Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

Abstract

Foam stability of sparkling wines is significantly favored by the presence of surface active agents such as proteins and polysaccharides [1]. For that reason, the renowned sparkling wines are aged after the second fermentation in contact with the lees for several months (even years). Thereby wines are enriched in these macromolecules due to yeast autolysis. Since this practice is slow and costly, winemakers are seeking for alternative procedures to increase their concentration in base wines. In that sense, the supplementation with inactive yeast during alcoholic fermentation has been proposed [2]. The aim of this study was to determine whether this new strategy is really useful for enriching base wines in macromolecules and for improving foam properties of the base wines. MATERIAL AND METHODS: Macabeo grapes were harvested at the appropriate maturity and pressed. The grape juice was immediately sulfited and pectinolytic enzymes were added to facilitate settling. After 24 h, clean grape juices (around 70 L each) were racked into nine 100-L stainless steel tanks and were fermented under controlled temperature (16-18 ºC) with selected yeasts. Three tanks were considered as controls whereas other 6 were supplemented with 40 g/hL of 2 inactive yeasts (3 with Optiwhite and 3 with Optimumwhite; Lallemand Inc., Montreal, Canada). Once the alcoholic fermentation was finished, wines were sulfited, racked and cold stabilized. Proteins were analyzed by HRSEC-DAD [3], polysaccharides by HRSEC-RID [4] and foaming properties by the Mosalux procedure [5]. RESULTS: Both inactive yeasts increased the protein and polysaccharide concentration of the base wines in comparison with the non-supplemented control wines. Optiwhite was more effective for enriching polysaccharide concentration whereas Optimumwhite was more effective for enriching protein concentration. Regardless the foam properties, supplementation with Optiwhite originated base wines with a significant higher value of foamability (Hm) than the control. The persistence of the foam (Hs) also tended to be higher but this increase was not statistically significant. Optimumwhite also tended to increase both parameters (Hm and Hs) but none of these differences was statistically significant. CONCLUSIONS: The supplementation with inactive yeasts is a useful tool to increase protein and polysaccharide concentration of base wines and also to improve its foam properties.

REFERENCES: [1] Esteruelas M, González-Royo E, Kontoudakis N, Orte A, Cantos A, Canals JM, Zamora F (2015) J. Sci. Food Agric., 95, 2071-2080 [2] Pozo-Bayón MA, Andujar-Ortiz I, Alcalde-Hidalgo JM, Martín-Alvárez PJ, Moreno-Arribas MV (2009) J. Agric. Food Chem. 57, 10784-10792 [3] Canals JM, Arola L, Zamora F (1998) Am. J. Enol. Vitic., 49, 383-388 [4] Ayestaran B, Guadalupe Z, Leon D (2004) Anal. Chim. Acta. 513:29-39 [5] Maujean A, Poinsaut P, Dantan H, Brissonet F, Cossiez E (1990) Bull. OIV, 711-712, 405-426

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Fernando Zamora*, Elena González-Royo, Joan Miquel Canals, José María Heras, Laura Medina, Nathalie Sieczkowski

*Universitat Rovira i Virgili

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Impact of non-fruity compounds on red wines fruity aromatic expression: the role of higher alcohols

A part, at least, of the fruity aroma of red wines is the consequence of perceptive interactions between various aromatic compounds, particularly ethyl esters and acetates, which may contribute to the perception of fruity aromas, specifically thanks to synergistic effects.1,2 The question of the indirect impact of non-fruity compounds on this particular aromatic expression has not yet been widely investigated. Among these compounds higher alcohols (HA) represent the main group, from a quantitative standpoint, of volatiles in many alcoholic beverages. Moreover, some bibliographic data suggested their contribution to the aromatic complexity by either increasing or masking flavors of wine, depending of their concentrations.

Attractiveness and sweetness of red wines: Synergies between American oak barrels and mannoproteins

In partnership with a Bordeaux property wanting to improve the quality of its second wine, the effects of two factors, American oak barrels and mannoproteins were studied. Their impact on the attractiveness and sweetness of wines were characterized during two successive vintages (2012 and 2013). Vinification took place with a homogeneous batch of Cabernet Sauvignon. The wine was then divided up into various groups of five barrels of French and American oak, new or reused. Analyses of volatile and non-volatile wood compounds were undertaken at four months and eight months of wood ageing, by LC-MS and GC-MS.

Characterization of Glycosidically Bound Aroma Compounds of País cv. grapes of different Chilean zones

País grape has been estimated to arrive to Chile almost 500 years ago, being the first strain grown in this country. Traditionally, this grape has been used to mix with other varieties, to produce poor quality wines, but today is beginning to be used in the production of high quality wines. However, very little is known about the chemical characteristics of this variety. The aroma is one of the most important quality attributes of wine. Volatile compounds of this beverage may come from the grape (varietal aromas), from the fermentation process, from the ageing. The aromatic compounds are found in the grape in two forms: as free volatile compounds and as non-volatile compounds. The last ones, are aroma precursors present mainly as glycoconjugates formed by a sugar and an aglycone…

Multivariate strategies for red wines classification using stilbenes and flavonols content

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C).

Impact of heating must before fermentation on Chardonnay wines

Prefermentation steps of white winemaking are very important for controlling the stability and the sensory attributes of wines. Usually musts are clarified by cold settling to prevent the start of the fermentation, before racking big lees and thus limiting the appearance of vegetable or reduction off flavour while favouring an aromatic expression with low turbidity. Besides, to reach the protein stability, some white wines further require a bentonite fining, sometimes associated with negative effects on the sensory quality. This study aims to know the impact of musts heating after pressing on a Chardonnay wine in northern conditions by comparison with a classic cold racking of the must.