Macrowine 2021
IVES 9 IVES Conference Series 9 Validation of a high-throughput method for the quantification of volatile carbonyl compounds in wine and its use in accelerated ageing experiments

Validation of a high-throughput method for the quantification of volatile carbonyl compounds in wine and its use in accelerated ageing experiments

Abstract

AIM: the aim of this study was the optimization and validation of a robust and comprehensive method for the determination of volatile carbonyl compounds (VCCs) in wines. The protocol was then applied to determine the evolution of VCCs in wines after accelerated ageing. VCCs are widely present in foods and beverages; their formation is due to chemical reactions and biological processes where oxygen plays a key role [1]. However, many of these are side transformations that highly affect the final aroma. The total package oxygen is usually negligible in bottled wines. However, that amount combined with temperature and light, can modify the oxidative status with a consequent loss in varietal aroma and an increase in off-flavors and defects [2]. At the same time, several carbonyls are related to pleasant scents so the winemaking of many oxidized wines like Madeira, Port, Vin Santo is tailored to emphasize their productions. We expect that a high-throughput method for the measure of the concentration of carbonyls could be added as a new quality control tool for the evaluation of a complete fermentation, correct winemaking style, and proper bottling and storage.

METHODS: Various white wines (cv. Gewürztraminer) and red wines (cv. Teroldego) were submitted to accelerated-ageing process. All bottles were opened under inert atmosphere inside a sealed hood and submitted to the accelerated-ageing procedure, according to Oliveira et. al. [3]. The extraction procedure was based on the protocol purposed by Moreira et. al. [4], upgraded with a fully automated sample preparation performed by a CTC-PAL3 autosampler. The sample was transferred from the 2 mL vial (kept at 5°C) to a 20 mL vial and then spiked with internal standard (IS) and derivatizing agent (PFBHA) solutions. After a 7 minutes derivatization at 45°C, the SPME extraction is performed at 40°C for 20 minutes. Finally, the fiber desorption takes place at 250°C for 4 min. GC-MS analysis was carried out using a TSQ Quantum XLS Ultra Triple Quadrupole GC-MS/MS using MRM acquisition. Calibration curves were acquired in matrix using a commercial white wine treated with activated carbon to remove odor active compounds. Acetone d6, 4-methyl-4-penten-2-one d10, Octanal d16 and 4-fluorobenzaldehyde were used as IS. As many as 56 VCCs were the analytes under investigation.

RESULTS: all compounds showed a good linearity spanning from approximately 0.1 to 50 µg/L (R2>0.99). Intra-day and 5 days repeatability showed an RSD

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Maurizio Piergiovanni

University of Trento, Centre Agriculture, Food, Environment (C3A), San Michele all’Adige, Italy,Silvia, CARLIN, Research and Innovation Centre, Food Quality and Nutrition Department, Fondazione Edmund Mach, San Michele all’Adige, Italy  Cesare, LOTTI, Research and Innovation Centre, Food Quality and Nutrition Department, Fondazione Edmund Mach, San Michele all’Adige, Italy.  Fulvio, MATTIVI, University of Trento, Centre Agriculture, Food, Environment (C3A), San Michele all’Adige, Italy.

Contact the author

Keywords

carbonyls, oxidation, ageing, accelerated ageing, solid-phase micro extraction, automatization, oxygen, off-flavors

Citation

Related articles…

Underpinning terroir with data: rethinking the zoning paradigm

Agriculture, natural resource management and the production and sale of products such as wine are increasingly data-driven activities. Thus, the use of remote and proximal crop and soil sensors to aid management decisions is becoming commonplace and ‘Agtech’ is proliferating commercially; mapping, underpinned by geographical information systems and complex methods of spatial analysis, is widely used. Likewise, the chemical and sensory analysis of wines draws on multivariate statistics; the efficient winery intake of grapes, subsequent production of wines and their delivery to markets relies on logistics; whilst the sales and marketing of wines is increasingly driven by artificial intelligence linked to the recorded purchasing behaviour of consumers. In brief, there is data everywhere!

Opinions will vary on whether these developments are a good thing. Those concerned with the ‘mystique’ of wine, or the historical aspects of terroir and its preservation, may find them confronting. In contrast, they offer an opportunity to those interested in the biophysical elements of terroir, and efforts aimed at better understanding how these impact on vineyard performance and the sensory attributes of resultant wines. At the previous Terroir Congress, we demonstrated the potential of analytical methods used at the within-vineyard scale in the development of Precision Viticulture, in contributing to a quantitative understanding of regional terroir. For this conference, we take this approach forward with examples from contrasting locations in both the northern and southern hemispheres. We show how, by focussing on the vineyards within winegrowing regions, as opposed to all of the land within those regions, we might move towards a more robust terroir zoning than one derived from a mixture of history, thematic mapping, heuristics and the whims of marketers. Aside from providing improved understanding by underpinning terroir with data, such methods should also promote improved management of the entire wine value chain.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.

