IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Unravelling the microbial community structure and aroma profile of Agiorgitiko wine under different inoculation schemes

Unravelling the microbial community structure and aroma profile of Agiorgitiko wine under different inoculation schemes

Abstract

Agiorgitiko (Vitis vinifera L. cv.) is the most widely cultivated indigenous red grape variety in Greece, known for the production of Protected Designation of Origin Nemea wines. The aim of the present study was to evaluate five different combinations of yeast starters, previously isolated from spontaneous alcoholic fermentation of the same grape variety, for their oenological potential in terms of fermentation predominance and capacity as well as aromatic contribution to Agiorgitiko wine production. Grapes from the Nemea region, crashed and pressed, were inoculated with different yeast species/strains in pure and mixed cultures.  In particular, wines were produced in duplicate with the addition of (A) Saccharomyces cerevisiae SFA1, (B) S. cerevisiae SFA2, (C) S. cerevisiae SFA3, (D) S. cerevisiae SFA3, Hanseniaspora opuntiae SFB1 and (E) S. cerevisiae SFA3, H. opuntiae SFB1, H. opuntiae SFB2 and Hanseniaspora uvarum SFC1. At specific time points during the alcoholic fermentation, amplicon-based metagenomics analysis was employed to unravel the microbial community structure at the genus level. In the end of the fermentation process oenological parameters including volatile acidity, residual sugars and ethanol were determined according to the OIV protocols while the volatile compounds produced were measured by GC/MS. Finally, all produced wines were evaluated  by quantitative descriptive analysis. As expected, Saccharomyces dominated the yeast/fungal microbiota of the A-C wine samples throughout fermentation, followed by Aspergillus, Cladosporium and Aureobasidium, mainly at the early fermentation stage. In D and E wine samples, although Hanseniaspora was the predominant genus in early fermentation, the relative abundance of Saccharomyces rapidly increased and dominated until the end of the fermentation. Compared to yeast/fungi, bacterial community was characterized by a quite higher diversity. Although similar genera were identified in all wine samples (A-E), e.g. Bacillus, Oenococcus, Lactococcus, Staphylococcus and Acinetobacter, their relative abundances varied depending on the sample and fermentation stage. As far as the volatile profile was concerned, the GC/MS analysis revealed that the use of different species/yeasts modified the flavor and aroma of the produced wines. More specifically, exceptional amounts of higher alcohols and medium-chain fatty acid esters (known for their floral and fruity contribution) were observed in the co-inoculated wines (D and E), resulting in a more distinct and intense aromas. According to sensory evaluation the co-inoculation with three different yeast species (wine sample E) significantly increased the aromatic typicity characterized by red fruits aromas. Understating the microbial community structure during the alcoholic fermentation could lead to higher quality wine product and constitute a strong tool to direct wine sensory traits

Acknowledgments

This research has been co-financed by the European Union and Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation, under the call “Greece – Israel Call for Proposals for Joint R&D Projects 2019” (project code: T10ΔΙΣ-00060).

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Dimopoulou Maria¹, Kazou Maria², Drosou Fotini¹, Sellas Vassilis¹, Dourtoglu Vassilis¹ and Tsakalidou Effi²

¹Department of Wine, Vine, and Beverage Sciences, School of Food Science, University of West Attica, Athens, Greece
²Laboratory of Dairy Research, Department of Food Science and Human Nutrition, Agricultural University of Athens, Athens, Greece

Contact the author

Keywords

amplicon-based metagenomics analysis, wine aromas, regional yeast, Agiorgitiko

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.

Modeling island and coastal vineyards potential in the context of climate change

Climate change impacts regional and local climates, which in turn affects the world’s wine regions. In the short term, these modifications rises issues about maintaining quality and style of wine, and in a longer term about the suitability of grape varieties and the sustainability of traditional wine regions. Thus, adaptation to climate change represents a major challenge for viticulture. In this context, island and coastal vineyards could become coveted areas due to their specific climatic conditions. In regions subject to warming, the proximity of the sea can moderate extremes temperatures, which could be an advantage for wine. However, coastal and island areas are particular prized spaces and subject to multiple pressures that make the establishment or extension of viticulture complex.
In this perspective, it seems relevant to assess the potentialities of coastal and island areas for viticulture. This contribution will present a spatial optimization model that tends to characterize most suitable agroclimatic patterns in historical or emerging vineyards according to different scenarios. Thanks to an in-depth bibliography a global inventory of coastal and insular vineyards on a worldwide scale has been realized. Relevant criteria have been identified to describe the specificities of these vineyards. They are used as input data in the optimization process, which will optimize some objectives and spatial aspects. According to a predefined scenario, the objectives are set in three main categories associated with climatic characteristics, vineyards characteristics and management strategies. At the end of this optimization process, a series of maps presents the different spatial configurations that maximize the scenario objectives.

Climate projections over France wine-growing region and its potential impact on phenology

Climate change represents a major challenge for the French wine industry. Climatic conditions in French vineyards have already changed and will continue to evolve. One of the notable effects on grapevine is the advancing growing season. The aim of this study is to characterise the evolution of agroclimatic indicators (Huglin index, number of hot days, mean temperature, cumulative rainfall and number of rainy days during the growing season) at French wine-growing regions scale between 1980 and 2019 using gridded data (8 km resolution, SAFRAN) and for the middle of the 21th century (2046-2065) with 21 GCMs statistically debiased and downscaled at 8 km. A set of three phenological models were used to simulate the budburst (BRIN, Smoothed-Utah), flowering, veraison and theoretical maturity (GFV and GSR) stages for two grape varieties (Chardonnay and Cabernet-Sauvignon) over the whole period studied. All the French wine-growing regions show an increase in both temperatures during the growing season and Huglin index. This increase is accompanied by an advance in the simulated flowering (+3 to +9 days), veraison (+6 to +13 days) and theoretical maturity (+6 to +16 days) stages, which are more noticeable in the north-eastern part of France. The climate projections unanimously show, for all the GCMs considered, a clear increase in the Huglin index (+662 to 771 °C.days compared to the 1980-1999 period) and in the number of hot days (+5.6 to 22.6 days) in all the wine regions studied. Regarding rainfall, the expected evolution remains very uncertain due to the heterogeneity of the climates simulated by the 21 models. Only 4 regions out of 21 have a significant decrease in the number of rainy days during the growing season. The two budburst models show a strong divergence in the evolution of this stage with an average difference of 18 days between the two models on all grapevine regions. The theoretical maturity is the most impacted stage with a potential advance between 40 and 23 days according to wine-growing regions.

Late frost protection in Champagne

Probably one of the most counterintuitive impacts of climate change on vine is the increased frequency of late frost. Champagne, due to its septentrional position is historically and regularly affected by this meteorological hazard. Champagne has therefore developed a strong experience in frost protection with first experiments dating from the end of 19th century. Frost protection can be divided in two parts: passive and active. Passive protection includes all the methods that do not seek to modify the vine’s environment or resistance at the time of frost. The most iconic passive protection in Champagne is the establishment of the individual reserve. This reserve allows to stock a certain quantity of clear wine during a surplus year to compensate a meteorological hazard like frost during the following years. Other common passive methods are the control of planting area (walls, bushes, topography), the choice of grape variety, late pruning, or the impact of grass cover and tillage. Active frost protection is also divided in two parts. Most of the existing techniques tend to modify vine’s environment. Most of the time they provide warmth (candles, heaters, windmills, heating cables…), or stabilise bud’s temperature above a lethal threshold (water sprinkling). The other way to actively fight is to enhance the resistance of buds to frost (elicitors). The Comité Champagne evaluates frost protection methods following three main axes: the efficiency, the profitability, and the environmental impact through a lifecycle assessment. This study will present the results on both passive and active protection following these three axes.

Inhibition of Oenococcus oeni during alcoholic fermentation by a selected Lactiplantibacillus plantarum strain

The use of selected cultures of the species Lactiplantibacillus plantarum in Oenology has grown in prominence in recent years. While initial applications of this species centred very much around malolactic fermentation (MLF), there is strong evidence to show that certain strains can be harnessed for their bio-protective effects. Unwanted spontaneous MLF during alcoholic fermentation (AF), driven by rogue Oenococcus oeni, is a winemaking deviation that is very difficult to manage when it occurs. This work set out to determine the efficacy of one particular strain of Lactiplantibacillus plantarum(Viniflora® NoVA™ Protect), against this problem in Cabernet Sauvignon must. The work was carried out at commercial scale and in a winery environment and compared the bio-protective culture with the more traditional approach of reducing must pH by the addition of tartaric acid. The combination of both was also investigated. The concentration of both Oenococcus oeni and Lactiplantibacillus plantarum was determined using qPCR. The adventitious Oenococcus oeni showed the most growth during AF in the control wine, whereas in the wines treated with Lactiplantibacillus plantarum a bacteriostatic effect against this species was observed. This effect was comparable to the wines treated with tartaric acid. This has particular commercial relevance for controlling the flora in musts with high pH, or when the addition of tartaric acid is either not permitted or is prohibitive for other reasons.