Macrowine 2021
IVES 9 IVES Conference Series 9 Screening of soil yeasts with fermentative capacity from the antarctic continent for their application in the wine industry

Screening of soil yeasts with fermentative capacity from the antarctic continent for their application in the wine industry

Abstract

AIM: In the last years, many wineries are increasing experimentation to produce more distinguishable beverages. In this sense, the reduction of the fermentation temperature could be a useful tool because it preserves volatile compounds and prevents wines from browning, particularly in the case of white wines. However, low fermentation temperatures usually produce an early stop in the fermentation. Hence, the employment of new yeast strains able to operate at low temperatures could solve this problem, giving rise to different characteristics in wines. The Antarctic Continent is a crucial source for the isolation of new microorganisms and bioactive metabolites, given the competitive conditions of this environment with low temperatures, scarce carbon sources and high UV radiation. Considering this, the objective of this study was the isolation and characterization of fermentative yeast from the Antarctic Continent with potential for use in the wine industry.

METHODOLOGY: Six soil samples collected in Fildes Bay, west of King George Island and three soil samples from King George Island were processed for yeast isolation. Samples were suspended in sterile water and dilutions of each suspension were inoculated onto yeast medium (YM) agar plates with antibiotics, which were then incubated at 4, 10, and 18 °C until visible colony growth. Colonies with a non-filamentous appearance were selected, which were reseeded on YM agar without antibiotics. Alcohol tolerance was performed using concentrations of 3, 6, or 9% alcohol. Later on, sugar tolerance was analyzed using fructose and glucose in a 1:1 proportion; with 5, 10, 15, 20, or 25% of sugar in the medium. Those isolates with microscopic characteristics of interest were selected to determine fermentative activity in vitro using a simple colorimetric assay with phenol red, as a pH indicator. To differentiate the isolates, and discard replicates, a fingerprinting assay with arbitrary primers was performed. Identification of the isolates was carried out using PCR and ITS region primers with BLAST bioinformatics tools.

RESULTS: Nine soil samples collected from the Antarctica were processed for yeast isolation. We obtained 125 yeasts from the soil samples, with a growth temperature of 10ºC. Overall, 25 yeasts have fermentative activity and are able to tolerate a culture medium with at least 20% glucose and up to 6% of ethanol. The isolates were also characterized by optical microscopy and fingerprinting using PCR with arbitrary primers to discard identical strains and allowed us to discriminate 10 unique strains with fermentative capacity from the 25 isolates. To determine the identity of the isolated yeasts, the amplification and sequence of the 18S RNA was performed.

CONCLUSION:

The Antarctic continent has proven to be a source of fermentative yeasts with high potential for their use in the wine industry.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Laura Navarro, Cristina Úbeda, Mariona Gil i Cortiella, Ana Gutierrez, Gino Corsini, Nancy Calisto

Biomedical Sciences Institute, Health Sciences Faculty, Autonomous University of Chile, Santiago, Chile., Nutrition and Food Sciences Department, Faculty of Pharmacy, University of Seville, Seville, Spain. Applied Chemical Sciences Institute, Autonomous University of Chile, Santiago, Chile. Biomedical Sciences Institute, Health Sciences Faculty, Autonomous University of Chile, Santiago, Chile. Biomedical Sciences Institute, Health Sciences Faculty, Autonomous University of Chile, Santiago, Chile. Chemical engineering department, Faculty of Engineering, Magallanes University, Punta Arenas, Chile. Biomedical Sciences Institute, Health Sciences Faculty, Autonomous University of Chile, Santiago, Chile.

Contact the author

Keywords

antarctic yeast, low temperature fermentation, yeast isolation, yeast characterization

Citation

Related articles…

An analytical framework to site-specifically study climate influence on grapevine involving the functional and Bayesian exploration of farm data time series synchronized using an eGDD thermal index

Climate influence on grapevine physiology is prevalent and this influence is only expected to increase with climate change. Although governed by a general determinism, climate influence on grapevine physiology may present variations according to the terroir. In addition, these site-specific differences are likely to be enhanced when climate influence is studied using farm data. Indeed, farm data integrate additional sources of variation such as a varying representativity of the conditions actually experienced in the field. Nevertheless, there is a real challenge in valuing farm data to enable grape growers to understand their own terroir and consequently adapt their practices to the local conditions. In such a context, this article proposes a framework to site-specifically study climate influence on grapevine physiology using farm data. It focuses on improving the analysis of time series of weather data. The analytical framework includes the synchronization of time series using site-specific thermal indices computed with an original method called Extended Growing Degree Days (eGDD). Synchronized time series are then analyzed using a Bayesian functional Linear regression with Sparse Steps functions (BLiSS) in order to detect site-specific periods of strong climate influence on yield development. The article focuses on temperature and rain influence on grape yield development as a case study. It uses data from three commercial vineyards respectively situated in the Bordeaux region (France), California (USA) and Israel. For all vineyards, common periods of climate influence on yield development were found. They corresponded to already known periods, for example around veraison of the year before harvest. However, the periods differed in their precise timing (e.g. before, around or after veraison), duration and correlation direction with yield. Other periods were found for only one or two vineyards and/or were not referred to in literature, for example during the winter before harvest.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Extreme canopy management for vineyard adaptation to climate change: is it a good idea?

Climate change constitutes an enormous challenge for humankind and for all human activities, viticulture not being an exception. Long-term strategic changes are probably needed the most, but growers also need to deal with short-term changes: summers that are getting progressively warmer, earlier harvest dates and higher pH in musts and wines. In the last 10-15 years, a relevant corpus of research is being developed worldwide in order to evaluate to which extent extreme canopy management operations, aimed at reducing leaf area and, thus, limiting the source to sink ratio, could be useful to delay ripening. Although extreme canopy management can result in relevant delays in harvest dates, longer term studies, as well as detailed analysis of their implications on carbohydrate reserves, bud fertility and future yield are desirable before these practices can be recommended.

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.

Postveraison shoot trimming in Tannat and Merlot: preliminary results on yield components, plant balance and berry composition

There is currently a trend towards the production of wines with low alcohol content. To achieve this, grapes with low sugar content must be used. There are techniques at the vineyard level that can delay ripening and avoid excessive sugar accumulation without, a priori, affecting the final polyphenol content. Postveraison shoot trimming (PVST) is experimentally evaluated for these purposes, but its impact under Uruguayan climatic conditions with high interannual variability is not known. The aim of this work is to assess the PVST in Tannat and Merlot cultivars and their impact on yield components, plant balance and berry primary composition. In this study, two commercial vineyards of 10 years old Tannat and Merlot (grafted on SO4) at Canelones Department were selected. During the 2020-201 growing season, grapevines were submitted to PVST when grapes reached 15º Brix. In a randomized block, trimmed (T) and control (C) plants were evaluated with three repetitions each cultivar. Evaluation of the evolution of primary berry composition during ripening, measurement of yield components and plant balance were performed. For both cultivars, PVST did not affect yield components. Merlot reached 5.4 kg per plant and Tannat 7.1 kg, with not statistical significance between treatments. However, statistical differences were observed in terms of plant balance. In Merlot Ravaz Index reached a difference of 5.3 (12.0 in T and 6.7 in C) meanwhile Tannat reached 3.5 of statistical difference (13.7 in T and 10.2 in C). The tendency to imbalance for the treated plants had an impact on the final grape composition. Merlot grapes showed statistical difference in final total acidity (0.3 g of difference between treatments) while treatments impact final sugar content on Tannat grapes (10.0 g of difference between treatments). Further studies are needed to assess the impact of different canopy management techniques in our conditions.