Wine microbial diversity and cross-over applications: emerging results and future perspectives

Grapevine downy mildew (GDM), caused by the oomycete Plasmopara viticola, is one of the most destructive diseases of Vitis vinifera worldwide. All V. vinifera cultivars are susceptible to P. viticola infection, and epidemics can spread across an entire vineyard within a matter of weeks. Severe outbreaks cause substantial reductions in yield and fruit quality. Tracking GDM spread by manual scouting is time-consuming and unfeasible over large spatial extents.

Aims: The aims of this study are to determine the influences of the local geology, mineralogy and geochemistry of surroundings, substrate and soil on the cultivation of vines, these as an additional factor of specificity and locality in the production of wine and definition of terroir, as well as for the discrimination of local variance of substrate and soil properties for the strategic management of cultivation plots and/or the evaluation of new cultivation regions, necessary within a scope of global climate change.

The concept of viticultural terroir includes soil, sub-soil, and climatic factors but also many management viticultural and oenological practices which are chosen according to know-how of the winegrowers.

Measurement of carbon isotope discrimination in berry juice sugars at maturity (δ13C) provides an integrated assessment of water use efficiency (WUE) during the period of berry ripening, and when collected over multiple seasons can be used as an indication of drought stress response. Berry juice δ13C measurements were carried out on 48 different varieties planted in a common garden experiment in Bordeaux, France from 2014 through 2021 and were paired with midday and predawn leaf water potential measurements on the same vines in a subset of six varieties. The aim was to discriminate a large panel of varieties based on their stomatal behaviour and potentially identify hydraulic traits characterizing drought tolerance by comparing δ13C and hydroscapes (the visualisation of plant stomatal behaviour as a response to predawn water potential). Cluster analysis found that δ13C values are likely affected by the differing phenology of each variety, resulting in berry ripening of different varieties taking place under different stress conditions within the same year. We accounted for these phenological differences and found that cluster analysis based on specific δ13C metrics created a classification of varieties that corresponds well to our current empirical understanding of their relative drought tolerances. In addition, we analysed the water potential regulation of the subset of six varieties (using the hydroscape approach) and found that it was well correlated with some δ13C metrics. Surprisingly, a variety’s water potential regulation (specifically its minimum critical leaf water potential under water deficit) was strongly correlated to δ13C values under well-watered conditions, suggesting that base WUE may have a stronger impact on drought tolerance than WUE under water deficit. These results give strong insights on the innate WUE of a very large panel of varieties and suggest that studies of drought tolerance should include traits expressed under non-limiting conditions.

Un vasto programma si riferisce alla verifica di 28 selezioni clonali di Pinot nero atte a vini base spumante. Gli impianti sono stati realizzati in diversi ambienti delle Langhe e del Monferrato nel periodo 1992-1996, in 57 vigneti diversi e su una superficie complessiva di circa 50 Ha.