WAC 2022 banner
IVES 9 IVES Conference Series 9 WAC 9 WAC 2022 9 4 - WAC - Oral presentations 9 Polyphenols in kombucha: Metabolomic analysis of biotransformations during fermentation

Polyphenols in kombucha: Metabolomic analysis of biotransformations during fermentation

Abstract

Kombucha is a non-alcoholic beverage made of sugared tea that is transformed by a symbiotic consortium of yeasts and bacteria. This beverage is increasingly produced at industrial scale, but its quality standards remain to be defined. Metabolomics analysis was carried out using FT-ICR-MS to understand the chemical transformations induced by the production phases and the type of tea on the non-volatile compounds of kombucha. Of the two production phases, the first phase of acidification in open vessel was the most impactful on molecular diversity, but tea type mainly influenced the global composition in polyphenol profiles. Black tea polyphenols were more impacted by microbial activity compared to green tea polyphenols. Independently from tea type, the first phase was also characterized by the release of gluconate from acetic acid bacteria metabolism. Gallate was also released and probably originated from the hydrolysis of ester bounds located in polymeric flavan-3-ols. The biotransformation of antioxidant polyphenols could positively impact their bioavailability for the consumer.

DOI:

Publication date: June 14, 2022

Issue: WAC 2022

Type: Article

Authors

Thierry, Tran, Rémy, Romanet, Chloé, Roullier-Gall, Antoine, Martin, Hervé, Alexandre,Cosette, Grandvalet, Tourdot-Maréchal, Philippe, Schmitt-Kopplin

Presenting author

Thierry, Tran – UMR PAM – Team VAlMiS

SATT Sayens, Chloé, Roullier-Gall | UMR PAM – Team VAlMiS, François, Verdier | Biomère | Biomère | Helmholtz Zentrum München | UMR PAM – Team VAlMiS | UMR PAM – Team VAlMiS | UMR PAM – Team VAlMiS 

Contact the author

Keywords

kombucha, metabolomics, polyphenols, bioavailability, fermentation

Tags

IVES Conference Series | WAC 2022

Citation

Related articles…

Evaluation of the site index model for viticultural zoning

Une variable composite, dénommée Indice de Site (SI), intégrant les propriétés physiques du sol et le mésoclimat, avait été proposée pour caractériser les terroirs dans le cadre d’une étude des vignobles de Cabernet Sauvignon de Hawke’s Bay en Nouvelle Zélande.

Non-invasive quantification of phenol content during red wine fermentations

Phenolic compounds are responsible for the most important red wine quality attributes. Anthocyanins and tannins play crucial roles in color and mouthfeel properties of red wines. Phenolic analysis in the winery is hindered by analytical constrains.

Analytical and Chemometric Investigation of Phenolic Content of South African Red Wines

Phenolic compounds have been the focus of a lot of research in recent years for their important contribution to sensory characteristics of wine, their beneficial health effects, as well as the possibility they offer of characterising wines. In this contribution, a method is developed that allows the direct injection of wine samples followed by liquid

Use of mathematical modelling and multivariate statistical process control during alcoholic fermentation of red wine

Cyberphysical systems can be seen in the wine industry in the form of precision oenology. Currently, limitations exist with established infrared chemometric models and first principle mathematical models in that they require a high degree of sample preparation, making it inappropriate for use in-line,

Grapevine xylem embolism resistance spectrum reveals which varieties have a lower mortality risk in a future dry climate

Wine growing regions have recently faced intense and frequent droughts that have led to substantial economical losses, and the maintenance of grapevine productivity under warmer and drier climate will rely notably on planting drought-resistant cultivars. Given that plant growth and yield depend on water transport efficiency and maintenance of photosynthesis, thus on the preservation of the vascular system integrity during drought, a better understanding of drought-related hydraulic traits that have a significant impact on physiological processes is urgently needed. We have worked towards this end by assessing vulnerability to xylem embolism in 30 grapevine commercial varieties encompassing red and white Vitis vinifera varieties, hybrid varieties characterized by a polygenic resistance for powdery and downy mildew, and commonly used rootstocks. These analyses further allowed a global assessment of wine regions with respect to their varietal diversity and resulting vulnerability to stem embolism. Hybrid cultivars displayed the highest vulnerability to embolism, while rootstocks showed the greatest resistance. Significant variability also arose among Vitis vinifera varieties, with Ψ12 and Ψ50 values ranging from -0.4 to -2.7 MPa and from -1.8 to -3.4 MPa, respectively. Cabernet franc, Chardonnay and Ugni blanc featured among the most vulnerable varieties while Pinot noir, Merlot and Cabernet Sauvignon ranked among the most resistant. In consequence, wine regions bearing a significant proportion of vulnerable varieties, such as Poitou-Charentes, France and Marlborough, New Zealand, turned out to be at greater risk under drought. These results highlight that grapevine varieties may not respond equally to warmer and drier conditions, outlining the importance to consider hydraulic traits associated with plant drought tolerance into breeding programmes and modeling simulations of grapevine yield maintenance under severe drought. They finally represent a step forward to advise the wine industry about which varieties and regions would have the lowest risk of drought-induced mortality under climate change.