IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Composition and molar mass distribution of different must and wine colloids

Composition and molar mass distribution of different must and wine colloids

Abstract

A major problem for winemakers is the formation of proteinaceous haze after bottling. Although the exact mechanisms remain unclear, this haze is formed by unfolding and agglomeration of grape proteins, being additionally influenced by numerous further factors. For instance, increased levels of polyphenols and sulfate ions, high pH and ionic strength, and increased storage temperatures have been discussed to promote haze formation. In contrast, organic acids and polysaccharides appear to inhibit protein agglomeration (Albuquerque et al. 2021). To avoid haze formation, winemakers use bentonite to reduce protein levels in the wine before bottling. However, the bentonite treatment imposes negative side effects such as losses in wine quantity and quality, as well as costs of bentonite waste disposal (van Sluyter et al. 2015). To better understand haze formation and to find alternative procedures for protein removal e.g. by enzymatic treatments, detailed insights into the composition of the wine colloids might be helpful.
Prior to characterization, colloids were isolated from five different musts (four varieties from five vineyards, three with pectinase treatment) and their corresponding wines by ultrafiltration (10 kDa cut-off) and freeze-drying. Protein and carbohydrate composition were determined after hydrolysis by ion chromatography and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), respectively. Molar mass distribution of colloids was determined by size exclusion chromatography with multi angle light scattering in combination with an UV and RI detector (SEC-UV-MALS-RI).
Colloids were found to contain a wide range of 8.9 to 67.1 g protein and 28.1 to 78.0 g carbohydrates per 100 g dry matter. Thus, protein concentrations in must and wine were been between 0.06 and 0.40 g/L and carbohydrate concentrations between 0.17 and 0.65 g/L. While there were just minor differences in the amino acid composition between the musts and wines, the carbohydrate composition was different in the samples. For instance, arabinose and galactose were the main sugars found in all hydrolyzed must colloids, while galacturonic acid was present in higher amounts in those not treated with pectinase. After fermentation, mannose was found to be the main sugar in hydrolyzed wine colloids. SEC-UV-MALS-RI showed that the colloids contained three main fractions. Two carbohydrate-rich fractions with average molar masses from 931 to 22,617 kDa and from 80 to 495 kDa as well as a proteinaceous fraction with an average molar mass between 16 to 44 kDa.
Our results indicate that colloid concentration and composition in wine is heavily influenced by variety, vineyard and oenological practices. The isolated colloids and the analytical methods will in the future be used to screen for enzyme preparations suitable to degrade proteins in must and wine to avoid haze formation.

References

Albuquerque, Wendell; Seidel, Leif; Zorn, Holger; Will, Frank; Gand, Martin (2021): Haze Formation and the Challenges for Peptidases in Wine Protein Fining. In: Journal of Agricultural and Food Chemistry 69, S. 14402–14414.
van Sluyter, Steven C.; McRae, Jacqui M.; Falconer, Robert J.; Smith, Paul A.; Bacic, Antony; Waters, Elizabeth J.; Marangon, Matteo (2015): Wine Protein Haze: Mechanisms of Formation and Advances in Prevention. In: Journal of Agricultural and Food Chemistry 63 (16), S. 4020–4030.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Seidel Leif1, Albuquerque Wendell2, Happel Katharina3, Gand Martin2, Zorn Holger2,3, Schweiggert Ralf1 and Will Frank1

1Department of Beverage Research, Geisenheim University
2Institute of Food Chemistry and Food Biotechnology, Justus Liebig Giessen 
3Fraunhofer Institute for Molecular Biology and Applied Ecology, Giessen, Germany

Contact the author

Keywords

wine colloids, proteins, carbohydrates, molar mass

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

How can historical cultivars mitigate the effects of climate change?

IFV, INRAe and the national network “Partenaires de la Sélection Vigne” representing 37 organizations from the different wine regions, have been working increasingly closely over the last 2 decades towards the preservation of the French varietal patrimony. There are approximately 600 patrimonial varieties according to INRAe and SupAgro Montpellier experts, including ancient cultivars (400) and intravarietal crossbreeds obtained since the 19th century. In the context of a drastic reduction in such varieties from the mid 1980’s in favor of mainstream varieties, it was essential to carry out an inventory of old vines and vineyards. INRAe Vassal collection plays a key role here as it holds the largest diversity available, along with a rich bibliography and herbariums, offering us the opportunity to document and double check the identity of a cultivar, consolidating the expertise of ampelographers. The work is carried out in several stages, from verifying the existence of a variety in a small region, through to rehabilitation. During this session, the authors present the process that leads to the official registration of a variety. After this, IFV selection center takes over to initiate the process of selection and propagation. A specific focus within regions such as the Alps, Champagne and the South-West will provide details of the full procedure. Bia, Bouysselet, Chardonnay rose, Mecle and the aptly named Tardif, are some of the cultivars that have followed this procedure. Furthermore, a recent regulation established by INAO on “varieties of interest for adaptation purposes” might boost uptake by growers. Since 2006, 36 historical cultivars have been registered. Most of these have been neglected in the past due to late maturity, lack of sugar and high titratable acidity at harvest time. Such characteristics are today considered as positive qualities, not only in mitigation of the effects of climate change, but also as an opportunity for restoring diversity…

Influence of climatic conditions on grape composition of Tempranillo in La Mancha DO (Spain)

