Macrowine 2021
IVES 9 IVES Conference Series 9 Validation of a high-throughput method for the quantification of volatile carbonyl compounds in wine and its use in accelerated ageing experiments

Validation of a high-throughput method for the quantification of volatile carbonyl compounds in wine and its use in accelerated ageing experiments

Abstract

AIM: the aim of this study was the optimization and validation of a robust and comprehensive method for the determination of volatile carbonyl compounds (VCCs) in wines. The protocol was then applied to determine the evolution of VCCs in wines after accelerated ageing. VCCs are widely present in foods and beverages; their formation is due to chemical reactions and biological processes where oxygen plays a key role [1]. However, many of these are side transformations that highly affect the final aroma. The total package oxygen is usually negligible in bottled wines. However, that amount combined with temperature and light, can modify the oxidative status with a consequent loss in varietal aroma and an increase in off-flavors and defects [2]. At the same time, several carbonyls are related to pleasant scents so the winemaking of many oxidized wines like Madeira, Port, Vin Santo is tailored to emphasize their productions. We expect that a high-throughput method for the measure of the concentration of carbonyls could be added as a new quality control tool for the evaluation of a complete fermentation, correct winemaking style, and proper bottling and storage.

METHODS: Various white wines (cv. Gewürztraminer) and red wines (cv. Teroldego) were submitted to accelerated-ageing process. All bottles were opened under inert atmosphere inside a sealed hood and submitted to the accelerated-ageing procedure, according to Oliveira et. al. [3]. The extraction procedure was based on the protocol purposed by Moreira et. al. [4], upgraded with a fully automated sample preparation performed by a CTC-PAL3 autosampler. The sample was transferred from the 2 mL vial (kept at 5°C) to a 20 mL vial and then spiked with internal standard (IS) and derivatizing agent (PFBHA) solutions. After a 7 minutes derivatization at 45°C, the SPME extraction is performed at 40°C for 20 minutes. Finally, the fiber desorption takes place at 250°C for 4 min. GC-MS analysis was carried out using a TSQ Quantum XLS Ultra Triple Quadrupole GC-MS/MS using MRM acquisition. Calibration curves were acquired in matrix using a commercial white wine treated with activated carbon to remove odor active compounds. Acetone d6, 4-methyl-4-penten-2-one d10, Octanal d16 and 4-fluorobenzaldehyde were used as IS. As many as 56 VCCs were the analytes under investigation.

RESULTS: all compounds showed a good linearity spanning from approximately 0.1 to 50 µg/L (R2>0.99). Intra-day and 5 days repeatability showed an RSD

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Maurizio Piergiovanni

University of Trento, Centre Agriculture, Food, Environment (C3A), San Michele all’Adige, Italy,Silvia, CARLIN, Research and Innovation Centre, Food Quality and Nutrition Department, Fondazione Edmund Mach, San Michele all’Adige, Italy  Cesare, LOTTI, Research and Innovation Centre, Food Quality and Nutrition Department, Fondazione Edmund Mach, San Michele all’Adige, Italy.  Fulvio, MATTIVI, University of Trento, Centre Agriculture, Food, Environment (C3A), San Michele all’Adige, Italy.

Contact the author

Keywords

carbonyls, oxidation, ageing, accelerated ageing, solid-phase micro extraction, automatization, oxygen, off-flavors

Citation

Related articles…

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares

Measurement of redox potential as a new analytical winegrowing tool

Excell laboratory has initiated the development of an analytical method based on electrochemistry to evaluate the ability of wines to undergo or resist to oxidative phenomena. Electrochemistry is a powerful tool to probe reactions involving electron transfers and offers possibility of real-time measurements. In that context, the laboratory has implemented electrochemical analysis to assess oxidation state of different wine matrices but also in order to evaluate oxidative or reduced character of leaf and soil. Initially, our laboratory focused on dosage of compounds involved in responses of plant stresses and we were also interested in microbiological activity of soils. These analyses were compared with the measurement of redox potential (Eh) and pH which are two fundamental variables involved in the modulation of plant metabolism. Indeed, the variation of redox states of the plant reflects its biological activity but also its capacity to absorb nutriments. The Eh-pH conditions mainly determine metabolic processes involved in soil and leaf and our goal is to determine if this combined analytical approach will be sufficiently precise to detect biological evolutions (plant health, parasitic attack…).

