Macrowine 2021
IVES 9 IVES Conference Series 9 Screening of soil yeasts with fermentative capacity from the antarctic continent for their application in the wine industry

Screening of soil yeasts with fermentative capacity from the antarctic continent for their application in the wine industry

Abstract

AIM: In the last years, many wineries are increasing experimentation to produce more distinguishable beverages. In this sense, the reduction of the fermentation temperature could be a useful tool because it preserves volatile compounds and prevents wines from browning, particularly in the case of white wines. However, low fermentation temperatures usually produce an early stop in the fermentation. Hence, the employment of new yeast strains able to operate at low temperatures could solve this problem, giving rise to different characteristics in wines. The Antarctic Continent is a crucial source for the isolation of new microorganisms and bioactive metabolites, given the competitive conditions of this environment with low temperatures, scarce carbon sources and high UV radiation. Considering this, the objective of this study was the isolation and characterization of fermentative yeast from the Antarctic Continent with potential for use in the wine industry.

METHODOLOGY: Six soil samples collected in Fildes Bay, west of King George Island and three soil samples from King George Island were processed for yeast isolation. Samples were suspended in sterile water and dilutions of each suspension were inoculated onto yeast medium (YM) agar plates with antibiotics, which were then incubated at 4, 10, and 18 °C until visible colony growth. Colonies with a non-filamentous appearance were selected, which were reseeded on YM agar without antibiotics. Alcohol tolerance was performed using concentrations of 3, 6, or 9% alcohol. Later on, sugar tolerance was analyzed using fructose and glucose in a 1:1 proportion; with 5, 10, 15, 20, or 25% of sugar in the medium. Those isolates with microscopic characteristics of interest were selected to determine fermentative activity in vitro using a simple colorimetric assay with phenol red, as a pH indicator. To differentiate the isolates, and discard replicates, a fingerprinting assay with arbitrary primers was performed. Identification of the isolates was carried out using PCR and ITS region primers with BLAST bioinformatics tools.

RESULTS: Nine soil samples collected from the Antarctica were processed for yeast isolation. We obtained 125 yeasts from the soil samples, with a growth temperature of 10ºC. Overall, 25 yeasts have fermentative activity and are able to tolerate a culture medium with at least 20% glucose and up to 6% of ethanol. The isolates were also characterized by optical microscopy and fingerprinting using PCR with arbitrary primers to discard identical strains and allowed us to discriminate 10 unique strains with fermentative capacity from the 25 isolates. To determine the identity of the isolated yeasts, the amplification and sequence of the 18S RNA was performed.

CONCLUSION:

The Antarctic continent has proven to be a source of fermentative yeasts with high potential for their use in the wine industry.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Laura Navarro, Cristina Úbeda, Mariona Gil i Cortiella, Ana Gutierrez, Gino Corsini, Nancy Calisto

Biomedical Sciences Institute, Health Sciences Faculty, Autonomous University of Chile, Santiago, Chile., Nutrition and Food Sciences Department, Faculty of Pharmacy, University of Seville, Seville, Spain. Applied Chemical Sciences Institute, Autonomous University of Chile, Santiago, Chile. Biomedical Sciences Institute, Health Sciences Faculty, Autonomous University of Chile, Santiago, Chile. Biomedical Sciences Institute, Health Sciences Faculty, Autonomous University of Chile, Santiago, Chile. Chemical engineering department, Faculty of Engineering, Magallanes University, Punta Arenas, Chile. Biomedical Sciences Institute, Health Sciences Faculty, Autonomous University of Chile, Santiago, Chile.

Contact the author

Keywords

antarctic yeast, low temperature fermentation, yeast isolation, yeast characterization

Citation

Related articles…

Climate change impacts on Douro Region viticulture and adaptation measures

Climate has a significant impact in the success of any agricultural system, with a direct influence on the crops suitability to a given region, interfering on yield and quality and also with the economic sustainability of the productive activity. In the Douro Demarcated Region (RDD), as in most regions of the Mediterranean climate, the scarce precipitation (33% has less than 600 mm per year), and your high variability, associated with high rates of evapotranspiration during the summer, is usually one of the fundamental factors that limit the grapevine development, as well as the production and quality of the harvest. Thus, facing the scenario in temperature changes for the next decades (1.5-2.5°C) and confirming the predictions of precipitation decreases and/or great variability in the occurrence of heat waves and intense rainfall, the consequences for slope stability in mountain viticulture and sustainability of all operations involved, are risks to be taken into account. In this way, a deepest and sustained knowledge regarding the adaptation measures to adverse environmental conditions is of a crucial importance, enabling a more efficient adaptation of plant growth conditions and the optimization of production and quality of the grapevines. The development of this work, carried out in two commercial vineyards, one located in Soutelo do Douro, São João da Pesqueira, Cima Corgo sub-region, and another located in Numão, Vila Nova de Foz Côa, Douro Superior sub-region, it seeks to establish a relationship between climatic elements and physiological, productive and qualitative parameters, as well as to evaluate the effectiveness of adaptation measures, including different types of deficit irrigation (2002-2019) and the application of shading nets (2019-2020) in the physiological, viticultural and oenological behavior in the Touriga Nacional and Moscatel Galego Branco varieties, respectively. The results showed that the application of deficit irrigation allowed to significantly reduce the impact of the adverse weather conditions at key moments in the development of the grapevine, particularly in the period immediately before veráison and maturation, reducing the negative effects on the physiological processes and productivity, without compromise the must quality parameters. On the other hand, the application of shading nets significantly reduced de leaves temperature, allowing to increase the water potential, stomatal conductance and photosynthetic rate of grapes, which was reflected in the yield increase in the 2nd year of the study. For the maturation indicators, higher levels of total acidity, malic acid and assimilable nitrogen were obtained. The last measure presents a huge potential, being essential to carry out more years of trials to obtain stronger conclusions in terms of production parameters, but also in characteristics as important as the grape ripening components and the organoleptic characteristics of wines.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

