IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Impact of dosage sugar-type and ageing on finished sparkling wine composition and development of Maillard reaction-associated compounds

Impact of dosage sugar-type and ageing on finished sparkling wine composition and development of Maillard reaction-associated compounds

Abstract

The Maillard reaction (MR) is a non-enzymatic reaction between reducing sugars and amino acids, resulting in the production of volatile and flavour-active compounds. Existing MR research primarily addresses thermally processed foods, whereas limited studies have evaluated low-temperature MR environments including sparkling wine. Sparkling wine is produced in low temperature conditions (15 ± 3°C), with low pH (pH 3-3.4) and high acidity (titratable acidity 7-12 g/L) 1. Various MR species including furans, acryl amides, and heterocyclic amines have been identified in aged sparkling wines and contribute to roasted, caramel, and nutty aromas 2–4. The aim of this research was to investigate the composition of finished sparkling wine during 18-months of ageing by measuring the formation of target MR-associated compounds and the relative levels of precursor species (amino acids, reducing sugars). Variable liqueur de dosage sugar-types were assessed for their impact on MR-associated compounds during ageing. Liqueur de dosage (composed of sugar, wine, and SO2) is an addition made post-disgorgement, and these sugars may degrade or interact with amino acids, thereby influencing the formation of MR compounds. To the best of our knowledge, no prior literature has investigated the role of Liqueur de dosage in the MR. In this research, six dosage sugar treatments were evaluated including D-glucose, D-fructose, sucrose (cane-derived), sucrose (beet-derived), maltose, and commercial rectified grape must concentrate (RCGM), in addition to a zero-dosage/control (no sugar added). Treatments were carried out on 2015 vintage sparkling wine (3 years on lees; 59% Chardonnay, 41% Pinot Noir) produced by Niagara College Teaching Winery in Niagara-on-the-Lake, ON. Dosage treatments were prepared from the sparkling wine base to approximately 6 g/L residual sugar. Bottles were sealed with cork closures and cellared on-site at the Cool Climate Oenology & Viticulture Institute with environmental controls for temperature and humidity. At intervals of 0, 9 and 18-months post-dosage addition, triplicate bottles of each wine were chemically analyzed. MR-associated products were quantified by HS-SPME-GC-MS. Precursors including sugars and amino acids were quantified by enzymatic assay and NMR techniques, respectively, and sugar purity was determined by HPLC. After 18 months of aging post-disgorging, four MRPs showed concentration differences (p < 0.05) between dosage sugar treatments (ethyl 3-mercaptopropionate, furfuryl ethyl ether, 2-ethylthiazole, and 2-furyl methyl ketone). Changes in sugar and amino acid content during ageing were used to relate changes in MR compound formation with precursor consumption. This study establishes the effect of dosage sugar-type on the formation of volatile MR compounds in traditional method sparkling wines during ageing.

References

1. Kemp, B.; Alexandre, H.; Robillard, B.; Marchal, R. J. Agric. Food Chem. 2015, 63 (1), 19–38. 
2. Le Menn, N.; Marchand, S.; De Revel, G.; Demarville, D.; Laborde, D.; Marchal, R. J. Agric. Food Chem. 2017, 65 (11), 2345–2356. 
3. Keim, H.; De Revel, G.; Marchand, S.; Bertrand, A. J. Agric. Food Chem. 2002, 50 (21), 5803–5807. 
4. Marchand, S.; Almy, J.; de Revel, G. J. Food Sci. 2011, 76 (6), 861-868.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Charnock Hannah1, Pickering Gary J.1,2,3,4, Kemp Belinda S.1,2

1Department of Biological Sciences, Faculty of Mathematics & Science, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON, Canada, L2S 3A1
2Cool Climate Oenology & Viticulture Institute, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON, Canada, L2S 3A1
3National Wine and Grape Industry Center, Charles Sturt University, McKeown Drive, Wagga Wagga, NSW 2678, Australia
4Sustainability Research Centre, University of the Sunshine Coast, 90 Sippy Downs Drive, Sippy Downs, QLD 4556, Australia

Contact the author

Keywords

sparkling wine, Maillard reaction, time-course ageing

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Spatial determination of areas in the Western Balkans region favorable for organic production

