Tools for assessing vine nitrogen status; role of nitrogen uptake in the “terroir” effect

L’evoluzione del settore viti-enologico, supportato dalla ricerca ci propone sempre più frequentemente vini unici e inconfondibili. Il merito di ciò è da ricercare nel rapporto vitigno-territorio e dalla

Aim: Wine aromatic profile is a combination of viticulture and oenological practices and it is related to character, quality, and consumer acceptance. Based on the competition between soil capacity and canopy development, and on the potential to produce sparkling wines at Caldas, in the south region of Minas Gerais (Brazil) (21°55´S and 46°23´W, altitude 1.100m), the aim of this work was the evaluation of the development of aromas (secondary metabolites) from grapes to sparkling wines in a vine-spacing experiment and whether the distance between the vines can influence the aromatic profile of the sparkling wines (final product). 

The objective of the present work is to assess yeast effects on the development of wine varietal aroma throughout aging and on wine longevity.

Three independent experiments were carried out; two fermenting semi-synthetic musts fortified with polyphenols and aroma precursors extracted from Tempranillo (1) or Albariño (2) grapes and with synthetic precursors of polyfunctional mercaptans (PFMs), and a third in which a must, mixture of 6 different grape varieties was used. In all cases, fermentations were carried out by different Saccharomyces cerevisiae strains and one S. kudriavzevii, and the obtained wines were further submitted to anoxic accelerated aging to reproduce bottle aging. The volatile profile of the wines was analyzed using several chromatographic procedures, in order to provide a comprehensive evaluation of wine aroma. Aroma compounds analyzed included fermentation volatile metabolites, grape-derived aroma compounds including PFMs, and Strecker aldehydes (SA).

Results revealed that the effects of yeast on wine aroma throughout its self-life extend along three main axes:

1. A direct or indirect action on primary varietal aroma and on its evolution during wine
aging.

2. The direct production of SA during fermentation and/or their delayed formation by producing the required reagents (amino acids + dicarbonyls) for Strecker degradation
during anoxic aging.

3. Producing acids (leucidic, branched acids) precursors to fruity esters. More specifically, and leaving aside the infrequent de novo formation, the action of the different strains of yeast on primary varietal aroma takes four different forms:

1.- Speeding the hydrolysis of aroma precursors, which leads to early aroma formation without changing the amount of aroma formed. In the case of labile molecules, such as linalool, the enhancement of young wine aroma implies a short-living wine. 2.- Metabolizing the aroma precursor, reducing the amounts of aroma formed, which can be of advantage for negative aroma compounds, such as TDN or guaiacol; 3.- Transforming grape components into aroma precursors, increasing the amounts of aroma formed, as for ethyl cinnamate, leucidic acid or vinylphenols; 4.- Forming reactive species such as vinylphenols able to destroy varietal polyfunctional mercaptans.

Overall, it can be concluded that the yeast carrying alcoholic fermentation not only influences fermentative wine aroma but also affects to the wine varietal aroma, to its evolution during aging and to the development of oxidative off-odors

3-Isobutyl-2-methoxypyrazine (IBMP) is one of the key molecules in wine aroma with a bell pepper aroma and a very low threshold in wine, 1-6 ng/L for white wine and 10-16 ng/L in red wine1. The differences in these thresholds are likely due to IBMP-non volatile matrix interactions. It has indeed been shown that polyphenols may influence the volatility of flavor compounds2. In the present study, we focus on IBMP-polyphenols interactions in relation to volatility and sensory perception in model wine solution. Methods: 1. GC-MS Static Headspace Analysis: Samples were analyzed by Static headspace analysis with an Agilent 7890A gas chromatograph coupled to HP 5975C mass spectrometry detector (Agilent Technologies, Santa Clara, CA, USA).

No other agricultural product has a stronger relationship with the soil than wine. This study aimed to characterize the sensory profile of red wines from the Douro Demarcated Region (RDD) certified as DOC Douro, through the application of Quantitative Descriptive Analysis (QDA®) and Temporal Dominance of Sensations (TDS) sensory methods. QDA® provides a complete word description for all a product’s sensory properties. The TDS, which is relatively recent in the sensory field [1], allows to evaluation and description of the evolution of the dominant sensory perceptions during the tasting of a food product.Eighteen commercial wines from different producers were evaluated, six different samples representing each of the three sub-regions of the RDD.