Estimation of plant hydraulics of grapevine in various «terroirs» in the Canton of Vaud (Switzerland)

In California vineyards, irrigation is considered as one of the most important decisions growers will make. Recent research has revealed that decisions of when to begin irrigation and how much water to apply have considerable consequences for final grape quality and hence wine quality. However, it is unclear whether and to what extent the average winegrape grower uses objective data to begin irrigating or to determine the amount of water to apply.

Developments in high-throughput sequencing (HTS) technologies allow us to obtain large amounts of microbial information from wine and must samples. Thus approaches, that are aimed at characterising the microbial diversity during fermentation, can be enhanced, or possibly even replaced, with HTS-based metabarcoding. To reduce experimental biases and increase data reproducibility, we compared 3 DNA extraction methods by evaluating differences in the fungal diversity with Riesling alcoholic fermentation samples at four different vineyards. The fungal diversity profiling was done using the genetic markers ITS2 and D2 using metabarcoding. The extraction methods compared consisted of a commercial kit, a recently published protocol that includes a DNA enhancer, and a protocol based on a buffer containing common inhibitor removal reagents. All methods were able to distinguish vineyard effects on the fungal diversity, but the results differed quantitatively.

The jurist feels like an intruder when talking about terroirs. He looks at the press and tries to understand. We can read there about the cooking festival of May 30, 1996 which “..highlights products whose quality depends on a region”, that Camembert du pays d’Auge is the only one to be protected, I was thinking of camembert from Normandy, that 80% of Greek feta is made in the Netherlands, I thought it was in Denmark, and that the European Community protects geographical indications of IGP origin, probably a new category replacing the indications protected areas (1). I also learned that distributors are asking for more local products because “they come to confuse the cards in the part engaged with the big brands”. Carrefour has its “Terroirs and drawers”, Prisunic its “Vent d’Ouest”, Intermarché “Les bouquets du terroir”, Monoprix “Les terroirs de France” (2), Promodés and its brand “Reflets de France” for the “Continent” hypermarkets (3). At the same time it is asserted that “The term is a mere common noun. Unprotectable and therefore unprotected” (4).

Brettanomyces bruxellensis is an ubiquitous yeast associated with different fermentation media such as beer and kombucha, where its presence is beneficial to bring an aromatic typicity. However, it is a main spoilage yeast in wines, in which it produces volatile phenols responsible for organoleptic deviations causing significant economic losses (Chatonnet et al., 1992). Cellar and winery equipment’s are considered as the first source of contamination, during fermentation and wine ageing process (Connel et al., 2002). Indeed, it is possible to find B. bruxellensis in the air, on walls and floors of the cellars, on small materials, vats and barrels.

The use of varieties tolerant to diseases is a long-term but promising option to reduce chemical input in viticulture. Several important breeding programs in Europe and abroad are starting to release a range of new hybrids performing well regarding fungi susceptibility and wine quality.