WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 4 - WAC - Oral presentations 9 Wine lees: characterization and valorization by kombucha fermentation

Wine lees: characterization and valorization by kombucha fermentation

Abstract

Winemaking generates various types of residues (vine shoots, stalks, pomace, wine lees and filtration cakes) which can have a notable environmental and economic impact. Wine by-products are rich in bioactive compounds and therefore their valorization can be beneficial on different levels. Lees are the material that settles after vinification, and consist mainly of yeast cells, grape skins, tartrates, phenolic compounds, and other residues. The objective of this study was to valorize the wine lees by the Kombucha process in order to create a new beverage. 

Kombucha is a traditional beverage obtained by the fermentation of sweetened tea with a symbiotic culture of yeast and bacteria. The consumption of kombucha is associated with many health benefits due to its rich composition in bioactive compounds. Different substrates were used as raw material for Kombucha fermentation, and the obtained beverages displayed an increase in the concentration of biological compounds and enhancement of health activities. 

Red wine lees used in this study presented a pH of 3.31 ± 0.01, a total acidity of 2.86 ± 0.45 g/L (sulfuric acid equivalent), a total polyphenol content of 2041 ± 233.35 mg/L GAE (Gallic acid equivalent), and an antioxidant activity of 59.03 ± 4.25 % inhibition against DPPH radicals. 

In order to ferment the wines lees by Kombucha Scoby, wine lees were subject to two dilutions of 1:2 and 1:4. These dilutions were fermented for 24 days at 25°C. Samples were taken each 3 days in order to monitor the physico-chemical evolution of the new beverage. Results showed that the sugar consumption (70 g/L) was not complete after 24 days of fermentation. pH of the new beverage is 2.88. Fermentation time and substrate concentration influenced the studied variables, for instance the strongest antioxidant activity was detected on the 9th day for the lees kombucha diluted to the half (93.27%) whereas the highest quantity of polyphenols was found on day 21 (1599.30 mg/L GAE).

DOI:

Publication date: June 14, 2022

Issue: WAC 2022

Type: Article

Authors

Youssef El Rayess, Nathalie Barakat, Sandra Beaufort, Samar Azzi-Achkouty, Ziad Rizk, Chantal Ghanem, Abdo Tannoury, Jalloul Bouajila, Patricia Taillandier, Youssef El Rayess

Presenting author

Youssef El Rayess – Department of Agriculture and Food Engineering, Holy spirit University of Kaslik, Jounieh, Lebanon

Laboratoire de Génie Chimique, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France | Laboratoire de Génie Chimique, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France | Department of Agriculture and Food Engineering, School of Engineering, Holy Spirit University of Kaslik, Jounieh, Lebanon | Lebanese Agricultural Research Institute, Fanar Station, P.O. Box 90-1965, Jdeidet El-Metn, Fanar, Lebanon | Lebanese Agricultural Research Institute, Fanar Station, P.O. Box 90-1965, Jdeidet El-Metn, Fanar, Lebanon | Lebanese Agricultural Research Institute, Fanar Station, P.O. Box 90-1965, Jdeidet El-Metn, Fanar, Lebanon | Laboratoire de Génie Chimique, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France | Laboratoire de Génie Chimique, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France | Department of Agriculture and Food Engineering, School of Engineering, Holy Spirit University of Kaslik, Jounieh, Lebanon 

Contact the author

Keywords

wine lees-Kombucha-polyphenols-antioxidants

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Assessment of the impact of actions in the vineyard and its surrounding environment on biodiversity in Rioja Alavesa (Spain)

Traditional viticulture areas have experienced in the last decades an intensification of field practices, linked to an increased use of fertilisers and phytosanitary products, and to a more intensive mechanization and uniformization of the landscape. This change in management has sometimes led to higher rates of soil erosion andloss of soil structure, fertility decline, groundwater contamination, and to an increased pressure of pests and diseases. Additionally, intensification usually leads to a simplification of landscapes, of particular concern in prestigious wine grape regions where the economical revenue encourages the conversion of land use from natural habitats to high value wine grape production. To revert this trend, it is necessary that growers implement actions that promote biodiversity in their vineyards. The aim of this study is to assess the impact of the implementation of cover crops, vegetational corridors, dry stone walls and vineyard biodiversity hotspots estimated through the study of arthropods. The work has been carried out in four vineyards in Rioja Alavesa belonging to Ostatu winery, where these infrastructures were implemented in 2020. The presence and diversity of arthropods was studied by capturing them at different times in the season and at different distances from the infrastructure using pit-fall traps in the soil and yellow, white and blue chromatic traps at the canopy level. This is a preliminary study in which all adult insects were sorted to the taxonomic level of order and Coleoptera were classified to morphospecies. The results obtained show that there is a relationship between the basic characteristics of the vineyard and the arthropods captured, with a positive effect, although also dependent on the vineyard, of the presence of infrastructure.

