GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Effect of irrigation regime on carbon isotope ratio (δ13c) in different grapevines

Effect of irrigation regime on carbon isotope ratio (δ13c) in different grapevines

Abstract

Context and purpose of the study – In Castilla-La Mancha as other winegrowing regions, vineyards suffer the effects of the global climate warming. Severe spring and summer droughts are increasingly frequent, which concur with the phenological stages most susceptible to water and temperature stress. Under these conditions, irrigation use is required in order to ensure the vineyard growing sustainability. However water resources are increasingly limited, for this reason is required to choose cultivars displaying high water use efficiency. Carbon isotope ratio (δ13C) of grape must sugar has been investigated as an integrating marker related to water use efficiency and water status in grapevines. The present study was aimed to explore water use efficiency in several cultivars subjected to different irrigation regimes, in order to know those that were more efficient and subsequently develop specific watering protocols for each of them, according to sustainable production and quality goals.

Material and methods – This study was carried out in 2017 and 2018. Variety response of δ13C to different irrigation regime was assessed in a multivarietal vineyard. Grown on trellises, at a distance of 2.8m x 1.2m (row by vine spacing), the plants are trained to a single guyot system, with 110 Richter as rootstock. Orientation is 30oNE/210oSW and the vineyard is irrigated by a drip system with two drippers per vine-stock. Four treatments were considered: survival, 0.20 ET0, 0.25 ET0 and 0.30 ET0. Determination of the carbon isotope ratios of grape must was carried out by on-line analysis using a ThermoQuest Flash 1112 elemental analyser equipped with an autosampler and coupled to a Delta-Plus IRMS (ThermoQuest) through a ConFlo III interface (ThermoQuest). In addition to δ13C in must sugar, yield components and must quality parameters were determined for each treatment and variety.

Results – Irrigation promoted a decrease of carbon isotope ratio in must sugar. The relationship between δ13C and water volumes used in irrigation treatments was negative and moderately significant. Considering the data of two vintages together and treatment as a variable, the effect of irrigation regime in carbon isotope ratio was observed in all cases with significant differences ranging from -22.58 for T0 to -24.48 for T3, whereas in WUE only T0 (30.15 g/L) stood out from the rest (12.86 g/L, 10.84 g/L and 10.32 g/L for T1, T2 and T3 respectively). On the contrary, when grapevine variety was a variable, there were only significant differences in δ13C when considering vintages independently. It was in 2017, with values ranging from -23.52 for Airén to -24.69 for Moscatel de Grano Menudo. Regarding WUE, in neither of two vintages separately there were significant differences. Between δ13C and agronomic parameters there were some correlations with different significance levels. This study contributes to improving knowledge about what of the cultivars grown in the areaare more efficient from the water use point of view, and the irrigation regimes that would have to be established to achieve sustainable production, both quantitatively and qualitatively, with the minimum water volume.

DOI:

Publication date: September 18, 2023

Issue: GiESCO 2019

Type: Poster

Authors

Juan Luis CHACÓN*, Jesús MARTÍNEZ, Adela MENA

Instituto de la Vid y el Vino de Castilla-La Mancha (IVICAM), Tomelloso, Spain

Contact the author

Keywords

carbon isotope ratio, grapevine, irrigation, Vitis vinifera L., water use efficiency

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Protected Designation of Origin (D.P.O.) Valdepeñas: classification and map of soils

The objective of the work described here is the elaboration of a map of the different types of vineyard soils that to guide the famers in the choice of the most productive vine rootstocks and varieties. 90 vineyard soils profiles were analysed in the entire territory of the Origen Denominations of Valdepeñas. The sampling was carried out in 2018 (June to October) by making a sampling grid, followed by photointerpretation and control in the field. The studied soils can be grouped into 9 different soil types (according to FAO 2006 classification): Leptosols, Regosols, Fluvisols, Gleysols, Cambisols, Calcisols, Luvisols and Anthrosols. A map showing the soil distribution with different type of soils has been made with the ArcGIS program. Regarding to the choice of rootstock, Calcisoles are soils with a high active limestone content, so the rootstocks used in these soils must be resistant to this parameter; Luvisols are deep soils with high clay content, so they will support vigorous rootstocks. Because the cartographic units are composed of two or more subgroups, with are associated in variable proportions, 9 different soil associations have been established; Unit 1: Leptosols, Cambisols and Luvisols (80%, 15% and 5% respectively); Unit 2: Cambisols with Regosols and Luvisols (40%, 30% and 30% respectively); Unit 3: Cambisols and Gleysols with Regosols (40%, 40% and 20% respectively); Unit 4: Regosols with Cambisols, Leptosols and Calcisols (40%, 30%, 15% and 15% respectively); Unit 5: Cambisols, Leptosols, Calcisols and Regosols (25% each of them); Unit 6: Luvisols with Cambisol and Calcisols (80%, 10% and 10% respectively); Unit 7: Luvisols and Calcisols with Cambisols (40%, 40% and 20% respectively); Unit 8: Calcisols with, Cambisols and Luvisols (80%, 10% and 10% respectively); Unit 9: Anthrosols. These study allow to elaborate the first map of vineyard soils of this Protected Designation of Origin in Castilla-La Mancha.

