terclim by ICS banner
IVES 9 IVES Conference Series 9 International Congress on Grapevine and Wine Sciences 9 2ICGWS-2023 9 Decoupling the effects of water and heat stress on Sauvignon blanc berries

Decoupling the effects of water and heat stress on Sauvignon blanc berries

Abstract

Climate changes have important consequences in viticulture, heat waves accompanied by periods of drought are encountered more and more frequently. This study aims to evaluate the single and combined effect of water deficit and high temperatures on the thiol precursors biosynthesis in Sauvignon blanc grapes. For this purpose, a protocol has been developed for the cultivation of berries on a solid substrate. The berries, collected at three different times starting from veraison and grown in vitro, were subjected to 4 different treatments: control (C), water stress (WS), heat stress (HS), combined water and heat stress (WSHS). Water stress was simulated by adding abscisic acid to the culture medium, while different temperatures, respectively 25°C and 35°C, were managed with two illuminated climatic chambers. The expression of the main genes involved in the biosynthesis of thiol precursors, together with stress marker genes, was evaluated on the berries by qRT-PCR. Moreover, thiol precursors were analysed using an UHPLC/MS method. Results highlighted variable trends in the genes encoding GSTs (glutathione-S-transferase) and GGTs (γ-glutamyl-transferase), responsible for the synthesis of precursors. In detail, the VvGST3 gene was significantly down-regulated in thermal stresses, while HS and WSHS up-regulated the expression of VvGST2, VvGST5, VvGST25 and GGTs. Furthermore, the tested stress marker genes significantly confirm the success of both thermal and water stress conditions. The analysis of thiol precursors, showed that the concentration of Cys-3SH in the two last sampling dates well correlated with the expression of the above-mentioned genes. As opposite, a poor correlation was observed for Glut-3SH. Taken together these results allowed to identify a specific effect of heat and water stress on the regulation of the thiol precursors biosynthetic pathway in Sauvignon blanc berries during maturation.  

DOI:

Publication date: October 11, 2023

Issue: ICGWS 2023

Type: Poster

Authors

Alberto Calderan1,2, Rachele Falchi2, Riccardo Braidotti2, Giorgio Alberti2, Andreja Vanzo3 and Paolo Sivilotti2

1Department of Life Sciences, University of Trieste, via Licio Giorgieri 10, 34127 Trieste, Italy
2Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Via delle     Scienze 206, 33100 Udine, Italy
3Kmetijski Istitut Slovenje (KIS), Hacquetova ulica 17, SI-1000 Ljubljana, Slovenia

Contact the author*

Keywords

water stress, heat stress, climate change, berries culture

Tags

2ICGWS | ICGWS | ICGWS 2023 | IVES Conference Series

Citation

Related articles…

Effect of irrigation in cover cropping vineyards

Cover cropping in vineyard is a sustainable and alternative soil management system to conventional tillage that is gaining more and more importance among winegrowers and is being promoted, among other organizations, by the European Union through the eco-schemes of the Common Agricultural Policy.
However, the use of cover crops in Mediterranean viticultural environments is conditioned, to a large extent, by the availability of irrigation water which, in a context of global warming like the one we are experiencing, must be adjusted to savings strategies, supplying to the vine only what it needs in each moment.

Influence of different Lachancea thermotolerans strains in wine acidity

Wine acidity is a parameter of great importance that influences different quality factors of the product such as biological stability or organoleptic characteristics. In the current context of climate change, which gives rise to wines with higher levels of ethanol and lower acidity, the biological acidification with yeast species such as Lachancea thermotolerans could be a solution.
In this work, the effect of the inoculation of different L. thermotolerans on the acidity of wine was studied.

The interplay between water deficit and nitrogen and potassium nutrition in Vitis vinifera L.

Climate change is expected to provoke an increase in the frequency and intensity of drought events and water scarcity that will have detrimental effects on photosynthesis and plant yield. To sustain an appropriate plant yield under sub-optimal conditions, a common practice is the application of high amounts of fertilizers with negative environmental consequences. The present study aims at evaluating the interplay between water and nutrient availability, namely nitrogen (N) and potassium (K), in two grapevine cultivars with a different sensitivity to water shortage stress. Two-year-old Vitis Vinifera cv. Cabernet Sauvignon and Grenache grapevine plants grafted on SO4 rootstock have been transferred in pots under semi-environmental conditions.

Wine without added SO2: Oxygen impact and color evolution during red wine aging

SO2 play a major role in wine stability and evolution during its aging and storage. Winemaking without SO2 is a big challenge for the winemakers since the lack of SO2 affects directly the wine chemical evolution such as the aromas compounds as well as the phenolic compounds. During the red wine aging, phenolic compounds such as anthocyanin, responsible of the red wine colour, and tannins, responsible of the mouthfeel organoleptic properties of wine, evolved quickly from the winemaking process to aging [1]. A lot of new interaction and molecules occurred lead by oxygen[2] thus the lack of SO2 will induce wine properties changes. Nowadays, the phenolic composition of the wine without added SO2 have not been clearly reported.

Green pruning of shoots to force new sprouting of buds, in fruit set and in pea size: vegetative, productive and maturation effects, in cv. Verdejo

The context of climate crisis leads to the acceleration of technological ripening of grapes, with unsuitable loss of acidity, so various vineyard management alternatives are being considered to delay the grape ripening. The delay of the vegetative cycle towards a period of milder temperatures affects ripening, but vine behavior can vary according to the area, conduction, watering, variety, etc. A work is proposed to know the response to the green pruning of shoots, executed in fruit set and in pea size, in cv. Verdejo.