Macrowine 2021
IVES 9 IVES Conference Series 9 Microwave-assisted maceration and stems addition in Bonarda grapes: effects on wine chemical composition and sensory properties over two vintages

Microwave-assisted maceration and stems addition in Bonarda grapes: effects on wine chemical composition and sensory properties over two vintages

Abstract

AIM: Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, wines with low levels of quality compounds. Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods. Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost. Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition and sensory properties of Bonarda wines.

METHODS: During two consecutive vintages (2018-2019), 450 kg of grapes were harvested (≈24°Brix) from a commercial vineyard (Mendoza, Argentina), and made into wine in 25 L following a standard protocol. The experimental design consisted of ten treatments (two factors) by triplicate. Two maceration strategies were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50°C)], combined with five stem-contact conditions [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60°C), 100% stems addition (S100), 100% stems addition + MW (S100MW)]. Wines were analyzed for basic chemistry (1), phenolic composition and color parameters (2-5), polysaccharides (6), and aroma profiles (7). Additionally, a descriptive sensory analysis (QDA) was performed with 19 panelists in 8 sessions, and 22 attributes were established.

RESULTS: In both seasons, the application of microwaves significantly reduced microbial flora in musts (fungi, yeasts, and acetic acid bacteria), in addition to inhibiting enzymatic activity (cellulase and pectinase). Due to the significant difference of the vintage and its interaction with some of the studied factors, the chemical and sensory characterization of wines were evaluated separately for each season. The 2018 wines showed higher pH with stem additions and MW application in both matrices. Stem additions increased tannin content by 63% (S100) and by >35% for the other treatments; while MW consistently improved phenolic extraction (mainly, anthocyanins and derivatives), and polymeric pigments formation. Likewise, combined strategies increased polysaccharides extraction (FI, 165 kDa; FII, 45 kDa; FIII, 12 kDa), enhanced wine color (greater saturation), and intensified violet hue. Finally, the PCA including sensory variables described the MWS50 wines with higher color intensity and chocolate aroma, and 100% stems addition treatments with more astringency and violet hue. The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).

CONCLUSIONS:

The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed technological strategies on the chemical and sensory quality of red wines.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Martín Fanzone 

Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Universidad Juan Agustín Maza, Av. Acceso Este Lateral Sur 2245, CP5519, Guaymallén, Mendoza, Argentina.,Ignacio Coronado. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Santiago Sari. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Anibal Catania. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Mariona Gil i Cortiella. Instituto de Ciencias Químicas Aplicadas, Universidad Autónoma de Chile, Santiago 8910060, Chile. Cristina Ubeda. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González 2, 41012 Sevilla, Spain. Instituto de Ciencias Biomédicas, Facultad de Ciencias, Universidad Autónoma de Chile, Santiago 8910060, Chile. Mariela Assof. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Universidad Juan Agustín Maza, Av. Acceso Este Lateral Sur 2245, CP5519, Guaymallén, Mendoza, Argentina. Viviana Jofré. Estación Experimental Mendoza, Instituto Nacional de Tecnología Agropecuaria, San Martín 3853, M5528AHB, Luján de Cuyo, Mendoza, Argentina. Universidad Juan Agustín Maza, Av. Acceso Este Lateral Sur 2245, CP5519, Guaymallén, Mendoza, Argentina. Vilma Morata de Ambrosini. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Facultad de Ciencias Aplicadas a la Industria, Universidad Nacional de Cuyo, Bernardo de Irigoyen 375, 5600, Mendoza, Argentina. Alvaro Peña Neira. Facultad de Ciencias Agronómicas, Universidad de Chile, Avenida Santa Rosa 11315, Santiago 8820808, Chile.

