Next‑generation genome editing and breeding genomics in Vitis vinifera and Malus × domestica

Abstract

The accelerating effects of climate change on perennial crops demand transformative approaches in plant biotechnology, particularly for grapevine (Vitis vinifera), a species historically constrained by limited genetic improvement and low transformation efficiency. Here, we present the newly established Breeding Genomics Group at the Laimburg Research Centre, focused on developing next‑generation genome editing platforms for Vitis and Malus. Our work pioneers innovative CRISPR‑based strategies aimed at generating transgene‑free, precisely edited grapevine plants using single‑cell systems and ribonucleoprotein (RNP) delivery.

By combining protoplast‑based editing with advanced regeneration protocols, we aim to overcome current bottlenecks in grapevine transformation and enable efficient, DNA‑free genome editing. These platforms are integrated with multi‑omics technologies, including transcriptomics and genome annotation, to identify key regulators of stress resilience and developmental traits. Additionally, we are establishing robust in vitro systems to support high‑throughput screening and functional validation of candidate genes.

Through interdisciplinary research and international collaboration, this initiative seeks to position grapevine as a model for cutting‑edge plant biotechnology, accelerating the development of climate‑resilient cultivars adapted to Alpine and global viticultural systems.

References

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Malnoy, M. et al. (2016). DNA‑free genetically edited grapevine and apple protoplasts using CRISPR/Cas9 ribonucleoproteins. Frontiers in Plant Science, 7, 1904. 

Wang, Z. et al. (2021). Advances in grapevine genetic transformation and genome editing. Plant Cell Reports, 40, 1063–1081.