Impact of water stress on the phenolic composition of cv. Merlot grapes, in a typical terroir of the La Mancha region (Spain)

It is well known that high oxygen levels and high ageing temperatures are detrimental to white wine’s composition and ageing capacity. However, these results, though valuable

Conventional molecular analyses provide bulk genomic/transcriptomic data that are unable to reveal the cellular heterogeneity and to precisely define how gene networks orchestrate organ development. We will profile gene expression and identify open chromatin regions at the individual cells level, allowing to define cell-type specific regulatory elements, developmental trajectories and transcriptional networks orchestrating organ development and function. We will perform scRNA-seq and snATAC-seq on leaf/berry protoplasts and nuclei and combine them with the leaf/berry bulk tissues obtained results, where the analysis of transcripts, chromatin accessibility, histone modification and transcription factor binding sites showed that a large fraction of phenotypic variation appears to be determined by regulatory rather than coding variation and that many variants have an organ-specific effect.

Since the approval in 2019 of the use of high-power ultrasound (US) in winemaking to support extractive processes from grape to must, the study of this technology in red winemaking has increased significantly, with laboratory and semi-industrial scale studies.

Vigor declines in older vineyards and poor vine establishment in replant situations have been attributed to plant-parasitic nematodes. The northern root-knot nematode, Meloidogyne hapla, is the most prevalent plant-parasitic nematode species found in Washington wine grape vineyards. Management for nematodes in established vineyards is limited to the application of post-plant nematicides. We are evaluating new nematicides that are currently not registered in grape for their efficacy in controlling M. hapla and a part of that evaluation includes improving the alignment of nematicide application timing with the vulnerable second-stage juvenile (J2) life stage of M. hapla.

Sustainable viticulture and winemaking continue to represent huge challenges, where a better knowledge about the functional role of biodiversity in the vineyard ecosystems is required.