Macrowine 2021
IVES 9 IVES Conference Series 9 Inhibition of reductive characters in wine by cu-organic acids: predicting the duration of protection

Inhibition of reductive characters in wine by cu-organic acids: predicting the duration of protection

Abstract

Cu organic acid complexes efficiently bind hydrogen sulfide in wine and therefore prevent its accumulation and subsequent reductive off-flavour [1]. This fraction of Cu can also bind methanethiol, the other main contributor to reductive wine characters, albeit with less efficient binding than for hydrogen sulfide [1]. However, during bottle aging of wine, the concentration of Cu organic acid complexes gradually decline and the sulfide-bound form of Cu increases. The point at which the Cu organic acid concentration is depleted signifies a potential for reductive aroma development to occur. The aim of this study is ascertain how long Cu organic acid complexes in wine can offer a protection against the reductive aroma compounds. High (0.6 mg/L), medium (0.3 mg/L) and no (0 mg/L) Cu(II) additions were made to Pinot Grigio and Chardonnay wines at bottling, and the bottled wines were then stored at 14 °C in darkness. Analysis was performed on the wines at 0, 2, 4, 8 and 12-14 months after bottling. Throughout bottle ageing process, the concentrations of three different Cu fractions, attributed to Cu organic acid complexes, Cu thiol complexes and sulfide-bound Cu, were monitored by stripping potentiometry and colorimetric methods. The free and salt-releasable concentration of hydrogen sulfide and methanethiol were determined by gas chromatography with chemiluminescene detection. Sensorial analysis was also performed on the wines after 12 months. During the first 2-months of bottle aging of all wines, the Cu-organic acid concentrations initially remained stable or increased, as total packed oxygen was consumed. Afterwards, during the low oxygen aging phase of the wines, the Cu-organic acid concentration declined exponentially with a concomitant increase in sulfide-bound Cu. These changes in sulfide-bound Cu were matched by an increase the salt-releasable hydrogen sulfide concentrations of the wines during aging. Free concentrations of hydrogen sulfide and methanethiol were only found to accumulate in wines without any Cu-organic acid present at bottling. For the Pinot Grigio without Cu-organic acid present at bottling (i.e., the no Cu addition treatment), the free methanethiol concentrations in the wine were above the aroma threshold and this wine was assessed as reductive. Alternatively, for the Pinot Grigio with Cu-organic acid complexes at bottling, only concentrations of free methanethiol below the aroma threshold were measured and the wine was not reductive. The decay in Cu-organic acid complexes, in the low oxygen aging phase of the wines, were found to follow first order kinetics that were similar for the Chardonnay and the Pinot Grigio. These results enable determination of the time-frame that Cu-organic acids can offer white wine protection against the potential accumulation of reductive aroma compounds.

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Xinyi Zhang

National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, Australia,Nikolaos KONTOUDAKIS (Laboratory of Enology and Alcoholic Drinks, Department of Food Science and Human Nutrition, Agricultural University of Athens) John W. BLACKMAN (National Wine and Grape Industry Centre, Charles Sturt University) Andrew C. CLARK (National Wine and Grape Industry Centre, Charles Sturt University)

Contact the author

Keywords

cu organic acid complexes, hydrogen sulfide accumulation, white wine bottle ageing

Citation

Related articles…

A better understanding of the climate effect on anthocyanin accumulation in grapes using a machine learning approach