How distinctive are single vineyard Gewürztraminer musts and wines from Alto Adige (Italy) based on untargeted analysis, sensory profiling, and chemometric elaboration?

Vitis vinifera L. ‘Gewürztraminer’ is a historical grape variety of Alto Adige (Südtirol), Italy, which is widely grown in the area of Tramin an der Weinstraße, but is also grown globally. It produces highly aromatic wines that are strongly influenced by the terroir of the vineyard sites where they are grown. This study looked at musts and young wines from ‘Gewürztraminer’ grapes harvested in seven distinct vineyards near Tramin and then processed at Cantina di Termeno, minimizing winemaking protocol variability. Samples were profiled using bidimensional gas chromatography–time-of-flight mass spectrometry, liquid chromatography coupled to electrochemical detection, and near-IR spectrometry. The data were subjected to Principle Component Analysis and Hierarchical Clustering Analysis. Sensory discriminant testing was undertaken using the sorting method with a semi-trained panel, and the data were processed using Multidimensional Scaling. Seven must/wine pairs could be distinguished based on their untargeted volatilome profiles and on sensory evaluation. As expected, there were greater differences in the volatile compounds between the wines than between the musts. The wines from vineyards 4 and 5 were nonetheless quite homogenous in terms of chemical and sensory analyses, as were the wines from vineyards 1 and 3. For the phenolic profile, differences were noted between the musts and wines of vineyards 2, 3, and 4, but the musts from vineyards 5 and 7 were similar. Sensory analysis showed the wines from vineyards 6 and 7 to be distinct from the rest. These results reinforce that the composition of ‘Gewürztraminer’ musts and wines is strongly determined by vineyard site, even in a small geographic area with high variability of the terroir (soil and microclimate), and that these differences are apparent in the flavours and aromas of the finished wines. Further confirmation would require a larger sample of wines, preferably from several vintages.

Mobile device to induce heat-stress on grapevine berries

Studying heat stress response of grapevine berries in the field often relies on weather conditions during the growing season. We constructed a mobile heating device, able to induce controlled heat stress on grapes in vineyards. The heater consisted of six 150 W infrared lamps mounted in a profile frame. Heating power of the lamps could be controlled individually by a control unit consisting of a single board computer and six temperature sensors to reach a pre-set temperature. The heat energy applied to individual berries within a cluster decreases by the squared distance to the heat source, enabling the establishment of temperature profiles within individual clusters. These profiles can be measured by infrared thermography once a steady state has been reached. Radiant flux density received by a berry depending on the distance was calculated based on a view factor and measured lamp surface temperature and resulted to 665 Wm-2 at 7cm. Infrared thermography of the fruit surface was in good agreement with measurements conducted with a thermocouple inserted at epidermis level. In combination with infrared thermography, the presented device offers possibilities for a wide range of applications like phenotyping for heat tolerance in the field to proceed in the understanding of the complex response of plants to heat stress. Sunburn necrosis symptoms were artificially induced with the aid of the device for cv. Bacchus and cv. Sylvaner in the 2020 and 2021 growing season. Threshold temperatures for sunburn induction (LT5030min) were derived from temperature data of single berries and visual sunburn assessment, applying logistic regression. A comparison of threshold temperatures for the occurrence of sunburn necrosis confirmed the higher susceptibility of cv. Bacchus. The lower susceptibility of cv. Sylvaner did not seem to be related to its phenolic composition, rendering a thermoprotective role of berry phenolic compounds unlikely.

Pruned vine biomass exclusion from a clay loam vineyard soil – examining the impact on physical/chemical properties

The wine industry worldwide faces increasing challenges to achieve sustainable levels of carbon emission mitigation. This project seeks to establish the feasibility of harvesting winter pruned vineyard biomass (PVB) for potential use in carbon footprint reduction, through its use as a renewable biofuel for energy production. In order to make this recommendation, technical issues such as the potential environmental impact, chemical composition and fuel suitability, and logistical challenges of harvesting biomass needs to be understood to compare with the results from similar studies. Of particular interest is the role PVB plays as a carbon source in vineyard soils and what effect annual removal might have on soil carbon sequestration. A preliminary trial was established in the Waite Campus vineyard (University of Adelaide) to test current management strategies. Vines are grown in a Eutrophic, Red Dermosol clay loam soil with well managed midrow swards. A comparison was undertaken of mid-row treatments in two 0.25 Ha blocks (Shiraz and Semillon), including annual cultivation for seed bed preparation, the deliberate exclusion of PVB (25 years) and incorporation of PVB (13 years) at an average of 3.4 and 5.5 Mg/Ha-1 for Shiraz and Semillon respectively. In both 0-10cm and 10-30cm soil core sample depths, combined soil carbon % measures in the desired range of 1.80 to 3.50, were not significantly different between treatments or cultivars and yielded an estimated 42 Mg/ha-1 of sequestered soil carbon. Other key physical and chemical measures were likewise not significantly different between treatments. Preliminary results suggest that in a temperate zone vineyard, managed such as the one used in this study, there is no long term negative impact on soil carbon sequestration through removing PVB. This implies that growers could confidently harvest PVB for use in several end fates including as a bio fuel.

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…