Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

Abstract

Foam stability of sparkling wines is significantly favored by the presence of surface active agents such as proteins and polysaccharides [1]. For that reason, the renowned sparkling wines are aged after the second fermentation in contact with the lees for several months (even years). Thereby wines are enriched in these macromolecules due to yeast autolysis. Since this practice is slow and costly, winemakers are seeking for alternative procedures to increase their concentration in base wines. In that sense, the supplementation with inactive yeast during alcoholic fermentation has been proposed [2]. The aim of this study was to determine whether this new strategy is really useful for enriching base wines in macromolecules and for improving foam properties of the base wines. MATERIAL AND METHODS: Macabeo grapes were harvested at the appropriate maturity and pressed. The grape juice was immediately sulfited and pectinolytic enzymes were added to facilitate settling. After 24 h, clean grape juices (around 70 L each) were racked into nine 100-L stainless steel tanks and were fermented under controlled temperature (16-18 ºC) with selected yeasts. Three tanks were considered as controls whereas other 6 were supplemented with 40 g/hL of 2 inactive yeasts (3 with Optiwhite and 3 with Optimumwhite; Lallemand Inc., Montreal, Canada). Once the alcoholic fermentation was finished, wines were sulfited, racked and cold stabilized. Proteins were analyzed by HRSEC-DAD [3], polysaccharides by HRSEC-RID [4] and foaming properties by the Mosalux procedure [5]. RESULTS: Both inactive yeasts increased the protein and polysaccharide concentration of the base wines in comparison with the non-supplemented control wines. Optiwhite was more effective for enriching polysaccharide concentration whereas Optimumwhite was more effective for enriching protein concentration. Regardless the foam properties, supplementation with Optiwhite originated base wines with a significant higher value of foamability (Hm) than the control. The persistence of the foam (Hs) also tended to be higher but this increase was not statistically significant. Optimumwhite also tended to increase both parameters (Hm and Hs) but none of these differences was statistically significant. CONCLUSIONS: The supplementation with inactive yeasts is a useful tool to increase protein and polysaccharide concentration of base wines and also to improve its foam properties.

REFERENCES: [1] Esteruelas M, González-Royo E, Kontoudakis N, Orte A, Cantos A, Canals JM, Zamora F (2015) J. Sci. Food Agric., 95, 2071-2080 [2] Pozo-Bayón MA, Andujar-Ortiz I, Alcalde-Hidalgo JM, Martín-Alvárez PJ, Moreno-Arribas MV (2009) J. Agric. Food Chem. 57, 10784-10792 [3] Canals JM, Arola L, Zamora F (1998) Am. J. Enol. Vitic., 49, 383-388 [4] Ayestaran B, Guadalupe Z, Leon D (2004) Anal. Chim. Acta. 513:29-39 [5] Maujean A, Poinsaut P, Dantan H, Brissonet F, Cossiez E (1990) Bull. OIV, 711-712, 405-426

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Fernando Zamora*, Elena González-Royo, Joan Miquel Canals, José María Heras, Laura Medina, Nathalie Sieczkowski

*Universitat Rovira i Virgili

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Proteins constitute one of the three main components of grape juice and white wine, phenolic compounds and polysaccharides being the others. A specific group of the total grape-derived proteins resists degradation or adsorption during the winemaking process and remains in finished wine if not removed by the commonplace commercial practice of bentonite fining. While bentonite is effective in removing the problematic proteins, it is claimed to adversely affect the quality of the treated wine under certain conditions, through the removal of colour, flavor and texture compounds. A number of studies have indicated that different protein fractions require distinct bentonite concentrations for protein removal and consequent heat stabilization.

Colour assessment of port wines using colorimetric and spectrophotometric methods

Colour is an important quality parameter in wines and is the result of a complex mixture of pigments
(including anthocyanins and their derivatives, quinones, xanthyllium compounds, etc.). Red wine colour changes over time as pigments react between themselves and with other wine macromolecules
(particularly polyphenols). During wine tasting, colour is normally assessed on the outer rim of the wine profile in a tilted glass, since most wines are too opaque to be analysed in the middle of the glass. Therefore, depending on the depth of observation considered, the perception of wine colour can be different.

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Fractionation of copper and iron in wine: Assessment of potential macromolecule and sulfur binding agents

Copper and iron are known to substantially impact wine stability through oxidative, reductive or colloidal phenomena. However, the binding of metal ions to different wine components under wine conditions, and the impact of this binding on the ability of the metal ions to induce spoilage processes, is not well understood. This study surveyed a range of red and white wines for an understanding of the variability of broad metal categories within the wines. The techniques utilized included an electrochemical constant current stripping potentiometry technique (ccSP), and solid phase extraction (SPE) fractionation of wine with subsequent analysis of the metal content of each fraction by inductively coupled plasma – optical emission spectroscopy (ICP-OES).

Impact of non-fruity compounds on red wines fruity aromatic expression: the role of higher alcohols

A part, at least, of the fruity aroma of red wines is the consequence of perceptive interactions between various aromatic compounds, particularly ethyl esters and acetates, which may contribute to the perception of fruity aromas, specifically thanks to synergistic effects.1,2 The question of the indirect impact of non-fruity compounds on this particular aromatic expression has not yet been widely investigated. Among these compounds higher alcohols (HA) represent the main group, from a quantitative standpoint, of volatiles in many alcoholic beverages. Moreover, some bibliographic data suggested their contribution to the aromatic complexity by either increasing or masking flavors of wine, depending of their concentrations.