IVAS 2022 banner
IVES 9 IVES Conference Series 9 IVAS 9 IVAS 2022 9 Composition and molar mass distribution of different must and wine colloids

Composition and molar mass distribution of different must and wine colloids

Abstract

A major problem for winemakers is the formation of proteinaceous haze after bottling. Although the exact mechanisms remain unclear, this haze is formed by unfolding and agglomeration of grape proteins, being additionally influenced by numerous further factors. For instance, increased levels of polyphenols and sulfate ions, high pH and ionic strength, and increased storage temperatures have been discussed to promote haze formation. In contrast, organic acids and polysaccharides appear to inhibit protein agglomeration (Albuquerque et al. 2021). To avoid haze formation, winemakers use bentonite to reduce protein levels in the wine before bottling. However, the bentonite treatment imposes negative side effects such as losses in wine quantity and quality, as well as costs of bentonite waste disposal (van Sluyter et al. 2015). To better understand haze formation and to find alternative procedures for protein removal e.g. by enzymatic treatments, detailed insights into the composition of the wine colloids might be helpful.
Prior to characterization, colloids were isolated from five different musts (four varieties from five vineyards, three with pectinase treatment) and their corresponding wines by ultrafiltration (10 kDa cut-off) and freeze-drying. Protein and carbohydrate composition were determined after hydrolysis by ion chromatography and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), respectively. Molar mass distribution of colloids was determined by size exclusion chromatography with multi angle light scattering in combination with an UV and RI detector (SEC-UV-MALS-RI).
Colloids were found to contain a wide range of 8.9 to 67.1 g protein and 28.1 to 78.0 g carbohydrates per 100 g dry matter. Thus, protein concentrations in must and wine were been between 0.06 and 0.40 g/L and carbohydrate concentrations between 0.17 and 0.65 g/L. While there were just minor differences in the amino acid composition between the musts and wines, the carbohydrate composition was different in the samples. For instance, arabinose and galactose were the main sugars found in all hydrolyzed must colloids, while galacturonic acid was present in higher amounts in those not treated with pectinase. After fermentation, mannose was found to be the main sugar in hydrolyzed wine colloids. SEC-UV-MALS-RI showed that the colloids contained three main fractions. Two carbohydrate-rich fractions with average molar masses from 931 to 22,617 kDa and from 80 to 495 kDa as well as a proteinaceous fraction with an average molar mass between 16 to 44 kDa.
Our results indicate that colloid concentration and composition in wine is heavily influenced by variety, vineyard and oenological practices. The isolated colloids and the analytical methods will in the future be used to screen for enzyme preparations suitable to degrade proteins in must and wine to avoid haze formation.

References

Albuquerque, Wendell; Seidel, Leif; Zorn, Holger; Will, Frank; Gand, Martin (2021): Haze Formation and the Challenges for Peptidases in Wine Protein Fining. In: Journal of Agricultural and Food Chemistry 69, S. 14402–14414.
van Sluyter, Steven C.; McRae, Jacqui M.; Falconer, Robert J.; Smith, Paul A.; Bacic, Antony; Waters, Elizabeth J.; Marangon, Matteo (2015): Wine Protein Haze: Mechanisms of Formation and Advances in Prevention. In: Journal of Agricultural and Food Chemistry 63 (16), S. 4020–4030.

DOI:

Publication date: June 23, 2022

Issue: IVAS 2022

Type: Poster

Authors

Seidel Leif1, Albuquerque Wendell2, Happel Katharina3, Gand Martin2, Zorn Holger2,3, Schweiggert Ralf1 and Will Frank1

1Department of Beverage Research, Geisenheim University
2Institute of Food Chemistry and Food Biotechnology, Justus Liebig Giessen 
3Fraunhofer Institute for Molecular Biology and Applied Ecology, Giessen, Germany

Contact the author

Keywords

wine colloids, proteins, carbohydrates, molar mass

Tags

IVAS 2022 | IVES Conference Series

Citation

Related articles…

Upscaling the integrated terroir zoning through digital soil mapping: a case study in the Designation of Origin Campo de Borja

homogeneous zones by intersecting several partial zonings of major factors that influence vineyard growth. Each of them follows specific process from their corresponding disciplines. Soil zoning specifically refers to a Soil Resource Inventory map that has traditionally been generated by conventional soil mapping methods. These methods have shortcomings in reaching fine cartographic and categorical details and involve significant expenses, which undermines their applicability. A new framework named Digital Soil Mapping has introduced quantitative models by statistical techniques to establish soil-landscape relationships and is able to provide intensive scale cartography.

In the present study, a microzoning at 1:10.000 scale is generated from an initial zoning, where the conventional soil map with polytaxic map units is replaced by a new one from digital techniques that disaggregates them. The comparison between the zonings considers a quantitative evaluation of capability for each Homogeneous Terroir Unit by means of the Viticultural Quality Index and its categorization based on its distribution by map. The spatial intersection of both maps gives rise to a confusion matrix in which the flows of class variations after the substitution are assessed.

