Improvement of sparkling wines production by a zoning approach in Franciacorta (Lombardy, Italy)

Flavescence dorée (FD) poses a significant threat to grapevine health, with the American grapevine leafhopper, Scaphoideus titanus, serving as the primary vector.

Developments in high-throughput sequencing (HTS) technologies allow us to obtain large amounts of microbial information from wine and must samples. Thus approaches, that are aimed at characterising the microbial diversity during fermentation, can be enhanced, or possibly even replaced, with HTS-based metabarcoding. To reduce experimental biases and increase data reproducibility, we compared 3 DNA extraction methods by evaluating differences in the fungal diversity with Riesling alcoholic fermentation samples at four different vineyards. The fungal diversity profiling was done using the genetic markers ITS2 and D2 using metabarcoding. The extraction methods compared consisted of a commercial kit, a recently published protocol that includes a DNA enhancer, and a protocol based on a buffer containing common inhibitor removal reagents. All methods were able to distinguish vineyard effects on the fungal diversity, but the results differed quantitatively.

The poster presents the results of the 3rd year of activity of the project “Characterization of the wine productions of the italian regions. The DOC wine Colli Piacentini Gutturnio”.

In plants, stress due to nutrient deficiency can significantly impair their development and productivity.

Proximal sensors are becoming widely used in precision viticulture, due to the quick, easy and non-invasive identification of soil spatial variability. The apparent soil electrical conductivity (ECa) is the main parameter measured by sensors, which is correlated to many factors, like soil water content, salinity, clay content and mineralogy, rock fragments, bulk density, and porosity.