Contact the author

Keywords

microwave-assisted extraction, stems, bonarda, phenolics, polysaccharides, aromas, sensory analysis

Citation

Related articles…

Long-term drought resilience of traditional red grapevine varieties from a semi-arid region

In recent decades, the scarcity of water resources in agriculture in certain areas has been aggravated by climate change, which has caused an increase in temperatures, changes in rainfall patterns, as well as an increase in the frequency of extreme phenomena such as droughts and heat waves. Although the vine is considered a drought-tolerant specie, it has to satisfy important water requirements to complete its cycle, which coincides with the hottest and driest months. Achieving sustainable viticulture in this scenario requires high levels of efficiency in the use of water, a scarce resource whose use is expected to be severely restricted in the near future. In this regard, the use of drought-tolerant varieties that are able to maintain grape yield and quality could be an effective strategy to face this change. During three consecutive seasons (2018-2020) the behavior in rainfed regime of 13 traditional red grapevine varieties of the Spain central region was studied. These varieties were cultivated in a collection at Centro de Investigación de la Vid y el Vino de Castilla-La Mancha (IVICAM-IRIAF) located in Tomelloso (Castilla-La Mancha, Spain). Yield components (yield, mean bunch and berry weight, pruning weight), physicochemical parameters of the musts (brix degree, total acidity, pH) and some physiological parameters related with water stress during ripening period (δ13C, δ18O) were analysed. The application of different statistical techniques to the results showed the existence of significant differences between varieties in their response to stressful conditions. A few varieties highlighted for their high ability to adapt to drought, being able to maintain high yields due to their efficiency in the use of water. In addition, it was possible quantify to what extent climate can be a determinant in the δ18O of musts under severe water stress conditions.

Organic recycled mulches in sustainable viticulture: assessment of spontaneous plants communities and weed coverage

In recent years, developing more efficient and sustainable viticulture management has been essential due to the impact of climate change in semiarid regions. For this reason, the use of recycled organic mulching (ROM) in the vineyard has become an interesting strategy to cope with water stress, isolated soil from extreme temperatures and improving soil humidity, control the presence of weeds and therefore reduce the inputs of herbicides and improve soil fertility. This work aimed to analyse the effect of three different organic mulches [straw (S), grape pruning debris (GPD) and spent mushroom compost (SMC)] and two traditional soil management techniques [herbicide (H) and interrow (IN)] on weed coverage and the spontaneous plant communities’ presence. Data sampling was collected throughout the vine vegetative cycle of 2021 in La Rioja, Spain. The different soil management techniques had a clear effect on weed coverage and his development during the vine vegetative cycle. SMC and H were the treatments with the highest and the lowest coverage percentage, respectively. IN had a delayed weed emergence at the beginning of the vine vegetative cycle, but finally it reached maximum values nearby SMC. GPD and S had similar effects on weed emergence, reaching 25-30% of the maximum coverage values. A total of 29 herbaceous species were identified during the vegetative cycle, some of them very isolated and occasional. Principal component analysis (PCAs) showed a good association between spontaneous species and treatments, furthermore, specific species-treatment associations were found. Moreover, three clear groups of herbaceous communities were identified by cluster analysis. This study provides interesting information about the effect of different alternative soil management on herbaceous plant coverage and weed species communities which could contribute to making more sustainable viticulture.

Assessing the relationship between cordon strangulation, dieback, and fungal trunk disease symptom expression

Grapevine trunk diseases including Eutypa dieback are a major factor in the decline of vineyards and may lead to loss of productivity, reduced income, and premature reworking or replanting. Several studies have yielded results indicating that vines may be more likely to express symptoms of vascular disease if their health is already compromised by stress. In Australia and many other wine-growing regions it is a common practice for canes to be wrapped tightly around the cordon wire during the establishment of permanent cordon arms. It is likely that this practice may have a negative effect on health and longevity, as older cordons that have been trained in this manner often display signs of decay and dieback, with the wire often visibly embedded within the wood of the cordon. It is possible that adopting a training method which avoids constriction of the vasculature of the cordon may help to limit the onset of vascular disease symptom expression. A survey was conducted during the spring of two consecutive growing seasons on vineyards in South Australia displaying symptoms of Eutypa lata infection when symptomless shoots were 50–100 cm long. Vines were assessed as follows: (i) the proportion of cordon exhibiting dieback was rated using a 0–100% scale; (ii) the proportion of canopy exhibiting foliar symptoms of Eutypa dieback was rated using a 0–100% scale; (iii) the severity of strangulation was rated using a 0–4 point scale. Images were also taken of each vine for the purpose of measuring plant area index (PAI) using the VitiCanopy App. The goal of the survey was to determine if and to what extent any correlation exists between severity of strangulation and cordon dieback, in addition to Eutypa dieback foliar symptom expression.

