Terroir 2004 banner
IVES 9 IVES Conference Series 9 Analytical and Chemometric Investigation of Phenolic Content of South African Red Wines

Analytical and Chemometric Investigation of Phenolic Content of South African Red Wines

Abstract

Phenolic compounds have been the focus of a lot of research in recent years for their important contribution to sensory characteristics of wine, their beneficial health effects, as well as the possibility they offer of characterising wines. In this contribution, a method is developed that allows the direct injection of wine samples followed by liquid chromatography-diode array-electrospray ionisation-ion trap mass spectroscopy (MS) for the quantitative and qualitative analysis of a wide range of non-coloured phenolics. Diode array detection was found to be more suitable for quantitative purposes, while on-line UV spectra in combination with mass spectra greatly facilitate the identification of phenolics. Although MS detection was characterised by relatively poor sensitivity (full scan mode) and linear response, this form of detection proved to be an exceedingly powerful identification tool, allowing identification of 20 non-standard compounds in wine. These include procyanidins, epigallocatechin, prodelphinidins (only the second time these compounds are identified in wine), tartaric acid esters of p-coumaric acid and caffeic acid, 3 resveratrol derivatives, myricetin and flavonol-glucosides. It is concluded LC-UV-MS currently represents the state-of-the art in analysis methods for wine polyphenols, combining quantitative and identification capabilities.
The LC-diode array method was subsequently used to quantify 16 phenolic compounds in 55 South African red wines. Values are compared to those reported in the literature for these compounds in wines from other countries. These data were used together with chemometric methods for the characterisation of these wines according to variety. Discriminant analysis allowed a 100% correct recognition of the 5 cultivars studied, independent of the vintage or geographical origin, based on non-coloured phenolic content.

DOI:

Publication date: January 12, 2022

Issue: Terroir 2004

Type: Article

Authors

A. de Villiers (1), A. Crouch (1), Th. Heidemann (1), P. Majek (2), P. Sandra (1)

(1) University of Stellenbosch, Department of Chemistry, CENSSUS, Private Bag X1, Matieland 7602, South Africa
(2) Slovak University of Technology, Department of Analytical Chemistry, 812 37 Bratislava 1, Slovak Republic

Contact the author

Tags

IVES Conference Series | Terroir 2004

Citation

Related articles…

VineyardFACE: Investigation of a moderate (+20%) increase of ambient CO2 level on berry ripening dynamics and fruit composition

Climate change and rising atmospheric carbon dioxide concentration is a concern for agriculture, including viticulture. Studies on elevated carbon dioxide have already been on grapevines, mainly taking place in greenhouses using potted plants or using field grown vines under higher CO2 enrichment, i.e. >650 ppm. The VineyardFACE, located at Hochschule Geisenheim University, is an open field Free Air CO2 Enrichment (FACE) experimental set-up designed to study the effects of elevated carbon dioxide using field grown vines (Vitis vinifera L. cvs. Riesling and Cabernet Sauvignon). As the carbon dioxide fumigation started in 2014, the long term effects of elevated carbon dioxide treatment can be investigated on berry ripening parameters and fruit metabolic composition.
The present study aims to investigate the effect on fruit composition under a moderate increase (+20%; eCO2) of carbon dioxide concentration, as predicted for 2050 on both Riesling and Cabernet Sauvignon. Berry composition was determined for primary (sugars, organic acids, amino acids) and secondary metabolites (anthocyanins). Special focus was given on monitoring of berry diameter and ripening rates throughout three growing seasons. Compared to previous results of the early adaptative phase of the vines [1], our results show little effect of eCO2 treatment on primary metabolites composition in berries. However, total anthocyanins concentration in berry skin was lower for eCO2 treatment in 2020, although the ratio between anthocyanins derivatives did not differ.
[1] Wohlfahrt Y., Tittmann S., Schmidt D., Rauhut D., Honermeier B., Stoll M. (2020) The effect of elevated CO2 on berry development and bunch structure of Vitis vinifera L. cvs. Riesling and Cabernet Sauvignon. Applied Science Basel 10: 2486

The« Sigales’ method »

Le comportement de la vigne est étroitement lié aux propriétés hydriques des sols surtout dans leurs parties profondes.

Ozone treatment: a solution to improve sanitary and physiological quality of vine plant

The vineyard world is faced to a lot of fungal diseases. Grapevine Trunk Diseases (GTD) are some of the major. After exhibiting chronical foliar symptoms, grapevines can die by apoplexy within only few days. A range species of fungi was described to be associated with the apparition of early symptoms of GTD. It is well known that ozone dissolved into water is a powerful disinfectant with no remanence. The main goal of this study was to test the efficiency of this process on different fungal species associated with GTD in vitro and in planta conditions.

Digital PCR: a tool for the early detection of brettanomyces in wine

Brettanomyces bruxellensis is found in various ecological niches, but particularly in fermentative processes: beer, kombucha, cider and wine. In the oenological sector, this yeast is undesirable, as it can produce ethyl phenols, thus altering wine quality. These compounds are characterized by stable or horse-sweat aromas, unpleasant for consumers.

Monitoring vineyard canopy structure by aerial and ground-based RGB and multispectral imagery analysis

Unmanned Aerial Vehicles (UAVs) are increasingly used to monitor canopy structure and vineyard performance. Compared with traditional remote sensing platforms (e.g. aircraft and satellite), UAVs offer a higher operational flexibility and can acquire ultra-high resolution images in formats such as true color red, green and blue (RGB) and multispectral. Using photogrammetry, 3D vineyard models and normalized difference vegetation index (NDVI) maps can be created from UAV images and used to study the structure and health of grapevine canopies. However, there is a lack of comparison between UAV-based images and ground-based measurements, such as leaf area index (LAI) and canopy porosity.