Terroir 2004 banner
IVES 9 IVES Conference Series 9 Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Abstract

[English version below]

Le chargement et l’accumulation des sucres ainsi que la biosynthèse des phénols ont été étudiés sur la Syrah, dans le cadre d’un programme de recherche de paramètres qui permettraient de déterminer une ou plusieurs qualités de raisin en relation avec des styles de vins pour un terroir donné. La relation entre la dynamique d’accumulation des sucres et, en parallèle, la biosynthèse des phénols a été étudiée sur Syrah/99R plantée dans un vignoble situé sur la station expérimentale de ARC Infruitec-Nietvoorbij, Stellenbosch (Afrique du Sud). La cinétique des analyses a été réalisée depuis le stade post floraison (nouaison) jusqu’au stade sur-maturation. Les vignes sont conduites en Espalier (2,75m x 1,5m), les rangs sont orientés nord – sud, le vignoble est en pente orientée est. Une irrigation par micro aspersion est appliquée de la nouaison à la véraison. La hauteur de végétation est de 1,4 m, avec 3 hauteurs de fils de palissage. Les vignes sont ébourgeonnées, palissées et écimées. La dynamique d’accumulation des sucres par baie a été étudiée à l’aide d’un protocole développé par Deloire et al., 2004 (sous presse). La biosynthèse des phénols (tanins totaux et leur degré de polymérisation, proanthocyanidols et anthocyanes) a été étudiée par spectrophotométrie et HPLC pour les anthocyanes. Le sucre est utilisé comme indicateur des relations vigne – baie (relations source – puits).
Les tanins totaux (TT) de la baie sont synthétisés depuis l’anthèse jusqu’à la véraison. Leur concentration augmente durant la croissance herbacée des baies. Elle diminue en concentration de véraison à maturation, alors que durant cette période, la quantité de TT par baie est stable. Quand les sucres sont utilisés comme indicateur physiologique, il ressort clairement que la biosynthèse des anthocyanes évolue, par baie, jusqu’à ce que ces dernières atteignent un contenu en sucre de 20 – 21 °Brix. Après ce point, l’évolution des anthocyanes par baie est indépendante de l’évolution du contenu en sucre par baie, qui se fait alors essentiellement par concentration (perte d’eau de la baie). Le chargement actif des baies en sucre est dépendant de la photosynthèse des feuilles et la régulation du déchargement phloémien du sucre dans les baies semble en partie dépendante du microclimat des grappes et des baies elles mêmes. Le chargement actif en sucre de la baie n’est pas directement corrélé à son volume.

Sugar loading and phenolic accumulation in Syrah grapes were investigated as part of an elaborate study to determine parameters that would indicate high grape quality and different grape and wine styles on a particular terroir. The relationship between the dynamics of sugar loading and phenolic accumulation in the berries of a Syrah/R99 vineyard, situated at the ARC Infruitec-Nietvoorbij , in the Stellenbosch region (South Africa), was investigated from pea size stage (green berry) to late maturity. Vines were vertically trained and spaced 2.75 x 1.5 m in north-south orientated rows on a terroir with Glenrosa soil and a west-facing slope. Microsprinkler-irrigation was applied at pea berry size and at véraison stages. The 1.4 m high canopies were suckered, shoot-positioned and topped and accommodated by means of three sets of double wires. The dynamics of berry sugar loading were studied by a method from Deloire et al, 2004 (under publication), the berry phenolic composition (total tannins and polymerisation, proanthocyanidins, anthocyanins) was analysed by spectrophotometry and anthocyanins by HPLC. Sugar was used as physiological indicator of the plant-berry (source-sink) relationship and as bunch microclimatic indicator.
The total tannin (TT) component in the berry was synthesised from anthesis to véraison. The TT concentration increased during the green berry growth stages and decreased during ripening as the berry increased in volume. The TT per berry also increased during the green berry growth stages, but kept stable during ripening. When sugar content per berry is used as physiological indicator, it is clear that anthocyanin biosynthesis occurred until a specific berry sugar content, i.e. 20 – 21 0Brix, is reached. After this point, anthocyanin evolution per berry seemed independent of berry sugar evolution, which is at that time mainly due to concentration (berry water loss) than to loading. Thus, although berry sugar loading is dependent on photosynthetic activity of the leaves, the regulation of sugar phloem unloading in the berry sink seemed to be, in part, affected by the microclimate that the berry experienced. Berry sugar loading was not directly correlated with berry volume.

DOI:

Publication date: January 10, 2022

Issue: Terroir 2004

Type: Article

Authors

A. Deloire (1), E. Kraeva (1), M. Martin (2) et J.J. Hunter (3)

(1) Agro Montpellier, UMR 1083 « sciences pour l’œnologie et la viticulture », 2 place Viala, 34060 Montpellier cedex 1, France
(2) INRA, Unité expérimentale de Pech Rouge, 11430 Gruissan, France
(3) ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa

