GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Protection of grapevines from red blotch by understanding mechanistic basis of its infection

Protection of grapevines from red blotch by understanding mechanistic basis of its infection

Abstract

Context and purpose of the study – Currently, grapevine is host to a large number of pathogenic agents, including 65 viruses, five viroids and eight phytoplasmas. Needless to say, these pathogens, especially viruses responsible for several ‘infectious degeneration’ or ‘decline’ cause great distress to wine makers and grape growers, let alone the large economic losses incurred by the wine industry. A recent addition to this wide repertoire of grapevine viruses is a new viral disease known as Red Blotch in viticulture parlance. Its causal organism, Grapevine red blotch associated virus (GRBaV), discovered in 2008 is a newly identified virus of grapevines and a putative member of a new genus within the family Geminiviridae. Typical symptoms in red cultivars include red blotches in leaves of afflicted vines with pinkish red-colored veins without any rolling of the margins observed at the onset of ripening. The objective of this study was to determine as to how the virus enters grapevine and once it enters the vine, how it is distributed throughout the vine.

Material and methods – During the growing season, vine samples were collected from vineyards with a history of Red Blotch (both red and white cultivars) located in Napa/Sonoma counties and the state of Washington. Starting at flowering, shoots (leaf and stem tissues) were sampled for microscopy analysis. These samples were used to determine the structure and functionality of the vascular strands (xylem and phloem) using callose specific dye, aniline blue and various techniques of microscopy.

Results – The afflicted fruits were high in acid but low in sugars causing delay in harvest. Canematuration was poor as evident from uneven browning characterized by areas of green and brown color. Typically, green stems fail to turn brown when the periderm is not formed indicating that the GRBaV interferes with cane maturation process. Callose (a carbohydrate substance) accumulated in the phloem cells of afflicted vines. Such deposition indicated that the GRBaV is primarily confined to phloem sieve tubes. Since callose deposits limit pathogen dispersal, this phenomenon could be used as a diagnostic indication of Red Blotch. Similarly, in the context of vascular blockage, the xylem vessels of afflicted canes showed tylosis, which is ballooning of neighboring xylem parenchyma cells into the lumen of the vessels. These observations indicated that even though the GRBaV enters grapevine via the phloem, both xylem and phloem responded to the virus invasion. This is an interesting observation and needs further investigation as tylosis typically occurs only when the xylem is injured either by pruning or occluded with bacteria. Vessels of healthy canes remained free of tylose. These results added a new dimension to viral diseases, especially Red Blotch and might provide a framework for developing management strategies to minimize the incidence of Red Blotch in grape growing regions worldwide.

DOI:

Publication date: September 28, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Bhaskar BONDADA1*, Paul SKINNER2, Marc FUCHS3, and Andrew WALKER4

1 Washington State University, Richland, WA 99354, USA
2 Vineyard Investigations, St. Helena, CA 94574, USA
3 Cornell University, Geneva, NY 14456, USA
4 University of California, Davis, CA 95616, USA

Contact the author

Keywords

callose, grapevine, phloem, red blotch, tylosis, xylem

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Photoselective shade films affect grapevine berry secondary metabolism and wine composition

Grapevine physiology and production are challenged by forecasted increases in temperature and water deficits. Within this scenario, photoselective overhead shade films are promising tools in warm viticulture areas to overcome climate change related factors. The aim of this study was to evaluate the vulnerability of ‘Cabernet Sauvignon’ grape berry to solar radiation overexposure and optimize shade film use for berry integrity. A randomized complete block design field study was conducted across two years (2020-2021) in Oakville, Napa Valley, CA, with four shade films (D1, D3, D4, D5) differing in the percent of radiation spectra transmitted and compared to an uncovered control (C0). Integrals for gas exchange parameters and mid-day stem water potential were unaffected by the shade films in 2020 and 2021. By harvest, berries from uncovered and shaded vines did not differ in their size or primary metabolism in either year. Despite precipitation exclusion during the dormant season in the shaded treatments, yield did not differ between them and the control in either season. In 2020, total skin anthocyanins (mg/g fresh mass) in the shaded treatments was greater than C0 during berry ripening and at harvest. Conversely, flavonol concentrations in 2020 were reduced in shaded vines compared to C0. The 2020 growing season highlighted the impact of heat degradation on flavonoids. Flavonoid concentrations in 2021 increased until harvest while flavonoid degradation was apparent from veraison to harvest in 2020 across shaded and control vines. Wine analyses highlighted the importance of light spectra to modify wine composition. Wine color intensity, tonality and anthocyanin values were enhanced in D4 whereas antioxidant properties were enhanced in C0 and D5 wines. Altogether, our results highlighted the need of new approaches in warm viticulture areas given the impact that composition of light has on berry and wine quality.

