Terroir 2004 banner
IVES 9 IVES Conference Series 9 Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Abstract

[English version below]

Le chargement et l’accumulation des sucres ainsi que la biosynthèse des phénols ont été étudiés sur la Syrah, dans le cadre d’un programme de recherche de paramètres qui permettraient de déterminer une ou plusieurs qualités de raisin en relation avec des styles de vins pour un terroir donné. La relation entre la dynamique d’accumulation des sucres et, en parallèle, la biosynthèse des phénols a été étudiée sur Syrah/99R plantée dans un vignoble situé sur la station expérimentale de ARC Infruitec-Nietvoorbij, Stellenbosch (Afrique du Sud). La cinétique des analyses a été réalisée depuis le stade post floraison (nouaison) jusqu’au stade sur-maturation. Les vignes sont conduites en Espalier (2,75m x 1,5m), les rangs sont orientés nord – sud, le vignoble est en pente orientée est. Une irrigation par micro aspersion est appliquée de la nouaison à la véraison. La hauteur de végétation est de 1,4 m, avec 3 hauteurs de fils de palissage. Les vignes sont ébourgeonnées, palissées et écimées. La dynamique d’accumulation des sucres par baie a été étudiée à l’aide d’un protocole développé par Deloire et al., 2004 (sous presse). La biosynthèse des phénols (tanins totaux et leur degré de polymérisation, proanthocyanidols et anthocyanes) a été étudiée par spectrophotométrie et HPLC pour les anthocyanes. Le sucre est utilisé comme indicateur des relations vigne – baie (relations source – puits).
Les tanins totaux (TT) de la baie sont synthétisés depuis l’anthèse jusqu’à la véraison. Leur concentration augmente durant la croissance herbacée des baies. Elle diminue en concentration de véraison à maturation, alors que durant cette période, la quantité de TT par baie est stable. Quand les sucres sont utilisés comme indicateur physiologique, il ressort clairement que la biosynthèse des anthocyanes évolue, par baie, jusqu’à ce que ces dernières atteignent un contenu en sucre de 20 – 21 °Brix. Après ce point, l’évolution des anthocyanes par baie est indépendante de l’évolution du contenu en sucre par baie, qui se fait alors essentiellement par concentration (perte d’eau de la baie). Le chargement actif des baies en sucre est dépendant de la photosynthèse des feuilles et la régulation du déchargement phloémien du sucre dans les baies semble en partie dépendante du microclimat des grappes et des baies elles mêmes. Le chargement actif en sucre de la baie n’est pas directement corrélé à son volume.

Sugar loading and phenolic accumulation in Syrah grapes were investigated as part of an elaborate study to determine parameters that would indicate high grape quality and different grape and wine styles on a particular terroir. The relationship between the dynamics of sugar loading and phenolic accumulation in the berries of a Syrah/R99 vineyard, situated at the ARC Infruitec-Nietvoorbij , in the Stellenbosch region (South Africa), was investigated from pea size stage (green berry) to late maturity. Vines were vertically trained and spaced 2.75 x 1.5 m in north-south orientated rows on a terroir with Glenrosa soil and a west-facing slope. Microsprinkler-irrigation was applied at pea berry size and at véraison stages. The 1.4 m high canopies were suckered, shoot-positioned and topped and accommodated by means of three sets of double wires. The dynamics of berry sugar loading were studied by a method from Deloire et al, 2004 (under publication), the berry phenolic composition (total tannins and polymerisation, proanthocyanidins, anthocyanins) was analysed by spectrophotometry and anthocyanins by HPLC. Sugar was used as physiological indicator of the plant-berry (source-sink) relationship and as bunch microclimatic indicator.
The total tannin (TT) component in the berry was synthesised from anthesis to véraison. The TT concentration increased during the green berry growth stages and decreased during ripening as the berry increased in volume. The TT per berry also increased during the green berry growth stages, but kept stable during ripening. When sugar content per berry is used as physiological indicator, it is clear that anthocyanin biosynthesis occurred until a specific berry sugar content, i.e. 20 – 21 0Brix, is reached. After this point, anthocyanin evolution per berry seemed independent of berry sugar evolution, which is at that time mainly due to concentration (berry water loss) than to loading. Thus, although berry sugar loading is dependent on photosynthetic activity of the leaves, the regulation of sugar phloem unloading in the berry sink seemed to be, in part, affected by the microclimate that the berry experienced. Berry sugar loading was not directly correlated with berry volume.

DOI:

Publication date: January 10, 2022

Issue: Terroir 2004

Type: Article

Authors

A. Deloire (1), E. Kraeva (1), M. Martin (2) et J.J. Hunter (3)

(1) Agro Montpellier, UMR 1083 « sciences pour l’œnologie et la viticulture », 2 place Viala, 34060 Montpellier cedex 1, France
(2) INRA, Unité expérimentale de Pech Rouge, 11430 Gruissan, France
(3) ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa

Contact the author

Keywords

Vitis vinifera, bunch, sugar, microclimate, indicator, vine functioning

Tags

IVES Conference Series | Terroir 2004

Citation

Related articles…

Organic recycled mulches in sustainable viticulture: assessment of spontaneous plants communities and weed coverage

