IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Impact of dosage sugar-type and ageing on finished sparkling wine composition and development of Maillard reaction-associated compounds

Impact of dosage sugar-type and ageing on finished sparkling wine composition and development of Maillard reaction-associated compounds

Abstract

The Maillard reaction (MR) is a non-enzymatic reaction between reducing sugars and amino acids, resulting in the production of volatile and flavour-active compounds. Existing MR research primarily addresses thermally processed foods, whereas limited studies have evaluated low-temperature MR environments including sparkling wine. Sparkling wine is produced in low temperature conditions (15 ± 3°C), with low pH (pH 3-3.4) and high acidity (titratable acidity 7-12 g/L) 1. Various MR species including furans, acryl amides, and heterocyclic amines have been identified in aged sparkling wines and contribute to roasted, caramel, and nutty aromas 2–4. The aim of this research was to investigate the composition of finished sparkling wine during 18-months of ageing by measuring the formation of target MR-associated compounds and the relative levels of precursor species (amino acids, reducing sugars). Variable liqueur de dosage sugar-types were assessed for their impact on MR-associated compounds during ageing. Liqueur de dosage (composed of sugar, wine, and SO2) is an addition made post-disgorgement, and these sugars may degrade or interact with amino acids, thereby influencing the formation of MR compounds. To the best of our knowledge, no prior literature has investigated the role of Liqueur de dosage in the MR. In this research, six dosage sugar treatments were evaluated including D-glucose, D-fructose, sucrose (cane-derived), sucrose (beet-derived), maltose, and commercial rectified grape must concentrate (RCGM), in addition to a zero-dosage/control (no sugar added). Treatments were carried out on 2015 vintage sparkling wine (3 years on lees; 59% Chardonnay, 41% Pinot Noir) produced by Niagara College Teaching Winery in Niagara-on-the-Lake, ON. Dosage treatments were prepared from the sparkling wine base to approximately 6 g/L residual sugar. Bottles were sealed with cork closures and cellared on-site at the Cool Climate Oenology & Viticulture Institute with environmental controls for temperature and humidity. At intervals of 0, 9 and 18-months post-dosage addition, triplicate bottles of each wine were chemically analyzed. MR-associated products were quantified by HS-SPME-GC-MS. Precursors including sugars and amino acids were quantified by enzymatic assay and NMR techniques, respectively, and sugar purity was determined by HPLC. After 18 months of aging post-disgorging, four MRPs showed concentration differences (p < 0.05) between dosage sugar treatments (ethyl 3-mercaptopropionate, furfuryl ethyl ether, 2-ethylthiazole, and 2-furyl methyl ketone). Changes in sugar and amino acid content during ageing were used to relate changes in MR compound formation with precursor consumption. This study establishes the effect of dosage sugar-type on the formation of volatile MR compounds in traditional method sparkling wines during ageing.

References

1. Kemp, B.; Alexandre, H.; Robillard, B.; Marchal, R. J. Agric. Food Chem. 2015, 63 (1), 19–38. 
2. Le Menn, N.; Marchand, S.; De Revel, G.; Demarville, D.; Laborde, D.; Marchal, R. J. Agric. Food Chem. 2017, 65 (11), 2345–2356. 
3. Keim, H.; De Revel, G.; Marchand, S.; Bertrand, A. J. Agric. Food Chem. 2002, 50 (21), 5803–5807. 
4. Marchand, S.; Almy, J.; de Revel, G. J. Food Sci. 2011, 76 (6), 861-868.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Article

Authors

Charnock Hannah1, Pickering Gary J.1,2,3,4, Kemp Belinda S.1,2

1Department of Biological Sciences, Faculty of Mathematics & Science, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON, Canada, L2S 3A1
2Cool Climate Oenology & Viticulture Institute, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON, Canada, L2S 3A1
3National Wine and Grape Industry Center, Charles Sturt University, McKeown Drive, Wagga Wagga, NSW 2678, Australia
4Sustainability Research Centre, University of the Sunshine Coast, 90 Sippy Downs Drive, Sippy Downs, QLD 4556, Australia

