IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Composition and molar mass distribution of different must and wine colloids

Composition and molar mass distribution of different must and wine colloids

Abstract

A major problem for winemakers is the formation of proteinaceous haze after bottling. Although the exact mechanisms remain unclear, this haze is formed by unfolding and agglomeration of grape proteins, being additionally influenced by numerous further factors. For instance, increased levels of polyphenols and sulfate ions, high pH and ionic strength, and increased storage temperatures have been discussed to promote haze formation. In contrast, organic acids and polysaccharides appear to inhibit protein agglomeration (Albuquerque et al. 2021). To avoid haze formation, winemakers use bentonite to reduce protein levels in the wine before bottling. However, the bentonite treatment imposes negative side effects such as losses in wine quantity and quality, as well as costs of bentonite waste disposal (van Sluyter et al. 2015). To better understand haze formation and to find alternative procedures for protein removal e.g. by enzymatic treatments, detailed insights into the composition of the wine colloids might be helpful.
Prior to characterization, colloids were isolated from five different musts (four varieties from five vineyards, three with pectinase treatment) and their corresponding wines by ultrafiltration (10 kDa cut-off) and freeze-drying. Protein and carbohydrate composition were determined after hydrolysis by ion chromatography and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), respectively. Molar mass distribution of colloids was determined by size exclusion chromatography with multi angle light scattering in combination with an UV and RI detector (SEC-UV-MALS-RI).
Colloids were found to contain a wide range of 8.9 to 67.1 g protein and 28.1 to 78.0 g carbohydrates per 100 g dry matter. Thus, protein concentrations in must and wine were been between 0.06 and 0.40 g/L and carbohydrate concentrations between 0.17 and 0.65 g/L. While there were just minor differences in the amino acid composition between the musts and wines, the carbohydrate composition was different in the samples. For instance, arabinose and galactose were the main sugars found in all hydrolyzed must colloids, while galacturonic acid was present in higher amounts in those not treated with pectinase. After fermentation, mannose was found to be the main sugar in hydrolyzed wine colloids. SEC-UV-MALS-RI showed that the colloids contained three main fractions. Two carbohydrate-rich fractions with average molar masses from 931 to 22,617 kDa and from 80 to 495 kDa as well as a proteinaceous fraction with an average molar mass between 16 to 44 kDa.
Our results indicate that colloid concentration and composition in wine is heavily influenced by variety, vineyard and oenological practices. The isolated colloids and the analytical methods will in the future be used to screen for enzyme preparations suitable to degrade proteins in must and wine to avoid haze formation.

References

Albuquerque, Wendell; Seidel, Leif; Zorn, Holger; Will, Frank; Gand, Martin (2021): Haze Formation and the Challenges for Peptidases in Wine Protein Fining. In: Journal of Agricultural and Food Chemistry 69, S. 14402–14414.
van Sluyter, Steven C.; McRae, Jacqui M.; Falconer, Robert J.; Smith, Paul A.; Bacic, Antony; Waters, Elizabeth J.; Marangon, Matteo (2015): Wine Protein Haze: Mechanisms of Formation and Advances in Prevention. In: Journal of Agricultural and Food Chemistry 63 (16), S. 4020–4030.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Seidel Leif1, Albuquerque Wendell2, Happel Katharina3, Gand Martin2, Zorn Holger2,3, Schweiggert Ralf1 and Will Frank1

1Department of Beverage Research, Geisenheim University
2Institute of Food Chemistry and Food Biotechnology, Justus Liebig Giessen 
3Fraunhofer Institute for Molecular Biology and Applied Ecology, Giessen, Germany

Contact the author

Keywords

wine colloids, proteins, carbohydrates, molar mass

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Optimizing stomatal traits for future climates

Stomatal traits determine grapevine water use, carbon supply, and water stress, which directly impact yield and berry chemistry. Breeding for stomatal traits has the strong potential to improve grapevine performance under future, drier conditions, but the trait values that breeders should target are unknown. We used a functional-structural plant model developed for grapevine (HydroShoot) to determine how stomatal traits impact canopy gas exchange, water potential, and temperature under historical and future conditions in high-quality and hot-climate California wine regions (Napa and the Central Valley). Historical climate (1990-2010) was collected from weather stations and future climate (2079-99) was projected from 4 representative climate models for California, assuming medium- and high-emissions (RCP 4.5 and 8.5). Five trait parameterizations, representing mean and extreme values for the maximum stomatal conductance (gmax) and leaf water potential threshold for stomatal closure (Ψsc), were defined from meta-analyses. Compared to mean trait values, the water-spending extremes (highest gmax or most negative Ysc) had negligible benefits for carbon gain and canopy cooling, but exacerbated vine water use and stress, for both sites and climate scenarios. These traits increased cumulative transpiration by 8 – 17%, changed cumulative carbon gain by -4 – 3%, and reduced minimum water potentials by 10 – 18%. Conversely, the water-saving extremes (lowest gmax or least negative Ψsc) strongly reduced water use and stress, but potentially compromised the carbon supply for ripening. Under RCP 8.5 conditions, these traits reduced transpiration by 22 – 35% and carbon gain by 9 – 16% and increased minimum water potentials by 20 – 28%, compared to mean values. Overall, selecting for more water-saving stomatal traits could improve water-use efficiency and avoid the detrimental effects of highly negative canopy water potentials on yield and quality, but more work is needed to evaluate whether these benefits outweigh the consequences of minor declines in carbon gain for fruit production.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Pruned vine biomass exclusion from a clay loam vineyard soil – examining the impact on physical/chemical properties

The wine industry worldwide faces increasing challenges to achieve sustainable levels of carbon emission mitigation. This project seeks to establish the feasibility of harvesting winter pruned vineyard biomass (PVB) for potential use in carbon footprint reduction, through its use as a renewable biofuel for energy production. In order to make this recommendation, technical issues such as the potential environmental impact, chemical composition and fuel suitability, and logistical challenges of harvesting biomass needs to be understood to compare with the results from similar studies. Of particular interest is the role PVB plays as a carbon source in vineyard soils and what effect annual removal might have on soil carbon sequestration. A preliminary trial was established in the Waite Campus vineyard (University of Adelaide) to test current management strategies. Vines are grown in a Eutrophic, Red Dermosol clay loam soil with well managed midrow swards. A comparison was undertaken of mid-row treatments in two 0.25 Ha blocks (Shiraz and Semillon), including annual cultivation for seed bed preparation, the deliberate exclusion of PVB (25 years) and incorporation of PVB (13 years) at an average of 3.4 and 5.5 Mg/Ha-1 for Shiraz and Semillon respectively. In both 0-10cm and 10-30cm soil core sample depths, combined soil carbon % measures in the desired range of 1.80 to 3.50, were not significantly different between treatments or cultivars and yielded an estimated 42 Mg/ha-1 of sequestered soil carbon. Other key physical and chemical measures were likewise not significantly different between treatments. Preliminary results suggest that in a temperate zone vineyard, managed such as the one used in this study, there is no long term negative impact on soil carbon sequestration through removing PVB. This implies that growers could confidently harvest PVB for use in several end fates including as a bio fuel.

Analysis of some environmental factors and cultural practices that affect the production and quality of the Manto Negro, Callet and Prensal Blanc varieties

45 non irrigated vineyards distributed in the DO (Denomination) Pla i Llevant de Mallorca and the DO Binissalem Mallorca were used to investigate the characteristics of production and quality and their relationships certain environmental factors and cultural practices. The grape varieties investigated are autochthonous to the island of Mallorca, Manto Negro and Callet as red and Prensal Blanc as white. All plants were measured for four consecutive years in the main production and quality parameters. Among the environmental factors, the type of soil has been studied, more specifically its water retention capacity, the planting density, the age of the vineyard and the level of viral infection. The presence or absence of virus seems to have no effect on any component studied in the varieties studied. For the white variety Prensal Blanc age is negatively correlated with production and the number of bunches, nevertheless it does not cause any effect on the required quality parameters. However, for the red varieties Callet and Manto Negro, the age of the plantation is the variable that best correlates with the quality parameters, therefore the old vines should be the object of preservation by the viticulturists and winemakers in order to guarantee its contribution to the quality of the wines made with these varieties.

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…