Enoforum 2021
IVES 9 IVES Conference Series 9 Enoforum Web 9 Enoforum Web Conference 2021 9 Cellar session 9 Effect of Candida zemplinina oak chips biofilm on wine aroma profile

Effect of Candida zemplinina oak chips biofilm on wine aroma profile

Abstract

Candida zemplinina (synonym Starmerella bacillaris) is frequently isolated in grape must in different vitivinicultural areas. The enological significance of C. zemplinina strains used in combination with S. cerevisiae has been demonstrated, being wines produced by the above-mixed starter, characterized by higher amounts of glycerol and esters. The ability of this species to compete in a harsh environment such as wine is due to elaborate survival strategies. Biofilm formation is the principal way of resisting environmental stresses and represent the main microbial lifestyle in natural niches. Therefore, in this study 10 strains of C. zemplinina were analyzed to assess cell surface hydrophobicity using microbial adhesion to solvents (MATS) test and tested for their ability to form biofilms on winemaking material such as stainless steel and oak chips. The contribution of C. zemplinina biofilm on this material to wine aroma was evaluated. All strains showed a certain degree of hydrophobicity, and adhered to tested surfaces. In particular, sessile cells on chips ranged from 4.3 Log CFU/mL to 6 Log CFU/mL, while on stainless steel from 2.6 CFU/mL to 4.2 CFU/mL. Solid-phase microextraction gas chromatography-mass spectrometry showed that biofilm developed on oak can modulate the wood-wine transfer of volatile aromatic compounds. Therefore, surface-associated behaviours should be considered in the development of improved strategies to shape aroma profile of wines.

DOI:

Publication date: April 23, 2021

Issue: Enoforum 2021

Type: Article

Authors

Giorgia Perpetuini, Noemi Battistelli, Alessio Pio Rossetti, Giuseppe Arfelli, Rosanna Tofalo

Faculty of BioScience and Technology for Food, Agriculture and Environment, University of Teramo – Via R. Balzarini, 1, 64100, Teramo, Italy

Contact the author

Tags

Enoforum 2021 | IVES Conference Series

Citation

Related articles…

Clone performance under different environmental conditions in California

Clonal evaluation of winegrapes in California has not been extensive. Early selection work by Alley (1977), Olmo (unpublished data) and Goheen (personal communication) resulted in the current collection

Mineral-wine profile and AI: wine authentication and identification

Enhancing the mineral wine profile: from authentication to identification by artificial intelligence for enhanced security. Analysis of a wine’s mineral concentration profile provides a distinctive fingerprint for each cuvée. Unlike organic profiles, this identification signature remains stable over time and can be deciphered using direct analysis by inductively coupled mass spectrometry (icp-ms).

Monitoring small-scale alcoholic fermentations using a portable FTIR-ATR spectrometer and multivariate analysis

Although some wine production processes still rely on post-production evaluation and off-site laboratory analysis, the new winemaking industry is aware of a need for a better knowledge of the process to improve the properties of the final product. Thus, more and more wineries are interested in incorporating quality-by-design (QbD) strategies instead of postproduction testing because of the possibility to early detect deviations in fermentation or any other wine process. This would allow to detect unwanted situations and eventually to ‘readjust’ the process, thus minimizing rejects.

A versatile genome editing platform for grapevine: improving biotic and abiotic stress resilience 

New Plant Breeding Techniques (NPBTs) have arisen with the objective of surmounting the constraints inherent in conventional breeding methodologies, thereby enhancing plant resilience against both biotic and abiotic stresses. To date the application of genome editing in grapevine is still limited by the necessity to overcome recalcitrance to produce embryogenic calli and to regenerate plants. In our studies, we developed a smart and versatile genetic transformation system carrying all the most promising features of different genome editing approaches. In specific, we joined the GRF-GIF expression to improve regeneration, the systemic movement of the editing transcripts through tRNA-like sequences (TLS) and the cisgenic-like approach to remove transgenes.

Intra-block variations of vine water status in time and space

Vine water status was measured on 96 plots of three vines inside a vineyard block of 0.28 ha during three years: 2003, 2004 and 2005. Three physiological indicators were implemented: stem water potential, carbon isotope discrimination measured on grape sugars at ripeness (δ13C) and canopy temperature measured by high resolution remote sensing. For stem water potential, measurements were taken on every single vine of each plot.