IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Exploring and unravelling the complex toasted oak wood (Q. sp.) volatilome using GCxGC-TOFMS technique

Exploring and unravelling the complex toasted oak wood (Q. sp.) volatilome using GCxGC-TOFMS technique

Abstract

For coopers, toasting process is considered as a crucial step in barrel production where oak wood develops several specific aromatic nuances released to the wine during its maturation. Toasting is applying varying degrees of heat to a barrel over a specific amount of time. Today it is well known that as the temperature increases, thermal degradation of oak wood structure produces a huge range of chemical compounds. Indeed, many works were conducted to identify key aroma volatile compounds (e.g., whisky-lactone, furfural, maltol, eugenol, guaiacol, vanillin) using the traditional gas chromatography coupled with olfactometry and mass spectrometry (GC-O-MS).

Inspired by recent untargeted approaches in food “omics”, this work aims at expanding our knowledge on oak wood volatile composition by bi-dimensional comprehensive gas chromatography-time of flight mass spectrometry (GCxGC-TOFMS, BT4D, Leco).

In a first experiment, five toasting levels were selected and applied to Q. sessilis oak wood samples (control, 160 °C, 180 °C, 200 °C and 220 °C, 30 min, n=3). Organic extracts were prepared (dichloromethane, 50 g/L) and analysed by GCxGC-TOFMS on conventional column combination nonpolar/midpolar (DB-5ms/Rxi-17Sil). The separation was followed by a non-targeted approach for data processing. The resulting mass spectra (TIC) were de convoluted (ChromaTOF software) and compared to spectra from a database for tentative peak identification. It was necessary to restrict the number of processed peaks by applying some “filters” such as signal to noise (S/N > 50), linear retention index (LRI ± 30), mass spectra similarity (> 750) and repeatability level. Supervised multivariate and univariate statistical approaches were used to identify potential markers of toasting intensity. Thanks to R script, reproducible peaks number was reduced from about 15000 to 568. By comparing observed retention indices with those found in the literature, 77 of the identifications have been confirmed and associated with an increase in toasting intensity. Some of them were sensory active and well known in oak wood, such as guaiacol, creosol and isoeugenol. Others were identified for the first time in toasted oak wood such as 2-methylbenzofurane (burnt) and 2-hydroxy-2-cyclopenten-1-one (caramel).Additional results were also discussed on the capability of GCxGC-TOFMS to identify oak wood botanic origins (Q. robur, Q. alba

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Courregelongue Marie1,², Albertin Warren1,³, Prida Andrei2 and Pons Alexandre1,²

¹UMR ŒNOLOGIE (OENO), ISVV, UMR 1366, Université de Bordeaux, INRAE, Bordeaux INP
²Tonnellerie Seguin Moreau, Merpins, France
³ENSCBP, Bordeaux INP, 33600, Pessac, France

Contact the author

Keywords

non-targeted analysis, GCxGC-TOFMS, oak wood, toasting process, volatile compounds

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

CONSENSUS AND SENSORY DOMINANCE ARE DEPENDENT ON QUALITY CONCEPT DEFINITIONS

The definition of the term “quality” in sensory evaluation of food products does not seem to be consensual. Descriptive or liking methods are generally used to differentiate between wines (Lawless et al., 1997). Nevertheless, quality evaluation of a product such as wine can also relate to emotional aspects. As exposed by Costell (2002), product quality is defined as an integrated impression, like acceptability, pleasure, or emotional experiences during tasting. According to the ‘modality appropriateness’ hypothesis which predicts that wine tasters weigh the most suitable sensory inputs for a specific assess- ment (Freides, 1974; Welch & Warren, 1980), the nature of the quality definitions may modulate sensory influences.

Evoluzione stagionale delle temperature ed andamento della maturazione nel vitigno Aglianico: risultati di un quadriennio di osservazioni in Campania

In viticoltura, la comprensione dell’influenza della temperatura dell’aria sulla dinamica della maturazione assume importante rilievo in relazione all’ ottimizzazione dell’ epoca di raccolta da cui dipende in modo significativo la qualità del prodotto finale.

Identification of aroma markers in amarone wines

Amarone is an Italian red wine produced in the Valpolicella area, in north-eastern Italy. Due to its elaboration with withered grapes, Amarone is a rather unique example of dry red wine. However, there is very limited data so far concerning the volatile composition of commercial Amarone wines, which also undergo a cask aging of 2-4 years before release. The present work aims at characterizing the aroma composition of Amarone and to elucidate the relationships between chemical composition and sensory characters. The analysis of 17 Amarone commercial wines from the same vintage (2015) was carried out by means of Gas Chromatography-Mass Spectrometry (GC-MS) and extracted by Solid Phase Extraction (SPE) and Solid Phase Micro Extraction (SPME). In addition, the sampled wines were subjected to a sensory evaluation in the form of sorting task.RESULTS: 70 volatile compounds were successfully identified and quantified, 30 of which were present in concentrations above their odor thresholds in all the samples. Using the odor activity value (OAV), the compounds that potentially contribute to Amarone perceived aroma are b-damascenone, ethyl and isoamyl acetate, ethyl esters (hexanoate, octanoate, butanoate, 3-methybutanoate), 4-ethyl guaiacol, 3-methylbutanoic acid, dimethyl sulfide (DMS), eugenol, massoia lactone, 1,4-cineol, TDN, cis/trans-whisky lactone. In certain samples, high OAVs were also observed for 4-ethyl phenol and 1,8-cineole.Results from the sorting task sensory analysis showed three clusters formed.

Proposal of zonification and characterization of terroirs in the Yalde-Najerilla-Uruñuela vine growing area (DOC Rioja, Spain), based on the soil influence

Natural Terroir Units (NTU) are being delimited in vine growing area DOCa Rioja, in collaboration with Uruñuela Cooperative, to characterized specific and singular Tempranillo (Vitis vinifera

Understanding wine as a sensory, emotional, and cognitive experience to promote and communicate conscious consumption

In the complex scenario that the wine industry and its promotion are currently facing, this research proposes a theoretical expansion of the traditional model used to understand the wine experience, namely the classic sensory, emotional and cognitive triad, moving toward a multidimensional approach that also incorporates cultural, symbolic and contextual dimensions in order to comprehend the conscious experience.