The aim of this work was to analyze the variability in grape composition of the Tempranillo cultivar related to climatic conditions, in La Mancha Designation of Origin. Grape composition (sugar content, total acidity, pH, malic acid, and total and extractable anthocyanins) recorded during ripening, were analysed for the period 2000-2019. The weather conditions at daily time scale, recorded during the same period, were also evaluated. The relationships between grape parameters with climatic variables related to temperature and to water deficits, referring different periods between phenological events along the growing cycle, were evaluated using regression analysis. High variability in grape composition was observed in the period analysed. Total acidity varied between 3.7 and 7.3 gL-1 while malic acid varied between 1.2 and 4 gL-1. The extractable anthocyanins ranged between 526 and 972 mgL-1, and total anthocyanins ranged between 922 and 1388 mgL-1, being the lowest values recorded in the hottest year (2017). Total acidity decreased 0.77 gL-1 for an increase of 100 GDD, while malic acid decrease in 0.42 gL-1 for the same GDD increase, being the period between veraison and harvest the one that seemed to have higher influence on acidity. In addition, it was confirmed that increasing water deficits decreased acidity. Total and extractable anthocyanins increased in about 210 and 105 mgL-1, respectively, with an increase of 100 GDD from veraison to harvest, and the increase in water deficits favour the increase of anthocyanins, both total and extractable anthocyanins. Total and extractable anthocyanins concentration increased in 35 and 22 mgL-1 per an increase of 10 mm in the water deficit. These results can be of interest to understand the potential changes that grapes composition may suffer under future warmer climates.

Elevational range shifts of mountain vineyards: Recent dynamics in response to a warming climate

Increasing temperatures worldwide are expected to cause a change in spatial distribution of plant species along elevational gradients and there are already observable shifts to higher elevations as a consequence of climate change for many species. Not only naturally growing plants, but also agricultural cultivations are subject to the effects of climate change, as the type of cultivation and the economic viability depends largely on the prevailing climatic conditions. A shift to higher elevations therefore represents a viable adaptation strategy to climate change, as higher elevations are characterized by lower temperatures. This is especially important in the case of viticulture because a certain wine-style can only be achieved under very specific climatic conditions. Although there are several studies investigating climatic suitability within winegrowing regions or longitudinal shifts of winegrowing areas, little is known about how fast vineyards move to higher elevations, which may represent a viable strategy for winegrowers to maintain growing conditions and thus wine-style, despite the effects of climate change. We therefore investigated the change in the spatial distribution of vineyards along an elevational gradient over the past 20 years in the mountainous wine-growing region of Alto Adige (Italy). A dataset containing information about location and planting year of more than 26000 vineyard parcels and 30 varieties was used to perform this analysis. Preliminary results suggest that there has been a shift to higher elevations for vineyards in general (from formerly 700m to currently 850 m a.s.l., with extreme sites reaching 1200 m a.s.l.), but also that this development has not been uniform across different varieties and products (i.e. vitis vinifera vs hybrid varieties and still vssparkling wines). This is important for climate change adaptation as well as for rural development. Mountain areas, especially at mid to high elevations, are often characterized by severe land abandonment which can be avoided to some degree if economically viable and sustainable land management strategies are available.

Mapping and tracking canopy size with VitiCanopy

Understanding vineyard variability to target management strategies, apply inputs efficiently and deliver consistent grape quality to the winery is essential. However, despite inherent vineyard variability, the majority are managed as if they are uniform. VitiCanopy is a simple, grower-friendly tool for precision/digital viticulture that allows users to collect and interpret objective spatial information about vineyard performance. After four years of field and market research, an upgraded VitiCanopy has been created to achieve a more streamlined, technology-assisted vine monitoring tool that provides users with a set of superior new features, which could significantly improve the way users monitor their grapevines. These new features include:
• New user interface
• User authentication
• Batch analysis of multiple images
• Ease the learning curve through enhanced help features
• Reporting via the creation of colour maps that will allow users to assess the spatial differences in canopies within a vineyard.
Use-case examples are presented to demonstrate the quantification and mapping of vineyard variability through objective canopy measurements, ground-truthing of remotely sensed measurements, monitoring of crop conditions, implementation of disease and water management decisions as well as creating a history of each site to forecast quality. This intelligent tool allows users to manage grapevines and make informed management choices to achieve the desired production targets and remain profitable.

Drought effect on aromatic and phenolic potential of seven recovered grapevine varieties in Castilla-La Mancha region (Spain)

The effects of climate change are seriously affecting the quality of wine grapes. High temperatures and drought cause imbalances in the chemical composition of grapes. The result is overripe grapes with low acidity and high sugar content, which produce wines with excessive alcohol content, lacking in freshness and not very aromatic. As a consequence, the search of varieties with capacity of produce quality grapes in adverse climate conditions is a good alternative to preserve the sustainability of vineyards. In this work, quality parameters of seven Vitis vinifera L. cultivars (five whites and two reds) recently recovered from extinction and grown under two different hydric regimes (rainfed and irrigated) were analyzed during the 2020 vintage. At harvest time, weight of 100 berries, must physicochemical parameters (brix degree, total acidity, malic acid, pH), and carbon and oxygen isotope ratios (δ13C, δ18O) were determined. Subsequently, varietal aroma potential index (IPAv) and total polyphenol index (TPI) were analyzed. Quality parameters, IPAv and TPI, showed significant differences between varieties and water regimes. Both red varieties, Moribel and Tinto Fragoso, stood out for their high aromatic and phenolic potential, which was higher under rainfed regime. Regarding to white varieties, Montonera del Casar and Jarrosuelto stood out in terms of varietal aroma potential. Montonera del Casar high acidity in its musts and Jarrosuelto showed the highest berry weights.