GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2019 9 The state-of-the-art of grapevine biotechnology and new breeding technologies (NBTS)

The state-of-the-art of grapevine biotechnology and new breeding technologies (NBTS)

Abstract

Context of the review – The manipulation of the genetic basis controlling grapevine adaptation and phenotypic plasticity can be performed either by classical genetics or biotechnologies. In the last 15 years, considerable knowledge has accumulated about the grapevine genome as well as the mechanisms involved in the interaction of the vine with the environment, pests and diseases. Despite the difficulties associated with genetic mapping in this species (allele diversity, chimerism, long generation intervals…), several major QTLs controlling important vegetative or reproductive traits have been identified. Considering the huge genotypic and phenotypic diversities existing in Vitis, breeding offers a substantial range of options to improve the performances of cultivars. However, even if marker-assisted selection was largely developed to shorten breeding programs, the selection of improved cultivars, whether for agronomic traits or disease tolerances, is still long and uncertain. Moreover, breeding by crossing does not preserve cultivar genetic background, when the wine industry and market being still based on varietal wines.

Significance of the review – In grapevine, pioneering biotechnologies were set up in the 1960’s to propagate and/or clean the material from micro-organisms. In the 1990’s, the basis of genetic engineering was primary established through biolistic or Agrobacterium with several derived technologies refined in the last 10 years. The latest advance is represented by a group of technologies based on genome editing which allows a much more precise modification of the genome. These technologies, so-called NBT (new breeding technologies), which theoretically do not deconstruct the phenotype of existing cultivars, could be potentially better accepted by the wine industry and consumers than previous GMO approaches. This paper review the current state-of-the-art of the biotechnologies available for grapevine genome manipulation and future prospects for genetic improvement.

DOI:

Publication date: June 19, 2020

Issue: GiESCO 2019

Type: Article

Authors

Lorenza DALLA COSTA (1), Mickael MALNOY (1), David LECOURIEUX (2), Laurent DELUC (3), Fatma OUAKED- LECOURIEUX (2), Mark R. THOMAS (4) and Laurent TORREGROSA (5)

(1) Dept. of Biology and Genomic of Fruit Plants, Foundation E. Mach, 38010 San Michele all’Adige, Italy
(2) ISVV-EGFV, CNRS, INRA, Uni Bordeaux, 33883 Villenave d’Ornon, France
(3) Dept. of Horticulture, Oregon State University, OR 97331, Corvallis, USA
(4) CSIRO Agriculture and Food, Hartley Grove, Urrbrae SA 5064, Australia
(5) AGAP, Montpellier University, CIRAD, INRA, Montpellier SupAgro, Montpellier, France

Contact the author

Keywords

Grapevine, biotechnologies, gene transfer, genome editing, genetic improvement

Tags

GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Simulating single band multispectral imaging from hyperspectral imaging: A study into the application of single band visible to near-infrared multispectral imaging for determining table grape quality

To be accepted by the market and consumers table grapes need to meet certain requirements in terms of physical and chemical quality parameters.

Effect of one-year cover crop and arbuscular mycorrhiza inocululation in the microbial soil community of a vineyard

The microbial composition of the soil is an important factor to consider in viticulture, since its influence on the “terroir” and on the organoleptic properties of the wine have been demonstrated. Different agronomic techniques have the potential to modify the composition and functionality of the soil microbial community. Maintaining green covers is known to increase soil microbial diversity. The direct application of inoculum of beneficial microorganisms to the soil has also been used to increase their abundance. However, the environmental conditions of each site seem to have a determining weight in the result of these practices. In this study, we compared the effect on the microbial community of a cover crop with legumes in autumn and the inoculation of grapevines with commercial inoculum bases on Rhizophagus irregularis and Funeliformis mosseae in the previous spring. The study has been carried out in a vineyard in Binissalem, Mallorca, Spain. After applying the treatments, we will analyze the soil microbial communities using the data obtained from Illumina amplification of soil DNA from the 16S and ITS regions to analyze bacteria and fungi community, respectively. In addition, we will record the physicochemical characteristics of the soil at each sampling point. The result showed that agronomic management, in the short term, has less influence than soil characteristics on the composition of the soil microbiome. With these results, we can conclude that in a vineyard, agricultural techniques should focus on improving the characteristics of the soil to improve the biodiversity of the soil microbiota.

Mannoproteins from oenological by-products as tartaric stabilization and color agents in white and red wines

Climate change is drastically modifying grape composition and wine quality. As consequence, must and wines are becoming unbalanced, with high sugar concentration, increased alcohol content, lower acidity, excessive astringency, color instability and also a rise in the incidence of tartaric instability is being showed.

Influence of cover crops in a Tempranillo vineyard grown under the edaphoclimatic conditions of the Appellation of Origin Rueda

The way to manage the vineyard soils has certainly changed in Spain during the last years. Traditionally, the vineyards were tilled, but this growing technique has been replaced in some vineyards by the bare soil with herbicide

UNRAVELLING THE ROLE OF LACTIC ACID BACTERIA ON SPARKLING WINE ELABORATION THROUGH METABOLOMICS APPROACH

Xinomavro is a red grape variety from Northern Greece (Protected Designation of Origin), known for the nice acidities, perfectly appropriate for sparkling wine production (Rosé and Blanc de Noir). The elabo- ration of sparkling wine requires technical as well as scientific skills. Although the impact of the yeast strains and their metabolites on the final product quality is well documented, the action of bacteria still remains unknown.
The present work focuses (i) on the population diversity of lactic acid bacteria isolated from sparkling wines and (ii) on the technological effect of the species during sparkling wine elaboration.