Terroir 2004 banner
IVES 9 IVES Conference Series 9 Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Abstract

[English version below]

Le chargement et l’accumulation des sucres ainsi que la biosynthèse des phénols ont été étudiés sur la Syrah, dans le cadre d’un programme de recherche de paramètres qui permettraient de déterminer une ou plusieurs qualités de raisin en relation avec des styles de vins pour un terroir donné. La relation entre la dynamique d’accumulation des sucres et, en parallèle, la biosynthèse des phénols a été étudiée sur Syrah/99R plantée dans un vignoble situé sur la station expérimentale de ARC Infruitec-Nietvoorbij, Stellenbosch (Afrique du Sud). La cinétique des analyses a été réalisée depuis le stade post floraison (nouaison) jusqu’au stade sur-maturation. Les vignes sont conduites en Espalier (2,75m x 1,5m), les rangs sont orientés nord – sud, le vignoble est en pente orientée est. Une irrigation par micro aspersion est appliquée de la nouaison à la véraison. La hauteur de végétation est de 1,4 m, avec 3 hauteurs de fils de palissage. Les vignes sont ébourgeonnées, palissées et écimées. La dynamique d’accumulation des sucres par baie a été étudiée à l’aide d’un protocole développé par Deloire et al., 2004 (sous presse). La biosynthèse des phénols (tanins totaux et leur degré de polymérisation, proanthocyanidols et anthocyanes) a été étudiée par spectrophotométrie et HPLC pour les anthocyanes. Le sucre est utilisé comme indicateur des relations vigne – baie (relations source – puits).
Les tanins totaux (TT) de la baie sont synthétisés depuis l’anthèse jusqu’à la véraison. Leur concentration augmente durant la croissance herbacée des baies. Elle diminue en concentration de véraison à maturation, alors que durant cette période, la quantité de TT par baie est stable. Quand les sucres sont utilisés comme indicateur physiologique, il ressort clairement que la biosynthèse des anthocyanes évolue, par baie, jusqu’à ce que ces dernières atteignent un contenu en sucre de 20 – 21 °Brix. Après ce point, l’évolution des anthocyanes par baie est indépendante de l’évolution du contenu en sucre par baie, qui se fait alors essentiellement par concentration (perte d’eau de la baie). Le chargement actif des baies en sucre est dépendant de la photosynthèse des feuilles et la régulation du déchargement phloémien du sucre dans les baies semble en partie dépendante du microclimat des grappes et des baies elles mêmes. Le chargement actif en sucre de la baie n’est pas directement corrélé à son volume.

Sugar loading and phenolic accumulation in Syrah grapes were investigated as part of an elaborate study to determine parameters that would indicate high grape quality and different grape and wine styles on a particular terroir. The relationship between the dynamics of sugar loading and phenolic accumulation in the berries of a Syrah/R99 vineyard, situated at the ARC Infruitec-Nietvoorbij , in the Stellenbosch region (South Africa), was investigated from pea size stage (green berry) to late maturity. Vines were vertically trained and spaced 2.75 x 1.5 m in north-south orientated rows on a terroir with Glenrosa soil and a west-facing slope. Microsprinkler-irrigation was applied at pea berry size and at véraison stages. The 1.4 m high canopies were suckered, shoot-positioned and topped and accommodated by means of three sets of double wires. The dynamics of berry sugar loading were studied by a method from Deloire et al, 2004 (under publication), the berry phenolic composition (total tannins and polymerisation, proanthocyanidins, anthocyanins) was analysed by spectrophotometry and anthocyanins by HPLC. Sugar was used as physiological indicator of the plant-berry (source-sink) relationship and as bunch microclimatic indicator.
The total tannin (TT) component in the berry was synthesised from anthesis to véraison. The TT concentration increased during the green berry growth stages and decreased during ripening as the berry increased in volume. The TT per berry also increased during the green berry growth stages, but kept stable during ripening. When sugar content per berry is used as physiological indicator, it is clear that anthocyanin biosynthesis occurred until a specific berry sugar content, i.e. 20 – 21 0Brix, is reached. After this point, anthocyanin evolution per berry seemed independent of berry sugar evolution, which is at that time mainly due to concentration (berry water loss) than to loading. Thus, although berry sugar loading is dependent on photosynthetic activity of the leaves, the regulation of sugar phloem unloading in the berry sink seemed to be, in part, affected by the microclimate that the berry experienced. Berry sugar loading was not directly correlated with berry volume.