Heatwaves and grapevine yield in the Douro region, crop model simulations

Heatwaves or extreme heat events can be particularly harmful to agriculture. Grapevines grown in the Douro winemaking region are particularly exposed to this threat, due to the specificities of the already warm and dry climatic conditions. Furthermore, climate change simulations point to an increase in the frequency of occurrence of these extreme heat events, therefore posing a major challenge to winegrowers in the Mediterranean type climates. The current study focuses on the application of the STICS crop model to assess the potential impacts of heatwaves in grapevine yields over the Douro valley winemaking region. For this purpose, STICS was applied to grapevines using high-resolution weather, soil and terrain datasets over the Douro. To assess the impact of heatwaves, the weather dataset (1989-2005) was artificially modified, generating periods with anomalously high temperatures (+5 ºC), at certain onset dates and with specific durations (from 5 to 9 days). The model was run with this modified weather dataset and results were compared to the original unmodified runs. The results show that heatwaves can have a very strong impact on grapevine yields, strongly depending on the onset dates and duration of the heatwaves. The highest negative impacts may result in a decrease in the yield by up to -35% in some regions. Despite some uncertainties inherent to the current modelling assessment, the present study highlights the negative impacts of heatwaves on viticultural yields in the Douro region, which is critical information for stakeholders within the winemaking sector for planning suitable adaptation measures.

Copper contamination in vineyard soils of Bordeaux: spatial risk assessment for the replanting of vines and crops

Copper (Cu) is widely and historically used in viticulture as a fungicide against mildew. Cu has a strong affinity for soil organic matter and accumulates in topsoil horizons. Thus, Cu may negatively affect soil organisms and plants, consequently reducing soil fertility and productivity. The Bordeaux vineyards have the largest vineyard surfaces (26%) within French controlled appellation and a great proportion of French wine production (around 5 million hl per year). Considering the local context of vineyard surfaces decreasing (vine uprooting) and possible new crop plantation, the issue of Cu potential toxicity rises. Therefore, the aims of this work are firstly to evaluate the Cu contamination in vineyard soils of Bordeaux, secondly to produce a risk assessment map for new vine or crop plantation. We used soil analyses from several local studies to build a database with 4496 soil horizon samples. The database was enhanced by means of pedotransfer functions in order to estimate the bioaccessible (EDTA-extractable) Cu in soils of samples without measurements. From this database, 1797 georeferenced samples with CuEDTA concentrations in the topsoil (0-50 cm depth) were used for kriging interpolation in order to produce the spatial distribution map of CuEDTA in vineyard soils. Then, the spatial distribution of Cu was crossed with vine uprooting surfaces and municipality boundaries. CuEDTAconcentrations ranged from 0.52 to 459 mg/kg and showed clear anomalies. Our results from spatial analysis showed that almost 50% of vineyard soil surfaces have CuEDTA concentrations higher than 30 mg/kg (moderate risk for new plantation) and 20% with concentrations higher than 50 mg/kg (high risk for new plantation). A decision-support map based on municipalities was realised to provide a simple tool to stakeholders concerned by land use management.

Climate modeling at local scale in the Waipara winegrowing region in the climate change context

In viticulture, a warming climate can have a very significant impact on grapevine development and therefore on the quality and characteristics of wines across different spatial scales, ranging from global to local. In order to adapt wine-growing to climate change, global climate models can be used to define future scenarios, but only at the scale of major wine regions. Despite the huge progress made over the last ten years in terms of the spatial resolution of climate models (now downscaled to a few square kilometres), they are not yet sufficiently precise to account for the local climate variability associated with such parameters as local topography, in spite of these parameters being decisive for vine and wine characteristics. This study describes a method to downscale future climate scenarios to vineyard scale. Networks of data loggers have been used to collect air temperature at canopy level in the Waipara winegrowing region (New Zealand) over five growing seasons. These measurements allow the creation of fine-scale geostatistical models and maps of temperature (at 100 m resolution) for the growing season. In order to model climate change at pilot site scale, these geostatistical models have been combined with regional climate change predictions for the periods 2031-2050 and 2081-2100 based on the RCP8.5 climate change scenario. The integration of local climate variability with regionalized climate change simulations allows assessment of the impacts of climate change at the vineyard scale. The improved knowledge gained using this methodology results from the increased horizontal resolution that better addresses the concerns of winegrowers. The results provide the local winegrowers with information necessary to understand current processes, as well as historical and future viticulture trends at the scale of their site, thereby facilitating decisions about future response strategies.