Characterization of spatial and temporal soil water status in vineyard by DC resistivity measurements

Over the last years, due to climate change, several red wines, such as the Sangiovese wines, have been often subjected to loss of clarity due to the formation of deposits of fine needle-shaped crystals. This phenomenon turned out to be due to an excess of quercetin (Q) and its glycosides (Q-Gs) in wines. These compounds are synthesized to a large extent when grapes are excessively exposed to UVB radiations in vineyards[1]. Unfortunately, it is not easy to predict the degree of Q precipitation because its solubility strongly depends on the wine and matrix composition[2].

Une unité de terroir naturel (UTN) peut être définie comme une unité de terre qui est caractérisée par une relative homogénéité topographique, climatique, géologique et pédologique. De telles unités sont de grande valeur pour mieux comprendre le système terroir/vigne/vin. Le but de cette étude est de caractériser la région viticole du Bottelaryberg. – Simonsberg-Helderberg en utilisant une information digitale existante et d’identifier des UTN en utilisant un Système d’information Géographique.

Global climate change affects regional climates and hold implications for wine growing regions worldwide

Happens too often in scientific papers to find the same harvesting period of a cultivar, although the used treatment influence a maturity curve of investigated thesis.
This inevitably leads to wrong conclusions when comparing the treatment effects, since obtained on maturity stages more or less far from those technologically correct.

Cis-acting regulatory elements are DNA sequences that can be bound by transcription factors to regulate the expression of genes in a condition-dependent and tissue-specific way. It is nowadays possible to search for DNA motives and sequences that a given transcription factor is binding or at least can, but it is still hard to have a glance at all the transcription factors that are contemporaneously located at the same locus. Inspired by an existing technique that uses the CRISPR-Cas system in mammal cells, we are trying to develop a protocol to study such regulation in Vitis vinifera. Using the highly sequence-specific binding capacity of a catalytically inactive Cas9 protein (dCas9), our idea is to set up a system to target a desired sequence and precipitate all the crosslinked proteins and distantly interacting chromatin at this locus and analyze them.