Macrowine 2021
IVES 9 IVES Conference Series 9 Evolution of flavonols during Merlot winemaking processes

Evolution of flavonols during Merlot winemaking processes

Abstract

Aim: The phenomenon of quercetin precipitation in wine (flanovol haze), has been manifested for many years in several wine-producing regions, such as Italy, Australia, and New Zealand (Gambuti et al., 2020; Lanati, Marchi, & Cascio, 2014; Somers & Ziemelis, 1985). Due to the limited information related to the quercetin aglycone behavior and its precursors during wine production in New Zealand, this study aims to monitor the evolution of flavonols and other polyphenols during the commercial fermentation of Merlot grapes, using different fermentation conditions, and vineyard treatments.

Methods: Various trials evaluating sun exposure, winemaking practices, and winemaking process management were undertaken using Merlot grapes, commercial yeast cultures, potassium metabisulphite (20 g/hL), and nutrient supplementation with DYNASTART®-LAFFORT at 20 g/hL. Samples were taken through the winemaking stages, and the polyphenols were quantified using a reversed-phase HPLC method (Garrido-Bañuelos et al., 2019; Peng et al. 2002).

Results: Grapes with elevated amounts of flavonols glycosides produced wines with higher levels of flavonol glycosides and quercetin. Wines made from grapes with greater sun exposure ended up with more flavonol glycosides (89 mg/L) and quercetin (16 mg/L) than the wines elaborated from less exposed grapes (47 mg/L and 9.4 mg/L, respectively). Certain winemaking practices showed differences in quercetin content, for example using small fermentation (250 kg) (12 mg/L), and large fermentation (five tonnes) (28 mg/L). The data also indicates that tannins and total anthocyanins were present at 786 mg/L and 156 mg/L, respectively, for small-scale ferments, and at 888 mg/L and 363 mg/L, respectively, for large-scale ferments. In evaluating the winemaking process management, the ferment pumped over (largest fermentation volume) exhibited flavonol glycosides and quercetin at the highest concentration (91 mg/L and 20 mg/L, respectively), compared to the remaining treatments. PMS, enzyme, and PMS plus enzyme, additions lowered the concentration of the flavonols glycosides at the end of the winemaking process (37 mg/L, 42 mg/L, and 43 mg/L, respectively). It was seem that the PMS plus enzyme (15.6 mg/L) increase quecetin in wine when compared to the control, no additions, (12.6 mg/L). The wines treated with enzyme, PMS, and PMS plus enzyme, also had lower concentrations of anthocyanins (215 mg/L, 233 mg/L, and 238 mg/L, respectively) than the control (291 mg/L). 

Conclusions

The study confirmed past research on the role of sun exposure in the formation of flavonols in Merlot grapes and wines. Fermentation size can improve the extraction of polyphenols into wine, and the enzyme additions can promote the hydrolysis of flavonol glycosides. In considering winemaking practices to lower flavonol content, the impact on remaining wine phenolics, of importance to wine colour and mouthfeel, also needs to be carefully evaluated.

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Cristian Hernandez

School of Chemistry University of Auckland,Paul KILMARTIN, School of Chemistry, University of Auckland Leandro DIAS, School of Food Science, University of Auckland Gianni FLEGO, Villa Maria Estate winery Rebecca DEED, School of Biological Sciences, University of Auckland

Contact the author

Citation

Related articles…

Discrimination of monovarietal Italian red wines using derivative voltammetry

Identification of specific analytical fingerprints associated to grape variety, origin, or vintage is of great interest for wine producers, regulatory agencies, and consumers. However, assessing such varietal fingerprint is complex, time consuming, and requires expensive analytical techniques. Voltammetry is a fast, cheap, and user-friendly analytical tool that has been used to investigate and measure wine phenolics.

From a local to an international scale: sensory benchmarking of PDO wines. Quincy and Reuilly PDO wines (Sauvignon blanc) as a case study (France)

In a collective marketing strategy, the Protected Designation of Origin (PDO) can be used as a quality indicator. To highlight terroir specificities, it is useful to know how the wines are positioned on the local, national or international market from a sensory point of view. This is especially true for a comparison of varietal wines (e.g. Sauvignon blanc). We focus on the case of two closed Loire Valley PDO (France): Quincy and Reuilly. Three distinct tastings were organized. Firstly, at the local level comparing the 2 PDO (11 and 9 wines, 17 professional assessors); secondly at a regional level adding 3 closed PDO: Menetou-Salon, Sancerre and Pouilly-Fumé (3 wines per PDO, 16 assessors) and thirdly at an international level comparing these 5 PDO with Sauvignon Blanc wines coming from South Africa, New Zealand and Chile (1 to 3 wines per PDO, 19 assessors). All the wines were from the 2019 vintage and were considered to have a traditional elaboration process without contact with oak. A sensory descriptive analysis was performed using an aroma wheel allowing to combine a Check-All-That-Apply methodology, often used in sensory benchmarking, with a hierarchical structuration of the attributes. The aim is to facilitate data acquisition in a professional context without common training, to consider the hierarchical relationships among the attributes during the data analysis and to be able to characterize wines with a large range of sensorial variability. We use univariate, multivariate and clustering analyses. Similarities and differences between Quincy and Reuilly PDO wines and other Sauvignon blanc wines were identified. Specific attributes can distinguish the two PDO and different proximities exist with other local PDO, while clear differences were observed compared to international wines. Our study contributes to propose and discuss a method to do a wine sensory benchmarking highlighting sensory specificities linked to origin.

On sample preparation methods for fermentative beverage VOCs profiling by GCxGC-TOFMS

Study the influence of sample preparation methods on the volatile organic compounds (VOCs) profiling for fermentative beverages by GCxGC-TOFMS analysis. METHODS: Five common sample preparation methods were tested on pooled red wine, white wine, cider, and beer. Studied methods were DHS, Liquid-liquid extraction, mSBSE, SPE and SPME. VOCs were analyzed by GCxGC-TOFMS followed by data analysis with ChromaTOF. RESULTS: The volatile organic compounds (VOCs) profiling results were very dependent on the sample preparation methods.

Use of glutathione and a selected strain of metschnikowia pulcherrima as alternatives to sulphur dioxide to inhibit natural tyrosinase of grape must and prevent browning

The enzymatic browning of grape must is still a major problem in oenology today [1] being particularly serious when the grapes have been infected by grey rot [2]. Browning is an oxidation process that causes certain foods to turn brown, which often leads to them being rejected by consumers [3]. This is a particular problem in the case of wine, because grape must is very vulnerable to enzymatic browning [4].

Lamp – a modern tool for the detection of fungal infections in the vineyard

AIM: Loop-mediated isothermal amplification (LAMP) [1] is a modern technology for fast and sensitive amplification of specific DNA sequences under isothermal conditions. Its simple handling and no need for dedicated equipment together with an evaluation of the amplification event by in-tube detection make this method advantageous and economically affordable for on-site investigations in the industry.