Macrowine 2021
IVES 9 IVES Conference Series 9 The valorization of wine lees as a source of mannoproteins for food and wine applications

The valorization of wine lees as a source of mannoproteins for food and wine applications

Abstract

AIM. Wine yeast lees constitute a winemaking by-product that, unlike grape skins and seeds, are not sufficiently exploited to add value to the winemaking sector, as their treatment and disposal generally represents a cost for wineries [1]. Recently, some valorization strategies proposed the integrated extraction of ethanol, polyphenols, and tartaric acid, while only a few studies investigated ways to exploit the remaining wine lees’ yeast biomass. In particular, no studies attempted the extraction of mannoproteins (MPs), yeast cell wall polysaccharides with known foaming, emulsifying and wine-stabilizing activities [2], from the wine lees’ yeast biomass. To fill this gap, this research aims at developing an efficient and food-grade method for the extraction of yeast MPs from commercial wine lees, and to test the obtained extracts as wine stabilizers, foaming agents, and food emulsifiers.

METHODS. Several protocols were studied to extract MPs from wine lees. Ultimately, commercial wine yeast lees were extracted at pH 3.4 using an autoclave-based treatment (121°C, 20 min). The obtained MPs extracts were characterized by SEC-HPLC, SDS-PAGE or CI-ELLSA [3]. The functionalities of the MPs’ extracts were tested in wine by assessing their foam-promoting ability and their stabilizing potential against protein and tartrate instabilities. Additionally, MPs extracts were tested as emulsifying and foaming agents in model food matrices. The results were compared to those obtained using commercial MPs-based products and/or MPs extracts from pure cultures of the same yeast strains.

RESULTS. Among the extraction protocols tested, the autoclave emerged as the best performing in terms of extract’s effectiveness and, therefore, it was selected for the subsequent extractions. Firstly, MPs obtained from white winemaking lees positively impacted both wine’s foaming properties (+260% height; +360% stability) and tartrate stability (-11%) compared to untreated wine samples. Conversely, the extracts were ineffective in stabilizing wine against protein haze formation [4]. Subsequently, MPs extracts obtained autoclaving red and white wine lees and tested in model food matrices showed encouraging emulsifying activity (≃55% emulsion stability) and foaming properties (stability >3h). In this case, the extract from red wine lees performed better than its analog derived from the same yeast strain grown in the laboratory, thus suggesting a possible impact of wine polyphenols in enhancing the surfactant action of MPs [5].

CONCLUSIONS

The extraction of MPs from wine lees with a simple and food-grade autoclave-based method can represent an effective valorization strategy that, if integrated with the already available techniques to recover ethanol, tartaric acid, and polyphenols, would result in a better exploitation of this by-product with a consequent improvement of the environmental and economic sustainability of the wine industry.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Alberto De Iseppi

Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Italy, Andrea CURIONI1,2; Matteo MARANGON1; Giovanna LOMOLINO1; Simone VINCENZI1,2; Benoit DIVOL3

¹ Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Italy
² Centre for Research in Viticulture and Enology (CIRVE), Conegliano, Italy
³ South African Grape and Wine Research Institute, Stellenbosch University, South Africa

Contact the author

Keywords

wine yeast lees, by-product valorisation, mannoproteins, stability, foam, emulsion

Citation

Related articles…

Diagnosis of soil quality and evaluation of the impact of viticultural practices on soil biodiversity in a vineyard in southwestern France

Viticulture is facing two major changes – climate change and agroecological transition. In both cases, soil quality is seen as a lever to move towards a more sustainable viticulture. However, soil biological quality is little considered in the implementation of viticultural practices. Gascogn’Innov (2017-2022) is an Operational Group funded by the European Innovation Partnership for Agriculture. As such, it brings together winegrowers from the south-west of France, scientists, advisors and technicians, around a project focused on viticultural soil biological functioning and the design of technical routes more respectful toward soil heritage. To achieve this, the project aims to acquire references on the impact of viticultural practices on soil biology from a dynamic way, and to test a methodology to integrate information provided by the soil bioindicators to manage farming systems. A set of indicators of soil biological quality are evaluated in the project: microorganisms (bacteria and fungi abundance and diversity), fauna (abundance and diversity of nematodes and earthworms), physico-chemical characteristics, soil structure assessment and degradation rate of organic matter. Based on a network of 13 plots that have been subject to an initial diagnosis in 2017, several agronomical practices to restore soil fertility are experimented to redesign the cropping system (for instance plant cover, organic matter inputs, reduction of herbicides, mineral fertilizers). System redesign was made in collaboration by winegrowers and an interdisciplinary group of experts (agronomists, biologists). Several indicators are measured on vine and soil at each vintage to assess vine health and productivity. At the end of the project (2021), a final diagnosis was carried out. Gascogn’Innov allowed to create a regional database on the quality of wine-growing soils, which permitted to evaluate the effect of practices according to soil types. Especially, decreasing the intensity of tillage and increasing the duration and diversity of grass coverage tends to increase the abundance of all the organisms studied. This project confirmed the value of soil biological quality indicators to drive the sustainability of practices, but also highlighted the key-role of expertise, in both agronomy and soil biology, to help winegrowers understand and appropriate their soil quality diagnoses.

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…

How distinctive are single vineyard Gewürztraminer musts and wines from Alto Adige (Italy) based on untargeted analysis, sensory profiling, and chemometric elaboration?

Vitis vinifera L. ‘Gewürztraminer’ is a historical grape variety of Alto Adige (Südtirol), Italy, which is widely grown in the area of Tramin an der Weinstraße, but is also grown globally. It produces highly aromatic wines that are strongly influenced by the terroir of the vineyard sites where they are grown. This study looked at musts and young wines from ‘Gewürztraminer’ grapes harvested in seven distinct vineyards near Tramin and then processed at Cantina di Termeno, minimizing winemaking protocol variability. Samples were profiled using bidimensional gas chromatography–time-of-flight mass spectrometry, liquid chromatography coupled to electrochemical detection, and near-IR spectrometry. The data were subjected to Principle Component Analysis and Hierarchical Clustering Analysis. Sensory discriminant testing was undertaken using the sorting method with a semi-trained panel, and the data were processed using Multidimensional Scaling. Seven must/wine pairs could be distinguished based on their untargeted volatilome profiles and on sensory evaluation. As expected, there were greater differences in the volatile compounds between the wines than between the musts. The wines from vineyards 4 and 5 were nonetheless quite homogenous in terms of chemical and sensory analyses, as were the wines from vineyards 1 and 3. For the phenolic profile, differences were noted between the musts and wines of vineyards 2, 3, and 4, but the musts from vineyards 5 and 7 were similar. Sensory analysis showed the wines from vineyards 6 and 7 to be distinct from the rest. These results reinforce that the composition of ‘Gewürztraminer’ musts and wines is strongly determined by vineyard site, even in a small geographic area with high variability of the terroir (soil and microclimate), and that these differences are apparent in the flavours and aromas of the finished wines. Further confirmation would require a larger sample of wines, preferably from several vintages.

Adaptability of grapevines to climate change: characterization of phenology and sugar accumulation of 50 varieties, under hot climate conditions

Climate is the major factor influencing the dynamics of the vegetative cycle and can determine the timing of phenological periods. Knowledge of the phenology of varieties, their chronological duration, and thermal requirements, allows not only for the better management of interventions in the vineyard, but also to predict the varieties’ behaviour in a scenario of climate change, giving the wine producer the possibility of selecting the grape varieties that are best adapted to the climatic conditions of a certain terroir. In 2014, Symington Family Estates, Vinhos, established two grape variety libraries in two different places with distinctive climate conditions (Douro Superior, and Cima Corgo), with the commitment of contributing to a deeper agronomic and oenological understanding of some grape varieties, in hot climate conditions. In these research vineyards are represented local varieties that are important in the regional and national viticulture, but also others that have over time been forgotten — as well as five international reference cultivars. From 2017 to 2021, phenological observations have been made three times a week, following a defined protocol, to determine the average dates of budbreak, flowering and veraison. With the climate data of each location, the thermal requirements of each variety and the chronological duration of each phase have been calculated. During maturation, berry samples have been gathered weekly to study the dynamics of sugar accumulation, between other parameters. The data was analysed applying phenological and sugar accumulation models available in literature. The results obtained show significant differences between the varieties over several parameters, from the chronological duration and thermal requirements to complete the various stages of development, to the differences between the two locations, confirming the influence of the climate on phenology and the stages of maturation, in these specific conditions.

First step in the preparation of a soil map of the Protected Designation of Origin Valdepeñas (Central, Spain)

This work is a first step to make a map of vineyard soils. The characterization of the soils of the Protected Designation of Origin (D.P.O.) Valdepeñas will allow to group the studied profiles according to their physico-chemical characteristics and the concentrations of most relevant chemical elements. 90 soil profiles were analysed throughout the territory and the soils were sampled and described according to FAO (2006) and classified according to and Soil Taxonomy (2014). All samples were air dried, sieved and some physico-chemical parameters were determined following standard protocols. Also, major and trace elements were analysed by X-ray fluorescence. The statistically study was made using the SPSS program. Trend maps were made using the ArcGIS program. The studied soils have the following average properties: pH, 8.3; electrical conductivity, 0,20 dS/m (low); clay, 18.8% (medium) and CaCO3, 17.1% (high). In the study for the major elements. The major elements of these soils are Si, followed by Ca and Al, with an average content of 203.7 g/kg, 105.5 g/kg and 74.0 g/kg respectively. On the other hand, 27 trace elements have been studied. Of all of them, it can be highlighted the average values of Ba (361.8 mg/kg), Sr (129.3 mg/kg), Rb (83.4 mg/kg), V (74.2 mg/kg) and Ce (70.6 mg/kg). Ba, V and Ce values are higher and the values of Sr and Rb are lower to those found in the literature. The discriminant analysis shows a percentage of grouping of 91%. The content of chemical elements together with the physico-chemical characteristics allows grouping the soils in 4 group according to their order in the classification to Soil Taxonomy; due to the importance of the Calcisols in Castilla-La Mancha, it has been decided to establish them as their own group even if they do not appear in Soil Taxonomy classification.