Contact the author

Keywords

sparkling wine, Maillard reaction, time-course ageing

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Rapid damage assessment and grapevine recovery after fire

There is increasing scientific consensus that climate changeis the underlying cause of the prolonged dry and hot conditions that have increased the risk of extreme fire weather in many countries around the world. In December 2019, a bushfire event occurred in the Adelaide Hills, South Australia where 25,000 hectares were burnt and in vineyards and surrounding areas various degrees of scorching and infrastructure damage occurred. The ability to coordinate and plan recovery after a fire event relies on robust and timely data. The current practice for measuring the scale and distribution of fire damage is to walk or drive the vineyard and score individual vines based on visual observation. The process is time consuming, subjective, or semi-quantitative at best. After the December 2019 fires, it took many months to access properties and estimate the area of vineyard damaged. This study compares the rapid assessment and mapping of fire damage using high-resolution satellite imagery with more traditional ground based measures. Satellite imagery tracking vineyard recovery in the season following the bushfire is being correlated to field assessments of vineyard productivity such as canopy health and development, fertility and carbohydrate storage. Canopy health in the seasons following the fires correlated to the severity of the initial fire damage. Severely damaged vines had reduced canopy growth, were infertile or had very low fertility as well as lower carbohydrate levels in buds and canes during dormancy, which reduced productivity in the seasons following the bushfire event. In contrast, vines that received minor damage were able to recover within 1-2 years. Tools that rapidly and affordably capture the extent and severity of damage over large vineyard area will allow producers, government and industry bodies to manage decisions in relation to fire recovery planning, coordination and delivery, improving the efficiency and effectiveness of their response.

Climate change impacts: a multi-stress issue

With the aim of producing premium wines, it is admitted that moderate environmental stresses may contribute to the accumulation of compounds of interest in grapes. However the ongoing climate change, with the appearance of more limiting conditions of production is a major concern for the wine industry economic. Will it be possible to maintain the vineyards in place, to preserve the current grape varieties and how should we anticipate the adaptation measures to ensure the sustainability of vineyards? In this context, the question of the responses and adaptation of grapevine to abiotic stresses becomes a major scientific issue to tackle. An abiotic stress can be defined as the effect of a specific factor of the physico-chemical environment of the plants (temperature, availability of water and minerals, light, etc.) which reduces growth, and for a crop such as the vine, the yield, the composition of the fruits and the sustainability of the plants. Water stress is in many minds, but a systemic vision is essential for at least two reasons. The first reason is that in natural environments, a single factor is rarely limiting, and plants have to deal with a combination of constraints, as for example heat and drought, both in time and at a given time. The second reason is that plants, including grapevine, have central mechanisms of stress responses, as redox regulatory pathways, that play an important role in adaptation and survival. Here we will review the most recent studies dealing with this issue to provide a better understanding of the grapevine responses to a combination of environmental constraints and of the underlying regulatory pathways, which may be very helpful to design more adapted solutions to cope with climate change.

Rootstock regulation of scion phenotypes: the relationship between rootstock parentage and petiole mineral concentration

Grapevine is grown as a graft since the end of the 19th century. Rootstocks not only provide tolerance to Phylloxera but also ensure the supply of water and mineral nutrients to the scion. Rootstocks are an important mean of adaptation to environmental conditions, because the scion controls the typical features of the grapes and wine. However, among the large diversity of rootstocks worldwide, few of them are commercially used in the vineyard. The aim of this study was to investigate the extent to which rootstocks modify the mineral composition of the petioles of the scion. Vitis vinifera cvs. Cabernet-Sauvignon, Pinot noir, Syrah and Ugni blanc were grafted onto 55 different rootstock genotypes and planted in a vineyard as three replicates of 5 vines. Petioles were collected in the cluster zone with 6 replicates per combination. Petiolar concentrations of 13 mineral elements (N, P, K, S, Mg, Ca, Na, B, Zn, Mn, Fe, Cu, Al) at veraison were determined. Scion, rootstock and the interaction explained the same proportion of the phenotypic variance for most mineral elements. Rootstock genotype showed a significant influence on the petiole mineral element composition. Rootstock effect explained from 7 % for Cu to 25 % for S of the variance. The difference of rootstock conferred mineral status is discussed in relation to vigor and fertility. Rootstocks were also genotyped with 23 microsatellite markers. Data were analysed according to genetic groups in order to determine whether the petiole mineral composition could be related to the genetic parentage of the rootstock. Thanks to a highly powerful design, it is the first time that such a large panel of rootstocks grafted with 4 scions has been studied. These results give the opportunity to better characterize the rootstocks and to enlarge the diversity used in the vineyard.

