Vitamins in grape must: let’s lift a corner of the veil

This work, included in the VAVEGEX project, aims to evaluate the oenological, phenolic, chromatic and sensory characteristics of the grapes, must and wines produced from cv. Tortojona, minority variety grown in Extremadura region (Southwest, Spain).

Aims: Microscopic bud dissection is a common tool used to assess grapevine bud fertility and therefore to predict the yield of the following season

During oak wood contact, wine undergoes important modifications that modulate its organoleptic quality and complexity, including its aroma, structure, astringency, bitterness and color. Vescalagin and castalagin are the two main C-glucosidic ellagitannins found in oak wood used for wine aging wood but lyxose/xylose derivatives (grandinin and roburin e) and dimeric forms (roburins a,b, c and d) are also present. The presence of several hydroxyl groups in the ortho-positions at the periphery of the structure of the ellagitannin isomers allows these molecules to undergo oxidation or condensation reactions with other compounds.

Heat and water stress can accelerate berry sugar accumulation and lead to excessive sugar-to-acid ratios at harvest, producing bland, overly-alcoholic wines. Selecting grapevines for slower sugar accumulation could help maintain wine quality under future, hotter conditions, but these efforts have been stymied by our limited understanding of the traits determining sugar accumulation rates. Here, we measured traits characterizing the structure and anatomy of the sugar transport system – the phloem – in 16 winegrape cultivars and tested for relationships with sugar accumulation rates and cultivar climate classifications.

All techniques usually used to assay proteins was not reliable in vegetable extract due to interferences with the components included in extracts like polyphenols, tanins, pectines, aromatics compounds. Absorbance at 280nm, Kjeldhal assay, Biuret and Lowry methods, Acid Bicinchonique technique and Bradford assay give the results depending on the composition of extract, on the presence or not of detergent and on the raw material (Marchal, 1995). Another difficulty in these extracts for the quantification of proteins comes from the large amount of water included in vegetable and the low concentration of proteins. Thus in red wines, proteins are usually not taken into account due to their low concentration (typically below 10 mgL-1) and to the presence of anthocyanis and polyphenols.