Application of plant growth regulators on Vitis vinifera L var. Mouchtaro affect berry quality characteristics & associated microbial communities

Hydroxytyrosol (HT) is a phenolic compound present in olives, virgin olive oil and wine. HT has attracted great scientific interest due to its biological activities which have been related with the ortho-dihydroxy conformation in the aromatic ring. In white and red wines, HT has been detected at concentrations ranging from 0.28 to 9.6 mg/L and its occurrence has been closely related with yeast metabolism of aromatic amino acids by Ehrlich pathway during alcoholic fermentation. One of the most promising properties of this compound is the neuroprotective activity against pathological mechanisms related with neurode-generative disorders including Alzheimer’s and Parkinson’s disease.

Tempranillo Tinto (TT) is the third-most planted red wine variety in the world, and it is mostly grown in the Iberian Peninsula. Spontaneous somatic variation appearing during vegetative propagation can be exploited to improve elite varieties as Tempranillo Tinto, including the selection of new phenotypes enhancing berry quality. We described previously that a somatic variant of TT with darker fruit color, the clone VN21, exhibits increased extractability of polyphenols during the winemaking process. To unravel the molecular mechanism underlying this phenomenon, we performed whole-genome resequencing to compare VN21 to other TT clones, revealing a 10 Mb deletion in chromosome 11 that likely affected only the L1 meristem cell layer of VN21 and tissues derived from it, such as external cell layers of berry skin.

Vine water use is a critical determinant of vineyard management practices, especially in the context of climate change.

The current levels of greenhouse gas emissions are expecting to provoke a change on the environmental conditions which, among others, will include a rise of global mean surface temperature and an increment of atmospheric CO2 levels (IPCC, 2014), known as climate change. The response of grapevine (Vitis vinifera L.), one of the most important crops in Europe, from both a cultural and economic point of view, is not completely understood yet and the studies considering the interaction between factors are scarce. Besides, the potential variety of responses among somatic variants needs to be studied in order to be exploited in the avoidance of undesired traits linked to climate change (Carbonell‐Bejerano et al., 2015).

High power ultrasound has been recently recognized one of the most promising technologies in winemaking processes, especially after the recent OIV resolution, concerning the application of ultrasounds on crushed grapes to promote the extraction of skin compounds.