Macrowine 2021
IVES 9 IVES Conference Series 9 Microwave-assisted maceration and stems addition in Bonarda grapes: effects on wine chemical composition and sensory properties over two vintages

Microwave-assisted maceration and stems addition in Bonarda grapes: effects on wine chemical composition and sensory properties over two vintages

Abstract

AIM: Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, wines with low levels of quality compounds. Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods. Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost. Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition and sensory properties of Bonarda wines.

METHODS: During two consecutive vintages (2018-2019), 450 kg of grapes were harvested (≈24°Brix) from a commercial vineyard (Mendoza, Argentina), and made into wine in 25 L following a standard protocol. The experimental design consisted of ten treatments (two factors) by triplicate. Two maceration strategies were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50°C)], combined with five stem-contact conditions [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60°C), 100% stems addition (S100), 100% stems addition + MW (S100MW)]. Wines were analyzed for basic chemistry (1), phenolic composition and color parameters (2-5), polysaccharides (6), and aroma profiles (7). Additionally, a descriptive sensory analysis (QDA) was performed with 19 panelists in 8 sessions, and 22 attributes were established.

RESULTS: In both seasons, the application of microwaves significantly reduced microbial flora in musts (fungi, yeasts, and acetic acid bacteria), in addition to inhibiting enzymatic activity (cellulase and pectinase). Due to the significant difference of the vintage and its interaction with some of the studied factors, the chemical and sensory characterization of wines were evaluated separately for each season. The 2018 wines showed higher pH with stem additions and MW application in both matrices. Stem additions increased tannin content by 63% (S100) and by >35% for the other treatments; while MW consistently improved phenolic extraction (mainly, anthocyanins and derivatives), and polymeric pigments formation. Likewise, combined strategies increased polysaccharides extraction (FI, 165 kDa; FII, 45 kDa; FIII, 12 kDa), enhanced wine color (greater saturation), and intensified violet hue. Finally, the PCA including sensory variables described the MWS50 wines with higher color intensity and chocolate aroma, and 100% stems addition treatments with more astringency and violet hue. The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).

CONCLUSIONS:

The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed technological strategies on the chemical and sensory quality of red wines.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Martín Fanzone 

Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Universidad Juan Agustín Maza, Av. Acceso Este Lateral Sur 2245, CP5519, Guaymallén, Mendoza, Argentina.,Ignacio Coronado. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Santiago Sari. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Anibal Catania. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Mariona Gil i Cortiella. Instituto de Ciencias Químicas Aplicadas, Universidad Autónoma de Chile, Santiago 8910060, Chile. Cristina Ubeda. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González 2, 41012 Sevilla, Spain. Instituto de Ciencias Biomédicas, Facultad de Ciencias, Universidad Autónoma de Chile, Santiago 8910060, Chile. Mariela Assof. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Universidad Juan Agustín Maza, Av. Acceso Este Lateral Sur 2245, CP5519, Guaymallén, Mendoza, Argentina. Viviana Jofré. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Universidad Juan Agustín Maza, Av. Acceso Este Lateral Sur 2245, CP5519, Guaymallén, Mendoza, Argentina. Vilma Morata de Ambrosini. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Facultad de Ciencias Aplicadas a la Industria, Universidad Nacional de Cuyo, Bernardo de Irigoyen 375, 5600, Mendoza, Argentina. Alvaro Peña Neira. Facultad de Ciencias Agronómicas, Universidad de Chile, Avenida Santa Rosa 11315, Santiago 8820808, Chile.

Contact the author

Keywords

microwave-assisted extraction, stems, bonarda, phenolics, polysaccharides, aromas, sensory analysis

Citation

Related articles…

The plantation frame as a measure of adaptation to climate change

The mechanization of vineyard work originally led to a reduction in planting densities due to the lack of machinery adapted to the vineyard. The current availability of specific machinery makes it possible to establish higher planting densities. In this work, three planting densities (1.40×0.80 m, 1.80×1 m and 2.20×1.20 m, corresponding to 8928, 5555 and 3787 plants/ha respectively) were studied with four varieties autochthonous of Galicia (northwestern Spain): Albariño and Treixadura (white), Sousón and Mencía (red). The vines were trained in a vertical shoot positioning system using a single Royat cordon, and pruned to spurs with two buds each. Agronomic data (yield, pruning wood weight, Ravaz index) and oenological data in must were collected. The higher planting density (1.40×0.80 m) had no significant effect on grape yield per vine in white varieties, although production per hectare was much higher due to the greater number of plants. In red varieties, this planting density resulted in a significantly lower production per vine, compensated by the greater number of plants. In addition, it significantly reduced the Brix degree in the must of the Albariño, Treixadura and Sousón varieties, and increased the total acidity in the latter two and Mencía. It also caused an increase in extractable and total anthocyanins and IPT in red grapes. The effects of high planting density on grapes are of great interest for the adaptation of varieties in the context of climate change. In the future, it could be advisable to modify the limits imposed by the appellations of origin on the planting density of these varieties in order to obtain more balanced wines.

