Macrowine 2021
IVES 9 IVES Conference Series 9 LC-MS based metabolomics discriminates premium from varietal chilean Cabernet Sauvignon cv. Wines

LC-MS based metabolomics discriminates premium from varietal chilean Cabernet Sauvignon cv. Wines

Abstract

Aim of the study was to investigate the metabolomic differences between Chilean Cabernet Sauvignon wines, divided according to their quality in two main groups: “Varietal” and “Premium”, and to point out metabolites tentative markers of their chemical signature and sensorial quality. Initially, 150 (50 x 3 biological replicates) experimental wines were produced by the same semi-industrial process, which covered 8 different Chilean valleys. The wine classification made by experts, divided the wines into two major groups (“Varietal” and “Premium”) and four subgroups (two for each major group). All the samples were analyzed according to a robust LC-MS based untargeted work-flow (Arapitsas et al 2018), and the proposed minimum reporting standards for chemical analysis of the Metabolomics Standards Initiative (Sumner et al 2007). The produced big-dataset was based on 7633 features for ESI- mode analysis and 9258 for ESI+ mode. After the quality control of the data, were found 150 tentative markers. The annotation of the markers was achieved by using the internal library, external databases and literature information. Between the Chilean Cabernet Sauvignon quality tentative markers were annotated N-compounds (e.g. peptides), stilbenoids, flavanols, anthocyanins and sulfonated compounds. In conclusion, this study allowed us to find metabolomic pathways and chemical reactions, and therefore propose new hypothesis, which could open new frontiers for the understanding of the Chilean Cabernet Sauvignon quality and terroir effect and provide new tools for the enology of precision in Chile.

DOI:

Publication date: September 24, 2021

Issue: Macrowine 2021

Type: Article

Authors

Vania Sáez,  Álvaro González, Panagiotis Arapitsas. 

Fondazione Edmund Mach.,Doreen Schober. Center for Research, and Innovation, Chile.  Fondazione Edmund Mach, Italy.

Contact the author

Keywords

cabernet sauvignon; quality; metabolomics; stilbenoids; anthocyanins; peptides; terroir

Citation

Related articles…

Effect of the addition of polysaccharides extracted for grape pomace and must on sensory and chemical composition of white wines

AIM: The objective of this work is to study the effect of the addition of polysaccharides extracted for grape pomace by-products and musts on sensory and chemical composition of white wines. Much of the waste obtained in the wine sector is not used, and they can have some valuable compounds, such as the polysaccharides (PS).

Recommended grapevine varieties for the vineyards zone Vrsac and trend meteorological elements

The aim of this paper was to analyze trends of the meteorological elements and determine suitability of growing grapevine cultivar in viticulture region.

A survey on the rotundone content of 18 grape varieties sourced from a germplasm 

Rotundone, the pepper aroma compound, has been detected in wines made from a large number of grape varieties. However, given the fact that analyzed wines were sourced from different winegrowing regions and seasons, made using different winemaking techniques and at different scales, it remains difficult to assess the real variety potential to produce rotundone.

The impact of sustainable management regimes on amino acid profiles in grape juice, grape skin flavonoids, and hydroxycinnamic acids

One of the biggest challenges of agriculture today is maintaining food safety and food quality while providing ecosystem services such as biodiversity conservation, pest and disease control, ensuring water quality and supply, and climate regulation. Organic farming was shown to promote biodiversity and carbon sequestration, and is therefore seen as one possibility of environmentally friendly production. Consumers expect organically grown crops to be free from chemical pesticides and mineral fertilizers and often presume that the quality of organically grown crops is different or higher compared to conventionally grown crops. Integrated, organic, and biodynamic viticulture were compared in a replicated field trial in Geisenheim, Germany (Vitis vinifera L. cv. Riesling). Amino acid profiles in juice, grape skin flavonoids, and hydroxycinnamic acids were monitored over three consecutive seasons beginning 7 years after conversion to organic and biodynamic viticulture, respectively. In addition, parameters such as soil nutrient status, yield, vigor, canopy temperature, and water stress were monitored to draw conclusions on reasons for the observed changes. Results revealed that the different sustainable management regimes highly differed in their amino acid profiles in juice and also in their skin flavonol content, whereas differences in the flavanol and hydroxycinnamic acid content were less pronounced. It is very likely that differences in nutrient status and yield determined amino acid profiles in juice, although all three systems showed similar amounts of mineralized nitrogen in the soil. Canopy structure and temperature in the bunch zone did not differ among treatments and therefore cannot account for the observed differences in favonols. A different light exposure of the bunches in the respective systems due to differences in vigor together with differences in berry size and a different water status of the vines might rather be responsible for the increase in flavonol content under organic and biodynamic viticulture.

The tolerance of grapevine rootstocks to water deficit is related to root morphology and xylem anatomy traits 

Climate change is altering water balances, thereby compromising water availability for crops. In grapevine, the strategic selection of genotypes more tolerant to soil water deficit can improve the resilience of the vineyard under this scenario. Previous studies demonstrated that root anatomical and morphological traits determine vine performance under water deficit conditions. Therefore, 13 ungrafted rootstock genotypes, 6 commercial (420 A, 41 B, Evex 13-5, Fercal, 140 Ru y 110 R), and 7 from new breeding programs (RG2, RG3, RG4, RG7, RG8, RG9 and RM2) were evaluated in pots during 2021 and 2022.