In problematic conditions for production of grapes and wine caused by the COVID-19 pandemic and the resulting occurrence of wine surpluses, producers are increasingly turning to the innovative viticulture and winemaking of products that are more appealing to the market and the consumers. On the other hand, consumption of the food safety or organic products, and therefore of organic grapes and wine, is increasingly common in the world, in particular in Europe. The Regional Rural Development Standing Working Group (SWG RRD), as a regional intergovernmental organization gathers actors in the viticulture and winemaking sector from states and territories of the Western Balkans (South-East Europe) in the Expert Working Group for Wine, with the aim of improving viticulture and winemaking in this region through joint activities. In accordance with the aforementioned, the SWG RRD is working on advancing organic production of grapes and wine, and on recognition of specificities of the terroir of wine-growing areas in Western Balkans. In addition, as part of the project “Facilitation of Exchange and Advice on Wine Regulations in Western Balkan Countries” helmed by the German Federal Ministry of Food and Agriculture, in addition to harmonization of relevant legislation with EU regulations, efforts are being invested towards recognition of organic wines. Within activities and project implemented by this organization, expert analyses and scientific research of the terroir of Western Balkans were carried out, and some of the results are presented in this paper.

Revealing the Barossa zone sub-divisions through sensory and chemical analysis of Shiraz wine

The Barossa zone is arguably one of the most well-recognised wine producing regions in Australia and internationally; known mainly for the production of its distinct Shiraz wines. However, within the broad Barossa geographical delimitation, a variation in terroir can be perceived and is expressed as sensorial and chemical profile differences between wines. This study aimed to explore the sub-division classification across the Barossa region using chemical and sensory measurements. Shiraz grapes from 4 different vintages and different vineyards across the Barossa (2018, n = 69; 2019, n = 72; 2020, n = 79; 2021, n = 64) were harvested and made using a standardised small lot winemaking procedure. The analysis involved a sensory descriptive analysis with a highly trained panel and chemical measurement including basic chemistry (e.g. pH, TA, alcohol content, total SO2), phenolic composition, volatile compounds, metals, proline, and polysaccharides. The datasets were combined and analysed through an unsupervised, clustering analysis. Firstly, each vintage was considered separately to investigate any vintage to vintage variation. The datasets were then combined and analysed as a whole. The number of sub-divisions based on the measurements were identified and characterised with their sensory and chemical profile and some consistencies were seen between the vintages. Preliminary analysis of the sensory results showed that in most vintages, two major groups could be identified characterised with one group showing a fruit-forward profile and another displaying savoury and cooked vegetables characters. The exploration of distinct profiles arising from the Barossa wine producing region will provide producers with valuable information about the regional potential of their wine assisting with tools to increase their target market and reputation. This study will also provide a robust and comprehensive basis to determine the distinctive terroir characteristics which exist within the Barossa wine producing region.

Modelling vine water stress during a critical period and potential yield reduction rate in European wine regions: a retrospective analysis

Most European vineyards are managed under rainfed conditions, where seasonal water deficit has become increasingly important. The flowering-veraison phenophase represents an important period for vine response to water stress, which is seldomly thoroughly evaluated. Therefore, we aim to quantify the flowering-veraison water stress levels using Crop Water Stress Indicator (CWSI) over 1986–2015 for important European wine regions, and to assess the respective potential Yield Lose Rate (YLR). Additionally, we also investigate whether an advanced flowering-veraison phase may help alleviating the water stress with improved yield. A process-based grapevine model STICS is employed, which has been extensively calibrated for flowering and veraison stages using observed data at 38 locations with 10 different grapevine varieties. Subsequently, the model is being implemented at the regional level, considering site-specific calibration results and gridded climate and soil datasets. The findings suggest wine regions with stronger flowering-veraison CWSI tend to have higher potential YLR. However, contrasting patterns are found between wine regions in France-Germany-Luxembourg and Italy-Portugal-Spain. The former tends to have slight-to-moderate drought conditions (CWSI<0.5) and a negligible-to-moderate YLR (<30%), whereas the latter possesses severe-to-extreme CWSI (>0.5) and substantial YLR (>40%). Wine regions prone to a high drought risk (CWSI>0.75) are also identified, which are concentrated in southern Mediterranean Europe. An advanced flowering-veraison phase may have benefited from cooler temperatures and a higher fraction of spring precipitation in wine regions of Italy-Portugal-Spain, resulting in alleviated CWSI and moderate reductions of YLR. For those of France-Germany-Luxembourg, this can have reduced flowering-veraison precipitation, but prevalent alleviations of YLR are also found, possibly because of shifted phase towards a cooler growing season with reduced evaporative demands. Overall, such a retrospective analysis might provide new insights towards better management of seasonal water deficit for conventionally vulnerable Mediterranean wine regions, but also for relatively cooler and wetter Central European regions.