Contact the author

Keywords

Vitis vinifera, bunch, sugar, microclimate, indicator, vine functioning

Tags

IVES Conference Series | Terroir 2004

Citation

Related articles…

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard

VINIoT: Precision viticulture service for SMEs based on IoT sensors network

The main innovation in the VINIoT service is the joint use of two technologies that are currently used separately: vineyard monitoring using multispectral imaging and deployed terrain sensors. One part of the system is based on the development of artificial intelligence algorithms that are feed on the images of the multispectral camera and IoT sensors, high-level information on water stress, grape ripening status and the presence of diseases. In order to obtain algorithms to determine the state of ripening of the grapes and avoid losing information due to the diversity of the grape berries, it was decided to work along the first year 2020 at berry scale in the laboratory, during the second year at the cluster scale and on the last year at plot scale. Different varieties of white and red grapes were used; in the case of Galicia we worked with the white grape variety Treixadura and the red variety Mencía. During the 2020 and 2021 campaigns, multispectral images were taken in the visible and infrared range of: 1) sets of 100 grapes classifying them by means of densimetric baths, 2) individual bunches. The images taken with the laboratory analysis of the ripening stage were correlated. Technological maturity, pH, probable degree, malic acid content, tartaric acid content and parameters for assessing phenolic maturity, IPT, anthocyanin content were determined. It has been calculated for each single image the mean value of each spectral band (only taking into account the pixels of interest) and a correlation study of these values with laboratory data has been carried out. These studies are still provisional and it will be necessary to continue with them, jointly with the training of the machine learning algorithms. Processed data will allow to determine the sensitivity of the multispectral images and select bands of interest in maturation.

20-Year-Old data set: scion x rootstock x climate, relationships. Effects on phenology and sugar dynamics

Global warming is one of the biggest environmental, social, and economic threats. In the Douro Valley, change to the climate are expected in the coming years, namely an increase in average temperature and a decrease in annual precipitation. Since vine cultivation is extremely vulnerable and influenced by the climate, these changes are likely to have negative effects on the production and quality of wine.
Adaptation is a major challenge facing the viticulture sector where the choice of plant material plays an important role, particularly the rootstock as it is a driver for adaptation with a wide range of effects, the most important being phylloxera, nematode and salt, tolerance to drought and a complex set of interactions in the grafted plant.
In an experimental vineyard, established in the Douro Region in 1997, with four randomized blocs, with five varieties, Touriga Nacional, Tinta Barroca, Touriga Franca and Tinta Roriz, grafted in four rootstocks, Rupestris du Lot, R110, 196-17C, R99 and 1103P, data was collected consecutively over 20 years (2001-2020). Phenological observations were made two to three times a week, following established criteria, to determine the average dates of budbreak, flowering and veraison. During maturation, weekly berry samples were taken to study the dynamics of sugar accumulation, amongst other parameters. Climate data was collected from a weather station located near the vineyard parcel, with data classified through several climatic indices.
The results achieved show a very low coefficient of variations in the average date of the phenophases and an important contribution from the rootstock in the dynamic of the phenology, allowing a delay in the cycle of up to10-12 days for the different combinations. The Principal Component Analysis performed, evaluating trends in the physical-chemical parameters, highlighted the effect of the climate and rootstock on fruit quality by grape varieties.

‘Cabernet Sauvignon’ (Vitis vinifera L.) berry skin flavonol and anthocyanin composition is affected by trellis systems and applied water amounts

Trellis systems are selected in wine grape vineyards to mainly maximize vineyard yield and maintain berry quality. This study was conducted in 2020 and 2021 to evaluate six commonly utilized trellis systems including a vertical shoot positioning (VSP), two relaxed VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a guyot (GY), combined with three levels of irrigation regimes based on different crop evapotranspiration (ETc) replacements, including a 25% ETc, 50% ETc, and 100% ETc. The results indicated SH yielded the most fruits and accumulated the most total soluble solids (TSS) at harvest in 2020, however, it showed the lowest TSS in the second season. In 2020, SH and HQ showed higher concentrations in most of the anthocyanin derivatives compared to the VSPs. Similar comparisons were noticed in 2021 as well. SH and HQ also accumulated more flavonols in both years compared to other trellis systems. Overall, this study provides information on the efficacy of trellis systems on grapevine yield and berry flavonoid accumulation in a currently warming climate.

Grapevine yield estimation in a context of climate change: the GraY model

Grapevine yield is a key indicator to assess the impacts of climate change and the relevance of adaptation strategies in a vineyard landscape. At this scale, a yield model should use a number of parameters and input data in relation to the information available and be able to reproduce vineyard management decisions (e.g. soil and canopy management, irrigation). In this study, we used data from six experimental sites in Southern France (cv. Syrah) to calibrate a model of grapevine yield limited by water constraint (GraY). Each yield component (bud fertility, number of berries per bunch, berry weight) was calculated as a function of the soil water availability simulated by the WaLIS water balance model at critical phenological phases. The model was then evaluated in 10 grapegrowers’ plots, covering a diversity of biophysical and technical contexts (soil type, canopy size, irrigation, cover crop). We identified three critical periods for yield formation: after flowering on the previous year for the number of bunches and berries, around pre-veraison and post-veraison of the same year for mean berry weight. Yields were simulated with a model efficiency (EF) of 0.62 (NRMSE = 0.28). Bud fertility and number of berries per bunch were more accurately simulated (EF = 0.90 and 0.77, NRMSE = 0.06 and 0.10, respectively) than berry weight (EF = -0.31, NRMSE = 0.17). Model efficiency on the on-farm plots reached 0.71 (NRMSE = 0.37) simulating yields from 1 to 8 kg/plant. The GraY model is an original model estimating grapevine yield evolution on the basis of water availability under future climatic conditions.  It allows to evaluate the effects of various adaptation levers such as planting density, cover crop management, fruit/leaf ratio, shading and irrigation, in various production contexts.