Techniques of delimitation in France

In Burgundy, a climat has nothing to do with the weather but accurately designates a named vine plot, often centuries-old, which produces a singular wine. This wine is the combination of history, the natural environment (relief, type of soil, exposure to the sun), a grape variety and know-how going back thousands of years. The grapes of each climat are harvested separately and the wine is made from a single grape variety and has a unique name featured on the bottle. Romanée conti, clos de vougeot, montrachet, musigny, corton…

The chemical composition of grape berries at harvest is one of the most important factors that should be considered to produce high quality wines. Among the different chemical classes which characterize the grape juice, the polyphenolic compound, such as flavonoids, contribute to the final taste and color of wines. Recently, an innovative non-destructive method, based on chlorophyll fluorescence, was developed to estimate the phenolic maturity of red grape varieties through the evaluation of anthocyanins accumulated in the berry skin. To date, only few data are available about the application of this method on white grape varieties.

Conventional molecular analyses provide bulk genomic/transcriptomic data that are unable to reveal the cellular heterogeneity and to precisely define how gene networks orchestrate organ development. We will profile gene expression and identify open chromatin regions at the individual cells level, allowing to define cell-type specific regulatory elements, developmental trajectories and transcriptional networks orchestrating organ development and function. We will perform scRNA-seq and snATAC-seq on leaf/berry protoplasts and nuclei and combine them with the leaf/berry bulk tissues obtained results, where the analysis of transcripts, chromatin accessibility, histone modification and transcription factor binding sites showed that a large fraction of phenotypic variation appears to be determined by regulatory rather than coding variation and that many variants have an organ-specific effect.

Context and purpose of the study. The concept of terroir encompasses the investigation of the physical environment’s influence on grapevine physiology, grape composition, and wine quality, with an emphasis on employing viticultural zoning techniques to systematically characterize and analyze terroirs.

Dimethyl sulfide (DMS) is a volatile sulfur compound with a complex role in wine aroma, contributing both desirable and undesirable sensory characteristics depending on its concentration (1).