The current climate changes are directly threatening the balance of the vineyard at harvest time. The maturation period of the grapes is shifted to the middle of the summer, at a time when radiation and air temperature are at their maximum. In this context, the implementation of corrective practices becomes problematic. Unfortunately, our knowledge of the climate effect on the quality of different grape varieties remains very incomplete to guide these choices. During the Innovine project, original experiments were carried out on Syrah to study the combined effects of normal or high air temperature and varying degrees of exposure of the berries to the sun. Berries subjected to these different conditions were sampled and analyzed throughout the maturation period. Several quality characteristics were determined, including anthocyanin content. The objective of the experiments was to investigate which climatic determinants were most important for anthocyanin accumulation in the berries. Temperature and irradiance data, observed over time with a very thin discretization step, are called functional data in statistics. We developed the procedure SpiceFP (Sparse and Structured Procedure to Identify Combined Effects of Functional Predictors) to explain the variations of a scalar response variable (a grape berry quality variable for example) by two or three functional predictors (as temperature and irradiance) in a context of joint influence of these predictors. Particular attention was paid to the interpretability of the results. Analysis of the data using SpiceFP identified a negative impact of morning combinations of low irradiance (lower than about 100 μmol m−2 s−1 or 45 μmol m−2 s−1 depending on the advanced-delayed state of the berries) and high temperature (higher than 25oC). A slight difference associated with overnight temperature occurred between these effects identified in the morning.

Influence of successive oxygen saturations of a grape juice, supplemented or not with laccase, on its color and hydroxycinnamic acids concentration

Aim: This work studies how successive O2 saturations affects the color and hydroxycinnamic
acids concentration in the absence and presence of laccase from B. cinerea with the aim of better understanding the browning processes.

Materials and methods: Grapes of Muscat of Alexandria were harvested and pressed with a vertical press to extract 60% of their juice. Aliquots of 30 mL of this must were placed in 60 mL flasks equipped with a pill (PreSens Precision Sensing GmbH) for measuring oxygen by luminescence (Nomasense TM O2 Trace Oxygen Analyzer).

Tuning the pH during the fermentation has a strong effect on the wine protein composition and the stability of the resulting white wines

Previous results have shown the impact of the pH on the stability of white wine proteins. In a context of global warming that implies increases in ethanol content and pH

The combined effects of climate, soils, and deficit irrigation on yield and quality of Touriga Nacional under high atmospheric demand in the Douro Region

Global warming is one of the biggest environmental, social and economic threats in several viticultural regions. In the Douro Valley, changes are expected in the coming years, namely an increase in temperature and a decrease in precipitation. These changes are likely to have consequences for the production and quality of wine.
The aim of this study was to explore the effects of different soil characteristics combined with several deficit irrigation strategies, managed throughout ETc references and predawn leaf water potentials thresholds, on physiology, yield, and qualitative attributes on the Touriga Nacional variety under years of mild to severe water and heat stress.
The studies were conducted over seven years (2015 to 2021) in two plots of a commercial vineyard located at Quinta do Ataíde (Symington Family Estates) planted in 2011 and 2014 at 170 meters elevation, growing under three water regimes: non-irrigated (NI) and two deficit irrigation strategies (30% and 60% ETc) assessed weekly by Ψpd. The site has an annual rainfall below 500 mm, with high atmospheric demand. Climate data was collected from a weather station, located on site. Berry ripening was followed weekly for fruit analysis. At harvest, yield, vigour and pruning weight per vine were determined from 90 vines by treatment. Each season at veraison the NDVI Index was accessed by a drone. The soils physic-chemistry in the experimental blocs were analysed and grouped by SWHC. Delta C-13 analyses were also performed per treatment in two years.Irrigation had a positive effect on yield per vine, mostly due to an increase in berry and cluster weight, and fertility index through the years. A significant increase in sugar content, colour and phenols was observed with deficit irrigation in some years, but vine vigour related to soil characteristics had by far the greatest impact on quality.

Qualitative and productive characterization of a minority variety: ‘Branco lexítimo’ in DO Ribeira Sacra (Spain)

The actual climate changes, together with the strong regulation of the European Union and Spanish government, in search of sustainable viticulture, have forced the recovery of minority varieties, expanding the range of grape varieties, as well as the possible development of wines with unique profiles. In the Ribeira Sacra DO (Spain), a comparative study of the agronomic and qualitative behavior of the ‘Branco lexítimo’ variety has been carried out, compared to the majority white variety in the DO: ‘Godello’, located in the same study plot, with identic soil and climatic conditions. The study contemplated the analysis of phenology and leaf water potential, as well as the productive results and the analysis of the must quality, during four seasons: 2018 – 2021.