What are the optimal ranges and thresholds for berry solar radiation for flavonoid biosynthesis?

In wine grape production, canopy management practices are applied to control the source-sink balance and improve the cluster microclimate to enhance berry composition. The aim of this study was to identify the optimal ranges of berry solar radiation exposure (exposure) for upregulation of flavonoid biosynthesis and thresholds for their degradation, to evaluate how canopy management practices such as leaf removal, shoot thinning, and a combination of both affect the grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) yield components, berry composition, and flavonoid profile under context of climate change. First experiment assessed changes in the grape flavonoid content driven by four degrees of exposure. In the second experiment, individual grape berries subjected to different exposures were collected from two cultivars (Cabernet Sauvignon and Petit Verdot). The third experiment consisted of an experiment with three canopy management treatments (i) LR (removal of 5 to 6 basal leaves), (ii) ST (thinned to 24 shoots per vine), and (iii) LRST (a combination of LR and ST) and an untreated control (UNT). Berry composition, flavonoid content and profiles, and 3-isobutyl 2-methoxypyrazine were monitored during berry ripening. Although increasing canopy porosity through canopy management practices can be helpful for other purposes, this may not be the case of flavonoid compounds when a certain proportion of kaempferol was achieved. Our results revealed different sensitivities to degradation within the flavonoid groups, flavonols being the only monitored group that was upregulated by solar radiation. Within different canopy management practices, the main effects were due to the ST. Under environmental conditions given in this trial, ST and LRST hastened fruit maturity; however, a clear improvement of the flavonoid compounds (i.e., greater anthocyanin) was not observed at harvest. Methoxypyrazine berry content decreased with canopy management practices studied. Although some berry traits were improved (i.e. 2.5° Brix increase in berry total soluble solids) due to canopy management practices (ST), this resulted in a four-fold increase in labor operations cost, two-fold decrease in yield with a 10-fold increase in anthocyanin production cost per hectare that should be assessed together as the climate continues to get hot.

Influence of climatic conditions on grape composition of Tempranillo in La Mancha DO (Spain)

The aim of this work was to analyze the variability in grape composition of the Tempranillo cultivar related to climatic conditions, in La Mancha Designation of Origin. Grape composition (sugar content, total acidity, pH, malic acid, and total and extractable anthocyanins) recorded during ripening, were analysed for the period 2000-2019. The weather conditions at daily time scale, recorded during the same period, were also evaluated. The relationships between grape parameters with climatic variables related to temperature and to water deficits, referring different periods between phenological events along the growing cycle, were evaluated using regression analysis. High variability in grape composition was observed in the period analysed. Total acidity varied between 3.7 and 7.3 gL-1 while malic acid varied between 1.2 and 4 gL-1. The extractable anthocyanins ranged between 526 and 972 mgL-1, and total anthocyanins ranged between 922 and 1388 mgL-1, being the lowest values recorded in the hottest year (2017). Total acidity decreased 0.77 gL-1 for an increase of 100 GDD, while malic acid decrease in 0.42 gL-1 for the same GDD increase, being the period between veraison and harvest the one that seemed to have higher influence on acidity. In addition, it was confirmed that increasing water deficits decreased acidity. Total and extractable anthocyanins increased in about 210 and 105 mgL-1, respectively, with an increase of 100 GDD from veraison to harvest, and the increase in water deficits favour the increase of anthocyanins, both total and extractable anthocyanins. Total and extractable anthocyanins concentration increased in 35 and 22 mgL-1 per an increase of 10 mm in the water deficit. These results can be of interest to understand the potential changes that grapes composition may suffer under future warmer climates.