In recent years, developing more efficient and sustainable viticulture management has been essential due to the impact of climate change in semiarid regions. For this reason, the use of recycled organic mulching (ROM) in the vineyard has become an interesting strategy to cope with water stress, isolated soil from extreme temperatures and improving soil humidity, control the presence of weeds and therefore reduce the inputs of herbicides and improve soil fertility. This work aimed to analyse the effect of three different organic mulches [straw (S), grape pruning debris (GPD) and spent mushroom compost (SMC)] and two traditional soil management techniques [herbicide (H) and interrow (IN)] on weed coverage and the spontaneous plant communities’ presence. Data sampling was collected throughout the vine vegetative cycle of 2021 in La Rioja, Spain. The different soil management techniques had a clear effect on weed coverage and his development during the vine vegetative cycle. SMC and H were the treatments with the highest and the lowest coverage percentage, respectively. IN had a delayed weed emergence at the beginning of the vine vegetative cycle, but finally it reached maximum values nearby SMC. GPD and S had similar effects on weed emergence, reaching 25-30% of the maximum coverage values. A total of 29 herbaceous species were identified during the vegetative cycle, some of them very isolated and occasional. Principal component analysis (PCAs) showed a good association between spontaneous species and treatments, furthermore, specific species-treatment associations were found. Moreover, three clear groups of herbaceous communities were identified by cluster analysis. This study provides interesting information about the effect of different alternative soil management on herbaceous plant coverage and weed species communities which could contribute to making more sustainable viticulture.

Co-design and evaluation of spatially explicit strategies of adaptation to climate change in a Mediterranean watershed

Climate change challenges differently wine growing systems, depending on their biophysical, sociological and economic features. Therefore, there is a need to locally design and evaluate adaptation strategies combining several technical options, and considering the local opportunities and constraints (e.g. water access, wine typicity). The case study took place in a typical and heterogeneous Mediterranean vineyard of 1,500 ha in the South of France. We developed a participatory modeling approach to (1) conceptualize local climate change issues and design spatially explicit adaptation strategies with stakeholders, (2) numerically evaluate their effects on phenology, yield and irrigation needs under the high-emissions climate change scenario RCP 8.5, and (3) collectively discuss simulation results. We organized five sets of workshops, with in-between modeling phases. A process-based model was developed that allowed to evaluate the effects of six technical options (late varieties, irrigation, water saving by reducing canopy size, adjusting cover cropping, reducing density, and shading) with various distributions in the watershed, as well as vineyard relocation. Overall, we co-designed three adaptation strategies. Delay harvest strategy with late varieties showed little effects on decreasing air temperature during ripening. Water constraint limitation strategy would compensate for production losses if disruptive adaptations (e.g. reduced density) were adopted, and more land got access to irrigation. Relocation strategy would foster high premium wine production in the constrained mountainous areas where grapevine is less impacted by climate change. This research shows that a spatial distribution of technical changes gives room for adaptation to climate change, and that the collaboration with local stakeholders is a key to the identification of relevant adaptation. Further research should explore the potential of adaptation strategies based on soil quality improvement and on water stress tolerant varieties.

Updating the Winkler index: An analysis of Cabernet sauvignon in Napa Valley’s varied and changing climate

This study aims to create an updated, agile viticultural climate index (similar to the Winkler Index) by performing in-depth analyses of current and historical data from industry partners in several major winegrowing regions. The Winkler Index was developed in the early twentieth century based on analysis of various grape-growing regions in California. The index uses heat accumulation (i.e. Growing Degree Days) throughout the growing season to determine which grape varieties are best suited to each region. As viticultural regions are increasingly subject to the complexity and uncertainty of a changing climate, a more rigorous, agile model is needed to aid grape growers in determining which cultivars to plant where. For the first phase of this study, 21 industry partners throughout Napa Valley shared historical phenology, harvest, viticultural practice, and weather data related to their Cabernet sauvignon vineyard blocks. To complement this data, berry samples were collected throughout the 2021 growing season from 50 vineyard blocks located throughout 16 American Viticultural Areas that were then analyzed for basic berry chemistry and phenolics. These blocks have been mapped using a Geographic Information System (GIS), enabling analysis of altitude, vineyard row orientation, slope, and remotely sensed climate data. Sampling sites were also chosen based on their proximity to a weather station. By analyzing historical data from industry partners and data specifically collected for this study, it is possible to identify key parameters for further analysis. Initial results indicate extreme variability at a high spatial resolution not currently accounted for in modern viticultural climate indices and suggest that viticultural practices play a major role. Using the structure of data collection and analyses developed for the first phase, this project will soon be expanded to other wine regions globally, while continuing data collection in Napa Valley.

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.

Biodiversity in the vineyard agroecosystem: exploring systemic approaches

Biodiversity conservation and restoration are essential for guarantee the provision of ecosystem services associated to vineyard agroecosystem such as climate regulation trough carbon sequestration and control of pests and diseases. Most of published research dealing with the complexity of the vineyard agroecosystems emphasizes the necessity of innovative approaches, including the integration of information at different temporal and spatial scales and development of systemic analysis based on modelling. A biodiversity survey was conducted in the Franciacorta wine-growing area (Lombardy, Italy), one of the most important Italian wine-growing regions for sparkling wine production, considering a portion of the territory of 112 ha. The area was divided into several Environmental Units (EUs), defined as a whole vineyard or portion of vineyard homogenous in terms of four agronomic characteristics: planting year, planting density, cultivar, and training system. In each EU a set of compartments was identified and characterised by specific variables. The compartments are meteorology, morphology (altitude, slope, aspect, row orientation, and solar irradiance), ecological infrastructures and management. The landscape surrounding EU was also characterised in terms of land-use in a buffer zone of 500 m. For each component a specific methodology was identified and applied. Different statistical approaches were used to evaluate the method to integrate the information related to different compartments within the EU and related to the buffer zone. These approaches were also preliminarily evaluated for their ability to describe the contribution of biodiversity and landscape components to ecosystem services. This methodological exploration provides useful indication for the development of a fully systemic approach to structural and functional biodiversity in vineyard agroecosystems, contributing to promote a multifunctional perspective for the all wine-growing sector.