Mobile device to induce heat-stress on grapevine berries

Studying heat stress response of grapevine berries in the field often relies on weather conditions during the growing season. We constructed a mobile heating device, able to induce controlled heat stress on grapes in vineyards. The heater consisted of six 150 W infrared lamps mounted in a profile frame. Heating power of the lamps could be controlled individually by a control unit consisting of a single board computer and six temperature sensors to reach a pre-set temperature. The heat energy applied to individual berries within a cluster decreases by the squared distance to the heat source, enabling the establishment of temperature profiles within individual clusters. These profiles can be measured by infrared thermography once a steady state has been reached. Radiant flux density received by a berry depending on the distance was calculated based on a view factor and measured lamp surface temperature and resulted to 665 Wm-2 at 7cm. Infrared thermography of the fruit surface was in good agreement with measurements conducted with a thermocouple inserted at epidermis level. In combination with infrared thermography, the presented device offers possibilities for a wide range of applications like phenotyping for heat tolerance in the field to proceed in the understanding of the complex response of plants to heat stress. Sunburn necrosis symptoms were artificially induced with the aid of the device for cv. Bacchus and cv. Sylvaner in the 2020 and 2021 growing season. Threshold temperatures for sunburn induction (LT5030min) were derived from temperature data of single berries and visual sunburn assessment, applying logistic regression. A comparison of threshold temperatures for the occurrence of sunburn necrosis confirmed the higher susceptibility of cv. Bacchus. The lower susceptibility of cv. Sylvaner did not seem to be related to its phenolic composition, rendering a thermoprotective role of berry phenolic compounds unlikely.

Updating the Winkler index: An analysis of Cabernet sauvignon in Napa Valley’s varied and changing climate

This study aims to create an updated, agile viticultural climate index (similar to the Winkler Index) by performing in-depth analyses of current and historical data from industry partners in several major winegrowing regions. The Winkler Index was developed in the early twentieth century based on analysis of various grape-growing regions in California. The index uses heat accumulation (i.e. Growing Degree Days) throughout the growing season to determine which grape varieties are best suited to each region. As viticultural regions are increasingly subject to the complexity and uncertainty of a changing climate, a more rigorous, agile model is needed to aid grape growers in determining which cultivars to plant where. For the first phase of this study, 21 industry partners throughout Napa Valley shared historical phenology, harvest, viticultural practice, and weather data related to their Cabernet sauvignon vineyard blocks. To complement this data, berry samples were collected throughout the 2021 growing season from 50 vineyard blocks located throughout 16 American Viticultural Areas that were then analyzed for basic berry chemistry and phenolics. These blocks have been mapped using a Geographic Information System (GIS), enabling analysis of altitude, vineyard row orientation, slope, and remotely sensed climate data. Sampling sites were also chosen based on their proximity to a weather station. By analyzing historical data from industry partners and data specifically collected for this study, it is possible to identify key parameters for further analysis. Initial results indicate extreme variability at a high spatial resolution not currently accounted for in modern viticultural climate indices and suggest that viticultural practices play a major role. Using the structure of data collection and analyses developed for the first phase, this project will soon be expanded to other wine regions globally, while continuing data collection in Napa Valley.

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard

20-Year-Old data set: scion x rootstock x climate, relationships. Effects on phenology and sugar dynamics

Global warming is one of the biggest environmental, social, and economic threats. In the Douro Valley, change to the climate are expected in the coming years, namely an increase in average temperature and a decrease in annual precipitation. Since vine cultivation is extremely vulnerable and influenced by the climate, these changes are likely to have negative effects on the production and quality of wine.
Adaptation is a major challenge facing the viticulture sector where the choice of plant material plays an important role, particularly the rootstock as it is a driver for adaptation with a wide range of effects, the most important being phylloxera, nematode and salt, tolerance to drought and a complex set of interactions in the grafted plant.
In an experimental vineyard, established in the Douro Region in 1997, with four randomized blocs, with five varieties, Touriga Nacional, Tinta Barroca, Touriga Franca and Tinta Roriz, grafted in four rootstocks, Rupestris du Lot, R110, 196-17C, R99 and 1103P, data was collected consecutively over 20 years (2001-2020). Phenological observations were made two to three times a week, following established criteria, to determine the average dates of budbreak, flowering and veraison. During maturation, weekly berry samples were taken to study the dynamics of sugar accumulation, amongst other parameters. Climate data was collected from a weather station located near the vineyard parcel, with data classified through several climatic indices.
The results achieved show a very low coefficient of variations in the average date of the phenophases and an important contribution from the rootstock in the dynamic of the phenology, allowing a delay in the cycle of up to10-12 days for the different combinations. The Principal Component Analysis performed, evaluating trends in the physical-chemical parameters, highlighted the effect of the climate and rootstock on fruit quality by grape varieties.