IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 NADES extraction of anthocyanins derivatives from grape pomace

NADES extraction of anthocyanins derivatives from grape pomace

Abstract

Grape pomace is one of the main by-products generated after pressing in wine-making. It’s valorization through the extraction of bioactive compounds is the answer for the development of sustainable processes. Nevertheless, in the recovery of anthocyanins derivatives, the extraction stage continues to be a limiting step. The nature of the sample and the type of solvent determine the efficiency of the process. Anthocyanins are responsible for the color of grapes and wine, a characteristic that is defined by their chemical structure. The color stability in their native form is affected by factors such as pH, temperature, and their chemical structure, which could limit their use as a natural colorant in the food industry. However, there are anthocyanin-derived pigments found in wines. The main interest in these pigments is that they present greater color stability against pH changes and SO2 bleaching than native anthocyanin. Emerging methods such as ultrasound-assisted eutectic mixture extraction have a high potential due to the low toxicity, chemical inertness with water, easy preparation, and huge biodegradability. There are four types of eutectic solvents, however, the mixtures that are formed from salt and a natural component such as alcohols, sugars, and organic acids are the most used in the extraction processes of bioactive compounds. The compounds were mixed with a 1:1 molar ratio with choline chloride (ChCl) as hydrogen bond acceptor (HBA) and its corresponding hydrogen bond donor (HBD) (malic acid, citric acid, tartaric acid, glucose and glycerol: urea), heated at a constant temperature of 80 °C and stirring at 700 rpm until a transparent liquid was obtained. Then the volume of distilled water corresponding to each mixture (30 % v / v) was added. The extraction was carried out in an ultrasonic bath at room temperature for 45 min. A solid-liquid ratio of 1 g of pomace per 10 ml of solvent was used. Total anthocyanins were determined using the differential pH method measured by a spectrophotometer. The highest extraction using NADES corresponds to the mixtures composed of choline chloride: glucose and choline chloride: urea: glycerol reaching a value of 4.57 and 4.36 mg eq of malvidin-3-O-glucoside /g of grape pomace respectively. On the other hand, no significant differences were found for a value of p≤0.05 between the mixtures containing citric acid and tartaric acid, obtaining values of 0.81 and 0.69 mg eq of malvidin -3-O-glucoside /g grape pomace respectively. Although it is known that anthocyanins are more stable in acidic media, the extraction values obtained for mixtures with acids were lower than expected

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Salas Erika¹, Castellanos-Gallo Lilisbet¹, Ballinas-Casarrubias Lourdes1, Espinoza-Hicks José Carlos¹and Hernández-Ochoa León¹

¹Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua

Contact the author

Keywords

grape pomace, extraction, anthocyanins derivatives, natural deep eutectic solvent (NADES)

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares

Genotypic variability in root architectural traits and putative implications for water uptake in grafted grapevine

Root system architecture (RSA) is important for soil exploration and edaphic resources acquisition by the plant, and thus contributes largely to its productivity and adaptation to environmental stresses, particularly soil water deficit. In grafted grapevine, while the degree of drought tolerance induced by the rootstock has been well documented in the vineyard, information about the underlying physiological processes, particularly at the root level, is scarce, due to the inherent difficulties in observing large root systems in situ. The objectives of this study were to determine genetic differences in the root architectural traits and their relationships to water uptake in two Vitis rootstocks genotypes (RGM, 140Ru) differing in their adaptation to drought. Young rootstocks grafted upon the Riesling variety were transplanted into cylindrical tubes and in 2D rhizotrons under two conditions, well watered and moderate water stress. Root traits were analyzed by digital imaging and the amount of transpired water was measured gravimetrically twice a week. Root phenotyping after 30 days reveal substantial variation in RSA traits between genotypes despite similar total root mass; the drought-tolerant 140Ru showed higher root length density in the deep layer, while the drought-sensitive RGM was characterised by shallow-angled root system development with more basal roots and a larger proportion of fine roots in the upper half of the tube. Water deficit affected canopy size and shoot mass to a greater extent than root development and architectural-related traits for both 140Ru and RGM, suggesting vertical distribution of roots was controlled by genotype rather than plasticity to soil water regime. The deeper root system of 140Ru as compared to RGM correlated with greater daily water uptake and sustained stomata opening under water-limited conditions but had little effect on above-ground growth. Our results highlight that grapevine rootstocks have constitutively distinct RSA phenotypes and that, in the context of climate change, those that develop an extensive root network at depth may provide a desirable advantage to the plant in coping with reduced water resources.

Rapid damage assessment and grapevine recovery after fire

There is increasing scientific consensus that climate changeis the underlying cause of the prolonged dry and hot conditions that have increased the risk of extreme fire weather in many countries around the world. In December 2019, a bushfire event occurred in the Adelaide Hills, South Australia where 25,000 hectares were burnt and in vineyards and surrounding areas various degrees of scorching and infrastructure damage occurred. The ability to coordinate and plan recovery after a fire event relies on robust and timely data. The current practice for measuring the scale and distribution of fire damage is to walk or drive the vineyard and score individual vines based on visual observation. The process is time consuming, subjective, or semi-quantitative at best. After the December 2019 fires, it took many months to access properties and estimate the area of vineyard damaged. This study compares the rapid assessment and mapping of fire damage using high-resolution satellite imagery with more traditional ground based measures. Satellite imagery tracking vineyard recovery in the season following the bushfire is being correlated to field assessments of vineyard productivity such as canopy health and development, fertility and carbohydrate storage. Canopy health in the seasons following the fires correlated to the severity of the initial fire damage. Severely damaged vines had reduced canopy growth, were infertile or had very low fertility as well as lower carbohydrate levels in buds and canes during dormancy, which reduced productivity in the seasons following the bushfire event. In contrast, vines that received minor damage were able to recover within 1-2 years. Tools that rapidly and affordably capture the extent and severity of damage over large vineyard area will allow producers, government and industry bodies to manage decisions in relation to fire recovery planning, coordination and delivery, improving the efficiency and effectiveness of their response.

Effects of graft quality on growth and grapevine-water relations

Climate change is challenging viticulture worldwide compromising its sustainability due to warmer temperatures and the increased frequency of extreme events. Grafting Vitis vinifera L.

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard