Macrowine 2021
IVES 9 IVES Conference Series 9 Inhibition of reductive characters in wine by cu-organic acids: predicting the duration of protection

Inhibition of reductive characters in wine by cu-organic acids: predicting the duration of protection

Abstract

Cu organic acid complexes efficiently bind hydrogen sulfide in wine and therefore prevent its accumulation and subsequent reductive off-flavour [1]. This fraction of Cu can also bind methanethiol, the other main contributor to reductive wine characters, albeit with less efficient binding than for hydrogen sulfide [1]. However, during bottle aging of wine, the concentration of Cu organic acid complexes gradually decline and the sulfide-bound form of Cu increases. The point at which the Cu organic acid concentration is depleted signifies a potential for reductive aroma development to occur. The aim of this study is ascertain how long Cu organic acid complexes in wine can offer a protection against the reductive aroma compounds. High (0.6 mg/L), medium (0.3 mg/L) and no (0 mg/L) Cu(II) additions were made to Pinot Grigio and Chardonnay wines at bottling, and the bottled wines were then stored at 14 °C in darkness. Analysis was performed on the wines at 0, 2, 4, 8 and 12-14 months after bottling. Throughout bottle ageing process, the concentrations of three different Cu fractions, attributed to Cu organic acid complexes, Cu thiol complexes and sulfide-bound Cu, were monitored by stripping potentiometry and colorimetric methods. The free and salt-releasable concentration of hydrogen sulfide and methanethiol were determined by gas chromatography with chemiluminescene detection. Sensorial analysis was also performed on the wines after 12 months. During the first 2-months of bottle aging of all wines, the Cu-organic acid concentrations initially remained stable or increased, as total packed oxygen was consumed. Afterwards, during the low oxygen aging phase of the wines, the Cu-organic acid concentration declined exponentially with a concomitant increase in sulfide-bound Cu. These changes in sulfide-bound Cu were matched by an increase the salt-releasable hydrogen sulfide concentrations of the wines during aging. Free concentrations of hydrogen sulfide and methanethiol were only found to accumulate in wines without any Cu-organic acid present at bottling. For the Pinot Grigio without Cu-organic acid present at bottling (i.e., the no Cu addition treatment), the free methanethiol concentrations in the wine were above the aroma threshold and this wine was assessed as reductive. Alternatively, for the Pinot Grigio with Cu-organic acid complexes at bottling, only concentrations of free methanethiol below the aroma threshold were measured and the wine was not reductive. The decay in Cu-organic acid complexes, in the low oxygen aging phase of the wines, were found to follow first order kinetics that were similar for the Chardonnay and the Pinot Grigio. These results enable determination of the time-frame that Cu-organic acids can offer white wine protection against the potential accumulation of reductive aroma compounds.

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Xinyi Zhang

National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, Australia,Nikolaos KONTOUDAKIS (Laboratory of Enology and Alcoholic Drinks, Department of Food Science and Human Nutrition, Agricultural University of Athens) John W. BLACKMAN (National Wine and Grape Industry Centre, Charles Sturt University) Andrew C. CLARK (National Wine and Grape Industry Centre, Charles Sturt University)

Contact the author

Keywords

cu organic acid complexes, hydrogen sulfide accumulation, white wine bottle ageing

Citation

Related articles…

Influence of harvest time and withering length combination on reinforced Nebbiolo wines: phenolic composition, colour traits, and sensory profile

Sforzato di Valtellina DOCG is a reinforced dry red wine produced in the mountain area of Valtellina alpine valley (North Italy), using ‘Nebbiolo’ grapes that undergo a withering process. This process impacts on the grape composition due to a sugar concentration and changes in secondary metabolism influencing volatile organic compounds (VOCs) and polyphenols.

A blueprint for managing vine physiological balance at different spatial and temporal scales in Champagne

In Champagne, the vine adaptation to different climatic and technical changes during these last 20 years can be seen through physiological balance disruptions. These disruptions emphasize the general grapevine decline. Since the 2000s, among other nitrogen stress indicators, the must nitrogen has been decreasing. The combination of restricted mineral fertilizers and herbicide use, the growing variability of spring rainfall, the increasing thermal stress as well as the soil type heterogeneity are only a few underlying factors that trigger loss of physiological balance in the vineyards. It is important to weigh and quantify the impact of these factors on the vine. In order to do so, the Comité Champagne uses two key-tools: networking and modelization. The use of quantitative and harmonized ecophysiological indicators is necessary, especially in large spatial scales such as the Champagne appellation. A working group with different professional structures of Champagne has been launched by the Comité Champagne in order to create a common ecophysiology protocol and thus monitor the vine physiology, yearly, around 100 plots, with various cultural practices and types of soil. The use of crop modelling to follow the vine physiological balance within different pedoclimatic conditions enables to understand the present balance but also predict the possible disruptions to come in future climatic scenarios. The physiological references created each year through the working group, benefit the calibration of the STICS model used in Champagne. In return, the model delivers ecophysiology indicators, on a daily scale and can be used on very different types of soils. This study will present the bottom-up method used to give accurate information on the impacts of soil, climate and cultural practices on vine physiology.

Ugni blanc berry and wine composition impacted by thirteen rootstocks

The Cognac region is expanding, driven by the success of its renowned brandy and the demand for high grape yields to ensure a steady supply of base wine for distillation. Ugni blanc, the most widely planted grape variety, relies on rootstocks for soil and climate adaptation, providing essential nutrient supplies to the scion. Understanding the impact of rootstocks on key berry components, such as sugars and nitrogen compounds, is crucial. These compounds serve as primary precursors for the production of fermentative aroma metabolites, which, in turn, act as quality indicators for eau-de-vie.

Optimization of in vitro establishment of grapevine varieties for fast micropropagation 

Micropropagation is an important alternative to conventional methods of plant propagation. The objective of this study was to optimize a protocol for in vitro micropropagation of selected grapevine hybrids (H19 and H20) that are included in our breeding program. For the sprouting initiation experiment, nodal cuttings with only one axillary bud from two hybrids were separated, disinfected, and cultivated in 50% Murashige Skoog nutrient medium (½ MS) and Woody Plant Medium (WPM), adding 4.4 µM benzyladenine (BA) in both mediums.

Soil Temperature and Climate Change: Implications for Mediterranean Vineyards 

More frequent and extreme temperatures and droughts pose challenges to the wine sector in Mediterranean Europe. Soil is crucial to sustain the equilibrium of ecosystems, economic growth and people’s prosperity worldwide. In viticulture, soils are a major component of the terroir and do influence vine’s growth, yield and berry composition. Soil temperature (ST) affects soil´s physical, chemical and biological processes and also crop growth. The impact of ST becomes even stronger when dealing with row crops such as grapevine, when considering the increased exposition to radiation. However, the impact of ST on crop performance remains poorly described, especially for extreme climatic conditions.