Adapting the vineyard to climate change in warm climate regions with cultural practices

Since the 1980s global regime shift, grape growers have been steadily adapting to a changing climate. These adaptations have preserved the region-climate-cultivar rapports that have established the global trade of wine with lucrative economic benefits since the middle of 17th century. The advent of using fractions of crop and actual evapotranspiration replacement in vineyards with the use of supplemental irrigation has furthered the adaptation of wine grape cultivation. The shift in trellis systems, as well as pruning methods from positioned shoot systems to sprawling canopies, as well as adapting the bearing surface from head-trained, cane-pruned to cordon-trained, spur-pruned systems have also aided in the adaptation of grapevine to warmer temperatures. In warm climates, the use of shade cloth or over-head shade films not only have aided in arresting the damage of heat waves, but also identified opportunities to reduce the evapotranspiration from vineyards, reducing environmental footprint of vineyard. Our increase in knowledge on how best to understand the response of grapevine to climate change was aided with the identification of solar radiation exposure biomarker that is now used for phenotyping cultivars in their adaptability to harsh environments. Using fruit-based metrics such as sugar-flavonoid relationships were shown to be better indicators of losses in berry integrity associated with a warming climate, rather than solely focusing on region-climate-cultivar rapports. The resilience of wine grape was further enhanced by exploitation of rootstock × scion combinations that can resist untoward droughts and warm temperatures by making more resilient grapevine combinations. Our understanding of soil-plant-atmosphere continuum in the vineyard has increased within the last 50 years in such a manner that growers are able to use no-till systems with the aid of arbuscular mycorrhiza fungi inoculation with permanent cover cropping making the vineyard more resilient to droughts and heat waves. In premium wine grape regions viticulture has successfully adapted to a rapidly changing climate thus far, but berry based metrics are raising a concern that we may be approaching a tipping point.

Towards adaptation to climate change in Rioja: Quality evaluation of wines obtained from Grenache x Tempranillo selections

The wine sector is of great relevance and tradition in Mediterranean countries, however, it may be most susceptible to climate change. In recent years, wine production is facing changes worldwide, both at environmental as well as commercial levels, due to global warming and the shift in consumers’ preferences. Wine growers and wine makers are in search of solutions that allow to face these new challenges. One of the most promising initiatives in the long term is the introduction of new plant materials, specifically intraspecific hybridizations between premium varieties that may improve traditional germplasm in its adaptation to climate change. These inter-varietal crosses have the potential to generate quality wines, whilst maintaining the regional typicity, and constitute an attractive alternative for the consumer due to their sensory attributes. In this study, we have evaluated wines from 29 intraspecific Garnacha x Tempranillo hybrids in two different locations, with the aim to assess their oenological potential and sensory attributes. Thirteen of the selections were white and 16 were red. Microvinifications were conducted with two or three replications depending on grape availability. Conventional oenological parameters were determined for all wines. The sensory evaluation and hedonic scores were given by five experts. Red selections obtained higher quality scores than white ones. Among the white selections with higher quality scores, GT-41 Varea and GT-159 Varea outstand, due to their high total acidity and high malic acid content. Regarding red selections, GT-57 Varea and GT-57 UR were perceived as higher in quality, highlighted for their moderate alcoholic and high anthocyanin content. Our results indicate that intraspecific hybridization may be a powerful tool for adapting traditional cultivars to climate change in Rioja.

Low-cost sensors as a support tool to monitor soil-plant heat exchanges in a Mediterranean vineyard

Mediterranean viticulture is increasingly exposed to more frequent extreme conditions such as heat waves. These extreme events co-occur with low soil water content, high air vapor pressure deficit and high solar radiant energy fluxes and result in leaf and berry sunburn, lower yield, and berry quality, which is a major constraint for the sustainability of the sector. Grape growers must find ways to proper and effectively manage heat waves and extreme canopy and berry temperatures. Irrigation to keep soil moisture levels and enable adequate plant turgor, and convective and evaporative cooling emerged as a key tool to overcome this major challenge. The effects of irrigation on soil and plant water status are easily quantifiable but the impact of irrigation on soil and canopy temperature and on heat convection from soil to cluster zone remain less characterized. Therefore, a more detailed quantification of vineyard heat fluxes is highly relevant to better understand and implement strategies to limit the effects of extreme weather events on grapevine leaf and berry physiology and vineyards performance. Low-cost sensor technologies emerge as an opportunity to improve monitoring and support decision making in viticulture. However, validation of low-cost sensors is mandatory for practical applicability. A two-year study was carried in a vineyard in Alentejo, south of Portugal, using low-cost thermal cameras (FLIR One, 80×60 pixels and FLIR C5, 160×120 pixels, 8-14 µm, FLIR systems, USA) and pocket thermohygrometers (Extech RHT30, EXTECH instruments, USA) to monitor grapevine and soil temperatures. Preliminary results show that low-cost cameras can detect severe water stress and support the evaluation of vertical canopy temperature variability, providing information on soil surface temperature. All these thermal parameters can be relevant for soil and crop management and be used in decision support systems.