Macrowine 2021
IVES 9 IVES Conference Series 9 Validation of a high-throughput method for the quantification of volatile carbonyl compounds in wine and its use in accelerated ageing experiments

Validation of a high-throughput method for the quantification of volatile carbonyl compounds in wine and its use in accelerated ageing experiments

Abstract

AIM: the aim of this study was the optimization and validation of a robust and comprehensive method for the determination of volatile carbonyl compounds (VCCs) in wines. The protocol was then applied to determine the evolution of VCCs in wines after accelerated ageing. VCCs are widely present in foods and beverages; their formation is due to chemical reactions and biological processes where oxygen plays a key role [1]. However, many of these are side transformations that highly affect the final aroma. The total package oxygen is usually negligible in bottled wines. However, that amount combined with temperature and light, can modify the oxidative status with a consequent loss in varietal aroma and an increase in off-flavors and defects [2]. At the same time, several carbonyls are related to pleasant scents so the winemaking of many oxidized wines like Madeira, Port, Vin Santo is tailored to emphasize their productions. We expect that a high-throughput method for the measure of the concentration of carbonyls could be added as a new quality control tool for the evaluation of a complete fermentation, correct winemaking style, and proper bottling and storage.

METHODS: Various white wines (cv. Gewürztraminer) and red wines (cv. Teroldego) were submitted to accelerated-ageing process. All bottles were opened under inert atmosphere inside a sealed hood and submitted to the accelerated-ageing procedure, according to Oliveira et. al. [3]. The extraction procedure was based on the protocol purposed by Moreira et. al. [4], upgraded with a fully automated sample preparation performed by a CTC-PAL3 autosampler. The sample was transferred from the 2 mL vial (kept at 5°C) to a 20 mL vial and then spiked with internal standard (IS) and derivatizing agent (PFBHA) solutions. After a 7 minutes derivatization at 45°C, the SPME extraction is performed at 40°C for 20 minutes. Finally, the fiber desorption takes place at 250°C for 4 min. GC-MS analysis was carried out using a TSQ Quantum XLS Ultra Triple Quadrupole GC-MS/MS using MRM acquisition. Calibration curves were acquired in matrix using a commercial white wine treated with activated carbon to remove odor active compounds. Acetone d6, 4-methyl-4-penten-2-one d10, Octanal d16 and 4-fluorobenzaldehyde were used as IS. As many as 56 VCCs were the analytes under investigation.

RESULTS: all compounds showed a good linearity spanning from approximately 0.1 to 50 µg/L (R2>0.99). Intra-day and 5 days repeatability showed an RSD

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Maurizio Piergiovanni

University of Trento, Centre Agriculture, Food, Environment (C3A), San Michele all’Adige, Italy,Silvia, CARLIN, Research and Innovation Centre, Food Quality and Nutrition Department, Fondazione Edmund Mach, San Michele all’Adige, Italy  Cesare, LOTTI, Research and Innovation Centre, Food Quality and Nutrition Department, Fondazione Edmund Mach, San Michele all’Adige, Italy.  Fulvio, MATTIVI, University of Trento, Centre Agriculture, Food, Environment (C3A), San Michele all’Adige, Italy.

Contact the author

Keywords

carbonyls, oxidation, ageing, accelerated ageing, solid-phase micro extraction, automatization, oxygen, off-flavors

Citation

Related articles…

EFFECT OF DIFFERENT TEMPERATURE AND WATER-LOSS DEHYDRATION CONDITIONS ON THE PATTERN OF FREE AND GLYCOSYLATED VOLATILE METABOLITES OF ITALIAN RED GRAPES

Post-harvest grape berries dehydration/withering are worldwide applied to produce high-quality sweet and dry wines (e.i., Vin Santo, Tokaji, Amarone della Valpolicella). Temperature and water loss impact grape metabolism [1] and are key variables in modulating the production of grape compounds of oenological interest, such as Volatile Organic Compounds (VOCs), secondary metabolites responsible for the aroma of the final wine.
The aim of this research was to assess the impact of post-harvest dehydration on free and glycosylated VOCs of two Italian red wine grapes, namely Nebbiolo and Aleatico, dehydrated in tunnel under controlled condition (varied temperature and weight-loss, at constant humidity and air flow). From these grapes Sforzato di Valtellina Passito DOCG and Elba Aleatico Passito DOCG, respectively.

Les AOC : un frein ou un moteur à l’innovation ? Retour sur l’histoire économique, scientifique, sociale et technique des vins d’appellation au XXe siècle en France

At a time when the world’s winegrowing industry is having to adapt to a number of challenges, winegrowers are wondering about the consequent changes they will have to make (grape varieties, changes in vineyard and cellar techniques). For winegrowers and consumers alike, there is also the question of how these changes will affect the taste of their wines. This research, based on the study of numerous sources and archives from the 20th century, some of which have never been published before, aims to show that, in the recent past, the winegrowing world has shown incredible resilience in the face of crises, and that the taste and perception of fine wines has changed considerably in 100 years.

Raman spectroscopy as a rapid method to assess grape polyphenolic maturation and wine malolactic fermentation on site

Wineries can increase their economic and environmental sustainability by optimizing the winemaking procedures, from harvest to wine maturation and conservation. Based on analytical data of the chemical composition and wine sensory evaluation, the enologist makes his own decision regarding the enological interventions at the harvest date selection, winemaking and post-winemaking.

An innovative 21st century frost alert system for an age-old viticulture challenge

Damage during the budbreak period due to spring season frosts remains one of the most significant weather-related challenges to viticulture around the world. For example, in 2021, €2bn of estimated damage was reported in france while >50% of vineyards were badly affected in the UK in 2017.

PHENOTYPIC DIVERSITY AND BIO-PROTECTION CAPABILITY OF METSCHNIKOWIA SP. IN OENOLOGY

Nowadays, the trend is to reduce the use of chemical inputs in the food sector, including in oenology. One of the inputs widely used in the wine making process are sulfites, for its several properties: antimicrobial and antioxidiant. This use isn’t without consequences on consumer’s health and environment, it can lead for example to allergic reactions and pollution. To limit the addition of chemical inputs, microbial alternatives are used. It consists to inoculate in grape must, a micro-organism able to inhibit the growth of the negative indigenous flora during the phase before the fermentation and to guarantee the sensory qualities of wines.