Macrowine 2021
IVES 9 IVES Conference Series 9 Characterization of Brettanomyces bruxellensis biofilm, a resistance strategy to persist in wine-related environments

Characterization of Brettanomyces bruxellensis biofilm, a resistance strategy to persist in wine-related environments

Abstract

AIM: Biofilm is a resistance mechanism deployed by microorganisms to adapt to stresses, leading to their persistence in the environment. In the case of Brettanomyces bruxellensis, a wine spoilage yeast, knowledge about its capacity to form biofilm remains limited although this potential strategy could explain its recurring presence in cellars. In this study, we propose to investigate the capacity of strains of B. bruxellensis to form biofilm according to different conditions and to characterize its structure.

METHODS: Sixty-five isolates of B. bruxellensis were sampled from a cellar and discriminated into genetic groups by microsatellite analysis. The capacity of selected strains to adhere and form biofilm has been investigated in different conditions of media and supports, related to wine environment. The structure of the biofilm and its components were explored using several microscopic techniques as confocal laser scanning microscopy, electronic microscopy and epifluorescence microscopy.

RESULTS: Twelve strains among the 65 isolates were selected and have showed the capacity to form biofilm on polystyrene surface. Microscopic observations of the biofilm revealed microcolonies, filamentous cells and extracellular polymeric substances despite a small thickness. Then, the study of the impact of wine on B. bruxellensis biofilm revealed biofilm cell released and growth of these released biofilm cells, probable contamination source of the wine. Finally, for both planktonic and biofilm lifestyles in wine, a new chlamydospore-like element was observed for B. bruxellensis, described as a resistance form in other fungi.

CONCLUSIONS: This study validates the capacity of B. bruxellensis to form biofilm and highlights structural element of this mode of life. Moreover, an additional resistance strategy was suggested through the description of the chlamydospore-like elements. New insights into the persistence of B. bruxellensis during the winemaking process and in wineries have emerged.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Manon Lebleux, Hany ABDO, Louise BASMACIYAN, Julie LAURENT, Chloé ROULLIER-GALL, Hervé ALEXANDRE, Michèle GUILLOUX-BENATIER, Stéphanie WEIDMANN, Sandrine ROUSSEAUX

Univ. Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, VAlMiS Laboratory-IUVV, Dijon, France

Contact the author

Keywords

Brettanomyces bruxellensis, biofilm, wine spoilage

Citation

Related articles…

Foliar application of specific inactivated yeast to enhance the varietal aroma precursors accumulation on cv. Traminer

The production of grapes with a balanced composition is one of the main goals that agronomists and oenologists pursue to produce premium quality wines.

Barrel-to-Barrel Variation of Color and Phenolic Composition in Barrel-Aged Red Wine

Tangible variation of sensory characteristics is often perceived in wine aged in similar barrels. This variation is mostly explained by differences in the wood chemical composition, and in the production of the barrels. Despite these facts, the literature concerning barrel-to-barrel variation and its effect on wine sensory and chemical characteristics is very scarce [1]. In this study, the barrel-to-barrel variation in barrel-aged wines was examined in respect of the most important phenolic compounds of oenological interest and chromatic characteristics, considering both the effects of the (individual) barrel and cooperage. A red wine was aged in 49 new medium-toasted oak (Quercus petraea) barrels, from four cooperages, for 12 months

The relationship between wind exposure and viticultural performance of Vitis vinifera L. cv. Merlot in a coastal vineyard (South Africa)

The South Western Cape of South Africa is exposed to strong southerly and south easterly synoptic winds during the growth period of the grapevine. The development of sea breezes in the afternoon is also a phenomenon associated with the ripening period of grapes cultivated in this coastal area. Wind is one of the environmental variables having the greatest spatial variation but the implications of regular exposure to wind for the performance of the grapevine has not yet been determined for vineyards in the South Western Cape. This study was initiated to meet this need.
The study was conducted in a hedge-trellised vineyard of Vitis vinifera L. cv Merlot with north east – south west row direction. Thirty experimental sites, each consisting of 14 vines, were identified as being exposed to wind or sheltered based on hand-held anemometer readings during the 2001/2002 season. Four stationary anemometers were strategically positioned between the thirty sites. Stomatal conductance and leaf temperature were measured with a PP systems porometer. Vegetative and yield measurements were performed during the 2002/2003 season. The t-test of equal variance was used to determine significant differences in measured parameters between exposed and sheltered grapevines.
Stomatal conductance and leaf area were significantly reduced by exposure to wind. This was associated with a significant reduction in the leaf area of primary shoots, related to shorter shoots, but a significant augmentation of secondary shoot leaf number and area. The number of bunches per vine and yield were also reduced for exposed vines. The berry potassium content was significantly increased for exposed grapevines.
This demonstrates that exposure to wind can result in significant within-vineyard, and potentially between-vineyard, variability in grapevine physiology, vegetative growth, yield and berry composition, with implications for wine style and quality.

Extraction of stilbenes from grape cane waste and their possible applications

Vine pruning residues constitute a significant fraction of vitivinicultural waste; in fact, depending on the variety and training system, they can reach 1-5 tons/ha/year.

Sensory profiles and European Consumer Preference related to Aroma and Phenolic Composition of Wines made from Fungus Resistant Grape Varieties

New grape varieties with several resistance loci towards powdery and downy mildew allows to significantly reduce the use of fungicides