L’Appellation d’Origine Contrôlée « Huile Essentielle de Lavande de Haute Provence »

The Matrix Assisted Laser Desorption/Ionization–Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technology is commonly used in food and medical sector to identify yeast or bacteria species isolated from a nutritive culture media. Since a decade, brewery and oenology industries have been attracted to this method which combines fast analysis times, reliability and low cost of analysis. Briefly, this method is based on the comparison of the MALDI-TOF/MS protein spectra of an isolated colony of yeast or bacteria with those contain in a manufacturer’s reference protein spectra database. Initiated in 2015, the creation of the first oenological mass spectra database has proved to be essential for increase quality of species identification.

Aims: Viticulture practices linked with soil management, as cover crops and deficit irrigation, can help to regulate the vineyard behavior reducing in most cases plant vigor and modifying plant water and nutrient status, and as a consequence, grape yield and quality. Also, these practices can modify the soil biological activity mostly related to microbiome diversity and functionality.

Vineyard nutrient management is crucial for reaching production-specific quality standards, yet timely evaluation of nutrient status remains challenging. The existing sampling protocol of collecting vine tissue (leaves and/or petioles) at bloom or veraison is time-consuming. Additionally, this sampling practice is too late for in-season fertilizer applications (e.g. N is applied well before bloom). Therefore alternative early-season protocols are necessary to predict the vine nutrient demand for the upcoming season. The main goals of this project are to 1) optimize existing tissue sampling protocols; 2) determine the amount of nutrients removed at the end of the growing season.

A soil management and vine irrigation trial was carried out for 4 consecutive years from 2020 to 2023 at agroscope’s experimental vineyard in leytron (Valais, Switzerland) with the Chasselas grape variety (clone 14-33/4, grafted on 5bb). Two types of soil maintenance (bare soil with chemical weeding and sown grass) coupled with two water regimes (with and without drip irrigation from flowering to veraison) were compared in a randomized design with four replicates of 10 vines each.

During alcoholic fermentation, nitrogen is an essential nutrient for yeast as it plays a key role in sugar transport and biosynthesis of wine aromatic compounds (thiols, esters, higher alcohols). The main issue of a lack in yeast assimilable nitrogen (yan) in winemaking is sluggish or stuck fermentations promoting the growth of alteration species which may lead to economic losses. However, correcting this nitrogen deficiency is sometimes not enough to restore proper fermentation performance. This suggests the existence of other nutritional shortages.