Mechanisms involved in the heating of the environment by the aerodynamic action of a wind machine to protect a vineyard against spring frost

One of the main consequences of global warming is the rise of the mean temperature. Thus, the heat summation by the plants begins sooner in the early spring, and by cumulating growing degree-days, phenological development tends to happen earlier. However, spring frost is still a recurrent phenomenon causing serious damages to buds and therefore, threatening the harvests of the winegrowers. The wind machine is a solution to protect fruit crops against spring frost that is increasingly used. It is composed of a 10-m mast with a blowing fan at its peak. By tapping into the strength of the nocturnal thermal inversion, it sweeps the crop by propelling warm air above to the ground. Thus, stratification is momentarily suppressed. Furthermore, the continuous action of the machine, alone or in synergy, or the addition of a heater allow the bud to be bathed in a warmer environment. Also, the punctual action of the tower’s warm gust reaches the bud directly at each rotation period. All these actions allow the bud to continuously warm up, but with different intensities and over a different period. Although there is evidence of the effectiveness of the wind machines, the thermal transfers involved in those mechanisms raise questions about their true nature. Field measurements based on ultrasonic anemometers and fast responding thermocouples complemented by laboratory measurements on a reduced scale model allow to characterize both the airflow produced by the wind machine and the local temperature in its vicinity. Those experiments were realized in the vineyard of Quincy, in the framework of the SICTAG project. In the future paper, we will detail the aeraulic characterization of the wind machine and the thermal effects resulting from it and we will focus on how the wind machine warms up the local atmosphere and enables to reduce the freezing risk.

Phenolic composition of Tempranillo Blanco grapes changes after foliar application of urea

Our research aimed to determine the effect and efficiency of foliar application of urea on the phenolic composition of Tempranillo Blanco grapes. The field experiment was carried out in 2019 and 2020 seasons and the plot was located in D.O.Ca Rioja (North of Spain). The vineyard was Vitis vinifera L. Tempranillo Blanco and grafted on Richter-110 rootstock. The treatments were control (C), whose plants were sprayed with water and three doses of urea: plants were sprayed with urea 3 kg N/ha (U3), 6 kg N/ha (U6) and 9 kg N/ha (U9). The applications were performed in two phenological stages, pre-veraison (Pre) and veraison (Ver). Also, each of the treatments was repeated one week later. Control and treatments were performed in triplicate and arranged in a randomised block design. Grapes were harvested at optimum ripening stage. High-performance liquid chromatography was used to analyse the phenolic composition of the grapes. Finally, the results obtained from the analytical determinations – flavonols, flavanols and non-flavonoid (hydroxybenzoic acids, hydroxycinnamic acids and stilbenes) – were studied statistically by analysis of variance. The results showed that, in 2019, U6-Pre and U9-Pre treatments increased the hydroxybenzoic acid content in grapes, and also all foliar treatments applied at Pre enhanced the stilbene concentration. Moreover, U3-Ver was the only treatment that rose flavonol and stilbene contents in the Tempranillo Blanco grapes. In 2020, all treatments applied at Pre enhanced the flavonol concentration in grapes. Furthermore, U3-Pre and U9-Pre treatments increased stilbene content in grapes. Nevertheless, the hydroxybenzoic acid content was improved by U6-Ver and U9-Ver and besides, hydroxycinnamic acid concentration in grapes was increased by all treatments applied at Ver. In conclusion, the lower and highest dose of urea (U3 and U9), applied at pre-veraison, were the best treatments to improve the Tempranillo Blanco grape phenolic composition.