IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Antifungal and Laccase-Suppressing Activity of Phenolic Compounds and Their Oxidation Products on Grey Mold-Fungus Botrytis cinerea

Antifungal and Laccase-Suppressing Activity of Phenolic Compounds and Their Oxidation Products on Grey Mold-Fungus Botrytis cinerea

Abstract

Botrytis cinerea causes grey mold that results in severe problems for wine makers worldwide. Infected grapes lead to quality deterioration including formation of off-flavors or browning. The latter is caused by the enzyme laccase which is capable of oxidizing a wide range of phenolic compounds. Since the use of conventional pesticides is associated with many concerns of consumers and authorities regarding environmental and health related issues and may result in fungicide resistance, the development of green alternatives is gaining more attention. Phenolic compounds like ferulic acid or stilbens which serve as phytoalexins have shown promising results in terms of growth-inhibitory effects against B. cinerea. The present study focuses on further investigations of the antifungal and laccase-suppressing effects of these phenols and especially their laccase-derived oxidation products using the syringaldazine-assay for determination of the laccase activity. The results revealed that they can be considered as a sustainable alternative to synthetic fungicides for Botrytis cinerea management.

DOI:

Publication date: June 27, 2022

Issue: IVAS 2022

Type: Poster

Authors

Umberath Kim Marie1, Schieber Andreas1 and Weber Fabian1

1University of Bonn, Institute of Nutritional and Food Sciences, Molecular Food Technology

Contact the author

Keywords

Botrytis cinerea, laccase, phenolic compounds, oxidation products, antifungal activity

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Defoliation timing impacts berry secondary metabolites and sunburn damage

Sunburn is a physiological disorder that leads to yield and quality losses in a range of fruits such as grapes and apples. It affects the visual appearance and the composition of the fruit, leading to irreversible changes and ultimately, cell death in extreme situations.

Influence of a spontaneous cover crop on the vineyard and soil erosion under Mediterranean climate

Sixty five % of the agricultural area of the Basque Country located in the DO Ca Rioja corresponds to vineyards. More than 40% of it has an average slope greater than 10%, which makes it sensitive to erosive processes. Furthermore, it is foreseeable that extreme weather events (storms, hail, extreme heat and cold, etc.) will be favored due to climate change. Cover cropping can mitigate this risk, and therefore the objective of this work is to evaluate the impact that a vegetable cover has on the agronomic behavior of the vineyard, the quality of the grape and soil erosion. For this, a trial has been carried out with a Graciano variety vineyard with a slope between 10% -20% during the years 2020 and 2021. Conventional tillage management in the area has been compared (4-6 passes per year of tillage machinery) versus spontaneous vegetation cover management in the vineyard. This implies not tilling and allowing the grass of the land to colonize the range between the lines of vines, controlling their height through 1-3 mowing passes per year, always trying to affect the surface of the land as little as possible. The vegetative growth, yield and quality of the grape and wine was measured. Furthermore, erosion has been measured using Gerlasch boxes. The yield was lower in the second year of the trial in the cover crop treatment, but erosion was significantly reduced.

Research summary on the use of Terroir as a wine purchasing cue

Due to the current challenging nature of the global wine market, and recent growth in number and strength of competitors from non-traditional wine producing countries, European wine producers are focussing on the potential to develop a competitive advantage through the concept of terroir.

Data mining approaches for time series data analysis in viticulture. Potential of the bliss (Bayesian functional linear regression with sparse step functions) method to identify temperature effects on yield potential

Context and purpose of the study – Vine development, and hence management, depends on dynamic factors (climate, soil moisture, cultural practices etc.) whose impact can vary depending upon their temporal modalities.

Oenological features of Sangiovese wine from vinification of whole grape berries

The present study was performed in a traditional winery located in the viticultural area of Brunello di Montalcino, Siena, Italy, in the vintage 2015. Actually, in this winery Sangiovese grape musts are fermented in large oak barrels by a single strain of Saccharomyces cerevisiae previously isolated in the same winery. Pumping over operations are carried out once or twice a day until the end of alcoholic fermentations. The aim of this work was to investigate on the oenological properties of Sangiovese wine produced with the traditional winemaking process adopted by the winery under study obtained from the fermentation of whole berries compared to that from crushed grape must. In particular, two lots of 65q of Sangiovese grapes from the same 3ha vineyard were vinified in 150hL oak barrels.