Macrowine 2021
IVES 9 IVES Conference Series 9 Microwave-assisted maceration and stems addition in Bonarda grapes: effects on wine chemical composition and sensory properties over two vintages

Microwave-assisted maceration and stems addition in Bonarda grapes: effects on wine chemical composition and sensory properties over two vintages

Abstract

AIM: Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, wines with low levels of quality compounds. Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods. Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost. Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition and sensory properties of Bonarda wines.

METHODS: During two consecutive vintages (2018-2019), 450 kg of grapes were harvested (≈24°Brix) from a commercial vineyard (Mendoza, Argentina), and made into wine in 25 L following a standard protocol. The experimental design consisted of ten treatments (two factors) by triplicate. Two maceration strategies were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50°C)], combined with five stem-contact conditions [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60°C), 100% stems addition (S100), 100% stems addition + MW (S100MW)]. Wines were analyzed for basic chemistry (1), phenolic composition and color parameters (2-5), polysaccharides (6), and aroma profiles (7). Additionally, a descriptive sensory analysis (QDA) was performed with 19 panelists in 8 sessions, and 22 attributes were established.

RESULTS: In both seasons, the application of microwaves significantly reduced microbial flora in musts (fungi, yeasts, and acetic acid bacteria), in addition to inhibiting enzymatic activity (cellulase and pectinase). Due to the significant difference of the vintage and its interaction with some of the studied factors, the chemical and sensory characterization of wines were evaluated separately for each season. The 2018 wines showed higher pH with stem additions and MW application in both matrices. Stem additions increased tannin content by 63% (S100) and by >35% for the other treatments; while MW consistently improved phenolic extraction (mainly, anthocyanins and derivatives), and polymeric pigments formation. Likewise, combined strategies increased polysaccharides extraction (FI, 165 kDa; FII, 45 kDa; FIII, 12 kDa), enhanced wine color (greater saturation), and intensified violet hue. Finally, the PCA including sensory variables described the MWS50 wines with higher color intensity and chocolate aroma, and 100% stems addition treatments with more astringency and violet hue. The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).

CONCLUSIONS:

The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed technological strategies on the chemical and sensory quality of red wines.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Martín Fanzone 

Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Universidad Juan Agustín Maza, Av. Acceso Este Lateral Sur 2245, CP5519, Guaymallén, Mendoza, Argentina.,Ignacio Coronado. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Santiago Sari. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Anibal Catania. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Mariona Gil i Cortiella. Instituto de Ciencias Químicas Aplicadas, Universidad Autónoma de Chile, Santiago 8910060, Chile. Cristina Ubeda. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González 2, 41012 Sevilla, Spain. Instituto de Ciencias Biomédicas, Facultad de Ciencias, Universidad Autónoma de Chile, Santiago 8910060, Chile. Mariela Assof. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Universidad Juan Agustín Maza, Av. Acceso Este Lateral Sur 2245, CP5519, Guaymallén, Mendoza, Argentina. Viviana Jofré. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Universidad Juan Agustín Maza, Av. Acceso Este Lateral Sur 2245, CP5519, Guaymallén, Mendoza, Argentina. Vilma Morata de Ambrosini. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Facultad de Ciencias Aplicadas a la Industria, Universidad Nacional de Cuyo, Bernardo de Irigoyen 375, 5600, Mendoza, Argentina. Alvaro Peña Neira. Facultad de Ciencias Agronómicas, Universidad de Chile, Avenida Santa Rosa 11315, Santiago 8820808, Chile.

Contact the author

Keywords

microwave-assisted extraction, stems, bonarda, phenolics, polysaccharides, aromas, sensory analysis

Citation

Related articles…

Downscaling of remote sensing time series: thermal zone classification approach in Gironde region

In viticulture, the challenges of local climate modelling are multiple: taking into account the local environment, fine temporal and spatial scales, reliable time series of climate data, ease of implementation and reproducibility of the method. At the local scale, recent studies have demonstrated the contribution of spatialization methods for ground-based climate observation data considering topographic factors such as altitude, slope, aspect, and geographic coordinates (Le Roux et al, 2017; De Rességuier et al, 2020). However, these studies have shown questions in terms of the reproducibility and sustainability of this type of climate study. In this context, we evaluated the potential of MODIS thermal satellite images validated with ground-based climate data (Morin et al, 2020). Previous studies have been encouraging, but questions remain to be explored at the regional scale, particularly in the dynamics of the massive use of bioclimatic indices to classify the climate of wine regions. The results at the local scale were encouraging, but this approach was tested in the current study at the regional scale. Several objectives were set: 1) to evaluate the downscaling method for land surface temperature time series, 2) to identify regional thermal structure variations. We used weekly minimum and maximum surface temperature time series acquired by MODIS satellites at a spatial resolution of 1000 m and downscaled at 500 m using topographical variables. Two types of analyses were performed:

