terclim by ICS banner
IVES 9 IVES Conference Series 9 Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Plant regeneration via somatic embryogenesis and preliminary trials for the application of the DNA-free genome editing in grapevine cv. Corvina veronese

Abstract

Grapevine (Vitis spp.) is a globally significant fruit crop, and enhancing its agronomic and oenological traits is crucial to meet changing agricultural conditions and consumer demands. Conventional breeding has played a key role in domesticating grapevine varieties, but it is a time-consuming process to develop new cultivars with desirable traits for cultivation.
New plant breeding techniques (NpBTs) offer a potential revolution in grapevine cultivation, and genome editing has shown promise for targeted mutagenesis. The success of these biotechnological approaches relies on efficient in vitroregeneration protocols, particularly through somatic embryogenesis (SE). This method has proven successful in some Vitis species, but its effectiveness varies due to the genotype-dependent nature of many cultivars. Moreover, protoplasts have proven to be particularly suitable for genome editing applications, but protoplasts regeneration remains generally considered inefficient in grapevine.
The focus of this study is to enhance in vitro plant regeneration protocols via SE and isolate and regenerate plants from protoplasts derived from embryogenic calli of the Corvina veronese, a variety economically important in Veneto region. Protoplasts will serve as a platform for DNA-free genome editing using CRISPR/Cas9 to target genes responsible for grapevine susceptibility to powdery and downy mildew. The study includes a preliminary phenotypic characterization of regenerated plants to assess whether gene editing or the regeneration process has influenced their morphology and behaviour compared to plants grown under standard conditions. This research aims to accelerate the development of grapevine varieties with improved traits, addressing the challenges posed by conventional breeding methods.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Clarissa Ciffolillo1*, Edoardo Bertini2, Stefania Zattoni1, Sara Lissandrini1, Sara Zenoni1, Giovanni Battista Tornielli1,3

1Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy
2Edivite s.r.l. San Pietro Viminario, Quartiere San Mauro 30, 35020 Padova, Italy
3Department of Agronomy, Food, Natural resources, Animals and Environment, University of Padova, Viale dell’Università 16, 35020 Legnaro (PD), Italy.

Contact the author*

Keywords

Vitis vinifera, Corvina veronese, Somatic embryogenesis, Protoplasts, DNA-free genome editing

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Vineyard soils and landscapes of the Burgundy Côte (France): a historical construction worth preserving

The construction of vineyard landscapes along the Burgundy Côte is the result of geological processes and of human labour. Substratum diversity in this vineyard is the result of a very long history explained by the diversity of Jurassic sedimentary facies and Tertiary tectonic activity. The nature and thickness of Quaternary deposits (Weichselian scree debris and alluvial fans) reflect sediment dynamics concurrent with the last glaciation.

Machines and fire: developing a rapid detection system for grapevine smoke contamination using NIR spectroscopy and machine learning modelling

Bushfires are a common occurrence throughout Australia and their incidence is predicted to both rise and increase in severity due to climate change. Many of these bushfires occur in areas close to wine regions, which receive different levels of exposure to smoke. Wine produced from smoke-affected grapes are characterised by unpalatable smoky aromas such as “burning rubber”, “smoked meats” and “burnt wood”. These smoke tainted wines are unprofitable and result in significant financial losses for winegrowers.

Terroir influence on growth, grapes and grenache wines in the AOC priorat, northeast Spain

The Mediterranean climate of The Priorat AOC, situated behind the coastal mountain range of Tarragona, tends towards continentality with very little precipitation during the vegetation cycle. The soil is poor, dry and rocky, largely composed of slate schist, known as “llicorella”. Vines primarily grow on steep slopes and terraces.

Thermal conditions during the grape ripening period in viticulture geoclimate. Cool night index and thermal amplitude

Le régime thermique en période de maturation du raisin est l’une des variables déterminantes de la coloration du raisin et de la richesse en arômes, anthocyanes et polyphénols des vins.

PIWIs’ variation in drought response under semi-controlled conditions 

Grapevine interspecific hybrids (PIWIs, from German “pilzwiderstandsfähige Rebsorten” meaning fungus tolerant grapevine cultivars), offer a promising alternative to traditional cultivars in many wine regions due to their tolerance to certain fungal diseases. This makes them a potential solution for sustainable wine production, particularly under organic systems. Despite extensive research on certain agronomic traits and susceptibility to biotic diseases, such as powdery and downy mildews, the response of these cultivars to abiotic stressors, such as drought, remains unclear. Our study aims to investigate the eco-physiological traits of two commercial PIWI cultivars, Muscaris and Souvignier gris, at the leaf level to evaluate their response to drought stress.