Chitosan elicits mono-glucosylated stilbene production and release in fed-batch fermentation of grape cells
Abstract
In the present study, the optimal conditions of grape (Vitis vinifera cv ‘Barbera’) cell cultures in batch and fed-batch bioreactor processes were studied to specifically improve the production of mono-glucosylated stilbenes. With respect to jars, bioreactor system induced higher polyphenol yields and largely increased their release in the culture medium. The highest stilbene production was reached in fed-batch bioreactor process supplied with 50 mg L-1 chitosan, that improved the accumulation of both free and mono-glucosylated resveratrol derivatives. Moreover, the vast majority of the compounds was released in the culture media, this being a considerable advantage for the bioreactor production and easier recovery of specific molecules or of polyphenol enriched mixtures as a promising alternative to whole plant extraction.
Issue: Macrowine 2010
Type: Article
Authors
Department of Experimental Evolutionary Biology, University of Bologna, Via Irnerio 42, 40126 Bologna I.