The results show a five-fold increase in the number of Homogeneous Terroir Units identified and a larger differentiation among them, evidenced by a wider range in the capability index distribution. Both elements are accompanied by an increase in the detection of areas of higher potential within previously undervalued uniform zones.These features are a direct effect of the improvements brought by Digital Soil Mapping techniques and would verify the advantages of their implementation in the Integrated Terroir zoning. Eventually, such new highly detailed terroir units would benefit precision viticulture and sustainable management practices.

Understanding graft union formation by using metabolomic and transcriptomic approaches during the first days after grafting in grapevine

Since the arrival of Phyloxera (Daktulosphaira vitifolia) in Europe at the end of the 19th century, grafting has become essential to cultivate Vitis vinifera. Today, grafting provides not only resistance to this aphid, but it used to adapt the cultivars according to the type of soil, environment, or grape production requirements by using a panel of rootstocks. As part of vineyard decline, it is often mentioned the importance of producing quality grafted grapevine to improve vineyard longevity, but, to our knowledge, no study has been able to demonstrate that grafting has a role in this context. However, some scion/rootstock combinations are considered as incompatible due to poor graft union formation and subsequently high plant mortality soon after grafting. In a context of climate change where the creation of new cultivars and rootstocks is at the centre of research, the ability of new cultivars to be grafted is therefore essential. The early identification of graft incompatibility could allow the selection of non-viable plants before planting and would have a beneficial impact on research and development in the nursery sector. For this reason, our studies have focused on the identification of metabolic and transcriptomic markers of poor grafting success during the first days/week after grafting; we have identified some correlations between some specialized metabolites, especially stilbenes, and grafting success, as well as an accumulation of some amino acids in the incompatible combination. The study of the metabolome and the transcriptome allowed us to understand and characterise the processes involved during graft union formation.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.

Use of a new, miniaturized, low-cost spectral sensor to estimate and map the vineyard water status from a mobile 

Optimizing the use of water and improving irrigation strategies has become increasingly important in most winegrowing countries due to the consequences of climate change, which are leading to more frequent droughts, heat waves, or alteration of precipitation patterns. Optimized irrigation scheduling can only be based on a reliable knowledge of the vineyard water status.

In this context, this work aims at the development of a novel methodology, using a contactless, miniaturized, low-cost NIR spectral tool to monitor (on-the-go) the vineyard water status variability. On-the-go spectral measurements were acquired in the vineyard using a NIR micro spectrometer, operating in the 900–1900 nm spectral range, from a ground vehicle moving at 3 km/h. Spectral measurements were collected on the northeast side of the canopy across four different dates (July 8th, 14th, 21st and August 12th) during 2021 season in a commercial vineyard (3 ha). Grapevines of Vitis vinifera L. Graciano planted on a VSP trellis were monitored at solar noon using stem water potential (Ψs) as reference indicators of plant water status. In total, 108 measurements of Ψs were taken (27 vines per date).

Calibration and prediction models were performed using Partial Least Squares (PLS) regression. The best prediction models for grapevine water status yielded a determination coefficient of cross-validation (r2cv) of 0.67 and a root mean square error of cross-validation (RMSEcv) of 0.131 MPa. This predictive model was employed to map the spatial variability of the vineyard water status and provided useful, practical information towards the implementation of appropriate irrigation strategies. The outcomes presented in this work show the great potential of this low-cost methodology to assess the vineyard stem water potential and its spatial variability in a commercial vineyard.

Low-cost sensors as a support tool to monitor soil-plant heat exchanges in a Mediterranean vineyard

Mediterranean viticulture is increasingly exposed to more frequent extreme conditions such as heat waves. These extreme events co-occur with low soil water content, high air vapor pressure deficit and high solar radiant energy fluxes and result in leaf and berry sunburn, lower yield, and berry quality, which is a major constraint for the sustainability of the sector. Grape growers must find ways to proper and effectively manage heat waves and extreme canopy and berry temperatures. Irrigation to keep soil moisture levels and enable adequate plant turgor, and convective and evaporative cooling emerged as a key tool to overcome this major challenge. The effects of irrigation on soil and plant water status are easily quantifiable but the impact of irrigation on soil and canopy temperature and on heat convection from soil to cluster zone remain less characterized. Therefore, a more detailed quantification of vineyard heat fluxes is highly relevant to better understand and implement strategies to limit the effects of extreme weather events on grapevine leaf and berry physiology and vineyards performance. Low-cost sensor technologies emerge as an opportunity to improve monitoring and support decision making in viticulture. However, validation of low-cost sensors is mandatory for practical applicability. A two-year study was carried in a vineyard in Alentejo, south of Portugal, using low-cost thermal cameras (FLIR One, 80×60 pixels and FLIR C5, 160×120 pixels, 8-14 µm, FLIR systems, USA) and pocket thermohygrometers (Extech RHT30, EXTECH instruments, USA) to monitor grapevine and soil temperatures. Preliminary results show that low-cost cameras can detect severe water stress and support the evaluation of vertical canopy temperature variability, providing information on soil surface temperature. All these thermal parameters can be relevant for soil and crop management and be used in decision support systems.