Macrowine 2021
IVES 9 IVES Conference Series 9 Winemaking processes discrimination by using qNMR metabolomics

Winemaking processes discrimination by using qNMR metabolomics

Abstract

AIM: Metabolomics in food science has been increasingly used over the last twenty years. Among the tools used for wine, qNMR has emerged as a powerful tool to discern wines based on environmental factors such as geographical origin, grape variety and vintage (Gougeon et al., 2019a). Since human factors are less studied while they also contribute a lot to the wine making, we wondered if this technique could also dissociate physical or chemical processes used in oenology. The goal of this work is to allow a better understanding of the interactions between the oenological processes and wine by finding metabolites that are responsible of winemaking processes’s differentiations through 1H‑NMR metabolomics targeted and untargeted (fingerprinting) approaches combined with advanced chemiometrics.

METHODS: Wine analyses were realized by qNMR approaches. Targeted (based on nearly fifty wine constituents) and untargeted analyses were carried out on wines having undergone several physical and chemical processes. Principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and similarity score (S-score) (Gougeon et al., 2019b) were performed out for the analytical discrimination of winemaking processes.

RESULTS: qNMR analyses associated with chemometrics allow discriminating not only the physical processed such as the filtration but also chemical processes like the maceration temperature, enzyme treatment and fining agent effects. Furthermore, the impacted metabolites were highlighted providing valuable data on the winemaking processes investigated.

CONCLUSIONS:

qNMR metabolomics offers a fast and reliable method to study the effects of winemaking practices on wine quality.

DOI:

Publication date: September 10, 2021

Issue: Macrowine 2021

Type: Article

Authors

Inès Le Mao

University of Bordeaux, Œnology EA 4577, USC 1366 INRA, INP, ISVV, 210 chemin de Leysotte, 33882 Villenave d’Ornon, France,Gregory Da Costa, Jean Martin, Wiame El Batoul, Charlyne Bautista, Soizic Lacampagne, Tristan Richard University of Bordeaux, Œnology EA 4577, USC 1366 INRA, INP, ISVV, 210 chemin de Leysotte, 33882 Villenave d’Ornon, France

Contact the author

Keywords

metabolomics, qnmr, winemaking processes, quality

Citation

Related articles…

Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

2,5-Dimethyl-4-hydroxy-3(2H)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. Several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from American grape (Vitis labrusca) and its hybrid grape, similar to methyl anthranilate. Muscat Bailey A is a hybrid grape variety [V. labrusca (Bailey) x V. vinifera (Muscat Hamburg)], and its wine is one of the most popular in Japan. The inclusion of Muscat Bailey A in the ‘International List of Vine and Varieties and their Synonyms’ managed by the ‘International Organisation of Vine and Wine (OIV)’ in 2013 has further fueled its popularity among winemakers and researchers worldwide.

Improved analysis of isomeric polyphenol dimers using the 4th dimension of trapped ion mobility spectrometry – mass spectrometry

Dehydrodicatechins have recently received attention as oxidation markers especially in grapes and wine. Their analysis mainly uses LC-MS/MS which is able to differentiate them from their natural isomers (dimeric procyanidins), based on specific fragments

Cover crop management and termination timing have different effects on the maturation and water potentials of Glera (Vitis vinifera L.) in Friuli-Venezia Giulia

Inter-row soil tillage in vineyards, stimulates vigor and production due to the absence of competition for water and nutrients, however negatively affects organic matter content, soil erosion, and compaction, resulting in reduced fertility. In this study, we investigated the effects of different cover crop management approaches, including cultivation type and termination timing, on the physiological and productive responses of a Glera vineyard.
The experimental trial was conducted in Precenicco (UD) from 2019 to 2021. A commercial mixture for autumn cover cropping was sown in alternating rows, and the sowing pattern was changed each year.

Determination of selected phenolics, carotenoids and norisoprenoids in Riesling grapes after treatment against sunburn damage

Riesling represents the most widely cultivated grape variety in Germany and is therefore of particular economic interest. During recent years an increase in the petrol-note as well as in undesirable bitter and adstringent notes has been reported. These changes are most likely linked to increasing temperature and sunlight exposure of grapes due to climate changes.
The “petrol note” is caused by the formation of the C13-norisoprenoid 1,1,6-trimethyl-1,2-dihydronaphthalin (TDN), which originates from acid-labile precursors formed by the carotenoid degradation in the grape.

A few observations on double sigmoid fruit growth

Many fleshy fruit, including the grape berry, exhibit a double‐sigmoid growth (DSG) pattern. Identification of the curious DSG habit has long been attributed to Connors’ (1919) work with peaches