IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Hanseniaspora in wine-making: their genetic modification and potential role in acid modulation

Hanseniaspora in wine-making: their genetic modification and potential role in acid modulation

Abstract

Hanseniaspora spp. are one of the most common yeast isolates in vineyards and wineries and play an important role in wine-making.  We explored the impact of an apiculate yeast Hanseniaspora occidentalis strain as a co-partner with Saccharomyces cerevisiae in a sequential-type mixed-culture fermentation of Muscaris grape must.  Like with other fermentation trials using Hanseniaspora strains, a significant increase in ethyl acetate was observed, but most intriguing, was the almost complete abolition of malic acid in the wine.  Compared to the pure S. cerevisiae inoculum there was also a marked increase in the concentrations of the other acetate esters. Modulation of some of the varietal elements like rose oxide was also observed.  Identifying and confirming the genes involved in malic acid utilization and aroma formation would require the development of gene modification tools: a feature not existing yet in H. occidentalis. We have recently developed gene-modifying tools in Hanseniaspora uvarum where we knocked-out the two alleles of the alcohol acetyltransferase gene (HuATF) resulting in a yeast with much lower acetate ester capabilities. This was the first successful attempt to genetically remove a gene from Hanseniaspora and paves the way for further gene-to-function studies in this apiculate yeast
genus

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Van Wyk Niel¹, Badura Jennifer¹, Scansani Stefano¹,³, Pretorius Isak S.², Rauhut Doris1, Von Wallbrunn Christian¹

¹Department of Microbiology and Biochemistry, Hochschule Geisenheim University, Geisenheim, Germany
²ARC Centre of Excellence in Synthetic Biology, Department of Molecular Sciences, Macquarie University, Sydney, Australia
³Institute of Applied Microbiology, Justus-Liebig-University, Giessen, Germany

Contact the author

Keywords

Hanseniaspora, GMO, malic acid

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Ten years soil diagnosis in vineyards, with particularly analysis of organic and microbial mass and measuring their evolution

Since 1996, we study the soil in viticulture, specially in the South of France. In the field, we delimit soil units and observe soil profiles and take samples to analyse its physical, mineral, organic and microbial mass composition

Validation of a high-throughput method for the quantification of volatile carbonyl compounds in wine and its use in accelerated ageing experiments

the aim of this study was the optimization and validation of a robust and comprehensive method for the determination of volatile carbonyl compounds (VCCs) in wines

Moderated consumption of alcoholic beverages and cancer risk

One on three cases of cancer is associated with lifestyle and nutritional patterns, and the excessive intake of alcoholic beverages is a well established risk factor. Moderate drinking has been associated with reduced or increased risk of various types of cancer, but the clinical relevance of the risk rates has not been evaluated in ad hoc prospective investigations.

The influence of the soil on the phenolic composition of both grapes and wines : “the Grenache observatory”

La composition fine des raisins de Grenache noir est mal connue. Il est généralement admis une certaine variabilité de comportement de ce cépage qui se manifeste principalement sur la couleur des vins. De nombreux facteurs peuvent être à l’origine de cette variabilité : matériel végétal, pratiques culturales, types de vinification et terroir. Un travail de recherche concernant ce cépage a été engagé dans la Vallée du Rhône.

Modeling island and coastal vineyards potential in the context of climate change

Climate change impacts regional and local climates, which in turn affects the world’s wine regions. In the short term, these modifications rises issues about maintaining quality and style of wine, and in a longer term about the suitability of grape varieties and the sustainability of traditional wine regions. Thus, adaptation to climate change represents a major challenge for viticulture. In this context, island and coastal vineyards could become coveted areas due to their specific climatic conditions. In regions subject to warming, the proximity of the sea can moderate extremes temperatures, which could be an advantage for wine. However, coastal and island areas are particular prized spaces and subject to multiple pressures that make the establishment or extension of viticulture complex.
In this perspective, it seems relevant to assess the potentialities of coastal and island areas for viticulture. This contribution will present a spatial optimization model that tends to characterize most suitable agroclimatic patterns in historical or emerging vineyards according to different scenarios. Thanks to an in-depth bibliography a global inventory of coastal and insular vineyards on a worldwide scale has been realized. Relevant criteria have been identified to describe the specificities of these vineyards. They are used as input data in the optimization process, which will optimize some objectives and spatial aspects. According to a predefined scenario, the objectives are set in three main categories associated with climatic characteristics, vineyards characteristics and management strategies. At the end of this optimization process, a series of maps presents the different spatial configurations that maximize the scenario objectives.