Terroir 2004 banner
IVES 9 IVES Conference Series 9 Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Sugar loading and phenolic accumulation as affected by ripeness level of Syrah/R99 grapes

Abstract

[English version below]

Le chargement et l’accumulation des sucres ainsi que la biosynthèse des phénols ont été étudiés sur la Syrah, dans le cadre d’un programme de recherche de paramètres qui permettraient de déterminer une ou plusieurs qualités de raisin en relation avec des styles de vins pour un terroir donné. La relation entre la dynamique d’accumulation des sucres et, en parallèle, la biosynthèse des phénols a été étudiée sur Syrah/99R plantée dans un vignoble situé sur la station expérimentale de ARC Infruitec-Nietvoorbij, Stellenbosch (Afrique du Sud). La cinétique des analyses a été réalisée depuis le stade post floraison (nouaison) jusqu’au stade sur-maturation. Les vignes sont conduites en Espalier (2,75m x 1,5m), les rangs sont orientés nord – sud, le vignoble est en pente orientée est. Une irrigation par micro aspersion est appliquée de la nouaison à la véraison. La hauteur de végétation est de 1,4 m, avec 3 hauteurs de fils de palissage. Les vignes sont ébourgeonnées, palissées et écimées. La dynamique d’accumulation des sucres par baie a été étudiée à l’aide d’un protocole développé par Deloire et al., 2004 (sous presse). La biosynthèse des phénols (tanins totaux et leur degré de polymérisation, proanthocyanidols et anthocyanes) a été étudiée par spectrophotométrie et HPLC pour les anthocyanes. Le sucre est utilisé comme indicateur des relations vigne – baie (relations source – puits).
Les tanins totaux (TT) de la baie sont synthétisés depuis l’anthèse jusqu’à la véraison. Leur concentration augmente durant la croissance herbacée des baies. Elle diminue en concentration de véraison à maturation, alors que durant cette période, la quantité de TT par baie est stable. Quand les sucres sont utilisés comme indicateur physiologique, il ressort clairement que la biosynthèse des anthocyanes évolue, par baie, jusqu’à ce que ces dernières atteignent un contenu en sucre de 20 – 21 °Brix. Après ce point, l’évolution des anthocyanes par baie est indépendante de l’évolution du contenu en sucre par baie, qui se fait alors essentiellement par concentration (perte d’eau de la baie). Le chargement actif des baies en sucre est dépendant de la photosynthèse des feuilles et la régulation du déchargement phloémien du sucre dans les baies semble en partie dépendante du microclimat des grappes et des baies elles mêmes. Le chargement actif en sucre de la baie n’est pas directement corrélé à son volume.

Sugar loading and phenolic accumulation in Syrah grapes were investigated as part of an elaborate study to determine parameters that would indicate high grape quality and different grape and wine styles on a particular terroir. The relationship between the dynamics of sugar loading and phenolic accumulation in the berries of a Syrah/R99 vineyard, situated at the ARC Infruitec-Nietvoorbij , in the Stellenbosch region (South Africa), was investigated from pea size stage (green berry) to late maturity. Vines were vertically trained and spaced 2.75 x 1.5 m in north-south orientated rows on a terroir with Glenrosa soil and a west-facing slope. Microsprinkler-irrigation was applied at pea berry size and at véraison stages. The 1.4 m high canopies were suckered, shoot-positioned and topped and accommodated by means of three sets of double wires. The dynamics of berry sugar loading were studied by a method from Deloire et al, 2004 (under publication), the berry phenolic composition (total tannins and polymerisation, proanthocyanidins, anthocyanins) was analysed by spectrophotometry and anthocyanins by HPLC. Sugar was used as physiological indicator of the plant-berry (source-sink) relationship and as bunch microclimatic indicator.
The total tannin (TT) component in the berry was synthesised from anthesis to véraison. The TT concentration increased during the green berry growth stages and decreased during ripening as the berry increased in volume. The TT per berry also increased during the green berry growth stages, but kept stable during ripening. When sugar content per berry is used as physiological indicator, it is clear that anthocyanin biosynthesis occurred until a specific berry sugar content, i.e. 20 – 21 0Brix, is reached. After this point, anthocyanin evolution per berry seemed independent of berry sugar evolution, which is at that time mainly due to concentration (berry water loss) than to loading. Thus, although berry sugar loading is dependent on photosynthetic activity of the leaves, the regulation of sugar phloem unloading in the berry sink seemed to be, in part, affected by the microclimate that the berry experienced. Berry sugar loading was not directly correlated with berry volume.