Effect of vigour and number of clusters on eonological parameters and metabolic profile of Cabernet Sauvignon red wines

Vegetative growth and yield are reported to affect grape and wine quality. They can be controlled through different techniques linked to vine management. The objective of this research was to determine the effect of vine vigour and number of clusters per vine on physicochemical composition and phenolic profile of red wines. The experiment was carried out during two vegetative cycles, with cv. Cabernet Sauvignon grafted onto Paulsen 1103. Three vine vigour were defined, according to shoot weight at previous harvests, being low, medium and high. Five treatments of number of clusters were used for each vigour, with 15, 22, 29, 36, and 45 clusters per vine. Grapes from all treatments were harvested in the same day from Brix and total acidity criteria. Thirty days after bottling, classical analyzes and phenolic compounds were performed. As results, different responses were obtained from each vintage. In 2020, a dry season from veraison to harvest, grapes and wines obtained from low vigour treatment and 45 clusters per vine was the highest in sugar and alcohol content respectively, while grapes and wines from high vigour and 15 clusters presented the lowest sugar and alcohol content. Total anthocyanins were higher in treatment with low vigour and 15 clusters, while the lowest amounts were found in low vigour with 45 clusters, as well as medium and high vigour with 36 clusters per vine. Total tannins were higher in high vigour with 22 clusters and medium vigour with 29 clusters, while were lower in low vigour with 36 clusters. In 2021, a wet season at harvest, responses were different, and great variations were observed between treatments. As conclusions, yield and vine vigour had strong influence on grape and wine quality, promoting different enological potentials on which can be indicated/used for aging strategies of red and even rosé wines.

The potential of multispectral/hyperspectral technologies for early detection of “flavescence dorée” in a Portuguese vineyard

“Flavescence dorée” (FD) is a grapevine quarantine disease associated with phytoplasmas and transmitted to healthy plants by insect vectors, mainly Scaphoideus titanus. Infected plants usually develop symptoms of stunted growth, unripe cane wood, leaf rolling, leaf yellowing or reddening, and shrivelled berries. Since plants can remain symptomless up to four years, they may act as reservoirs of FD contributing to the spread of the disease. So far, conventional management strategies rely mainly on the insecticide treatments, uprooting of infected plants and use of phytoplasma-free propagation material. However, these strategies are costly and could have undesirable environmental impacts. Thus, the development of sustainable and noninvasive approaches for early detection of FD and its management are of great importance to reduce disease spread and select the best cultural practices and treatments. The present study aimed to evaluate if multispectral/hyperspectral technologies can be used to detect FD before the appearance of the first symptoms and if infected grapevines display a spectral imaging fingerprint. To that end, physiological parameters (leaf area, chlorophyll content and photosynthetic rate) were collected in concomitance to the measurements of plant reflectance (using both a portable apparatus and a remote sensing drone). Measurements were performed in two leaves of 8 healthy and 8 FD-infected grapevines, at four timepoints: before the development of disease symptoms (21st June); and after symptoms appearance (ii) at veraison (2nd August); at post-veraison (11th September); and at harvest (25th September). At all timepoints, FD infected plants revealed a significant decrease in the studied physiological parameters, with a positive correlation with drone imaging data and portable apparatus analyses. Moreover, spectra of either drone imaging and portable apparatus showed clear differences between healthy and FD-infected grapevines, validating multispectral/ hyperspectral technology as a potential tool for the early detection of FD or other grapevine-associated diseases.

Adaptability of grapevines to climate change: characterization of phenology and sugar accumulation of 50 varieties, under hot climate conditions

Climate is the major factor influencing the dynamics of the vegetative cycle and can determine the timing of phenological periods. Knowledge of the phenology of varieties, their chronological duration, and thermal requirements, allows not only for the better management of interventions in the vineyard, but also to predict the varieties’ behaviour in a scenario of climate change, giving the wine producer the possibility of selecting the grape varieties that are best adapted to the climatic conditions of a certain terroir. In 2014, Symington Family Estates, Vinhos, established two grape variety libraries in two different places with distinctive climate conditions (Douro Superior, and Cima Corgo), with the commitment of contributing to a deeper agronomic and oenological understanding of some grape varieties, in hot climate conditions. In these research vineyards are represented local varieties that are important in the regional and national viticulture, but also others that have over time been forgotten — as well as five international reference cultivars. From 2017 to 2021, phenological observations have been made three times a week, following a defined protocol, to determine the average dates of budbreak, flowering and veraison. With the climate data of each location, the thermal requirements of each variety and the chronological duration of each phase have been calculated. During maturation, berry samples have been gathered weekly to study the dynamics of sugar accumulation, between other parameters. The data was analysed applying phenological and sugar accumulation models available in literature. The results obtained show significant differences between the varieties over several parameters, from the chronological duration and thermal requirements to complete the various stages of development, to the differences between the two locations, confirming the influence of the climate on phenology and the stages of maturation, in these specific conditions.

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.