terclim by ICS banner
IVES 9 IVES Conference Series 9 Raffinose: a sweet solution for grapevine drought tolerance

Raffinose: a sweet solution for grapevine drought tolerance

Abstract

Water tolerance in plants is often associated with the accumulation of osmotic protectants, which are secondary metabolites that can help the plant to cope with water stress. One of the key osmotic protectants is a sugar called Raffinose, which is synthesized by a family of enzymes called Raffinose synthases. In this work, we focused on one of these enzymes, VviRAF2, which is a gene that shows different expression levels and genetic variants (SNPs) among different grapevine cultivars, ranging from tolerant to susceptible to water stress, and the transcription factors that may regulate the expression of this gene family. We analyzed the transcriptome data of these cultivars and constructed a gene co-expression network based on the reference genome, which revealed the involvement of the MYB transcription factor named ‘AQUILO’. To test the function of VviRAF2 and ‘AQUILO’ in water-stress tolerance, we engineered such genes via Agrobacterium tumefaciens using both, transgenic and cisgenic approach: one VviRAF2 under the control of the 35-s promoter, and another with the insertion of AQUILO controlled by its own promoter. During this study, we performed gene expression experiments on transformed lines to compare the DEGs in response to water-stress. Finally, we present the preliminary results related to stress response underlying the pathways of water stress tolerance.  

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Álvaro Vidal Valenzuela1,2,3,4*, José Tomás Matus2, David Navarro-Paya2,Felipe Gainza-Cortés3, Maria Stella Grando4, Olivier Zekri5, Pierre Videau5, Katerina Labonova5, Lorenza Dalla Costa1, Mickael Malnoy1

1 Research and Innovation centre, Fondazione Edmund Mach, Via Mach 1, 38098 San michelle all’adige(TN), Italy
2 Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Paterna, 46980, Valencia, Spain
3 Center for Research and Innovation (CII), Viña Concha y Toro, 3550000, Pencahue, Chile
4 Center Agriculture Food Environment (C3A), University of Trento, via E Mach 1, 38010 San Michele all’Adige, Italy
5 Mercier Novatech, Le Champ des Noëls, 85770 – Le gué de velluire, France

Contact the author*

Keywords

Raffinose, Metabolites, Drought, Abiotic stress, sugar

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Come proteggere un territorio viticolo: il punto di vista del giurista

La valanga di fango che si è abbattuta nel Salemitano e nell’Avellinese, provocando decine di vittime, è stata causata in larga misura dalle insufficienti opere idrauliche e dalla manca­ta manutenzione di antiquati canali idrici.

Influence of different environments on grape phenolic and aromatic composition of threeclone of ‘nebbiolo’ (Vitis Vinifera L.)

The interaction between cultivar and growing environment is the base of wine quality and typicality. In recent time the behaviour of different clones within the same cultivar became another fundamental factor influencing the enological result. In order to clarify cultivar/clone/environment relations, a trial was carried out in 2008 studying the performances of three clones of ‘Nebbiolo’, grown in different environments: south-east Piedmont (hilly and characterized by a loamy and alkaline soil) and north-east Piedmont (a plain area characterized by a sandy and acidic soil).

Relationship between chemical parameters of tannins and in-mouth attributes of grape phenolic fractions

Establish relationships between taste and mouthfeel properties of grapes and tannin-related chemical parameters. Tempranillo Tinto and Garnacha Tinta grapes were harvested from distinct blocks in different dates; each sample collection date was separated by one week. Grapes were destemmed and macerated in 15% of ethanol for one week. The polyphenolic fraction (PF) of samples was submitted to solid phase extraction on C18 cartridges and recovered with ethanol. PFs were reconstituted in wine model and their taste and mouthfeel properties were characterised by rate-K-attributes methodology. In parallel, concentration (TC) and activity (TAc) of tannins as well as the concentration of tannins linked to anthocyanins (T-A) were determined using HPLC-UV–VIS.

Variety “Rebula” (Vitis vinifera L.) determines the terroir Goriška brda “Collio” in Slovenia

A «terroir» is a group of vineyards from the same region, belonging to a specific appellation, and sharing the same type of soil, weather conditions, grapes and wine making savoir-faire, which contribute its specific personality to the wine. White wine variety «Rebula» or «Ribolla gialla» is a local and traditional variety, which is mentioned already in XIII. century like variety for tax paying and merchandise.

Optimizing disease management in the Rioja wine region: a study on Erisiphe necator and the Gubler-Thomas model

Erisiphe necator is endemic in the Rioja Appellation of Origin. Vine growers exert significant effort to protect their crops, given the economic losses this disease causes. Different studies have shown that using Gubler-Thomas Model (GTM) can reduce treatments by up to 20% compared to a full-time protection strategy. This reduction is achieved by optimizing applications based on temperature variations in late spring and summer when the disease’s conidial stage is active.