Cultivation forms and viticulture models adapting to adverse “environmental” conditions

In Castilla-La Mancha as other winegrowing regions, vineyards suffer the effects of the global climate warming. Severe spring and summer droughts are increasingly frequent, which concur with the phenological stages most susceptible to water and temperature stress. Under these conditions, irrigation use is required in order to ensure the vineyard growing sustainability. However water resources are increasingly limited, for this reason is required to choose cultivars displaying high water use efficiency.

Il Nebbiolo, uno dei vitigni più rappresentativi della viticoltura piemontese. é caratterizzato da una maturazione tardiva, una elevata vigoria e una bassa fertilità basale. La sua popolazione inoltre presenta una tale variabilità morfologica che é consuetudine suddividere il vitigno in diverse sottovarietà (Lampia, Rosé, Michet, Balla per citare solo quelle dell’areale albese) ognuna con presunte distinte caratteristiche morfologiche e produttive.

When considering the application of a systemic approach to assess the intrinsic complexity of agricultural production, the following question immediately arises

The main objective is to unravel the yeast biosynthetic pathways for MEL, SER and HT by using the respective labelled amino acids precursors: 15N2-L tryptophan and 13C-tyrosine.
The alcoholic fermentation experiments are performed with two different commercial
S cereviseae yeasts using synthetic must with the addition of the labelled compounds and the bioactive compounds were followed during the fermentation process. Six biological replicates of the fermentations were considered. MEL, SER and HT were analysed by UHPLC coupled to High Resolution Mass Spectrometry (HRMS). Accurate mass determination allowed to unequivocally distinguishing labelled and unlabelled compounds.

The C-glycosidic ellagitannins constitute a subclass of hydrolyzable tannins of remarkable structural diversity. In this work we first achieved the hemisynthesis of flavano-ellagitannins, then we used them to develop a new efficient detection and quantification procedure for the C-glycosidic ellagitannins as well as flavano-ellagitannins.