Terroir 2020 banner
IVES 9 IVES Conference Series 9 Evaluation of six red grapevine cultivars inoculated with Neofusicoccum parvum in a “terroir” of La Mancha wine región (Spain)

Evaluation of six red grapevine cultivars inoculated with Neofusicoccum parvum in a “terroir” of La Mancha wine región (Spain)


Aim: Among Botryosphaeriaceae species associated with Botryosphaeria dieback of grapevines, Neofusicoccum parvum is one of the most virulent and fastest wood-colonizing fungi. This study aimed to evaluate the susceptibility of six red grapevine cultivars (“Bobal”, “Monastrell”, “Garnacha Tinta”, “Moravia Agria”, “Tinto Velasco” and “Moribel” to N. parvum, under field conditions.

Methods and Results: Pathogenicity studies were conducted, over two consecutive years, with one-year old grapevine rooted cuttings inoculated with a N. parvum isolate. Rooted cuttings were wounded between the two upper internodes with a cork borer. A mycelial agar plug, from a 3-weeks-old culture on potato dextrose agar (PDA), was placed in the wound. Wounds were sealed with parafilm and wrapped with foil paper to prevent drying. Ten rooted cuttings per cultivar were inoculated with the fungus and two others with uncolonized PDA plugs, as negative controls. After inoculating, rooted cuttings were planted in a plot and irrigated by a drip system with two drippers per plant. Plants were collected after eight months and inspected for lesion development. Extent of wood necrosis was measured upward and downward from the inoculation point. Three rooted cuttings for each cultivar were selected and small pieces, of necrotic tissue from de edge of each lesion, were cut and placed on malt extract agar supplemented with 0.5 g/L of streptomycin sulphate (MEAS), in an attempt to recover the inoculated fungus and complete Koch’s postulates. N. parvum was identified by morphological and molecular approaches. Mean percentage of infected rooted cuttings ranged from 42.1% (“Monastrell” cultivar) to 93.3% (“Tinto Velasco” cultivar). Mean lengths of the extent of wood necrosis caused by N. parvum on inoculated one-year-old grapevine wood ranged from 21.2 (“Bobal” cultivar) to 87.2 mm (“Tinto Velasco” cultivar). N. parvum was reisolated from the edge of each lesion in 90.3% of the cultivars. The results of statistical analysis showed that “Bobal” and “Monastrell” cultivars were significantly more tolerant than “Tinto Velasco”.  


All tested grapevine cultivars were susceptible to infection by N. parvum, evidencing that there was no qualitative resistance to this fungus. “Bobal” and “Monastrell” cultivars highlighted for their lower wood response susceptibility to N. parvum.

Significance and Impact of the Study: Interactions between Botryosphaeriaceae species and grapevine cultivars are poorly understood and there is currently little data available. This study allowed classifying different Vitis vinifera cultivars based on their degree of quantitative resistance to N. parvum. “Bobal” and “Monastrell” cultivars could be potential candidates to create tolerant varieties to N. parvum fungus. Using tolerant varieties would be the safest, easiest, the least expensive and the most effective means of controlling this disease.


Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video


Juan Luis Chacón1*, David Gramaje2, Adela Mena1, Pedro Miguel Izquierdo1, Jesús Martínez1

1Instituto Regional de Investigación y Desarrollo Agroalimentario y Forestal de Castilla-La Mancha (IRIAF), Ctra. Toledo-Albacete s/n, 13700 Tomelloso, Spain
2Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas – Universidad de la Rioja – Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, Spain

Contact the author


Botryosphaeria dieback, grapevine, grapevine trunk diseases, Neofusicoccum parvum


IVES Conference Series | Terroir 2020


Related articles…

Significance of factors making Riesling an iconic grape variety

Riesling is the iconic grape variety of Germany and accounts for 23% of the German viticulture acreage, which comprises 45% of the worldwide Riesling plantings. Riesling wines offer a wide array of styles from crisp sparkling wines to highly concentrated and sweet Trockenbeerenauslese or Icewines. However, its thin berry skin makes Riesling more vulnerable to detrimental environmental threats than other white wine varieties.  

Malolactic fermentation in wine production

What influence do these bacteria have on wines? What new bacteria are being studied to carry out this fermentation? Find below articles about malolactic fermentation published in our 3 media (OENO One, IVES Technical Reviews and IVES Conference Series). OENO One...

Metabolomics screening of Vitis sp. interspecific hybrids to select natural ingredients with cosmetic purposes

Introducing natural ingredients using green chemistry practices is a major challenge in cosmetics industry to follow the market trend. Among the plants of cosmetic interest, vine products show a remarkable diversity of natural substances with high potential for the cosmetic and dermatological sectors. To date, research focuses on well-known compounds like E-resveratrol and E-ε-viniferin,

WAC 2022: Abstracts are available on IVES Conference Series

In order to disseminate the scientific results presented during the WAC 2022 , the organizers have decided to share the abstracts of the oral communications and posters with Open Access on IVES Conference Series. The fifth edition of the International Conference...

Winemaking techniques and wine tasting methods at the end of the Middle Ages

Les pratiques de vinification et de dégustation du vin sont souvent perçues, à travers un discours marketing très puissant, sous l’angle d’une tradition millénaire qui perdure depuis le Moyen Âge. En Bourgogne, il est courant de rattacher les racines de ces pratiques à l’activité des institutions ecclésiastiques qui possédaient d