IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Hanseniaspora in wine-making: their genetic modification and potential role in acid modulation

Hanseniaspora in wine-making: their genetic modification and potential role in acid modulation

Abstract

Hanseniaspora spp. are one of the most common yeast isolates in vineyards and wineries and play an important role in wine-making.  We explored the impact of an apiculate yeast Hanseniaspora occidentalis strain as a co-partner with Saccharomyces cerevisiae in a sequential-type mixed-culture fermentation of Muscaris grape must.  Like with other fermentation trials using Hanseniaspora strains, a significant increase in ethyl acetate was observed, but most intriguing, was the almost complete abolition of malic acid in the wine.  Compared to the pure S. cerevisiae inoculum there was also a marked increase in the concentrations of the other acetate esters. Modulation of some of the varietal elements like rose oxide was also observed.  Identifying and confirming the genes involved in malic acid utilization and aroma formation would require the development of gene modification tools: a feature not existing yet in H. occidentalis. We have recently developed gene-modifying tools in Hanseniaspora uvarum where we knocked-out the two alleles of the alcohol acetyltransferase gene (HuATF) resulting in a yeast with much lower acetate ester capabilities. This was the first successful attempt to genetically remove a gene from Hanseniaspora and paves the way for further gene-to-function studies in this apiculate yeast
genus

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Van Wyk Niel¹, Badura Jennifer¹, Scansani Stefano¹,³, Pretorius Isak S.², Rauhut Doris1, Von Wallbrunn Christian¹

¹Department of Microbiology and Biochemistry, Hochschule Geisenheim University, Geisenheim, Germany
²ARC Centre of Excellence in Synthetic Biology, Department of Molecular Sciences, Macquarie University, Sydney, Australia
³Institute of Applied Microbiology, Justus-Liebig-University, Giessen, Germany

Contact the author

Keywords

Hanseniaspora, GMO, malic acid

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Evaluation of the sensory profile of doc douro red wines through sensory traditional single-point techniques and temporal dominance methods

No other agricultural product has a stronger relationship with the soil than wine. This study aimed to characterize the sensory profile of red wines from the Douro Demarcated Region (RDD) certified as DOC Douro, through the application of Quantitative Descriptive Analysis (QDA®) and Temporal Dominance of Sensations (TDS) sensory methods. QDA® provides a complete word description for all a product’s sensory properties. The TDS, which is relatively recent in the sensory field [1], allows to evaluation and description of the evolution of the dominant sensory perceptions during the tasting of a food product.Eighteen commercial wines from different producers were evaluated, six different samples representing each of the three sub-regions of the RDD.

NEW TOOL FOR SIMULTANEOUS MEASUREMENT OF OXYGEN CONSUMPTION AND COLOUR MODIFICATIONS IN WINES

Measuring the effect of oxygen consumption on the colour of wines as the level of dissolved oxygen decreases over time is very useful to know how much oxygen a wine is able to consume without significantly altering its colour. The changes produced in wine after being exposed to high oxygen concen-trations have been studied by different authors, but in all cases the wine has been analysed once the oxygen consumption process has been completed. This work presents the results obtained with the use of an equipment designed and made to measure simultaneously the level of dissolved oxygen and the spectrum of the wine, during the oxygen consumption process from saturation levels with air to very low levels, which indicate the total consumption of the dosed oxygen.

Zonazione e vitigni autoctoni nel sud della Basilicata: metodologie integrate per la caratterizzazione di ambienti di elezione di biotipi storici finalizzati a vini di territorio nella DOC “Terre dell’Alta Val d’Agri”

I territori della DOC “Terre dell’Alta Val d’Agri”, a Sud della regione Basilicata, si caratterizzano per una elevata biodiversità autoctona autoselezionatesi su ambienti ecologicamente ben definiti,

Assessment of plant water consumption rates under climate change conditions through an automated modular platform

The impact of climate change is noticeable in the present weather, making water scarcity the most immediate mediator reducing the performance and viability of crops, including grapevine (Vitis vinifera L.). The present study developed a system (hardware, firmware, and software) for the determination of plant water use through changes in weight through a period. The aim is to measure the differences in grapevine water consumption in response to climate change (+4oC and 700 ppm) under controlled conditions. The results reveal a correlation between daily plant consumption rates and reference evapotranspiration (ETo).

UNCOVERING THE ROLE OF BERRY MATURITY STAGE AND GRAPE GENOTYPE ON WINE CHARACTERISTICS: INSIGHTS FROM CHEMICAL CHARACTERISTICS AND VOLATILE COMPOUNDS ANALYSIS

In a climate change context and aiming for sustainable, high-quality Bordeaux wine production, this project examines the impact of grape maturity levels in various cultivars chosen for their adaptability, genetic diversity, and potential to enhance wine quality. The study explores the effects on wine compo-sition and quality through sensory and molecular methods. We studied eight 14-year-old Vitis vinifera cv. grape varieties from the same area (VITADAPT plots 1 and 5): Cabernet Franc, Cabernet Sauvignon, Carmenère, Castets, Cot, Merlot, Petit Verdot, and Touriga Nacional.