DOI:

Publication date: January 10, 2022

Issue: Terroir 2004

Type: Article

Authors

A. Deloire (1), E. Kraeva (1), M. Martin (2) et J.J. Hunter (3)

(1) Agro Montpellier, UMR 1083 « sciences pour l’œnologie et la viticulture », 2 place Viala, 34060 Montpellier cedex 1, France
(2) INRA, Unité expérimentale de Pech Rouge, 11430 Gruissan, France
(3) ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa

Contact the author

Keywords

Vitis vinifera, bunch, sugar, microclimate, indicator, vine functioning

Tags

IVES Conference Series | Terroir 2004

Citation

Related articles…

An analytical framework to site-specifically study climate influence on grapevine involving the functional and Bayesian exploration of farm data time series synchronized using an eGDD thermal index

Climate influence on grapevine physiology is prevalent and this influence is only expected to increase with climate change. Although governed by a general determinism, climate influence on grapevine physiology may present variations according to the terroir. In addition, these site-specific differences are likely to be enhanced when climate influence is studied using farm data. Indeed, farm data integrate additional sources of variation such as a varying representativity of the conditions actually experienced in the field. Nevertheless, there is a real challenge in valuing farm data to enable grape growers to understand their own terroir and consequently adapt their practices to the local conditions. In such a context, this article proposes a framework to site-specifically study climate influence on grapevine physiology using farm data. It focuses on improving the analysis of time series of weather data. The analytical framework includes the synchronization of time series using site-specific thermal indices computed with an original method called Extended Growing Degree Days (eGDD). Synchronized time series are then analyzed using a Bayesian functional Linear regression with Sparse Steps functions (BLiSS) in order to detect site-specific periods of strong climate influence on yield development. The article focuses on temperature and rain influence on grape yield development as a case study. It uses data from three commercial vineyards respectively situated in the Bordeaux region (France), California (USA) and Israel. For all vineyards, common periods of climate influence on yield development were found. They corresponded to already known periods, for example around veraison of the year before harvest. However, the periods differed in their precise timing (e.g. before, around or after veraison), duration and correlation direction with yield. Other periods were found for only one or two vineyards and/or were not referred to in literature, for example during the winter before harvest.

Investigating the impact of grape exposure and UV radiations on rotundone in Vitis vinifera L. Tardif grapes under field trial conditions

Rotundone is the main aroma compound responsible for peppery notes in wines whose biosynthesis is negatively affected by heat and drought. Through the alteration of precipitation regime and the increase in temperature during maturation, climate change is expected to affect wine peppery typicality. In this context there is a demand for developing sustainable viticultural strategies to enhance rotundone accumulation or limit its degradation. It was recently proposed that ultraviolet (UV) radiations could stimulate rotundone production. The aim of this study was to investigate under field trial conditions the impact of grape exposure and UV treatments on rotundone in Vitis vinifera L. Tardif, an almost extinct grape variety from south-west France that can express particularly high rotundone levels. Four different treatments were compared in 2021 to a control treatment using a randomised complete block design with three replications per treatment. Grape exposure was manipulated through early or late defoliation. Leaf and laterals shoots were removed at Eichorn Lorenz growth stages 32 or 34 on the morning-sun side of the canopy. During grape maturation, UV radiations were either reduced by 99% by installing UV radiation-shielding sheets, or applied four times using the Boxilumix™ non thermal device (Asclepios Tech, Tournefeuille) with the aim of activating plant signalling pathway. Loggers displayed in solar radiation shields were used to assess the effect of such shielding sheets on air temperature within the bunch zone. The composition of grapes subjected to these treatments will be soon analysed for their rotundone content and basic classical laboratory analyses. Grapes will be harvested to elaborate wines under standardized small-scale vinification conditions (60kg) that will be assessed by a trained sensory panel.

Grapevine varietal diversity as mitigation tool for climate change: Agronomic and oenologic potential of 14 foreign varieties grown in Languedoc region (France)

Climate change effects in Languedoc include an expected rise in temperatures, increased evapotranspiration as well as more severe and frequent climatic hazards, such as frost, drought periods and heat waves. For winegrowers theses phenomena impact both yield and quality, resulting in more frequent unbalanced wines. Research on identified mitigation tools for vineyard management is necessary to improve resilience of grapevine agrosystems. Varietal assortment is one of them. This study focuses on agronomic and oenologic potential of 14 foreign varieties grown in Languedoc French region. Fourteen grapevine varieties were monitored during 2021 from June until harvest on eight different sites, some of which occurring on more than one site adding up to 21 different modalities: 7 white varieties Alvarinho B, Assyrtiko B (2), Malvasia Istriana B, Parellada B, Verdejo B, Verdelho B, Xarello B, and 7 black varieties Saperavi N (2), Touriga nacional N, Baga N, Aleatico N, Montepulciano N (2), Primitivo N (3), Calabrese N (3). Varietals were compared through the following parameters: phenology was assessed by using the information collected in the Database Network of French Vine Conservatories (INRAE-SupAgro-IFV, 2005-2015). The number of inflorescences for shoots from secondary buds and bourillons and suckers were observed to assess post-bud break frost tolerance potential. Grapevine water status was studied through stem water potential measurement, observation of foliage symptoms of drought, and 𝛿13C on must. Frequencies and intensities of downy mildew, powdery mildew, and black rot attacks were estimated before harvest on leaves and clusters and botrytis at harvest to assess disease susceptibilities. Berry composition was monitored from end of veraison until harvest. Yield and mean bunch weight were also calculated. Varieties were then ranked on a 1-4 scale for each parameter and compared through PCA. Forty two stations of the Mediterranean basin were compared by PCA with the Multicriteria Climatic Classification indicators in order to confront the collected information during 2021 campaign to the hypothesis that plants coming from dry and hot regions are genetically adapted to such climatic conditions.

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.

What are the optimal ranges and thresholds for berry solar radiation for flavonoid biosynthesis?

In wine grape production, canopy management practices are applied to control the source-sink balance and improve the cluster microclimate to enhance berry composition. The aim of this study was to identify the optimal ranges of berry solar radiation exposure (exposure) for upregulation of flavonoid biosynthesis and thresholds for their degradation, to evaluate how canopy management practices such as leaf removal, shoot thinning, and a combination of both affect the grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) yield components, berry composition, and flavonoid profile under context of climate change. First experiment assessed changes in the grape flavonoid content driven by four degrees of exposure. In the second experiment, individual grape berries subjected to different exposures were collected from two cultivars (Cabernet Sauvignon and Petit Verdot). The third experiment consisted of an experiment with three canopy management treatments (i) LR (removal of 5 to 6 basal leaves), (ii) ST (thinned to 24 shoots per vine), and (iii) LRST (a combination of LR and ST) and an untreated control (UNT). Berry composition, flavonoid content and profiles, and 3-isobutyl 2-methoxypyrazine were monitored during berry ripening. Although increasing canopy porosity through canopy management practices can be helpful for other purposes, this may not be the case of flavonoid compounds when a certain proportion of kaempferol was achieved. Our results revealed different sensitivities to degradation within the flavonoid groups, flavonols being the only monitored group that was upregulated by solar radiation. Within different canopy management practices, the main effects were due to the ST. Under environmental conditions given in this trial, ST and LRST hastened fruit maturity; however, a clear improvement of the flavonoid compounds (i.e., greater anthocyanin) was not observed at harvest. Methoxypyrazine berry content decreased with canopy management practices studied. Although some berry traits were improved (i.e. 2.5° Brix increase in berry total soluble solids) due to canopy management practices (ST), this resulted in a four-fold increase in labor operations cost, two-fold decrease in yield with a 10-fold increase in anthocyanin production cost per hectare that should be assessed together as the climate continues to get hot.