Caractéristiques édaphiques et potentialités qualitatives des terroirs du vignoble languedocien

Obtaining high-quality RNA from grape tissues, including berry pulp, berry skins, stems, rachis, or roots, is challenging due to their composition, which includes polysaccharides, phenolic compounds, sugars, and organic acids that can negatively affect RNA extraction. For instance, polyphenols and other secondary metabolites can bind to RNA, making it difficult to extract a pure sample. Additionally, RNA can co-precipitate with polysaccharides, leading to lower extraction yield. Also, sugars and organic acids can interfere with the pH and ionic properties of the extraction buffer. To address these challenges, we optimized a protocol for RNA isolation from grape tissues.

The study of the interaction between vineyards and the environment to establish the grapevines in the appropriate places has been applied in wine science for 5000 years. Advances in the field of the zoning have not been uniform in time, and have occupied a preferential place in the contributions of Roman writers of the 1st Century AC, the contemplations of Tokay (1700) and Porto (1756) and works of the second half of the 20th century. Zoning practices today integrate multidisciplinary methodologies (viticulture, enology, soils, climatology, cartography, statistics, computer science) and require further development for future application.

The precise molecular mechanisms underlying the domestication of grapevine (Vitis vinifera L.) Are still not fully understood. In the recent years, next-generation sequencing (NGS) of plastid genomes has emerged as a powerful and increasingly effective tool for plant phylogenetics and evolution. To uncover the biological profile of the grapevine domestication process comprehensively, an investigation should encompass both the cultivated varieties (V. vinifera subsp. Vinifera) and their wild ancestors V. vinifera subsp. Sylvestris) across all potential sites of their distribution and domestication.

Yeast-derived aroma compounds are the result of different and complex biochemical pathways that mainly occur during alcoholic fermentation. Many of them are related -but not limited- to the availability of nutrients in the fermentation medium and linked to nitrogen metabolism and biomass produced. Besides, the metabolic phase of yeast also regulates the expression of many enzymes involved in the formation of aroma active compounds. The work investigates the overall effect of continuous supplementation of nutrients during alcoholic fermentation of a grape must on the volatile composition of wines.

Correlations between vineyard site and wine have, historically, been limited due to lack of uniformity in scion and rootstock clone and lack of controlled pilot-scale winemaking conditions, particularly temperature