How can historical cultivars mitigate the effects of climate change?

IFV, INRAe and the national network “Partenaires de la Sélection Vigne” representing 37 organizations from the different wine regions, have been working increasingly closely over the last 2 decades towards the preservation of the French varietal patrimony. There are approximately 600 patrimonial varieties according to INRAe and SupAgro Montpellier experts, including ancient cultivars (400) and intravarietal crossbreeds obtained since the 19th century. In the context of a drastic reduction in such varieties from the mid 1980’s in favor of mainstream varieties, it was essential to carry out an inventory of old vines and vineyards. INRAe Vassal collection plays a key role here as it holds the largest diversity available, along with a rich bibliography and herbariums, offering us the opportunity to document and double check the identity of a cultivar, consolidating the expertise of ampelographers. The work is carried out in several stages, from verifying the existence of a variety in a small region, through to rehabilitation. During this session, the authors present the process that leads to the official registration of a variety. After this, IFV selection center takes over to initiate the process of selection and propagation. A specific focus within regions such as the Alps, Champagne and the South-West will provide details of the full procedure. Bia, Bouysselet, Chardonnay rose, Mecle and the aptly named Tardif, are some of the cultivars that have followed this procedure. Furthermore, a recent regulation established by INAO on “varieties of interest for adaptation purposes” might boost uptake by growers. Since 2006, 36 historical cultivars have been registered. Most of these have been neglected in the past due to late maturity, lack of sugar and high titratable acidity at harvest time. Such characteristics are today considered as positive qualities, not only in mitigation of the effects of climate change, but also as an opportunity for restoring diversity…

Impact of yeast derivatives to increase the phenolic maturity and aroma intensity of wine

Using viticultural and enological techniques to increase aromatics in white wine is a prized yet challenging technique for commercial wine producers. Equally difficult are challenges encountered in hastening phenolic maturity and thereby increasing color intensity in red wines. The ability to alter organoleptic and visual properties of wines plays a decisive role in vintages in which grapes are not able to reach full maturity, which is seen increasingly more often as a result of climate change. A new, yeast-based product on the viticultural market may give the opportunity to increase sensory properties of finished wines. Manufacturer packaging claims these yeast derivatives intensify wine aromas of white grape varieties, as well as improve phenolic ripeness of red varieties, but the effects of this application have been little researched until now. The current study applied the yeast derivative, according to the manufacture’s instructions, to the leaves of both neutral and aromatic white wine varieties, as well as on structured red wine varieties. Chemical parameters and volatile aromatics were analyzed in grape musts and finished wines, and all wines were subjected to sensory analysis by a tasting panel. Collective results of all analyses showed that the application of the yeast derivative in the vineyard showed no effect across all varieties examined, and did not intensify white wine aromatics, nor improve phenolic ripeness and color intensity in red wine.

Soil quality in Beaujolais vineyard. Importance of pedology and cultural practices

A pedological study was carried out from 2009 to 2017 in Beaujolais vineyard, to improve physical and chemical knowledge of soils. It was completed in 2016 and 2017 by the current study, dealing with microbial aspects, in order to build a reference frame for improved advice in soil management. Microbial biomass was measured on representative plots of the six most common soil types identified in Beaujolais and, for each soil type, on plots with different levels of the main impacting parameters: total organic carbon, pH, cation exchange capacity, extractable copper. A total of 59 soil samples were collected. Confirming the results of various trials carried out in Beaujolais over the past 20 years, the results of the present study showed that the soils were still alive, but exhibited a large variability of biological parameters, which appeared dependant on both pedological and anthropic factors. Therefore, a good interpretation of biological parameters and advice for vine growers must rely on a pedologically-based referential with differentiated main driving factors. For example, the control of pH is of primary importance in granitic soils and in no way organic matter addition can improve soil quality if pH is too low. Conversely, in calcareous soils, biological parameters are more directly affected by direct or indirect (cover crops for example) inputs of organic matter. The use of biological parameters, such as microbial biomass, is of great potential value to improve advice on agro-viticultural practices (soil management, fertilization, liming, etc.), basis of a sustainable wine production on fragile soils.