometers), for which the spectra from symptomatic plants present shape changes of as much as 20% with respect to healthy plants.

Conclusions:

Spectral deformations were observed for the studied fungal diseases; they are larger for black-foot and powdery mildew, but some wavelength ratios are also indicators of downy mildew and Petri disease. Data from near-infrared in general carry more information compared with measurements at 1900 and 2435nm.

Significance and Impact of the Study: Since little is known on alterations of the reflectance spectra of vines, a better knowledge could be used in the development of sensors able to detect diseases through fast, non-destructive techniques. Early disease detection can lead to preventive actions which potentially can mitigate losses in grape yield and quality.

DOI:

Publication date: March 23, 2021

Issue: Terroir 2020

Type: Video

Authors

Pâmela A. Pithan1, Jorge R. Ducati1*, Lucas R. Garrido2

1Remote Sensing Center, Universidade Federal do Rio Grande do Sul, Av. Bento Goncalves 9500, 91501-970 Porto Alegre RS, Brazil
2Centro Nacional de Pesquisas em Uva e Vinho, EMBRAPA, Bento Goncalves RS, Brazil

Contact the author

Keywords

Grapevine diseases, leaf reflectance, spectroradiometry, disease detection

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Agronomic and oenological behavior of the minority Mandón variety on two rootstocks in the D.O. Arribes

A large population of vines of the Mandón minority red variety (synonymous with Garró) has been located in old vineyards of the D.O. Arribes (Zamora and Salamanca) to conserve and recover this minority variety. The wines made with this variety are characterized by their good structure and color, interesting harmony, an excellently low pH, with high acidity, as well as complex aromas of blue fruits and a marked and expressive minerality.

Biomass carbon and nitrogen input from cover crops in an irrigated vineyard in Okanagan Valley, Canada

The use of cover crops in vineyards has been encouraged by positive effects on wine grape yield and sensory attributes, and improved soil function. This study examined the efficacy of three alleyway and three undervine cover crop treatments in an organic vineyard in the semiarid Okanagan Valley, Canada in 2021.

IMPACT OF MINERAL AND ORGANIC NITROGEN ADDITION ON ALCOHOLIC FERMENTATION WITH S. CEREVISIAE

During alcoholic fermentation, nitrogen is one of essential nutrient for yeast as it plays a key role in sugar transport and biosynthesis of and wine aromatic compounds (thiols, esters, higher alcohols). The main issue of a lack in yeast assimilable nitrogen (YAN) in winemaking is sluggish or stuck fermentations promoting the growth of alteration species and leads to economic losses. Currently, grape musts are often characterized by low YAN concentration and an increase of sugars concentration due to global warming, making alcoholic fermentations even more difficult. YAN depletion can be corrected by addition of inorganic (ammonia) or organic (yeast derivatives products) nitrogen during alcoholic fermentation.

Biodiversity and biocontrol ability of Trichoderma natural populations in soil vineyards from Castilla y León region (Spain)

Trichoderma is a microorganism present in many agricultural soils and some of its species could be used as natural biological control agents. In this work, the presence of natural populations of Trichoderma was estimated in soil vineyard and its biocontrol capacity against Phaeoacremonium minimum, one of the main agent causals of grapevine trunk diseases instead of using pesticides. Moreover, physicochemical variables in soil such as pH, organic matter and nutrients were evaluated to determine a possible correlation to natural populations of Trichoderma.

Organic recycled mulches in sustainable viticulture: assessment of spontaneous plants communities and weed coverage

In recent years, developing more efficient and sustainable viticulture management has been essential due to the impact of climate change in semiarid regions. For this reason, the use of recycled organic mulching (ROM) in the vineyard has become an interesting strategy to cope with water stress, isolated soil from extreme temperatures and improving soil humidity, control the presence of weeds and therefore reduce the inputs of herbicides and improve soil fertility. This work aimed to analyse the effect of three different organic mulches [straw (S), grape pruning debris (GPD) and spent mushroom compost (SMC)] and two traditional soil management techniques [herbicide (H) and interrow (IN)] on weed coverage and the spontaneous plant communities’ presence. Data sampling was collected throughout the vine vegetative cycle of 2021 in La Rioja, Spain. The different soil management techniques had a clear effect on weed coverage and his development during the vine vegetative cycle. SMC and H were the treatments with the highest and the lowest coverage percentage, respectively. IN had a delayed weed emergence at the beginning of the vine vegetative cycle, but finally it reached maximum values nearby SMC. GPD and S had similar effects on weed emergence, reaching 25-30% of the maximum coverage values. A total of 29 herbaceous species were identified during the vegetative cycle, some of them very isolated and occasional. Principal component analysis (PCAs) showed a good association between spontaneous species and treatments, furthermore, specific species-treatment associations were found. Moreover, three clear groups of herbaceous communities were identified by cluster analysis. This study provides interesting information about the effect of different alternative soil management on herbaceous plant coverage and weed species communities which could contribute to making more sustainable viticulture.