terclim by ICS banner
IVES 9 IVES Conference Series 9 Raffinose: a sweet solution for grapevine drought tolerance

Raffinose: a sweet solution for grapevine drought tolerance

Abstract

Water tolerance in plants is often associated with the accumulation of osmotic protectants, which are secondary metabolites that can help the plant to cope with water stress. One of the key osmotic protectants is a sugar called Raffinose, which is synthesized by a family of enzymes called Raffinose synthases. In this work, we focused on one of these enzymes, VviRAF2, which is a gene that shows different expression levels and genetic variants (SNPs) among different grapevine cultivars, ranging from tolerant to susceptible to water stress, and the transcription factors that may regulate the expression of this gene family. We analyzed the transcriptome data of these cultivars and constructed a gene co-expression network based on the reference genome, which revealed the involvement of the MYB transcription factor named ‘AQUILO’. To test the function of VviRAF2 and ‘AQUILO’ in water-stress tolerance, we engineered such genes via Agrobacterium tumefaciens using both, transgenic and cisgenic approach: one VviRAF2 under the control of the 35-s promoter, and another with the insertion of AQUILO controlled by its own promoter. During this study, we performed gene expression experiments on transformed lines to compare the DEGs in response to water-stress. Finally, we present the preliminary results related to stress response underlying the pathways of water stress tolerance.  

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Álvaro Vidal Valenzuela1,2,3,4*, José Tomás Matus2, David Navarro-Paya2,Felipe Gainza-Cortés3, Maria Stella Grando4, Olivier Zekri5, Pierre Videau5, Katerina Labonova5, Lorenza Dalla Costa1, Mickael Malnoy1

1 Research and Innovation centre, Fondazione Edmund Mach, Via Mach 1, 38098 San michelle all’adige(TN), Italy
2 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
3 Center for Research and Innovation (CII), Viña Concha y Toro, 3550000, Pencahue, Chile
4 Center Agriculture Food Environment (C3A), University of Trento, via E Mach 1, 38010 San Michele all’Adige, Italy
5 Mercier Novatech, Le Champ des Noëls, 85770 – Le gué de velluire, France

Contact the author*

Keywords

Raffinose, Metabolites, Drought, Abiotic stress, sugar

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Investigating the impact of grape exposure and UV radiations on rotundone in Vitis vinifera L. Tardif grapes under field trial conditions

Rotundone is the main aroma compound responsible for peppery notes in wines whose biosynthesis is negatively affected by heat and drought. Through the alteration of precipitation regime and the increase in temperature during maturation, climate change is expected to affect wine peppery typicality. In this context there is a demand for developing sustainable viticultural strategies to enhance rotundone accumulation or limit its degradation. It was recently proposed that ultraviolet (UV) radiations could stimulate rotundone production. The aim of this study was to investigate under field trial conditions the impact of grape exposure and UV treatments on rotundone in Vitis vinifera L. Tardif, an almost extinct grape variety from south-west France that can express particularly high rotundone levels. Four different treatments were compared in 2021 to a control treatment using a randomised complete block design with three replications per treatment. Grape exposure was manipulated through early or late defoliation. Leaf and laterals shoots were removed at Eichorn Lorenz growth stages 32 or 34 on the morning-sun side of the canopy. During grape maturation, UV radiations were either reduced by 99% by installing UV radiation-shielding sheets, or applied four times using the Boxilumix™ non thermal device (Asclepios Tech, Tournefeuille) with the aim of activating plant signalling pathway. Loggers displayed in solar radiation shields were used to assess the effect of such shielding sheets on air temperature within the bunch zone. The composition of grapes subjected to these treatments will be soon analysed for their rotundone content and basic classical laboratory analyses. Grapes will be harvested to elaborate wines under standardized small-scale vinification conditions (60kg) that will be assessed by a trained sensory panel.

What about oxygen transfer during wine aging in barrels?

During wine aging, several complex phenomena of gas transfer take place in barrels due to the wine/oak contact. The efficiency of this gas transfer varies according to oak wood’s intrinsic physical properties. This research aims to better understand oxygen transfer phenomena through dry oak staves and especially through stave gaps, in order to reevaluate the importance of barrel-making on a barrel’s supply of oxygen. Experimentation was based on the development of an innovative permeameter of laboratory scale, for which the principal operating conditions concerning applied pressure, the choice of liquid phase/gas phase, and the grain type of oak are taken into account and investigated. With a specially developed tightening system, the existing pressure at stave gaps in a barrel could be reproduced on a laboratory scale in order to estimate its influence on oxygen transfer efficiency.

Impact of changing climatic factors on physiological and vegetative growth

Scientific information on grapevine response to predicted levels of climate parameters is scarce and not sufficient to properly position the Wine Industry for the future. It is critical that the combined effects of increased temperature and CO2 on grapevines should be examined, without omitting the important link to soil water conditions. The purpose of this study is to quantify the effects of envisioned changes in climatic parameters on the functioning and growth of young grafted grapevines under controlled conditions, simulating expected future climate changes. Scientific knowledge of precisely how the newly-planted grapevine will react morphologically, anatomically and physiologically (at leaf, root and whole plant level) to the expected changes in important climatic parameters will enable producers to make better-informed decisions regarding terroir, cultivar and rootstock choices as well as the adaptation of current cultivation practices.

The effect of different irrigation regimes on the indigenous Cypriot grape variety Xynisteri and comparison to Sauvignon blanc

Aims: The aims of this study were to (1) assess the response of the indigenous Cypriot variety Xynisteri to different irrigation regimes and (2) compare the performance of Xynisteri to Sauvignon Blanc grown in pots with different irrigation regimes.

Influence of the different cork stoppers and sulfur dose in champagne quality

As is well known, Champagne is a product of the highest quality recognized in the international market. Champagne is a type of sparkling wine made in the Champagne region