DOI:

Publication date: January 10, 2022

Issue: Terroir 2004

Type: Article

Authors

A. Deloire (1), E. Kraeva (1), M. Martin (2) et J.J. Hunter (3)

(1) Agro Montpellier, UMR 1083 « sciences pour l’œnologie et la viticulture », 2 place Viala, 34060 Montpellier cedex 1, France
(2) INRA, Unité expérimentale de Pech Rouge, 11430 Gruissan, France
(3) ARC Infruitec-Nietvoorbij, Private Bag X5026, 7599 Stellenbosch, South Africa

Contact the author

Keywords

Vitis vinifera, bunch, sugar, microclimate, indicator, vine functioning

Tags

IVES Conference Series | Terroir 2004

Citation

Related articles…

Combining effect of leaf removal and natural shading on grape ripening under two irrigation strategies in Manto negro (Vitis vinifera L.)

The increasingly frequent heat waves during grape ripening pose challenges for high quality wine grape production. Defoliation is a common practice that can improve the control of diseases in bunches, but also it increases the exposure to sunlight. Grapes exposed to solar radiation reach temperatures over the optimum for berry development and maturation. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 using Manto negro wine grapes to study the effect of applied irrigation and different light exposure levels on grape quality. Two irrigation treatments were imposed based on the frequency and amount of water doses in a four-block experimental vineyard at Bodega Ribas (Mallorca). Three light exposure treatments were randomly applied in each irrigation plot. The light treatments included exposed clusters from pea size, non-exposed clusters, and shaded clusters after softening. Leaf area index and canopy porosity was estimated every 2 weeks. Midday leaf water potential was measured weekly. Additionally, apparent electrical conductivity was measured between rows to estimate the soil water content variability. Light and temperature sensors were installed at the bunch level to quantify the differences in bunch temperature and light intensity among treatments. The effect of irrigation and cluster light exposure on berry weight, TSS, TA, malic acid, tartaric acid, K+, and pH were analysed at 5 moments along grape ripening. During different heat waves, the natural shading technique decreased the maximum bunch temperature around 10 °C respect to the exposed bunches in both irrigation strategies. The combination of defoliation and shading techniques after softening decreased TSS at harvest and affected most of the quality parameters during the last stages of ripening, showing an interesting technique to delay ripening in warm viticulture areas.

Water deficit differentially impacts the performances and the accumulation of grape metabolites of new varieties tolerant to fungi

The use of resistant varieties is a long-term but promising solution to reduce chemical input in viticulture. Several important breeding programs in Europe and abroad are now releasing a range of new hybrids performing well regarding fungi susceptibility and producing good quality wines. Unfortunately, insufficient attention is paid by the breeders to the adaptation of these varieties to climatic changes, notably to the increased climatic demand and water deficit (WD). Thus, prior to the adoption of such varieties by the wine industry in Mediterranean regions, there is a need to consider their suitability to WD. This study aimed to characterize the different drought-strategies adopted by 6 new resistant varieties selected by INRAE in comparison to Syrah. To allow the assessment of long-term impacts of WD, field-grown vines were exposed to contrasted WD from 2018 to 2021 under a semi-arid Mediterranean climate. A gradient of WD was applied in the field and controlled through plant measurements at the single plant level. Grape development was non-destructively monitored to determine the arrest of berry phloem unloading. The impacts of WD on berry composition, including water, primary metabolites (sugars, organic acids), secondary metabolites (anthocyanins, thiols precursors) and main cations contents, were assessed at this specific stage. Results showed different varietal responses during the year and inter-annual acclimation in terms of plant water use efficiency, biomass accumulation, as well as yield components and berry composition. WD differentially reduced the accumulation of primary metabolites at plant and berry levels, but it little changed their concentrations in the fruits at the ripe stage. Moreover, WD differentially impacted the accumulation of secondary metabolites and major cations between the varieties. In the talk, we’ll present the main results regarding the WD impacts on fruit metabolites and enlarge the reflection about the practical assessment of the grapevine acclimation to WD.

