Macrowine 2021
IVES 9 IVES Conference Series 9 Winemaking processes discrimination by using qNMR metabolomics

Winemaking processes discrimination by using qNMR metabolomics

Abstract

AIM: Metabolomics in food science has been increasingly used over the last twenty years. Among the tools used for wine, qNMR has emerged as a powerful tool to discern wines based on environmental factors such as geographical origin, grape variety and vintage (Gougeon et al., 2019a). Since human factors are less studied while they also contribute a lot to the wine making, we wondered if this technique could also dissociate physical or chemical processes used in oenology. The goal of this work is to allow a better understanding of the interactions between the oenological processes and wine by finding metabolites that are responsible of winemaking processes’s differentiations through 1H‑NMR metabolomics targeted and untargeted (fingerprinting) approaches combined with advanced chemiometrics.

METHODS: Wine analyses were realized by qNMR approaches. Targeted (based on nearly fifty wine constituents) and untargeted analyses were carried out on wines having undergone several physical and chemical processes. Principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and similarity score (S-score) (Gougeon et al., 2019b) were performed out for the analytical discrimination of winemaking processes.

RESULTS: qNMR analyses associated with chemometrics allow discriminating not only the physical processed such as the filtration but also chemical processes like the maceration temperature, enzyme treatment and fining agent effects. Furthermore, the impacted metabolites were highlighted providing valuable data on the winemaking processes investigated.

CONCLUSIONS:

qNMR metabolomics offers a fast and reliable method to study the effects of winemaking practices on wine quality.

DOI:

Publication date: September 10, 2021

Issue: Macrowine 2021

Type: Article

Authors

Inès Le Mao

University of Bordeaux, Œnology EA 4577, USC 1366 INRA, INP, ISVV, 210 chemin de Leysotte, 33882 Villenave d’Ornon, France,Gregory Da Costa, Jean Martin, Wiame El Batoul, Charlyne Bautista, Soizic Lacampagne, Tristan Richard University of Bordeaux, Œnology EA 4577, USC 1366 INRA, INP, ISVV, 210 chemin de Leysotte, 33882 Villenave d’Ornon, France

Contact the author

Keywords

metabolomics, qnmr, winemaking processes, quality

Citation

Related articles…

Sustaining wine identity through intra-varietal diversification

With contemporary climate change, cultivated Vitis vinifera L. is at risk as climate is a critical component in defining ecologically fitted plant materiel. While winegrowers can draw on the rich diversity among grapevine varieties to limit expected impacts (Morales-Castilla et al., 2020), replacing a signature variety that has created a sense of local distinctiveness may lead to several challenges. In order to sustain wine identity in uncertain climate outcomes, the study of intra-varietal diversity is important to reflect the adaptive and evolutionary potential of current cultivated varieties. The aim of this ongoing study is to understand to what extent can intra-varietal diversity be a climate change adaptation solution. With a focus on early (Sauvignon blanc, Riesling, Grolleau, Pinot noir) to moderate late (Chenin, Petit Verdot, Cabernet franc) ripening varieties, data was collected for flowering and veraison for the various studied accessions (from conservatory plots) and clones. For these phenological growing stages, heat requirements were established using nearby weather stations (adapted from the GFV model, Parker et al., 2013) and model performances were verified. Climate change projections were then integrated to predict the future behaviour of the intra-varietal diversity. Study findings highlight the strong phenotypic diversity of studied varieties and the importance of diversification to enhance climate change resilience. While model performances may require improvements, this study is the first step towards quantifying heat requirements of different clones and how they can provide adaptation solutions for winegrowers to sustain local wine identity in a global changing climate. As genetic diversity is an ongoing process through point mutations and epigenetic adaptations, perspective work is to explore clonal data from a wide variety of geographic locations.

Vine environment interaction as a method for land viticultural evaluation. An experience in Friuli Venezia Giulia (N-E of Italy)

For a long time environment was known as one of the most important factors to characterize the quality of wines but at the same time it appears very difficult to distinguish inside the “terroir” the role of the single factor. These remarks partially explain why methods for viticultural evaluation are often quite different (Amerine et al., 1944; Antoniazzi et al., 1986; Asselin et al., 1987; Astruc et al., 1980; Bonfils, 1977; Boselli, 1991; Colugnati, 1990; Costantinescu, 1967; Costantini et al., 1987; Dutt et al., 1981; Falcetti et al., 1992; Fregoni et al., 1992; Hidalgo, 1980; Intrieri et al., 1988; Laville, 1990; Morlat et al., 1991; Scienza et al., 1990; Shubert et al., 1987; Turri et al., 1991).

Settling precocity and growth kinetics of the primary leaf area: two indicative parameters of grapevine behaviour

Le comportement de la vigne en terme de fonctionnement thermique et hydrique, influe de manière directe sur la qualité des baies de raisin. L’effet du terroir peut être perçu à travers l’étude de paramètres tels que la précocité, la mise en place de la surface foliaire ou la vigueur. Une expérimentation a été conduite en Val de Loire sur le cépage chenin dans le but de mieux comprendre le rôle des variables liées au terroir sur la croissance et le développement de la vigne et in fine sur la qualité des baies.

Mobilizing endogenous transposable elements for grapevine improvement: a genomic and epigenomic approach in New Zealand Sauvignon Blanc

Efforts to improve the New Zealand wine industry’s climate resilience and sustainability through grapevine improvement are limited by germplasm availability and a reliance on Sauvignon Blanc exports. To address this, we are working to generate a population of 12,000 individuals with unique genetic traits, from which to select future clones for major export varieties.

Sauvignon Blanc plantlets are being regenerated from embryogenic callus, using an approach designed to mobilise endogenous transposable elements as mutagens.

Vineyard management strategies adopted to mitigate the impacts of climate change affect the evolution of phenolics and color during bottle aging of Aglianico wines

In recent years several strategies have been proposed to cope with the effect of climate change on grape berry quality but only a few studies have dealt with the influence of management practices implemented in the field (e.g. irrigation,summer pruning, etc.), on the evolution of wines over time.