Combining effect of leaf removal and natural shading on grape ripening under two irrigation strategies in Manto negro (Vitis vinifera L.)

The increasingly frequent heat waves during grape ripening pose challenges for high quality wine grape production. Defoliation is a common practice that can improve the control of diseases in bunches, but also it increases the exposure to sunlight. Grapes exposed to solar radiation reach temperatures over the optimum for berry development and maturation. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 using Manto negro wine grapes to study the effect of applied irrigation and different light exposure levels on grape quality. Two irrigation treatments were imposed based on the frequency and amount of water doses in a four-block experimental vineyard at Bodega Ribas (Mallorca). Three light exposure treatments were randomly applied in each irrigation plot. The light treatments included exposed clusters from pea size, non-exposed clusters, and shaded clusters after softening. Leaf area index and canopy porosity was estimated every 2 weeks. Midday leaf water potential was measured weekly. Additionally, apparent electrical conductivity was measured between rows to estimate the soil water content variability. Light and temperature sensors were installed at the bunch level to quantify the differences in bunch temperature and light intensity among treatments. The effect of irrigation and cluster light exposure on berry weight, TSS, TA, malic acid, tartaric acid, K+, and pH were analysed at 5 moments along grape ripening. During different heat waves, the natural shading technique decreased the maximum bunch temperature around 10 °C respect to the exposed bunches in both irrigation strategies. The combination of defoliation and shading techniques after softening decreased TSS at harvest and affected most of the quality parameters during the last stages of ripening, showing an interesting technique to delay ripening in warm viticulture areas.

Extreme canopy management for vineyard adaptation to climate change: is it a good idea?

Climate change constitutes an enormous challenge for humankind and for all human activities, viticulture not being an exception. Long-term strategic changes are probably needed the most, but growers also need to deal with short-term changes: summers that are getting progressively warmer, earlier harvest dates and higher pH in musts and wines. In the last 10-15 years, a relevant corpus of research is being developed worldwide in order to evaluate to which extent extreme canopy management operations, aimed at reducing leaf area and, thus, limiting the source to sink ratio, could be useful to delay ripening. Although extreme canopy management can result in relevant delays in harvest dates, longer term studies, as well as detailed analysis of their implications on carbohydrate reserves, bud fertility and future yield are desirable before these practices can be recommended.

Towards a regional mapping of vine water status based on crowdsourcing observations

Monitoring vine water status is a major challenge for vineyard management because it influences both yield and harvest quality. It is also a challenge at the territorial scale for identifying periods of high water restriction or zones regularly impacted by water stress. This information is of major importance for defining collective strategies, anticipating harvest logistic or applying for irrigation authorisation. At this spatial scale, existing tools and methods for monitoring vine water status are few and often require strong assumptions (e.g. water balance model). This paper proposes to consider a collaborative collection of observations by winegrowers and wine industry stakeholders (crowdsourcing) as an interesting alternative. Indeed, it allows the collection of a large number of field observations while pooling the collection effort. However, the feasibility of such a project and its interest in monitoring vine water status at regional scale has never been tested.

The objective of this article is to explore the possibility of making a regional map of vine water status based on crowdsourcing observations. It is based on the study of the free mobile application ApeX-Vigne, which allows the collection of observations about vine shoot growth. This information is easy to collect and can be considered, under certain conditions, as a proxy for vine water status. This article presents the first results obtained from the nearly 18,000 observations collected by winegrowers and wine industry stakeholders during 2019, 2020 and 2021 seasons. It presents the vine shoot growth maps obtained at regional scale and their evolution over the three vintages studied. It also proposes an analysis of the factors that favoured the number of observations collected and those that favoured their quality. These results open up new perspectives for monitoring vine water status at a regional scale but above they provide references for other crowdsourcing projects in viticulture.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.