terclim by ICS banner
IVES 9 IVES Conference Series 9 Open-GPB 9 Open-GPB-2024 9 Flash - Abiotic interactions 9 From genes to vineyards: system biology and new breeding technologies for water stress tolerance in grapevines

From genes to vineyards: system biology and new breeding technologies for water stress tolerance in grapevines

Abstract

One of the major challenges for food security and sovereignty is to produce stress-tolerant plants without introducing foreign DNA, because the legislative process, that bans transgenics, challenges us to find new solutions for producing plants that can survive the drought. To achieve this goal, we need to identify genes that can be modified to improve stress tolerance in plants. In this work, we present an online tool for exploring the transcriptome of grapevines under water stress, which is one of the most important abiotic stresses affecting viticulture. The tool is based on a comprehensive collection of rna-seq data from 997 experiments, covering four different tissues (leaf, root, berry, and shoot), various levels of water stress, and diverse genetic backgrounds (cultivars and rootstocks) with different levels of tolerance to water stress. The tool will be integrated within VitViz and Grapedia, two web portals that provide access to genomic data of grapevine. The tool allows us to compare the expression of all grapevine genes, using the V3 genome of ‘PN40024’ as a reference. With this app, we discovered a couple of genes that could boost the drought tolerance of grapevines by cis/trans-genesis (such as the raffinose synthase and AQUILO) or by genome editing (GRETCHEN HAGEN gene). We applied these two methods to different rootstocks and cultivars of grapevines.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Álvaro Vidal Valenzuela1,2,3,4*, José Tomás Matus2,Antonio Santiago Pajuelo2, Maria Stella Grando4, Felipe Gainza-Cortés3, Lorenza Dalla Costa1, Mickael Malnoy1

1 Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38098 San michelle all’adige(TN), Italy
2 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
3 Center for Research and Innovation (CII), Viña Concha y Toro, 3550000 , Pencahue, Chile
4 Center Agriculture Food Environment (C3A), University of Trento, via E. Mach 1, 38010 San Michele all’Adige, Italy

Contact the author*

Keywords

Transcriptome, Abiotic stress, Drought, Rna-seq, Bioinformatics

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Understanding the genetic determinism of phenological and quality traits in ‘Corvina’ grape variety for selection of improved genotypes

Downy and powdery mildew are major issues in grapevine cultivation, requiring many phytosanitary treatments to ensure yield and quality. Climatic changes are also challenging grape cultivation

Controlling Wine Oxidation: Effects of pH on Key Reaction Rates

Acidity is often touted as a predictor of wine ageability, though surprisingly few studies have systematically investigated the chemical basis for this claim.

GRAPE SPIRITS FOR PORT WINE PRODUCTION: SCREENING THEIR AROMA PROFILE

Port is a fortified wine, produced from grapes grown in the demarcated Douro region. The fortification process consists in the addition of a grape spirit (77% v/v) to the fermenting juice for fermentation interruption, resulting in remaining residual sugars in the wine and increased alcohol content (19-22%). The approval of grape spirits follows the Appellation (D.O. Port wine) rules1 and it is currently carried out based on analytical control and on sensory evaluation done by the public Institute that upholds the control of the quality of Douro Appellation wines. However, the producers of Port wines would like to have more information about quality markers of grape spirits.

Scientific research for an «Ad Maiora 4.1C» application «A step back towards the future universally sustainable EME4.1C». A concrete example of forward-looking and revolutionary entrepreneurial choices in the vine and wine sector

In 1979 an enlightened and farsighted business owner in an area and in an activity unknown to him and in 120 hectares of land cultivated with corn and wheat expressed to one of us that he wanted to start a business in the wine sector. The first innovative “Vigna Dogarina Scientific Applicative Project” has become famous and harmoniously inserted in and with the “Territoir” of eastern Veneto in northeastern Italy. The revolutionary project allowed one of us: 1. to put into practice results of research related to the applied philosophy, vision, methodology of the “Great MetaEthic Chain 4.1C®” algorithm of the “Conegliano Campus 5.1C®” that considers all material, immaterial, spiritual, technical, economic, environmental, social, existential, relational, ethical, MetaEthical factors with basic indexing in a harmonious chain “ 4.1C®” and application “5.1C®”, 2. to implement:

Revealing the aroma profile of Greek wines from indigenous grape cultivars

The indigenous Greek grape varieties Assyrtiko, Malagousia, Moschofilero and Roditis are used to produce white wines that are attracting the interest of wine producers and consumers due to their aromatic characteristics [1]. In addition, the Agiorgitiko and Xinomavro varieties are Greece’s most prominent red grape varieties.