Terroir 2004 banner
IVES 9 IVES Conference Series 9 Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Abstract

[English version below]

Le chargement et l’accumulation des sucres ainsi que la biosynthèse des phénols ont été étudiés sur la Syrah, dans le cadre d’un programme de recherche de paramètres qui permettraient de déterminer une ou plusieurs qualités de raisin en relation avec des styles de vins pour un terroir donné. La relation entre la dynamique d’accumulation des sucres et, en parallèle, la biosynthèse des phénols a été étudiée sur Syrah/99R plantée dans un vignoble situé sur la station expérimentale de ARC Infruitec-Nietvoorbij, Stellenbosch (Afrique du Sud). La cinétique des analyses a été réalisée depuis le stade post floraison (nouaison) jusqu’au stade sur-maturation. Les vignes sont conduites en Espalier (2,75m x 1,5m), les rangs sont orientés nord – sud, le vignoble est en pente orientée est. Une irrigation par micro aspersion est appliquée de la nouaison à la véraison. La hauteur de végétation est de 1,4 m, avec 3 hauteurs de fils de palissage. Les vignes sont ébourgeonnées, palissées et écimées. La dynamique d’accumulation des sucres par baie a été étudiée à l’aide d’un protocole développé par Deloire et al., 2004 (sous presse). La biosynthèse des phénols (tanins totaux et leur degré de polymérisation, proanthocyanidols et anthocyanes) a été étudiée par spectrophotométrie et HPLC pour les anthocyanes. Le sucre est utilisé comme indicateur des relations vigne – baie (relations source – puits).
Les tanins totaux (TT) de la baie sont synthétisés depuis l’anthèse jusqu’à la véraison. Leur concentration augmente durant la croissance herbacée des baies. Elle diminue en concentration de véraison à maturation, alors que durant cette période, la quantité de TT par baie est stable. Quand les sucres sont utilisés comme indicateur physiologique, il ressort clairement que la biosynthèse des anthocyanes évolue, par baie, jusqu’à ce que ces dernières atteignent un contenu en sucre de 20 – 21 °Brix. Après ce point, l’évolution des anthocyanes par baie est indépendante de l’évolution du contenu en sucre par baie, qui se fait alors essentiellement par concentration (perte d’eau de la baie). Le chargement actif des baies en sucre est dépendant de la photosynthèse des feuilles et la régulation du déchargement phloémien du sucre dans les baies semble en partie dépendante du microclimat des grappes et des baies elles mêmes. Le chargement actif en sucre de la baie n’est pas directement corrélé à son volume.

Sugar loading and phenolic accumulation in Syrah grapes were investigated as part of an elaborate study to determine parameters that would indicate high grape quality and different grape and wine styles on a particular terroir. The relationship between the dynamics of sugar loading and phenolic accumulation in the berries of a Syrah/R99 vineyard, situated at the ARC Infruitec-Nietvoorbij , in the Stellenbosch region (South Africa), was investigated from pea size stage (green berry) to late maturity. Vines were vertically trained and spaced 2.75 x 1.5 m in north-south orientated rows on a terroir with Glenrosa soil and a west-facing slope. Microsprinkler-irrigation was applied at pea berry size and at véraison stages. The 1.4 m high canopies were suckered, shoot-positioned and topped and accommodated by means of three sets of double wires. The dynamics of berry sugar loading were studied by a method from Deloire et al, 2004 (under publication), the berry phenolic composition (total tannins and polymerisation, proanthocyanidins, anthocyanins) was analysed by spectrophotometry and anthocyanins by HPLC. Sugar was used as physiological indicator of the plant-berry (source-sink) relationship and as bunch microclimatic indicator.
The total tannin (TT) component in the berry was synthesised from anthesis to véraison. The TT concentration increased during the green berry growth stages and decreased during ripening as the berry increased in volume. The TT per berry also increased during the green berry growth stages, but kept stable during ripening. When sugar content per berry is used as physiological indicator, it is clear that anthocyanin biosynthesis occurred until a specific berry sugar content, i.e. 20 – 21 0Brix, is reached. After this point, anthocyanin evolution per berry seemed independent of berry sugar evolution, which is at that time mainly due to concentration (berry water loss) than to loading. Thus, although berry sugar loading is dependent on photosynthetic activity of the leaves, the regulation of sugar phloem unloading in the berry sink seemed to be, in part, affected by the microclimate that the berry experienced. Berry sugar loading was not directly correlated with berry volume.

