IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 The interaction between wine polyphenolic classes and poly-L-proline is impacted by oxygen

The interaction between wine polyphenolic classes and poly-L-proline is impacted by oxygen

Abstract

Oxygen plays a key role in the evolution of wine chemistry, within the non-volatile matrix. Polyphenol composition and structure, as well as the process of tannin polymerisation are directly impacted by oxidation, and this can occur during both fermentation and ageing. Polyphenols play an important role in red wine and exhibit a wide diversity in their structure and properties. They are responsible for wine colour, texture and taste (astringency, bitterness) and exhibit some health properties. The principal class of non-flavonoid polyphenolic compounds are the phenolic acids and stilbenes. Among the flavonoids, anthocyanins and tannins are the major structural classes. The aim of this study was to characterise the detailed response of wine polyphenolic structure and composition to an oxygen treatment applied during fermentation. A specific focus was to determine the interaction of discrete polyphenolic classes with poly-L-proline (PLP). A control Shiraz wine was prepared under reductive conditions during fermentation, in triplicate. To the same grape source, an aeration treatment was initiated on day 3 following a 1.8 °Bé decrease for 48 h at 5 L/min, also in triplicate.  After a 12-month ageing period, wines were fractionated where: F1 = Phenolic acids, F2 = flavan-3-ol monomers, F3 = flavan-3-ol oligomers, F4 = anthocyanins, pyranoanthocyanins; and F5 = polymeric proanthocyanidins, pigmented proanthocyanins and other derived complexes. The composition of fractions F1 to F4 was verified by LC-MS, and F5 was characterised by a combination of analytical techniques specific to proanthocyanidins. The interaction between the polyphenol fractions and PLP was measured by isothermal titration calorimetry (ITC). A strong binding interaction was observed between F1, the phenolic acids, and PLP by ITC, and was not affected by the oxygen treatment. In fact, a strong hydrophobic interaction and hydrogen bonding was implicated in the interaction. It was found that for fractions F2 and F3, no binding events with PLP were observed by ITC, irrespective of the oxygen level applied. Stronger binding events with PLP were observed for the F4 and F5 polyphenolic fractions, but interestingly, only in those prepared from wines which had oxygen treatment. Moreover, hydrophobic interaction and hydrogen bonding was detected just for the oxygen treatment for F4 and F5. Contrary to expectation, no binding with PLP could be detected for F4 and F5 from the control wine. Further investigation of the properties of the fractions was conducted to account for the differences observed, including their composition, hydrophobicity and aggregation. This presentation will provide new insights into the potential role of discrete polyphenolic classes in driving in-mouth sensory properties, like astringency, which might be elicited following binding with proline-rich salivary proteins.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Jouin Alicia1, Falconer Robert J.2, Waterlot Aude3, Day Martin1, Schmidt Simon1 and Bindon Keren1

1The Australian Wine Research Institute, PO Box 197, Glen Osmond, South Australia, 5064, Australia 
2Department of Chemical Engineering and Advanced Materials, University of Adelaide, Adelaide, SA, 5005, Australia
3Department of Food Science and Human Nutrition, Courtesy Faculty, Horticulture, Iowa State University, 2567 Food Sciences Building, 536 Farm House Lane, Ames, IA 50011, USA

Contact the author

Keywords

Tannins, Anthocyanins, Oxygen, Isothermal Titration Calorimetry, Astringency

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Ripening of cv. Cabernet Sauvignon grapes: polysaccharides fractions evolution and phenolic extractability

Polysaccharides and more specifically pectins, make up a significant portion of the cell wall material of the plant cells including the grapes. During the fruit ripening the associated softening is related to the breakdown of the cell wall polysaccharides. During this process, it is expected that polysaccharides that are soluble in red wine will be formed influencing its texture. Anthocyanins are responsible for the wine color and tannins for the astringency, body and bitterness of the wine. In the skins, these compounds are located in the cell vacuoles and the barrier that conditions their extractability is the skin cell wall that may determine the mechanical resistance, the texture and the ease of processing berries. The aim of this work was study the evolution of the polysaccharides and the anthocyanin and tannin extractability during the ripening period in Cabernet Sauvignon grapes, trying to correlate these variables.

Wine shaking during transportation: influence on the analytical and sensory parameters of wine

According to OIV reports, annual world wine consumption fluctuated around 240-245 mln hL over the past decade. The general market globalization has led to the situation when almost half of the consumed wine is exported to other countries. Of this volume, more than 60 mln hL are bottled still and sparkling wines.

The relationship between wind exposure and viticultural performance of Vitis vinifera L. cv. Merlot in a coastal vineyard (South Africa)

The South Western Cape of South Africa is exposed to strong southerly and south easterly synoptic winds during the growth period of the grapevine. The development of sea breezes in the afternoon is also a phenomenon associated with the ripening period of grapes cultivated in this coastal area. Wind is one of the environmental variables having the greatest spatial variation but the implications of regular exposure to wind for the performance of the grapevine has not yet been determined for vineyards in the South Western Cape. This study was initiated to meet this need.
The study was conducted in a hedge-trellised vineyard of Vitis vinifera L. cv Merlot with north east – south west row direction. Thirty experimental sites, each consisting of 14 vines, were identified as being exposed to wind or sheltered based on hand-held anemometer readings during the 2001/2002 season. Four stationary anemometers were strategically positioned between the thirty sites. Stomatal conductance and leaf temperature were measured with a PP systems porometer. Vegetative and yield measurements were performed during the 2002/2003 season. The t-test of equal variance was used to determine significant differences in measured parameters between exposed and sheltered grapevines.
Stomatal conductance and leaf area were significantly reduced by exposure to wind. This was associated with a significant reduction in the leaf area of primary shoots, related to shorter shoots, but a significant augmentation of secondary shoot leaf number and area. The number of bunches per vine and yield were also reduced for exposed vines. The berry potassium content was significantly increased for exposed grapevines.
This demonstrates that exposure to wind can result in significant within-vineyard, and potentially between-vineyard, variability in grapevine physiology, vegetative growth, yield and berry composition, with implications for wine style and quality.

Incidences of the climate, the soil and the harvest date on Colombard aromatic potential in Gascony

This experiment tries to characterize the role of soil, climate and harvest date on the composition of grape-derivated thiols, 3-mercapto-hexanol (3MH) and 3-mercapto-hexile acetate (A3MH), in the white wines from Colombard varieties in Gascony (South-West of France). A network of 6 plots has been observed since 1999 on different pedologic units. The plots have common agronomical characteristics, plantation spacing (2,900 to 3,500 vines per ha), plantation aging (1985-1990), strength conferred by rootstock (SO4, RSB), soil management (grass covered 1 by 2) and training system (vertical shoot positionning pruned in single Guyot). Meteorological stations are located near the plots.

Sustainable wine industry: supercritical fluid extraction as key technology for biorefinery enhancement

Supercritical carbon dioxide (sc-CO2) extraction is an environmentally friendly technology employed for bioactive compounds recovery from various natural sources and biomasses. The advantages of sc-co2 extraction include its selectivity, relatively mild operating conditions, which minimize the degradation of sensitive compounds, and the absence of potentially harmful organic solvents.