Macrowine 2021
IVES 9 IVES Conference Series 9 Winemaking processes discrimination by using qNMR metabolomics

Winemaking processes discrimination by using qNMR metabolomics

Abstract

AIM: Metabolomics in food science has been increasingly used over the last twenty years. Among the tools used for wine, qNMR has emerged as a powerful tool to discern wines based on environmental factors such as geographical origin, grape variety and vintage (Gougeon et al., 2019a). Since human factors are less studied while they also contribute a lot to the wine making, we wondered if this technique could also dissociate physical or chemical processes used in oenology. The goal of this work is to allow a better understanding of the interactions between the oenological processes and wine by finding metabolites that are responsible of winemaking processes’s differentiations through 1H‑NMR metabolomics targeted and untargeted (fingerprinting) approaches combined with advanced chemiometrics.

METHODS: Wine analyses were realized by qNMR approaches. Targeted (based on nearly fifty wine constituents) and untargeted analyses were carried out on wines having undergone several physical and chemical processes. Principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and similarity score (S-score) (Gougeon et al., 2019b) were performed out for the analytical discrimination of winemaking processes.

RESULTS: qNMR analyses associated with chemometrics allow discriminating not only the physical processed such as the filtration but also chemical processes like the maceration temperature, enzyme treatment and fining agent effects. Furthermore, the impacted metabolites were highlighted providing valuable data on the winemaking processes investigated.

CONCLUSIONS:

qNMR metabolomics offers a fast and reliable method to study the effects of winemaking practices on wine quality.

DOI:

Publication date: September 10, 2021

Issue: Macrowine 2021

Type: Article

Authors

Inès Le Mao

University of Bordeaux, Œnology EA 4577, USC 1366 INRA, INP, ISVV, 210 chemin de Leysotte, 33882 Villenave d’Ornon, France,Gregory Da Costa, Jean Martin, Wiame El Batoul, Charlyne Bautista, Soizic Lacampagne, Tristan Richard University of Bordeaux, Œnology EA 4577, USC 1366 INRA, INP, ISVV, 210 chemin de Leysotte, 33882 Villenave d’Ornon, France

Contact the author

Keywords

metabolomics, qnmr, winemaking processes, quality

Citation

Related articles…

Growers’ attitudes towards organic certification: the case of Central Otago, New Zealand

New Zealand viticulture has long been characterised by sustainable grape growing practices as promoted by Sustainable Winegrowing New Zealand (SWNZ) as well as by Organic Viticulture.

Correlation between skin cell wall composition and phenolic extractability in Cabernet sauvignon wines

The phenolic component of red wine is responsible for important elements of flavor and mouthfeel, and thus quality of the finished wine. Additionally, many of these phenolics have been associated with health benefits such as reduction of the risk of developing cardiovascular disease, cancer, osteoporosis and preventing Alzheimer’s disease. While the origins, concentrations, and chemistries of the phenolics in a finished red wine are well known, the fundamental mechanisms and kinetics of extraction of these phenolics from grape skins and seeds during red wine fermentation are poorly understood. This lack of knowledge regarding the extraction mechanisms of phenolics during red wine fermentation makes informed manipulations of the finished wine’s phenolic composition difficult.

Improving shelf life of viticulture-relevant biocontrol and biostimulant microbes using CITROFOL® AI as liquid carrier

Bacillus velezensis and Trichoderma harzianum are relevant microorganisms used in viticulture as biocontrol agents against pathogens of trunk (e.g. Phaeoacremonium minimum), leaves (e.g. Plasmopara viticola) or fruit (e.g. Botrytis cinerea), or as biostimulants, improving the resilience of plants against biotic or abiotic stressors through different direct and non-direct interactions.
In this biotechnological approach, formulation plays a crucial role. Controlling water activity in the product, thus stabilising microbial viability is key to ensuring effective application. We present the benefits of the citrate ester CITROFOL® AI (triethyl citrate) as a novel bio-based carrier liquid in microbial formulations. CITROFOL® AI is safe for humans and the environment, thus offering a promising base for sustainable treatments in viticulture.

The terroir of Pinot noir wine in the Willamette valley, Oregon – a broad analysis of vineyard soils, grape juice and wine chemistry

Wine-grapes in the Willamette Valley, Oregon, are grown on three major soil parent materials: volcanic, marine sediments, and loess/volcanic.

Elicitors application in two maturation stages of Vitis vinifera L. cv Monastrell: changes on the skin cell walls

AIM: In a recent study, it was determined that the mid-ripening period is the most suitable for the application of methyl jasmonate (MeJ), benzothiadiazole BTH and MeJ+BTH on Monastrell grapes, to favor maximum accumulation of phenolic compounds at the time of harvest. However, the increase in the anthocyanin content of