DOI:

Publication date: January 10, 2022

Issue: Terroir 2004

Type: Article

Authors

A. Deloire (1), E. Kraeva (1), M. Martin (2) et J.J. Hunter (3)

(1) Agro Montpellier, UMR 1083 « sciences pour l’œnologie et la viticulture », 2 place Viala, 34060 Montpellier cedex 1, France
(2) INRA, Unité expérimentale de Pech Rouge, 11430 Gruissan, France
(3) ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa

Contact the author

Keywords

Vitis vinifera, bunch, sugar, microclimate, indicator, vine functioning

Tags

IVES Conference Series | Terroir 2004

Citation

Related articles…

Drought effect on aromatic and phenolic potential of seven recovered grapevine varieties in Castilla-La Mancha region (Spain)

The effects of climate change are seriously affecting the quality of wine grapes. High temperatures and drought cause imbalances in the chemical composition of grapes. The result is overripe grapes with low acidity and high sugar content, which produce wines with excessive alcohol content, lacking in freshness and not very aromatic. As a consequence, the search of varieties with capacity of produce quality grapes in adverse climate conditions is a good alternative to preserve the sustainability of vineyards. In this work, quality parameters of seven Vitis vinifera L. cultivars (five whites and two reds) recently recovered from extinction and grown under two different hydric regimes (rainfed and irrigated) were analyzed during the 2020 vintage. At harvest time, weight of 100 berries, must physicochemical parameters (brix degree, total acidity, malic acid, pH), and carbon and oxygen isotope ratios (δ13C, δ18O) were determined. Subsequently, varietal aroma potential index (IPAv) and total polyphenol index (TPI) were analyzed. Quality parameters, IPAv and TPI, showed significant differences between varieties and water regimes. Both red varieties, Moribel and Tinto Fragoso, stood out for their high aromatic and phenolic potential, which was higher under rainfed regime. Regarding to white varieties, Montonera del Casar and Jarrosuelto stood out in terms of varietal aroma potential. Montonera del Casar high acidity in its musts and Jarrosuelto showed the highest berry weights.

Analysis of some environmental factors and cultural practices that affect the production and quality of the Manto Negro, Callet and Prensal Blanc varieties

45 non irrigated vineyards distributed in the DO (Denomination) Pla i Llevant de Mallorca and the DO Binissalem Mallorca were used to investigate the characteristics of production and quality and their relationships certain environmental factors and cultural practices. The grape varieties investigated are autochthonous to the island of Mallorca, Manto Negro and Callet as red and Prensal Blanc as white. All plants were measured for four consecutive years in the main production and quality parameters. Among the environmental factors, the type of soil has been studied, more specifically its water retention capacity, the planting density, the age of the vineyard and the level of viral infection. The presence or absence of virus seems to have no effect on any component studied in the varieties studied. For the white variety Prensal Blanc age is negatively correlated with production and the number of bunches, nevertheless it does not cause any effect on the required quality parameters. However, for the red varieties Callet and Manto Negro, the age of the plantation is the variable that best correlates with the quality parameters, therefore the old vines should be the object of preservation by the viticulturists and winemakers in order to guarantee its contribution to the quality of the wines made with these varieties.

Frost risk projections in a changing climate are highly sensitive in time and space to frost modelling approaches