A blueprint for managing vine physiological balance at different spatial and temporal scales in Champagne

In Champagne, the vine adaptation to different climatic and technical changes during these last 20 years can be seen through physiological balance disruptions. These disruptions emphasize the general grapevine decline. Since the 2000s, among other nitrogen stress indicators, the must nitrogen has been decreasing. The combination of restricted mineral fertilizers and herbicide use, the growing variability of spring rainfall, the increasing thermal stress as well as the soil type heterogeneity are only a few underlying factors that trigger loss of physiological balance in the vineyards. It is important to weigh and quantify the impact of these factors on the vine. In order to do so, the Comité Champagne uses two key-tools: networking and modelization. The use of quantitative and harmonized ecophysiological indicators is necessary, especially in large spatial scales such as the Champagne appellation. A working group with different professional structures of Champagne has been launched by the Comité Champagne in order to create a common ecophysiology protocol and thus monitor the vine physiology, yearly, around 100 plots, with various cultural practices and types of soil. The use of crop modelling to follow the vine physiological balance within different pedoclimatic conditions enables to understand the present balance but also predict the possible disruptions to come in future climatic scenarios. The physiological references created each year through the working group, benefit the calibration of the STICS model used in Champagne. In return, the model delivers ecophysiology indicators, on a daily scale and can be used on very different types of soils. This study will present the bottom-up method used to give accurate information on the impacts of soil, climate and cultural practices on vine physiology.

Protected Designation of Origin (D.P.O.) Valdepeñas: classification and map of soils

The objective of the work described here is the elaboration of a map of the different types of vineyard soils that to guide the famers in the choice of the most productive vine rootstocks and varieties. 90 vineyard soils profiles were analysed in the entire territory of the Origen Denominations of Valdepeñas. The sampling was carried out in 2018 (June to October) by making a sampling grid, followed by photointerpretation and control in the field. The studied soils can be grouped into 9 different soil types (according to FAO 2006 classification): Leptosols, Regosols, Fluvisols, Gleysols, Cambisols, Calcisols, Luvisols and Anthrosols. A map showing the soil distribution with different type of soils has been made with the ArcGIS program. Regarding to the choice of rootstock, Calcisoles are soils with a high active limestone content, so the rootstocks used in these soils must be resistant to this parameter; Luvisols are deep soils with high clay content, so they will support vigorous rootstocks. Because the cartographic units are composed of two or more subgroups, with are associated in variable proportions, 9 different soil associations have been established; Unit 1: Leptosols, Cambisols and Luvisols (80%, 15% and 5% respectively); Unit 2: Cambisols with Regosols and Luvisols (40%, 30% and 30% respectively); Unit 3: Cambisols and Gleysols with Regosols (40%, 40% and 20% respectively); Unit 4: Regosols with Cambisols, Leptosols and Calcisols (40%, 30%, 15% and 15% respectively); Unit 5: Cambisols, Leptosols, Calcisols and Regosols (25% each of them); Unit 6: Luvisols with Cambisol and Calcisols (80%, 10% and 10% respectively); Unit 7: Luvisols and Calcisols with Cambisols (40%, 40% and 20% respectively); Unit 8: Calcisols with, Cambisols and Luvisols (80%, 10% and 10% respectively); Unit 9: Anthrosols. These study allow to elaborate the first map of vineyard soils of this Protected Designation of Origin in Castilla-La Mancha.

Biodiversity in the vineyard agroecosystem: exploring systemic approaches

Biodiversity conservation and restoration are essential for guarantee the provision of ecosystem services associated to vineyard agroecosystem such as climate regulation trough carbon sequestration and control of pests and diseases. Most of published research dealing with the complexity of the vineyard agroecosystems emphasizes the necessity of innovative approaches, including the integration of information at different temporal and spatial scales and development of systemic analysis based on modelling. A biodiversity survey was conducted in the Franciacorta wine-growing area (Lombardy, Italy), one of the most important Italian wine-growing regions for sparkling wine production, considering a portion of the territory of 112 ha. The area was divided into several Environmental Units (EUs), defined as a whole vineyard or portion of vineyard homogenous in terms of four agronomic characteristics: planting year, planting density, cultivar, and training system. In each EU a set of compartments was identified and characterised by specific variables. The compartments are meteorology, morphology (altitude, slope, aspect, row orientation, and solar irradiance), ecological infrastructures and management. The landscape surrounding EU was also characterised in terms of land-use in a buffer zone of 500 m. For each component a specific methodology was identified and applied. Different statistical approaches were used to evaluate the method to integrate the information related to different compartments within the EU and related to the buffer zone. These approaches were also preliminarily evaluated for their ability to describe the contribution of biodiversity and landscape components to ecosystem services. This methodological exploration provides useful indication for the development of a fully systemic approach to structural and functional biodiversity in vineyard agroecosystems, contributing to promote a multifunctional perspective for the all wine-growing sector.

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares