Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of nitrogen content on fermentation kinetics and aroma profile of assyrtiko wine

Effect of nitrogen content on fermentation kinetics and aroma profile of assyrtiko wine

Abstract

Today, there is need to design, produce and label terroir wines, with unique organoleptic properties and more “attractive to consumers”. For this purpose, two Saccharomyces cerevisiae yeast strains (Sa and Sb) isolated during spontaneous fermentations were used for white wine production from the Assyrtiko grape of Santorini. A third commercial strain was used as control. Two concentrations of Yeast Assimilable Nitrogen (YAN) and DAP (diammonium phosphate) were added to the must (150mg/L and 250mg/L) in order to evaluate the effcet of nitrogen content on the final wine quality. Both analytical chemical methods (HPLC, GC-MS, classical eonological methods) and sensory analysis were employed to assess the chemical composition of the wines and their organoleptic character. In addition fermentation kinetics were monitored throughout the experiment. By the second day of fermentation all three strains had consumed approximately 75% of amino acids. Differences among strains were observed concerning inorganic nitrogen requirements. Sa strain consumed it faster and was the first to compete fermentation independently from the level of added YAN. The commercial strain was characterized by the highest concentration of residual sugars, followed by Sb and Sa. Alcohol content ranged from 12.8-13.1% vol. Sb and the commercial one produced significant higher amounts of glycerol (about 0.7g/L), especially in the case of lower YAN. Sb also produced significant higher amounts of higher alcohols (1.9-fold) and ketones (5.6-fold) but significant lower amounts of esters (1.2-fold) in comparison with the commercial strain. Sa was characterized by significant higher concentrations of fatty acids (2.1-fold) and lower acetic acid (1.6-fold) production. No statistically important differences were observed in the oligomeric phenolic compound content of the samples. Both indigenous strains scored better results in overall aroma quality, and more specifically in “fruity”, “floral” descriptors compared with control. They were also preferred over the commercial strain as far as mouthfeel, body and acidity are concerned. The evaluation of both chemical and sensory data indicated the potential of the indeginous starins for commercial wine production with unique characteristics and high quality.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Stefania Christofi, M.Dimopoulou1 Α, Papanikolaou1 G.J, M.Sadoveanu Alley

1 Department of Food Science & Human Nutrition, Laboratory of Oenology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece, Terpou1 S, Nychas1  C.I., Bogdan2 Romania Academy – Iasi Branch, Research Centre for Oenology, Iasi 700490, Romania V., Cotea3 University of Agricultural Sciences and Veterinary Medicine Iaşi, 3 M. Sadoveanu Alley, Iaşi, 700490, Romania Kallithraka, S1.

Contact the author

Keywords

aminoacids, fermentation kinetics, saccharomyces cerevisiae, volatile compounds

Citation

Related articles…

“Compost Application in the Vineyard: Effects on Soil Nutrition and Compaction”

The mechanization of pruning and harvesting in vineyards has increased the risk of soil compaction. To reclaim soil properties or avoid this degradation process, it is crucial to properly manage the soil organic matter, and the application of compost derived from the vines themselves is a strategy to achieve this. The objective of this study was to evaluate the properties of soil treated with different doses of compost applied both on the vine row and the inter rows of a Vitis vinifera crop.

Implementing VIS-NIR spectroscopy as a rapid and non-intrusive technique for assessing anthocyanin and phenolic concentrations in Vitis vinifera L. Grenache whole grape berries

Anthocyanins and phenolic compounds play a crucial role in winemaking, contributing to the profile, flavor, color, texture, and stability of wine. Grape clusters, specifically Vitis vinifera L. cv. Grenache, were handpicked from a commercial vineyard sited in Tudelilla, La Rioja, Spain (42°18′ 52.26″, Long. -2°7′ 59.15″, Alt. 582 m) on five distinct dates from veraison to harvest during the 2015 season. Non-contact spectral measurements were conducted on intact grape berries using a VIS-NIR spectrometer operating in the 570 – 1000 nm spectral range under controlled laboratory conditions, positioned at a distance of 25 cm from the berries. The quantification of 16 anthocyanins and phenols in 120 grape clusters was performed using HPLC, established as the reference method for validating the spectral tool.

Grape berry size is a key factor in determining New Zealand Pinot noir wine composition

Making high quality but affordable Pinot noir (PN) wine is challenging in most terroirs and New Zealand’s (NZ) situation is no exception. To increase the probability of making highly typical PN wines producers choose to grow grapes in cool climates on lower fertility soils while adopting labour intensive practices. Stringent yield targets and higher input costs necessarily mean that PN wine cost is high, and profitability lower, in line-priced varietal wine ranges. To understand the reasons why higher yielding vines are perceived to produce wines of lower quality we have undertaken an extensive study of PN in NZ. Since 2018, we established a network of twelve trial sites in three NZ regions to find individual vines that produced acceptable commercial yields (above 2.5kg per vine) and wines of composition comparable to “Icon” labels. Approximately 20% of 660 grape lots (N = 135) were selected from within a narrow juice Total Soluble Solids (TSS) range and made into single vine wines under controlled conditions. Principal Component Analysis of the vine, berry, juice and wine parameters from three vintages found grape berry mass to be most effective clustering variable. As berry mass category decreased there was a systematic increase in the probability of higher berry red colour and total phenolics with a parallel increase in wine phenolics, changed aroma fraction and decreased juice amino acids. The influence of berry size on wine composition would appear stronger than the individual effects of vintage, region, vineyard or vine yield. Our observations support the hypothesis that it is possible to produce PN wines that fall within an “Icon” benchmark composition range at yields above 2.5kg per vine provided that the Leaf Area:Fruit Weight ratio is above 12cm2 per g, mean berry mass is below 1.2g and juice TSS is above 22°Brix.

Effect of spray with autochthonous Trichoderma strains and its secondary metabolites on the quality of Tempranillo grape

Trichoderma is one of the most widely used fungal biocontrol agents on vineyards due to its multiple benefits on this crop, such as its fungicidal and growth promoting capacity. In this work, we have analyzed the effect on the concentration of nutrients in grapevine leaves and on the quality of the grape must after spraying an autochthonous strain of Trichoderma harzianum and one of the main secondary metabolites produced by this genus, 6-pentyl-α-pyrone (6PP).

Ten years soil diagnosis in vineyards, with particularly analysis of organic and microbial mass and measuring their evolution

Since 1996, we study the soil in viticulture, specially in the South of France. In the field, we delimit soil units and observe soil profiles and take samples to analyse its physical, mineral, organic and microbial mass composition