terclim by ICS banner
IVES 9 IVES Conference Series 9 Microbial ecosystems in wineries – molecular interactions between species and modelling of population dynamics

Microbial ecosystems in wineries – molecular interactions between species and modelling of population dynamics

Abstract

Microbial ecosystems are primary drivers of viticultural, oenological and other cellar-related processes such as wastewater treatment. Metagenomic datasets have broadly mapped the vast microbial species diversity of many of the relevant ecological niches within the broader wine environment, from vineyard soils to plants and grapes to fermentation. The data highlight that species identities and diversity significantly impact agronomic performance of vineyards as well as wine quality, but the complexity of these systems and of microbial growth dynamics has defeated attempts to offer actionable tools to guide or predict specific outcomes of ecosystem-based interventions. The application of such tools in future will depend on our understanding of the physiological and molecular drivers that govern microbial ecosystems. Here we describe several integrated approaches to characterize the molecular interactions between species within the fermentation and the waste-water ecosystem and to model the development of these ecosystems. Binary (two species) and consortia-based approaches indicate ecosystem-specific developmental patterns in these systems. On a molecular level, data strongly support that cell-wall related properties of yeast species impact the development of fermentation ecosystems during wine making and highlight the importance of physical contacts between species in these ecological processes. To model the wine yeast fermentation ecosystem, high-throughput flow cytometry-based approaches were developed, and specific models based on a machine-learning approach were developed. In winery wastewater, laboratory-based evolution of two species exposed to biotic selection pressure in a synthetic environment, Saccharomyces cerevisiae and the microalga Chlorella sorokiniana, identified two specific genes involved in carbon and nitrogen catabolite repression that facilitate mutualistic behaviors between yeast and microalgae when inactive. Taken together the data suggest novel strategies for microbial ecosystem-based decision making in wine making and improved integration of natural microbial biodiversity in the process.

DOI:

Publication date: February 11, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Florian F. Bauer, Cleo Conacher, Jennifer Oosthuizen, Georgia Strydom, Evodia Setati, Rene Naidoo-Blassoples

South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University, Stellenbosch, South Africa

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

FLAVONOID POTENTIAL OF MINORITY RED GRAPE VARIETIES

The alteration in the rainfall pattern and the increase in the temperatures associated to global climate change are already affecting wine production in many viticultural regions all around the world (1). In fact, grapes are nowadays ripening earlier from a technological point of view than in the past, but they are not necessarily mature from a phenolic point of view. Consequently, the wines made from these grapes can be unbalanced or show high alcohol content. Dramatic shifts in viticultural areas are currently being projected for the future (2).

INVESTIGATING TERROIR TYPICITY: A COMPREHENSIVE STUDY BASED ON THE AROMATIC AND SENSORIAL PROFILES OF RED WINES FROM CORBIÈRES APPELLATION

Volatile compounds play a significant role on the organoleptic properties defining wines quality. This particular role was exploited in several studies with the aim to differentiate wines from a more or less extensive production area, according to their sensory profile [1], as well as their chemical composition [2,3] (Di Paola-Naranjo et al., 2011; Kustos et al., 2020). Indeed, since aroma compounds development in grapes depends primarily on the environmental conditions of the vines and grapes (soil and climate), it is conceivable that these parameters craft the aromatic signature of the wine produced, in relation to its origin (Van Leeuwen et al., 2020). In this work, a general study on the aromatic and sensorial profile of wines produced in five sub-regions of the Corbières denomination, a renowned red grape varieties viticultural region in South France, was reported.

FUNCTIONALIZED MESOPOROUS SILICA IS A VIABLE ALTERNATIVE TO BENTONITE FOR WINE PROTEIN STABILIZATION

The presence of grape-derived heat unstable proteins can lead to haze formation in white wines [1], an instability prevented by removing these proteins by adding bentonite, a hydrated aluminum silicate that interacts electrostatically with wine proteins leading to their flocculation. Despite effective, using bentonite has several drawbacks as the costs associated with its use, the potential negative effects on wine quality, and its environmental impact, so that alternative solutions are needed.

MAPPING OF GAS-PHASE CO₂ IN THE HEADSPACE OF CHAMPAGNE GLASSES BY USING AN INFRARED LASER SENSOR UNDER STATIC TASTING CONDITIONS

From the chemical angle, Champagne wines are complex hydro-alcoholic mixtures supersaturated with dissolved carbon dioxide (CO₂). During the pouring process and throughout the several minutes of tasting, the headspace of a champagne glass is progressively invaded by many chemical species, including gas-phase CO₂ in large majority. CO₂ bubbles nucleated in the glass and collapsing at the champagne surface act indeed as a continuous paternoster lift for aromas throughout champagne or sparkling wine tasting [1]. Nevertheless, inhaling a gas space with a concentration of gaseous CO₂ close to 30% and higher triggers a very unpleasant tingling sensation, the so-called “carbonic bite”, which might completely perturb the perception of the wine’s bouquet.

OENOLOGICAL STRATEGIES FOR THE REMOVAL OF PINKING IN WHITE WINE

The pinking of in white wine is the turning of color from yellow to salmon hue. White wines obtained from certain grape varieties (e.g. Chardonnay, Sauvignon blanc, Riesling, Trebbiano di Lugana) showed to be susceptible to pinking [1] that has been evaluated by an assay providing the addition of hydrogen peroxide. Even if its appearance does not seem to affect the sensory properties [2], strategies are necessary for its removal. Nowadays, the treatment with polyvinylpolipirroline (PVPP) was reported to significantly decrease the pink color [3].