Water deficit differentially impacts the performances and the accumulation of grape metabolites of new varieties tolerant to fungi

The use of resistant varieties is a long-term but promising solution to reduce chemical input in viticulture. Several important breeding programs in Europe and abroad are now releasing a range of new hybrids performing well regarding fungi susceptibility and producing good quality wines. Unfortunately, insufficient attention is paid by the breeders to the adaptation of these varieties to climatic changes, notably to the increased climatic demand and water deficit (WD). Thus, prior to the adoption of such varieties by the wine industry in Mediterranean regions, there is a need to consider their suitability to WD. This study aimed to characterize the different drought-strategies adopted by 6 new resistant varieties selected by INRAE in comparison to Syrah. To allow the assessment of long-term impacts of WD, field-grown vines were exposed to contrasted WD from 2018 to 2021 under a semi-arid Mediterranean climate. A gradient of WD was applied in the field and controlled through plant measurements at the single plant level. Grape development was non-destructively monitored to determine the arrest of berry phloem unloading. The impacts of WD on berry composition, including water, primary metabolites (sugars, organic acids), secondary metabolites (anthocyanins, thiols precursors) and main cations contents, were assessed at this specific stage. Results showed different varietal responses during the year and inter-annual acclimation in terms of plant water use efficiency, biomass accumulation, as well as yield components and berry composition. WD differentially reduced the accumulation of primary metabolites at plant and berry levels, but it little changed their concentrations in the fruits at the ripe stage. Moreover, WD differentially impacted the accumulation of secondary metabolites and major cations between the varieties. In the talk, we’ll present the main results regarding the WD impacts on fruit metabolites and enlarge the reflection about the practical assessment of the grapevine acclimation to WD.

Effect of fertigation strategies to adapt PGI Côtes de Gascogne production to hot vintage

The development of fertigation could be a possible solution to adapt PGI Côtes de Gascogne (south-western France) wine production to climate change. The goal would be to limit the negative effects of water stress on yield performance expectation (around 15 tons per hectare) and to make the use of fertilizers more efficient. This study aimed to compare the effects of three strategies of water and minerals supply on grapes and wines qualities. Two fertigation practices were compared to a rainfed control which is the current standard of the local grape growing production. The fertilizers (nitrogen and potassium) were (i) fully brought by irrigation pipe during the season, (ii) partially brought by irrigation pipe and partially on the soil or (iii) fully brought on the soil at the beginning of the season for the non-irrigated control (local standard). The trial was run on cv. Colombard trained on spur pruned with vertical shoot positioning system on a sandy-silty-clay soil over the 2020 vintage which was particularly hot for the region. Moderate to strong water deficit appeared during the growing period of the berries and held on after veraison. Irrigation strategies allowed for maintaining grapevine without water deficit and being significantly different from the control water status. Grapevine with fully or partial fertigation strategies produced 25% more yield mainly due to the increase of the bunch weight. Also, the fully fertigation showed the best ratio between yield and maturity and brought 30% less of fertilizers (both nitrogen and potassium) than the two other strategies. Finally, the analysis of aromatic compounds in Colombard wines, varietal thiols family, showed the same level of concentrations for the 3 treatments, confirming that the yield performance did not impact the aromatic potential in this trial.