Late spring frost is a major challenge for various winegrowing regions across the world, its occurrence often leading to important yield losses and/or plant failure. Despite a significant increase in minimum temperatures worldwide, the spatial and temporal evolution of spring frost risk under a warmer climate remains largely uncertain. Recent projections of spring frost risk for viticulture in Europe throughout the 21st century show that its evolution strongly depends on the model approach used to simulate budburst. Furthermore, the frost damage modelling methods used in these projections are usually not assessed through comparison to field observations and/or frost damage reports.
The present study aims at comparing frost risk projections simulated using six spring frost models based on two approaches: a) models considering a fixed damage threshold after the predicted budburst date (e.g BRIN, Smoothed-Utah, Growing Degree Days, Fenovitis) and b) models considering a dynamic frost sensitivity threshold based on the predicted grapevine winter/spring dehardening process (e.g. Ferguson model). The capability of each model to simulate an actual frost event for the Vitis vinifera cv. Chadonnay B was previously assessed by comparing simulated cold thermal stress to reports of events with frost damage in Chablis, the northernmost winegrowing region of Burgundy. Models exhibited scores of κ > 0.65 when reproducing the frost/non-frost damage years and an accuracy ranging from 0.82 to 0.90.
Spring frost risk projections throughout the 21st century were performed for all winegrowing subregions of Bourgogne-Franche-Comté under two CMIP5 concentration pathways (4.5 and 8.5) using statistically downscaled 8×8 km daily air temperature and humidity of 13 climate models. Contrasting results with region-specific spring frost risk trends were observed. Three out of five models show a decrease in the frequency of frost years across the whole study area while the other two show an increase that is more or less pronounced depending on winegrowing subregion. Our findings indicate that the lack of accuracy in grapevine budburst and dehardening models makes climate projections of spring frost risk highly uncertain for grapevine cultivation regions.

VINIoT: Precision viticulture service for SMEs based on IoT sensors network

The main innovation in the VINIoT service is the joint use of two technologies that are currently used separately: vineyard monitoring using multispectral imaging and deployed terrain sensors. One part of the system is based on the development of artificial intelligence algorithms that are feed on the images of the multispectral camera and IoT sensors, high-level information on water stress, grape ripening status and the presence of diseases. In order to obtain algorithms to determine the state of ripening of the grapes and avoid losing information due to the diversity of the grape berries, it was decided to work along the first year 2020 at berry scale in the laboratory, during the second year at the cluster scale and on the last year at plot scale. Different varieties of white and red grapes were used; in the case of Galicia we worked with the white grape variety Treixadura and the red variety Mencía. During the 2020 and 2021 campaigns, multispectral images were taken in the visible and infrared range of: 1) sets of 100 grapes classifying them by means of densimetric baths, 2) individual bunches. The images taken with the laboratory analysis of the ripening stage were correlated. Technological maturity, pH, probable degree, malic acid content, tartaric acid content and parameters for assessing phenolic maturity, IPT, anthocyanin content were determined. It has been calculated for each single image the mean value of each spectral band (only taking into account the pixels of interest) and a correlation study of these values with laboratory data has been carried out. These studies are still provisional and it will be necessary to continue with them, jointly with the training of the machine learning algorithms. Processed data will allow to determine the sensitivity of the multispectral images and select bands of interest in maturation.

Assessment of the impact of actions in the vineyard and its surrounding environment on biodiversity in Rioja Alavesa (Spain)

Traditional viticulture areas have experienced in the last decades an intensification of field practices, linked to an increased use of fertilisers and phytosanitary products, and to a more intensive mechanization and uniformization of the landscape. This change in management has sometimes led to higher rates of soil erosion andloss of soil structure, fertility decline, groundwater contamination, and to an increased pressure of pests and diseases. Additionally, intensification usually leads to a simplification of landscapes, of particular concern in prestigious wine grape regions where the economical revenue encourages the conversion of land use from natural habitats to high value wine grape production. To revert this trend, it is necessary that growers implement actions that promote biodiversity in their vineyards. The aim of this study is to assess the impact of the implementation of cover crops, vegetational corridors, dry stone walls and vineyard biodiversity hotspots estimated through the study of arthropods. The work has been carried out in four vineyards in Rioja Alavesa belonging to Ostatu winery, where these infrastructures were implemented in 2020. The presence and diversity of arthropods was studied by capturing them at different times in the season and at different distances from the infrastructure using pit-fall traps in the soil and yellow, white and blue chromatic traps at the canopy level. This is a preliminary study in which all adult insects were sorted to the taxonomic level of order and Coleoptera were classified to morphospecies. The results obtained show that there is a relationship between the basic characteristics of the vineyard and the arthropods captured, with a positive effect, although also dependent on the vineyard, of the presence of infrastructure.