Impact of changes in pruning practices on vine growth and yield

A gradual decline in vineyards has been observed over the past twenty years worldwide. This might be explained by the climate change, practices change or the increase of dieback diseases. To increase the longevity of vines, we studied the impact of different pruning strategies in four adult and four young vineyards located in France and Spain. In France, vineyards were planted with Cabernet franc on 3309C while Spanish trials were planted with Tempranillo grafted on 110R. Vegetative expression, yield, quality of berries and wood vessels conductivity were measured. The distribution of vegetative expression, yield and berry composition between primary and secondary vegetation were quantified. Finally, tomography was used to evaluate the implication of the treatments on sap flows.
First results show that i) the respectful pruning leads to an increase of 30 to 50% more secondary shoots than the aggressive pruning in France and between 15 and 20% in Spain, ii) there is no major effect on the yield over the first two years following the implementation of the new pruning practices, although the proportion of clusters from suckers is higher on the respectful pruning method. On young vines, the development of the trunk according to a respectful pruning leads to a loss of harvest 2 years after planting. This is due to the removal, on the future trunk, of the green suckers which carrying bunches. This operation carried out in spring rather than during winter pruning, would promote a better leaf / fruit balance when the plant comes into production, and could lead to better hydraulic conduction in the vessels of the trunk. Maintaining these trials for several years will provide more robust data to assess the impact of these practices on the vines over the long term.

Variety and climatic effects on quality scores in the Western US winegrowing regions

Wine quality is strongly linked to climate. Quality scores are often driven by climate variation across different winegrowing regions and years, but also influenced by other aspects of terroir, including variety. While recent work has looked at the relationship between quality scores and climate across many European regions, less work has examined New World winegrowing regions. Here we used scores from three major rating systems (Wine Advocate, Wine Enthusiast and Wine Spectator) combined with daily climate and phenology data to understand what drives variation across wine quality scores in major regions of the Western US, including regions in California, Oregon and Washington. We examined effects of variety, region, and in what phenological period climate was most predictive of quality. As in other studies, we found climate, based mainly on growing degree day (GDD) models, was generally associated with quality—with higher GDD associated with higher scores—but variety and region also had strong effects. Effects of region were generally stronger than variety. Certain varieties received the highest scores in only some areas, while other varieties (e.g., Merlot) generally scored lower across regions. Across phenological stages, GDD during budbreak was often most strongly associated with quality. Our results support other studies that warmer periods generally drive high quality wines, but highlight how much region and variety drive variation in scores outside of climate.

Downscaling of remote sensing time series: thermal zone classification approach in Gironde region

In viticulture, the challenges of local climate modelling are multiple: taking into account the local environment, fine temporal and spatial scales, reliable time series of climate data, ease of implementation and reproducibility of the method. At the local scale, recent studies have demonstrated the contribution of spatialization methods for ground-based climate observation data considering topographic factors such as altitude, slope, aspect, and geographic coordinates (Le Roux et al, 2017; De Rességuier et al, 2020). However, these studies have shown questions in terms of the reproducibility and sustainability of this type of climate study. In this context, we evaluated the potential of MODIS thermal satellite images validated with ground-based climate data (Morin et al, 2020). Previous studies have been encouraging, but questions remain to be explored at the regional scale, particularly in the dynamics of the massive use of bioclimatic indices to classify the climate of wine regions. The results at the local scale were encouraging, but this approach was tested in the current study at the regional scale. Several objectives were set: 1) to evaluate the downscaling method for land surface temperature time series, 2) to identify regional thermal structure variations. We used weekly minimum and maximum surface temperature time series acquired by MODIS satellites at a spatial resolution of 1000 m and downscaled at 500 m using topographical variables. Two types of analyses were performed: