Macrowine 2021
IVES 9 IVES Conference Series 9 Impact of mannoproteins structural features on the colloid stability when facing different kinds of wine polyphenols

Impact of mannoproteins structural features on the colloid stability when facing different kinds of wine polyphenols

Abstract

The aim was to study the impact of structural features in the polysaccharide moiety of mannoproteins on their interaction with polyphenols and the formation of colloidal aggregates. To this end, mannoproteins fractions were extracted from four different yeast strains: a commercial enological strain (MP-com), the wild-type BY4742 strain (MP-WT) and its mutants ΔMnn4 (MP-Mnn4) and ΔMnn2 (MP-Mnn2). The Mnn4p and Mnn2p are responsible for mannosyl-phosphorylation and branching of the N-glycosylation backbone [1]. Enzymatic extraction was performed using a commercial Endo-beta-1,3-Glucanase of Trichoderma sp. (E-LAMSE, Megazym)[2]. Mannoprotein fractions were thoroughly characterized by composition of their polysaccharide and protein moieties, branching degree, net charge, molecular weight distribution, static and dynamic molecular parameters [3]. Their interactions with seed tannins and a pool of red wine polyphenols and the formation of colloidal aggregates were studied in model solutions at different polyphenol/mannoprotein ratios through Dynamic Light Scattering (DLS). Model solutions were followed during one month. The number and size distribution of colloidal aggregates was determined by Nanoparticle Tracking Analysis (NTA).The four Mannoprotein fractions had broad and high molecular weight distributions, as well as similar protein, polysaccharide mass % and amino acid composition. However, they showed different proportions of mannose and glucose and the structural characterization of the polysaccharide moiety confirmed the expected differences between MP-WT, MP-Mnn2, and MP-Mnn4. DLS and NTA experiments indicated a two-step interaction process between seed tannins and mannoproteins: an immediate formation of colloidal aggregates (150-300 nm), followed by a very progressive evolution related to a reversible aggregate flocculation. The number, dispersity and extent of flocculation were dependent on the tannin/MP ratio. So far, no notable differences were evidenced between the four MP fractions. With the polyphenol pool of red wine, neither DLS nor NTA experiments were able to evidence the formation of colloidal aggregates. This does not mean that interactions do not exist[4,5]. Although the mannoproteins used had different polysaccharide compositions, structures, and properties, no difference in terms of colloidal behavior when in solution with tannins or wine polyphenols was evidenced by the methods applied. Thus, neither the absence of mannosyl phosphate groups (MP-Mnn4) nor the absence of branching of the outer chains of the N-glycosylated carbohydrate structures (MP-Mnn2) seems to play a determining role in the colloidal behavior of mannoproteins in the presence of seed tannins or red wine polyphenols.

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Saul Assunção Bicca

UMR-SPO, University of Montpellier, INRAE, Montpellier Supagro, Montpellier, France,Thierry, DOCO, UMR-SPO, University of Montpellier, INRAE, Montpellier Supagro, Montpellier, France Céline, PONCET-LEGRAND, UMR-SPO, University of Montpellier, INRAE, Montpellier Supagro, Montpellier, France Pascale, WILLIAMS, UMR-SPO, University of Montpellier, INRAE, Montpellier Supagro, Montpellier, France Julie MEKOUE N’GUELA, UMR-SPO, University of Montpellier, INRAE, Montpellier Supagro, Montpellier, France & Lallemand SAS, Blagnac, France Aude VERNHET, UMR-SPO, University of Montpellier, INRAE, Montpellier Supagro, Montpellier, France

Contact the author

Keywords

mannoproteins, colloidal stability, wine interactions

Citation

Related articles…

Postveraison shoot trimming in Tannat and Merlot: preliminary results on yield components, plant balance and berry composition

There is currently a trend towards the production of wines with low alcohol content. To achieve this, grapes with low sugar content must be used. There are techniques at the vineyard level that can delay ripening and avoid excessive sugar accumulation without, a priori, affecting the final polyphenol content. Postveraison shoot trimming (PVST) is experimentally evaluated for these purposes, but its impact under Uruguayan climatic conditions with high interannual variability is not known. The aim of this work is to assess the PVST in Tannat and Merlot cultivars and their impact on yield components, plant balance and berry primary composition. In this study, two commercial vineyards of 10 years old Tannat and Merlot (grafted on SO4) at Canelones Department were selected. During the 2020-201 growing season, grapevines were submitted to PVST when grapes reached 15º Brix. In a randomized block, trimmed (T) and control (C) plants were evaluated with three repetitions each cultivar. Evaluation of the evolution of primary berry composition during ripening, measurement of yield components and plant balance were performed. For both cultivars, PVST did not affect yield components. Merlot reached 5.4 kg per plant and Tannat 7.1 kg, with not statistical significance between treatments. However, statistical differences were observed in terms of plant balance. In Merlot Ravaz Index reached a difference of 5.3 (12.0 in T and 6.7 in C) meanwhile Tannat reached 3.5 of statistical difference (13.7 in T and 10.2 in C). The tendency to imbalance for the treated plants had an impact on the final grape composition. Merlot grapes showed statistical difference in final total acidity (0.3 g of difference between treatments) while treatments impact final sugar content on Tannat grapes (10.0 g of difference between treatments). Further studies are needed to assess the impact of different canopy management techniques in our conditions.

Upscaling the integrated terroir zoning through digital soil mapping: a case study in the Designation of Origin Campo de Borja

homogeneous zones by intersecting several partial zonings of major factors that influence vineyard growth. Each of them follows specific process from their corresponding disciplines. Soil zoning specifically refers to a Soil Resource Inventory map that has traditionally been generated by conventional soil mapping methods. These methods have shortcomings in reaching fine cartographic and categorical details and involve significant expenses, which undermines their applicability. A new framework named Digital Soil Mapping has introduced quantitative models by statistical techniques to establish soil-landscape relationships and is able to provide intensive scale cartography.

In the present study, a microzoning at 1:10.000 scale is generated from an initial zoning, where the conventional soil map with polytaxic map units is replaced by a new one from digital techniques that disaggregates them. The comparison between the zonings considers a quantitative evaluation of capability for each Homogeneous Terroir Unit by means of the Viticultural Quality Index and its categorization based on its distribution by map. The spatial intersection of both maps gives rise to a confusion matrix in which the flows of class variations after the substitution are assessed.

The results show a five-fold increase in the number of Homogeneous Terroir Units identified and a larger differentiation among them, evidenced by a wider range in the capability index distribution. Both elements are accompanied by an increase in the detection of areas of higher potential within previously undervalued uniform zones.These features are a direct effect of the improvements brought by Digital Soil Mapping techniques and would verify the advantages of their implementation in the Integrated Terroir zoning. Eventually, such new highly detailed terroir units would benefit precision viticulture and sustainable management practices.

Phenological characterization of a wide range of Vitis Vinifera varieties

In order to study the impact of climate change on Bordeaux grape varieties and to assess the adaptation capacities of candidates to the grape varieties of this wine region to the new climatic conditions, an experimental block design composed of 52 grape varieties was set up in 2009 at the INRAE Bordeaux Aquitaine center. Among the many parameters studied, the three main phenological stages of the vine (budburst, flowering and veraison) have been closely monitored since 2012. Observations for each year, stage and variety were carried out on four independent replicates. Precocity indices have been calculated from the data obtained over the 2012-2021 period (Barbeau et al. 1998). This work allowed to group the phenological behaviour of the grapevine varieties, not only based on the timing of the subsequent developmental stages, but also on the overall precocity of the cycle and the total length of the cycle between budburst and veraison. Results regarding the variability observed among the different grape varieties for these phenological stages are presented as heat maps.

Soil, vine, climate change – what is observed – what is expected

To evaluate the current and future impact of climate change on Viticulture requires an integrated view on a complex interacting system within the soil-plant-atmospheric continuum under continuous change. Aside of the globally observed increase in temperature in basically all viticulture regions for at least four decades, we observe several clear trends at the regional level in the ratio of precipitation to potential evapotranspiration. Additionally the recently published 6th assessment report of the IPCC (The physical science basis) shows case-dependent further expected shifts in climate patterns which will have substantial impacts on the way we will conduct viticulture in the decades to come.
Looking beyond climate developments, we observe rising temperatures in the upper soil layers which will have an impact on the distribution of microbial populations, the decay rate of organic matter or the storage capacity for carbon, thus affecting the emission of greenhouse gases (GHGs) and the viscosity of water in the soil-plant pathway, altering the transport of water. If the upper soil layers dry out faster due to less rainfall and/or increased evapotranspiration driven by higher temperatures, the spectral reflection properties of bare soil change and the transport of latent heat into the fruiting zone is increased putting a higher temperature load on the fruit. Interactions between micro-organisms in the rhizosphere and the grapevine root system are poorly understood but respond to environmental factors (such as increased soil temperatures) and the plant material (rootstock for instance), respectively the cultivation system (for example bio-organic versus conventional). This adds to an extremely complex system to manage in terms of increased resilience, adaptation to and even mitigation of climate change. Nevertheless, taken as a whole, effects on the individual expressions of wines with a given origin, seem highly likely to become more apparent.

Effect of multi-level and multi-scale spectral data source on vineyard state assessment

Currently, the main goal of agriculture is to promote the resilience of agricultural systems in a sustainable way through the improvement of use efficiency of farm resources, increasing crop yield and quality under climate change conditions. This last is expected to drastically modify plant growth, with possible negative effects, especially in arid and semi-arid regions of Europe on the viticultural sector. In this context, the monitoring of spatial behavior of grapevine during the growing season represents an opportunity to improve the plant management, winegrowers’ incomes, and to preserve the environmental health, but it has additional costs for the farmer. Nowadays, UAS equipped with a VIS-NIR multispectral camera (blue, green, red, red-edge, and NIR) represents a good and relatively cheap solution to assess plant status spatial information (by means of a limited set of spectral vegetation indices), representing important support in precision agriculture management during the growing season. While differences between UAS-based multispectral imagery and point-based spectroscopy are well discussed in the literature, their impact on plant status estimation by vegetation indices is not completely investigated in depth. The aim of this study was to assess the performance level of UAS-based multispectral (5 bands across 450-800nm spectral region with a spatial resolution of 5cm) imagery, reconstructed high-resolution satellite (Sentinel-2A) multispectral imagery (13 bands across 400-2500 nm with spatial resolution of <2 m) through Convolutional Neural Network (CNN) approach, and point-based field spectroscopy (collecting 600 wavelengths across 400-1000 nm spectral region with a surface footprint of 1-2 cm) in a plant status estimation application, and then, using Bayesian regularization artificial neural network for leaf chlorophyll content (LCC) and plant water status (LWP) prediction. The test site is a Greco vineyard of southern Italy, where detailed and precise records on soil and atmosphere systems, in-vivo plant monitoring of eco-physiological parameters have been conducted.