Macrowine 2021
IVES 9 IVES Conference Series 9 Expanding the biotechnological potential of M. pulcherrima/fructicola clade for wine-related applications

Expanding the biotechnological potential of M. pulcherrima/fructicola clade for wine-related applications

Abstract

AIM: Strains belonging to M. pulcherrima/fructicola clade are frequently isolated from flowers, fruits and grape musts, and exhibit a broad spectrum of enzymatic activities and antimicrobial potential (Morata et al., 2019; Sipiczki, 2020; Vicente et al. 2020). By reason of these features, selected strains of this clade have been proposed as non-Saccharomyces starter cultures for winemaking. In this study, with a view to valorise the biotechnological potential of these strains, a new Metschnikowia sp. strain, DBT012, was selected for application in vinification trials of Valpolicella’s fresh and withered typical grapes for reduction of SO2addition and increase of aromatic complexity. Further, in the framework of the investigation on the biodiversity of a collection of strains, distinct pulcherrimin-producing isolates from spontaneous fermentation and grapes were putatively recognized as Metschnikowia spp. and selected for genotypic and phenotypic characterisation.

METHODS: Lyophilised cultures of the reference strain DBT012 were tested in pilot-scale vinifications (mixed-cultures with S. cerevisiae). The isolates were characterised based on enzymatic activities (e.g., sulphite-reductase and beta-glucosidase activities) and growth under different wine-related stress conditions (e.g., ethanol, high sugar content). Genetic fingerprinting techniques (e.g., (GTG)5 and microsatellite) were performed for de-replication of isolates and strain typing. Whole-genome sequencing of the reference strain was carried out to analyse the correlation between genotype and phenotype, and comparative analyses with available type strains were performed to deepen the taxonomic aspects and molecularly identify this strain.

RESULTS: Use of Metschnikowia sp. DBT012 reduced the acetaldehyde content in wines, and positively influenced the wine aroma. About 50 Metschnikowia strains were characterised, which displayed a significant phenotypic diversity in terms of β-glucosidase and esterase activities, H2S production and growth at high sugar concentrations. The relationship of those features with genotypic and genomic distinctiveness of the reference strain was highlighted.

CONCLUSIONS:

The well-characterised thematic collection of strains belonging to M. pulcherrima/fructicola clade presented here constitutes an important reservoir of biodiversity for applications in different wine-related scenarios.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Eleonora Troiano,Renato, LEAL BINATI, Ilaria, CHECCHIA, Ilaria, LARINI, Veronica, GATTO, Gianluca, VENERI, Giacomo, ZAPPAROLI, Vittorio, CAPOZZI, Elisa, SALVETTI, Sandra, TORRIANI, Giovanna, E., FELIS

Department of Biotechnology, University of Verona, Italy, Department of Biotechnology, University of Verona, Italy, Department of Biotechnology, University of Verona, Italy, Department of Biotechnology, University of Verona, Italy, Department of Biotechnology, University of Verona, Italy, Department of Biotechnology, University of Verona, Italy , Department of Biotechnology, University of Verona, Italy, Institute of Sciences of Food Production, National Research Council (CNR), Italy, Department of Biotechnology, University of Verona, Italy, Department of Biotechnology, University of Verona, Italy, Department of Biotechnology, University of Verona, Italy

Contact the author

Keywords

metschnikowia, wine microbiology, biodiversity, biotechnological potential

Citation

Related articles…

Updating the Winkler index: An analysis of Cabernet sauvignon in Napa Valley’s varied and changing climate

This study aims to create an updated, agile viticultural climate index (similar to the Winkler Index) by performing in-depth analyses of current and historical data from industry partners in several major winegrowing regions. The Winkler Index was developed in the early twentieth century based on analysis of various grape-growing regions in California. The index uses heat accumulation (i.e. Growing Degree Days) throughout the growing season to determine which grape varieties are best suited to each region. As viticultural regions are increasingly subject to the complexity and uncertainty of a changing climate, a more rigorous, agile model is needed to aid grape growers in determining which cultivars to plant where. For the first phase of this study, 21 industry partners throughout Napa Valley shared historical phenology, harvest, viticultural practice, and weather data related to their Cabernet sauvignon vineyard blocks. To complement this data, berry samples were collected throughout the 2021 growing season from 50 vineyard blocks located throughout 16 American Viticultural Areas that were then analyzed for basic berry chemistry and phenolics. These blocks have been mapped using a Geographic Information System (GIS), enabling analysis of altitude, vineyard row orientation, slope, and remotely sensed climate data. Sampling sites were also chosen based on their proximity to a weather station. By analyzing historical data from industry partners and data specifically collected for this study, it is possible to identify key parameters for further analysis. Initial results indicate extreme variability at a high spatial resolution not currently accounted for in modern viticultural climate indices and suggest that viticultural practices play a major role. Using the structure of data collection and analyses developed for the first phase, this project will soon be expanded to other wine regions globally, while continuing data collection in Napa Valley.

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).

How can historical cultivars mitigate the effects of climate change?

IFV, INRAe and the national network “Partenaires de la Sélection Vigne” representing 37 organizations from the different wine regions, have been working increasingly closely over the last 2 decades towards the preservation of the French varietal patrimony. There are approximately 600 patrimonial varieties according to INRAe and SupAgro Montpellier experts, including ancient cultivars (400) and intravarietal crossbreeds obtained since the 19th century. In the context of a drastic reduction in such varieties from the mid 1980’s in favor of mainstream varieties, it was essential to carry out an inventory of old vines and vineyards. INRAe Vassal collection plays a key role here as it holds the largest diversity available, along with a rich bibliography and herbariums, offering us the opportunity to document and double check the identity of a cultivar, consolidating the expertise of ampelographers. The work is carried out in several stages, from verifying the existence of a variety in a small region, through to rehabilitation. During this session, the authors present the process that leads to the official registration of a variety. After this, IFV selection center takes over to initiate the process of selection and propagation. A specific focus within regions such as the Alps, Champagne and the South-West will provide details of the full procedure. Bia, Bouysselet, Chardonnay rose, Mecle and the aptly named Tardif, are some of the cultivars that have followed this procedure. Furthermore, a recent regulation established by INAO on “varieties of interest for adaptation purposes” might boost uptake by growers. Since 2006, 36 historical cultivars have been registered. Most of these have been neglected in the past due to late maturity, lack of sugar and high titratable acidity at harvest time. Such characteristics are today considered as positive qualities, not only in mitigation of the effects of climate change, but also as an opportunity for restoring diversity…

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.